刘继红
从事果树逆境生理与分子生物学研究
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- 姓名:刘继红
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学术头衔:
博士生导师, 教育部“新世纪优秀人才支持计划”入选者
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学科领域:
果树学
- 研究兴趣:从事果树逆境生理与分子生物学研究
刘继红,男。1999年毕业于华中农业大学,获博士学位。2000年和2003年分别晋升为副教授和教授,2004年遴选为博士生导师。2003年11月-2005年11月和2007年9月-2008年2月受日本学术振兴会(JSPS)资助在日本国立果树研究所(筑波)从事博士后和访问学者研究。中国园艺学会亚热带南亚热带果树分会常务理事、湖北省植物生理学会理事、湖北省柑橘学会理事。获“华中农业大学第三届十大青年岗位能手称号(2002年)”、“第九届霍英东教育基金会青年教师奖二等奖(2004年)”、“第10届中国农学会青年科技奖”(2006年)、“第九届湖北省青年科技奖”(2009年)”,入选2006年度教育部新世纪优秀人才支持计划。获国家科技进步奖二等奖(第六完成人,2006)和湖北省自然科学奖二等奖(第四完成人,2001年)。目前主要从事果树逆境生理与分子生物学研究,先后主持有国家自然科学基金、教育部新世纪优秀人才支持计划项目、霍英东教育基金会优选选资助课题、教育部博士点基金、教育部科学技术研究重点项目、国际科学基金、湖北省自然科学基金(青年杰出人才基金、面上项目和重点项目)、教育部留学回国人员科研启动基金、武汉市晨光计划等项目。自2009年起,任《Acta Physiologiae Plantarum》、《Journal of Horticultural Science & Biotechnology》、《Canadian Journal of Plant Science》、《Plant Cell, Tissue and Organ Culture》associate editor和《Biotechnology & Biotechnological Equipment》编委会成员。
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刘继红, Ji-Hong Liu, , Kazuyoshi Nada, Chikako Honda, Hiroyasu Kitashiba, Xiao-Peng Wen, *, Xiao-Ming Pang and Takaya Moriguchi, †
Journal of Experimental Botany, Vol.57, No.11, pp. 2589-2599, 2006,-0001,():
-1年11月30日
To clarify the involvement of the arginine decarboxylase (ADC) pathway in the salt stress response, the polyamine titre, putrescine biosynthetic gene expression, and enzyme activities were investigated in apple [Malus sylvestris (L.) Mill. var. domestica (Borkh.) Mansf.] in vitro callus under salt stress, during recovery after stress, and when ADC was inhibited by Darginine, an inhibitor of ADC. Salt stress (200 mM NaCl) caused an increase in thiobarbituric acid-reactive substances (TBARS) and electrolyte leakage (EL) of the callus, which was accompanied by an increase in free putrescine content, during 7 d of treatment. Conjugated putrescine was also increased, but this increase was limited to the early stage of salt stress. Accumulation of putrescine was in accordance with induction of ADC activity and expression of the apple ADC gene (MdADC). When callus that had been treated with 200 mM NaCl was transferred to fresh medium with (successive stress) or without (recovery) NaCl, TBARS and EL were significantly reduced in the recovery treatment, indicating promotion of formation of new callus cells, compared with the successive stress treatment. Meanwhile, MdADC expression and ADC activity were also decreased in the callus undergoing recovery, whereas those of the callus under successive stress were increased. Ornithine decarboxylase (ODC) activity showed a pattern opposite to that of ADC in these conditions. D-Arginine treatment led to more serious growth impairment than no treatment under salt stress. In addition, accumulation of putrescine, induction of MdADC, and activation of ADC in D-arginine-treated callus were not comparable with those of the untreated callus. Exogenous addition of putrescine could alleviate salt stress in terms of fresh weight increase and EL. All of these findings indicated that the ADC pathway was tightly involved in the salt stress response. Accumulation of putrescine under salt stress, the possible physiological role of putrescine in alleviating stress damage, and involvement of MdADC and ADC in response to salt stress are discussed.
Arginine decarboxylase (, ADC), ,, electrolyte leakage,, Malus sylvestris var., domestica,, MdADC,, putrescine,, salt stress,, thiobarbituric acid-reactive substances.,
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刘继红, Qinghua Zhang, Jihong Liu*, Xiuxin Deng
Journal of Plant Physiology 163 (2006) 1185-1192,-0001,():
-1年11月30日
The effect of hydroxyurea (HU) and amiprophos-methyl (APM) on the synchronization of suspension cultures of Satsuma mandarin (Citrus unshiu) and micronucleation of the suspension cells sequentially treated with both, HU and APM, were investigated. When suspension cultures in early-log phase were treated with 4 or 10mM HU for 24h, the number of cells in the S-phase and the mitotic index (MI) increased significantly. Exposure of the early-log phase suspension culture to 32mM APM led to a marked increase in the MI 12 and 24h after treatment, while higher as well as lower concentrations (16, 24 and 48 mM) had no effect. Suspension cultures subjected to sequential treatment, e.g. pretreatment with 10mM HU for 24h followed by treatment with 32 mM APM for 24h, also showed a considerably increased MI. Furthermore, 61.5% of the protoplasts isolated from the sequentially treated suspension cells were micronucleated, whereas only 3.6% of the control protoplasts, isolated from untreated cells, showed micronucleation. Ultra-centrifugation of the micronucleated protoplasts generated microprotoplasts of different sizes, most of them below 5 mM in diameter, with 1 or few chromosomes. The potential application of microprotoplasts in citrus genetic improvement is discussed.
Amiprophos-methyl, Citrus unshiu, Hydroxyurea, Micronucleation, Microprotoplast, Synchronization
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刘继红, Shao-Hua Zeng Æ Chuan-Wu Chen Æ Liu Hong Æ Ji-Hong Liu Æ Xiu-Xin Deng
Plant Cell Tiss Organ Cult (2006) 87: 85-93,-0001,():
-1年11月30日
In the present paper attempts were made to induce chromosome doubling of ‘Meiwa’ kumquat (Fortunella crassifolia) protoplasts and 'Frost' navel orange (Citrus sinensis Osbeck) embryogenic callus via colchicine treatment. Colchicine decreased protoplast viability, delayed protoplast division and inhibited callus growth, indicating presence of toxicity to cells. Cell lines established from 'Meiwa' protoplasts treated with 0.01 and 0.1% colchicine for 8, 16 and 24 h at each concentration showed different responses when they were cultured on embryoid-induction medium. Flow cytometry (FCM) demonstrated that tetraploids were detected in cell lines and embryoids from all of the treatments, with the highest frequency being 19.23%. As for 'Frost', tetraploid cells were only detected when the callus was treated with 0.1% colchicine for either 4 or 8 days, from which plantlets were regenerated. FCM and chromosome counting confirmed them as true tetraploids. The diploid cells were more active in mitotic division during a 12-day culture and smaller in size than their tetraploid counterpart. Potential applications of the novel tetraploid germplasms obtained through in vitro chromosome doubling to citrus cultivar improvement are discussed.
Potential applications of the novel tetraploid germplasms obtained through in vitro chromosome doubling to citrus cultivar improvement are discussed.,
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