刘建柱
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- 姓名:刘建柱
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学科领域:
畜牧科学、动物医学
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刘建柱,兽医学博士,山东农业大学副教授,中国畜牧兽医学会家畜内科学分会常务理事,“2005年度国家优秀自费留学生奖学金”、“2008年国际针灸学会“青年科学家”奖”获得者,在2006年获得韩国忠南大学授予的临床兽医学博士学位(兽医学博士)。先后主持归国留学基金项目“猪附红细胞体特异性蛋白免疫原性研究”,博士后特别资助“猪附红细胞体ORF基因编码蛋白抗原性鉴定、表位识别及其免疫原性检测”,山东省青年科学家奖励基金“猪附红细胞体MSG1基因人工定点诱变及对其抗原性的影响”山东农业大学博士基金“眼针对动物疾病治疗效果的研究”,山东省博士后基金“猪附红细胞体MSG1基因的克隆、表达及特异性ELISA方法的建立”;参加了自然基金“外源体在禽白血病病毒诱导的免疫抑制中的作用”,科技部国际合作项目“动物源人畜共患病的监测”,山东省科技攻关项目“猪附红细胞体对猪免疫功能的影响及免疫调节剂的开发研究”等的研究。现已在Vaccine、Veterinary Microbiology、Veterinary Parasitology、Research in Veterinary science等期刊杂志上发表研究性论文40余篇,其中SCI收录论文14篇。
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725
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成果数
9
【期刊论文】Surface-displayed porcine epidemic diarrhea viral (PEDV) antigens on lactic acid bacteria
刘建柱, Xi-Lin Houa, d, Li-Yun Yub, ∗, Jianzhu Liuc, ∗∗, Gui-Hua Wangb
Vaccine (2007) 26, 24-31,-0001,():
-1年11月30日
In this report, for surface display of viral antigen on lactobacilli, we have developed a surface antigen display system using the poly- -glutamate synthetase A protein (pgsA) of Bacillus subtilis as an anchoring matrix. Recombinant fusion proteins comprised of pgsA and neucleocapsid protein of PEDV were stably expressed in Lactobacillus casei. Surface location of fusion protein was verified by ELISA, immunofluoresence microscopy. Oral and intranasal inoculations of recombinant L. casei into pregnant sow and mice resulted in high levels of serum immunoglobuline G (IgG) and mucosal IgA, as demonstrated by rnELISA(recombinant N protein ELISA) using recombinant N protein. Absorbance of IgG in pregnant sow sera highly increased duration of the experiment. More importantly, the level of IgA in colostrum were increased significantly higher than that of IgG. The IgG levels of the piglets were increased after suckling colostrum secreted from sows previously inoculated recombinant L. casei. These results indicate that mucosal immunization with recombinant L. casei expressing PEDV N protein (neucleoprotein of PEDV) on its surface elicited high levels of mucosal IgA and circulation IgG immune responses against the antigen N of PEDV.
Porcine epidemic diarrhea viral (, PEDV), , Lactic acid bacteria, N protein
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【期刊论文】Prevalence of canine giardiosis in South Korea
刘建柱, J. Liu a, b, S.E. Lee a, K.H. Song a, *
Research in Veterinary Science 84 (2008) 416-418,-0001,():
-1年11月30日
This study was carried out a survey for the presence of Giardia intestinalis infections in dogs using a Giardia ELISA kit (SNAP test, IDEXX Laboratories, Inc., Westbrook, ME). Four hundred and seventy-two dogs (190 males and 282 females) were examined for a G. intestinalis infection in four different provinces (Kyunggi, Chonnam, Kyungnam and Chungnam) and a Seoul city in South Korea. Slightly more male dogs (15.0%) than female (8.6%) dogs were affected, there was no significant difference between the two groups. The overall positive rates of G. intestinalis infection were 11.2%, 14.9% being found in the <2-year-old group, 8.7% in the 2-5 year-old group, and 2.1% in the >5-year-old group. X2 analysis revealed a significantly higher prevalence (14.9%, P<0.05) in the <2-year-old group than in the other two groups, and a significantly higher prevalence in symptomatic dogs (47.6%, P<0.001) in asymptomatic dogs (7.7%). This study is the first large-scale survey of canine G. intestinalis infections in South Korea, and is expected provide a useful reference for clinicians. Giardia-infected dogs should be treated for hygienic management as well as to prevent dog to human transmission.
Dog, ELISA, Giardia intestinalis, Pre, v, a, l, ence
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刘建柱, J. Liu a, K.H. Song a, *, S.E. Lee a, J.Y. Lee b, J.I. Lee c, M. Hayasaki d, M.J. You e, D.H. Kim a
Veterinary Parasitology 130 (2005) 125-129,-0001,():
-1年11月30日
The aim of this study was to carry out a serological and molecular survey for the presence of Dirofilaria immitis infection in stray cats using an ELISA kit and PCR assay. One hundred and fifty-five stray cats (77 females and 78 males) in Gyunggi province in South Korea, were used in this study. Four (2.6%) tested with the ELISA kit showed a positive reaction, and all positive samples by the ELISA kit showed a positive reaction by PCR analysis. No significant difference was observed between the male (2.6%) and female (2.6%) cat groups by ELISA kit. The positive rates for dirofilariosis were 2.8% in the 4–6-year-old group, and 18.2% in the >6-year-old group by ELISA kit. With regard to the age element, older cats showed a higher prevalence of D. immitis infection in this study. A statistical analysis revealed that significant difference was observed in >6-year-old group (p<0.01). In conclusion, D. immitis infection in stray cats was present in Gyunggi province, although its incidence was low. Therefore, heartworm treatment and/or prophylaxis for stray cats captured are required in this area.
Cat, Dirofilaria immitis, PCR, ELISA, Pre, v, a, l, ence
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刘建柱, Jianzhu Liu, * Kun-Ho Song, † Myung-Jo You, § Dong-Soo Son, ¶ Sung-Whan Cho‡ and Duck-Hwan Kim†
The American Journal of Chinese Medicine, Vol.35, No.2, 241-250,-0001,():
-1年11月30日
The potential recovery effect by oculo-acupuncture (OA) on ethylene glycol-induced acute renal injury in dogs was investigated. Acute renal damage was induced by ingestion of ethylene glycol in six mongrel dogs. The dogs were assigned to control (three dogs) and experimental (three dogs) groups. The control group did not receive any treatment, while the experimental group was treated with oculo-acupuncture at kidney/urinary bladder region plus zhong jiao region of the eyes after the induction of renal damage. Serum blood urea nitrogen (BUN), creatinine, sodium (Na), chloride (Cl), and potassium (K) were measured in both control and experimental groups. The blood RBC and Hb were also examined. The serum BUN and creatinine activities in the experimental group were lower than those in the control group, the serum Na and Cl had the irregular change in both groups, and the blood Hb in the control and experimental group showed decreasing tendency. Significant differences were observed on the 3rd and 7th day in BUN, 7th day in creatinine, 2nd day in Na and Cl, and 7th day in Hb when compared to the control group. Whereas, serum K concentration and RBC in the experimental group did not change significantly. The recovery findings of the renal injury were also observed in the experimental group histopathologically. In conclusion, OA therapy (kidney/urinary bladder region plus zhong jiao region) was effective for recovery of the renal injury induced by ethylene glycol in dogs.
Oculo-Acupuncture, Renal Injury, Ethylene Glycol, Dogs.,
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【期刊论文】Anti-Emetic Effect of Oculo-Acupuncture on Dogs with Xylazine Induced Vomiting
刘建柱, Jianzhu Liu, * Yoo-Teak Lee, ‡ Sang-Eun Lee, † Jung-Yeon Lee† and Duck-Hwan Kim‡
The American Journal of Chinese Medicine, Vol.35, No.3, 447-453,-0001,():
-1年11月30日
The present study was conducted in order to clarify the anti-emetic effect of oculo-acupuncture (OA) on dogs with xylazine-induced vomiting, and also to compare the anti-emetic effect of OA and body acupuncture (AP). Twelve dogs induced to vomit by xylazine were selected from total 29 mongrel dogs in preliminary experiment and were used as subjects in this study. This study was comprised of two experiments. In experiment 1, the anti-emetic effects of OA on dogs were examined in the stomach/spleen region (experimental group I), the zhongjiao region (experimental group II), and the stomach/spleen region plus the zhongjiao region (experimental group III) using 12 dogs induced to vomit for one week interval repeatedly. On the other hand, needle acupuncture (AP) (BL20 + BL21, experimental group A) and OA (stomach/spleen and zhong jiao regions) combined with needle AP (BL20+BL21) (experimental group B) were examined using 6 vomiting dogs, for one week interval repeatedly in experiment 2. As a result, the vomiting rates of experimental group I (50%, p<0.05), experimental group II (58.3%) and experimental group III (41.6%, p<0.01) were lower than that of control (100%), respectively in experiment 1. The vomiting rates of both experimental group A (50%, p<0.05) and experimental group B (50%, p<0.05) were lower than that of control (100%) in experiment 2. The starting vomiting time in experimental groups was similar to that of the control groups in experiment 1 and 2. This study demonstrated that OA had anti-emetic effects on dogs with xylazine-induced vomiting and OA in the stomach/spleen region plus the zhongjiao region was the most effective in anti-emesis among the experimental groups. In addition, body AP and OA combined with body AP had a similar anti-emetic effect on dogs with xylazine-induced vomiting.
Anti-Emetic, Oculo-Acupuncture, Dogs, Xylazine
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刘建柱, Jianzhu Liu, * Sung-Nam Cho, † Kun-Ho Song, † Duck-Hwan Kim, †, Myung-Cheol Kim‡ and Sung-Whan Cho§
The American Journal of Chinese Medicine, Vol.35, No.1, 53-61,-0001,():
-1年11月30日
We investigated the therapeutic effect of oculo-acupuncture on dogs induced with acute hepatic injury. Hepatic injury was induced by intraperitoneal injection with carbon tetrachloride (CCl4) in 8 mongrel dogs (4 females and 4 males, aged 2 to 4 years). The dogs were divided into the control group (4 dogs) and the experimental group (4 dogs). The experimental group was treated with oculo-acupuncture at the liver/gallbladder regions plus the zhong jiao region of the eye after the induction of hepatic injury. Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and gamma glutamyl transpeptidase (GGT) activities were measured in both control and experimental groups. The serum AST, ALT, and GGT activities in the experimental group were decreased as compared to those in the control group. The significant differences were detected on the third day (AST, p<0.05), second day (ALT, p<0.05) and third day (GGT, p<0.05) in the experimental group, respectively. Oculo-acupuncture alleviated acute liver damage induced by carbon tetrachloride in dogs was also confirmed by histopathological examination. We concluded that oculo-acupuncture at the liver/gallbladder regions plus the zhong jiao region was effective in the recovery of dogs from hepatic injury in a CCl4-induced model.
Oculo-Acupuncture, CCl4, Liver Injury, Dog.,
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【期刊论文】The Effects of Fu Zi on Changes in the Body Heat of Dogs
刘建柱, Tian-Tian Chen, Chunhua Qi, , Huijun Guo, Ziqiang Cheng, Dong Zhou, Haitao Liu, Jianzhu Liu*
J Acupunct Meridian Stud 2009; 2 (1): 71-74,-0001,():
-1年11月30日
The aim of the present study was to investigate the effects of Fu Zi on changes in the body heat of dogs. Twelve clinically healthy dogs were divided into two groups: the control group (six dogs) and the experimental group (six dogs). The control group was made to ingest normal saline mixed with canned meat, while the experimental group was made to ingest the Fu Zi solution mixed with canned meat. The infrared thermographic system was used to determine the level of body heat generated by these dogs. These areas include the dorsocranial (DCr), dorsocaudal (DCd), ventrocranial (VCr), and ventrocaudal (VCd) regions at pretreatment and were determined at 10, 20, 30, 50, 90, 120, 240, and 360 minutes after treatment for each of these areas. The results showed a tendency toward increased body heat until 30 minutes after ingestion of the Fu Zi powder mixed with canned meat. The significant differences in the changes of body heat were detected at 360 minutes in the DCd regions, 20 minutes in the VCr regions, and 30 minutes in the VCd regions between the experimental and control groups (p < 0.05). Based from our results, we find that Fu Zi can increase and maintain the dogs’ body heat for at least 6 hours.
Aconitum carmichaeli Debx, body heat, dog
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【期刊论文】The Therapeutic Effect of Ozone on Contagious Ecthyma in Korean Native Goats
刘建柱, Jianzhu Liu, In Heo*, Min-Sung Hong, Ji-Min Seo, Sung-Nam Jo, Jung-Yeon Lee**, Kun-Ho Song and Duck-Hwan Kim
J Vet Clin 23(l): 14-17 (2006),-0001,():
-1年11月30日
We investigated possible therapeutic approaches to treat contagious naturally occurring ecthyma in Korean native goats in the Gongju area of Korea. Contagious ecthyma was found in 130 of 150 Korean native goats studied. Sixteen goats, ranged from 2 months to 2.5 years old, were selected for further experiments. The goats were divided into a control group, an ozonated oil group, and an ozonated water group consisting of 5, 5, and 6 goats, respectively. The control animals were not treated during the experimental period. The ozonated oil group was treated with ozonated oil, made by bubbling 200 ppm ozone gas into the oil, daily for 2 weeks. The remaining group of animals were given ozonated drinking water (0.1 ppm) ad libitum for 2 weeks. The ozonated oil treated group (4/5, 80%) had the highest therapeutic rate. The ozonated water group (4/6, 66.7%) had the second highest therapeutic rate, followed by the control group (1/5, 20%). Based on these results, a secondary experiment was performed that included a total of 121 Korean native goats with contagious ecthyma. Ozonated oil was applied daily for 30 days. All of the lesions on the goats with contagious ecthyma were completely cured by application of the ozonated oil. We concluded that ozone therapy was an effective treatment for contagious ecthyma in goats
therapeutic effect,, ozone herapy,, contagious ecthyma,, goats.,
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刘建柱, Zhengui Yan, Jianzhu Liu*, Tiantian Chen, Ziqiang Cheng, Huijun Guo, Zhenyong Wang, Yong Wang
J Acupunct Meridian Stud 2008; 1 (2): 143-148,-0001,():
-1年11月30日
Sixteen shorthorn cows from Xiazhuang farm were admitted to the Veterinary Teaching Hospital at the College of Animal Science and Veterinary Medicine, Shandong Agricultural University for evaluation of poor appetite, listlessness, fever, tachycardia, tachypnea, lethargy, positive jugular venous pulse and anemia. Blood smear examination and polymerase chain reaction analysis in these cows revealed an infection with Mycoplasma wenyonii. The subjects were divided into two groups: control group (three cows) treated with intramuscular injection with imidocarb dipropionate (3 mg/kg/day for 2 days) and the experimental group (13 cows), treated with injection-acupuncture (Imidocarb Dipropionate, 1 mg/kg, once every 3 days for 6 days) at BL17, BL18, BL20, BL25, ST36, SP06 and CV04. At day 15, negative results were found using blood smear examination in all control and experimental groups.
acupuncture, cow, imidocarb dipropionate, Mycoplasma
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