徐昌杰
1.果实品质分子生理学; 2.果实采后分子生理学; 3.果树生物技术。
个性化签名
- 姓名:徐昌杰
- 目前身份:
- 担任导师情况:
- 学位:
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学术头衔:
博士生导师, 教育部“新世纪优秀人才支持计划”入选者
- 职称:-
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学科领域:
果树学
- 研究兴趣:1.果实品质分子生理学; 2.果实采后分子生理学; 3.果树生物技术。
徐昌杰,男,浙江台州人。博士,教授, 博士生导师,果树科学研究所所长助理。霍英东教育基金获得者,并入选教育部"新世纪优秀人才支持计划"和“浙江省新世纪151人才工程”(第二层次)。兼任中国园艺学会杨梅分会秘书长、中国园艺学会采后科学技术分会副秘书长、浙江省园艺学会副秘书长、浙江省柑桔产业协会副秘书长。
学历:1996.-1999.3 浙江大学园艺系 博士;1993-1996 浙江大学园艺系 硕士;1989-1993 华南农业大学生物系 学士。
工作简历:1999.03-至今 浙江大学任教。2001.06-2001.09 新西兰国家研究院园艺与食品研究所,短期工作访问。2004.12-2005.11 英国伦敦大学皇家霍洛威学院访问学者。
研究方向:1.果实品质分子生理学; 2.果实采后分子生理学; 3.果树生物技术。
近年主持的主要课题:国家自然科学基金 枇杷果实类胡萝卜素积累的分子生理机制 2009-2011 ;国家自然科学基金 柑桔果实类胡萝卜素合成关键基因鉴别及其表达特性研究 2007-2009 ;国家自然科学基金 红肉脐橙果实积累番茄红素的分子基础研究 2004-2006 ;国家自然科学基金 果实组织特异性启动子的分离及功能鉴定 2002-2004 ;国家科技支撑子课题 农产品储藏期间的冷害控制技术 2006-2010 ;教育部新世纪优秀人才支持计划 柑桔DXS基因特性及其在果实萜类物质合成中的作用 2008-2010 ;农业公益性行业科研专项经费项目子课题 柑桔模式化栽培及贮藏技术研究 2007-2010 ;浙江省自然科学基金重点项目 Rac GTPase基因对枇杷果实冷胁迫木质化的调控 2007-2009 ;浙江省科技厅农业重点项目 控制柑桔果实类胡萝卜素合成的关键基因及其功能调控 2005-2007 ;浙江省自然科学基金 柑桔成熟果实特异启动子的分离、鉴定与应用 2002-2004。
主要奖励:1. 果实采后品质变化及其调控的分子生理机制。教育部自然科学奖一等奖,2007年, 第二完成人;2. 柑橘果实品质形成与调控的分子生理。教育部自然科学奖二等奖,2008年, 第三完成人;3. 永嘉早香柚标准化生产关键技术研究及推广。浙江省科技进步奖三等奖,2006年, 第四完成人。
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【期刊论文】DNA quantiWcation using EvaGreen and a real-time PCR instrument
徐昌杰, Weijie Wang, Kunsong Chen, Changjie Xu¤
Analytical Biochemistry 356(2006)303-305,-0001,():
-1年11月30日
DNA quantiWcation is an important, frequently used technique, and inaccuracies can result in failures with ligation, restriction, polymerase chain reaction (PCR),1 ampli-Wed fragment length polymorphism (AFLP), Southern blotting, and other techniques. DNA is most commonly quantiWed using absorbance at 260nm, but because of the existence of many impurities, this can be an imprecise measurement and DNA levels can be more than 10 times overestimated in some cases [1]. QuantiWcation by agarose gel electrophoresis with a known amount of standard DNA [1,2] can provide more accurate data, but the procedures are complicated, the data often still are not accurate enough, and the technique is impractical for routine or high-throughput DNA quantiWcation [3]. Fluorescence spectroscopy using various DNA intercalating dyes is the most widely accepted technique for accurate DNA quanti-Wcation [4]. However, if the analysis is to be carried out with a Xuorescence spectrophotometer, a relatively large assay volume (e.g., 2ml) is required [5], and this is impractical for small DNA samples and expensive dyes. Fluorescence can also be measured with a smaller volume of DNA sample using other instruments such as Xuorescent microplate readers [6], microplate Xuorometers [7,8], and transilluminator-microplate-CCD camera systems [9], but the instruments might not be readily available in most molecular biology laboratories.
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【期刊论文】Carotenoids in White- and Red-Fleshed Loquat Fruits
徐昌杰, CHUN-HUA ZHOU, †, ‡ CHANG-JIE XU, § CHONG-DE SUN, † XIAN LI, † AND KUN-SONG CHEN*
Agric. Food Chem. 2007, 55, 7822-7830,-0001,():
-1年11月30日
Fruits of 23 loquat (Eriobotrya japonica Lindl.) cultivars, of which 11 were white-fleshed and 12 red-fleshed, were analyzed for color, carotenoid content, and vitamin A values. Color differences between two loquat groups were observed in the peel as well as in the flesh. β-Carotene and lutein were the major carotenoids in the peel, which accounted for about 60% of the total colored carotenoids in both red- and white-fleshed cultivars. β-Cryptoxanthin and, in some red-fleshed cultivars, β-carotene were the most abundant carotenoids in the flesh, and in total, they accounted for over half of the colored carotenoids. Neoxanthin, violaxanthin, luteoxanthin, 9-cis-violaxanthin, phytoene, phytofluene, andβ-carotene were also identified, while zeaxanthin, R-carotene, and lycopene were undetectable. Xanthophylls were highly esterified. On average, 1.3- and 10.8-fold higher levels of colored carotenoids were observed in the peel and flesh tissue of red-fleshed cultivars, respectively. The percentage of -carotene among colored carotenoids was higher in both the peel and the flesh of red-fleshed cultivars. Correlations between the levels of total colored carotenoids and the color indices were analyzed. The a* and the ratio of a*/b* were positively correlated with the total content of colored carotenoids, while L*, b*, and H°correlated negatively. Vitamin A values, as retinol equivalents (RE), of loquat flesh were 0.49 and 8.77µg/g DW (8.46 and 136.41µg/100g FW) on average for whiteand red-fleshed cultivars, respectively. The RE values for the red-fleshed fruits were higher than fruits such as mango, red watermelon, papaya, and orange as reported in the literature, suggesting that loquat is an excellent source of provitamin A.
Carotenoids, provitamin A, color, high-performance liquid chromatography, Eriobotrya japonica
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徐昌杰, Chong Caia, ChangJie Xua, Xian Lia, Ian Fergusona, b, KunSong Chena, *, a
Postharvest Biology and Technology 40(2006)163-169,-0001,():
-1年11月30日
Loquat (Eriobotrya japonica Lindl.) fruit are non-climacteric and have a short postharvest life. During postharvest ripening over 8d at 20℃, fruit firmness increased and showed a positive correlation with accumulation of lignin in the flesh. Among the enzymes associated with lignin synthesis, phenylalanine ammonia lyase (PAL) activity increased rapidly during the first 3d after harvest and then declined in the fruit flesh, while cinnamyl alcohol dehydrogenase (CAD) and peroxidase (POD) activities showed a persistent rise over the whole 8d. Accumulation of lignin in flesh tissue was also positively correlated to activities of CAD, guaiacol-POD (G-POD) and syringaldazine-POD (S-POD). Cellulose content in flesh tissue decreased and showed a significant negative correlation with lignin content. Where fruit ripening was enhanced by ethylene treatment, or retarded by low temperature or use of 1-methylcyclopropene (1-MCP), the inhibitor of ethylene reception, firmness, lignification and the enzyme activities were consistently enhanced or retarded accordingly. Our results suggest that increase in firmness of loquat fruit during ripening is a consequence of tissue lignification, a process associated with increases in PAL, CAD and POD activities, and might involve a coordinated regulation of lignin biosynthesis and cellulose hydrolysis.
Cellulose, Cinnamyl alcohol dehydrogenase, Ethylene, Lignification, Lignin, Loquat, 1-MCP, Phenylalanine ammonia lyase, Peroxidase, Postharvest, Senescence
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【期刊论文】Low temperature conditioning reduces postharvest chilling injury in loquat fruit
徐昌杰, Chong Caia, ChangJie Xua, LanLan Shana, Xian Lia, ChunHua Zhoua, WangShu Zhanga, Ian Fergusona, b, KunSong Chena*
Postharvest Biology and Technology 41(2006)252-259,-0001,():
-1年11月30日
Chilling injury occurs in loquat (Eriobotrya japonica Lindl. cv. Luoyangqing) fruit when they are stored at temperatures lower than 5℃. In attempts to reduce this chilling injury, the effect of low temperature conditioning (LTC) was examined. Loquat fruit were conditioned at 5℃ for 6 days before 0℃ storage for up to 54 days. Control fruit stored at 0℃ exhibited severe symptoms of lignification and tissue browning, and a decrease in percentage juice. LTC treatment significantly reduced these chilling injury symptoms, and doubled storage life. In terms of acceptability (tissue browning, internal browning (IB) index <0.4; fruit decay, <10%; flesh firmness, <6.0 N; percentage juice, >60%), fruit could only be stored for 40 days at 0℃ with a 3 days shelf life at 20℃, while LTC fruit could be stored for 60 days at 0℃ with a similar shelf life. Similarly, LTC fruit could be stored for 40 days with a 5 days shelf life at 20℃, while fruit could be only stored for 20 days at 0℃with a 5 days shelf life. Our results confirm that LTC can effectively alleviate postharvest chilling injury of loquat fruit and may provide longer storage life with acceptable external and internal quality.
Loquat, Chilling injury, Lignification, Browning, Fruit quality
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