陈忠周
博士研究生 教授
中国农业大学 生物学院
主要研究方向为蛋白质晶体学、结构生物学、植物生理学、病毒学、肿瘤学。
个性化签名
- 姓名:陈忠周
- 目前身份:在职研究人员
- 担任导师情况:
- 学位:
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学术头衔:
博士生导师, 教育部“新世纪优秀人才支持计划”入选者
- 职称:高级-教授
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学科领域:
物理海洋学
- 研究兴趣:主要研究方向为蛋白质晶体学、结构生物学、植物生理学、病毒学、肿瘤学。
陈忠周,中国农业大学生物学院教授、博士生导师。
1997年毕业于清华大学,取得物理化学及仪器分析专业和企业管理专业理学双学士学位;2002年毕业于清华大学,取得化学生物学专业理学博士学位;2002年—2007年在美国国立犹太人医学研究中心免疫系任助理研究员;2007年至今在中国农业大学生物学院任教。
主要研究方向为蛋白质晶体学、结构生物学、植物生理学、病毒学、肿瘤学。主持的项目主要有973、自然科学基金面上及优青、教育部新世纪优秀人才、教育部博士点基金等。迄今发表的较高质量的SCI论文已有30多篇, 以第一作者或通讯作者在Cell, Genes & Development, Nature Comm., PNAS, Cell Research, Nucleic Acids Research, JBC, Structure, Acta Crystallogr D Biol Crystallogr.等和化学著名的期刊JOC, JPCA等发表多篇高质量论文。
兼任美国晶体学协会会员中国晶体学学会终身会员、中国生物物理学会会员。
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15
【期刊论文】Function and Regulation of SPLUNC1 Protein in Mycoplasma Infection and Allergic Inflammation1
陈忠周, Hong Wei Chu, * Jyoti Thaikoottathil, * John G. Rino, * Gongyi Zhang, † Qun Wu, * Taylor Moss, * Yosef Refaeli, ‡ Russell Bowler, * Sally E. Wenzel, § Zhongzhou Chen, † Jeffrey Zdunek, * Rachel Breed, * Ryan Young, ‡ Erin Allaire, * and Richard J. Martin*
The Journal of Immunology, 2007, 179: 3995-4002.,-0001,():
-1年11月30日
Respiratory infections, including Mycoplasma pneumoniae (Mp), contribute to asthma pathobiology. To date, the mechanisms underlying the increased susceptibility of asthmatics to airway Mp infection remain unclear. Short palate, lung, and nasal epithelium clone 1 (SPLUNC1) protein is a recently described large airway epithelial cell-derived molecule that was predicted to exert host defense activities. However, SPLUNC1 function and regulation in an infectious or allergic milieu are still unknown. We determined host defense and anti-inflammatory functions of SPLUNC1 protein in Mp infection and the regulation of SPLUNC1 by Mp and allergic inflammation (e.g., IL-13). SPLUNC1 function was examined in Mp or human airway epithelial cell cultures by using SPLUNC1 recombinant protein, overexpression and RNA interference. Human and mouse bronchial epithelial SPLUNC1 was examined using immunostaining, Western blotting, ELISA, laser capture microdissection, and real-time PCR. Mouse models of Mp infection and allergic inflammation and air-liquid interface cultures of normal human primary bronchial epithelial cells were used to study SPLUNC1 regulation by Mp and IL-13. We found that: 1) SPLUNC1 protein decreased Mp levels and inhibited epithelial IL-8 production induced by Mp-derived lipoproteins; 2) normal human and mouse large airway epithelial cells expressed high levels of SPLUNC1; and 3) although Mp infection increased SPLUNC1, IL-13 significantly decreased SPLUNC1 expression and Mp clearance. Our results suggest that SPLUNC1 serves as a novel host defense protein against Mp and that an allergic setting markedly reduces SPLUNC1 expression, which may in part contribute to the persistent nature of bacterial infections in allergic airways.
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【期刊论文】Structural Insights into Histone Demethylation by JMJD2 Family Members
陈忠周, Zhongzhou Chen, , Jianye Zang, Johnathan Whetstine, Xia Hong, Foteini Davrazou, Tatiana G. Kutateladze, Michael Simpson, Qilong Mao, Cheol-Ho Pan, Shaodong Dai, James Hagman, Kirk Hansen, Yang Shi, and Gongyi Zhang, *
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-1年11月30日
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【期刊论文】Reversal of Histone Lysine Trimethylation by the JMJD2 Family of Histone Demethylases
陈忠周, Johnathan R. Whetstine, Amanda Nottke, Fei Lan, Maite Huarte, Sarit Smolikov, Zhongzhou Chen, Eric Spooner, En Li, Gongyi Zhang, Monica Colaiacovo, and Yang Shi , *
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-1年11月30日
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【期刊论文】The use of crude lipase in deprotection of C-terminal protecting groups
陈忠周, Zhong-Zhou Chen, Yan-Mei Li∗, Xi Peng, Fu-Rong Huang, Yu-Fen Zhao
Journal of Molecular Catalysis B: Enzymatic 18(2002)243-249,-0001,():
-1年11月30日
A crude lipase, Newlase F, was used to remove C-terminal protecting groups from dipeptide esters. Hydrolysis of dipeptide n-heptyl esters with Newlase F was conducted in aqueous media containing acetonitrile. The optimum pH and temperature of lipase in Newlase F were 7.0 and 30 ◦C, respectively. Low level acetonitrile promoted the hydrolysis of dipeptide n-heptyl esters, while high level acetonitrile inhibited the hydrolysis. However, the protease activity in Newlase F was significantly inhibited by acetonitrile. Lipase in Newlase F worked better in a medium containing water-miscible organic solvents than in water-immiscible ones. N-terminal protecting groups were not affected by the protease in the crude enzyme. It was found that the protease in Newlase F did not hydrolyze amide bond with hydrophilic amino acids on either side under these conditions (pH 7.0, room temperature). Newlase F may consequently be used widely in the synthesis of peptide conjugates. The crude enzyme was immobilized on SBA-15 mesoporous molecular sieve. The lipase activity of immobilized preparation was more active on hydrolysis of C-terminal protecting groups and stable than the free enzyme. The immobilization also reduced the protease activity.
Newlase F, Dipeptide heptyl esters, Lipase, Protease
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【期刊论文】Differentiation of α-COOH from γ-COOH in glutamic acid by N-phosphorylation
陈忠周, Zhong-Zhou Chen, Bo Tan, Yan-Mei Li*, Yi Chen, Chang-Mei Cheng, Yu-Fen Zhao
Journal of Molecular Sturcture (Theocbem) 574(2001)163-175,-0001,():
-1年11月30日
The biomimic reactions of N phosphoryl amino acids are very important in the study of many biochemical processes, it was found that the α-COOH group, and not the γ-COOH, was involved in the ester exchange on phosphorus experimentally. The reactions, had been proposed to be related to intramolecular penta coordinate phosphoric carboxylic mixed anhydiides. N Dimethylphosphoryl glutamic acid (DMP Glu) was selected as model compound to study the reactivity difference between the α-COOH and γ-COOH group. From MNDO calculations, the energy of the penta coordinate phosphoric intermediates contain ing five membered ring from a COOH was 40.8 ld/mol lower than that of the seven membered one from γ-COOH. This resnlt was in agreement with those from HF/6-31G** and B3LYP/6 31G** calculations. Theoretical tln-ee dimensional potential energy snrface of frothing the intermdiates predicted that the transition states 4 and 5 involving α-COOH or γ-COOH gronp had energy baniers of △E=196.1 and 216.9 kJ/mol, respectively. So the α-COOH conld be differentiated from γ-COOH intramolecnlarly in glutantic acid by N phosphorylation.
Penta-cooidinate phosphoiic intermediates, MNDO, Glutamic acid, α-Carboxyhc gioup, γ-Carboxyhc gioup
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【期刊论文】The Self-catalytic Esterification Reaction of O-Phosphoryl Serine Derivative
陈忠周, Jin Tang DU, Yan Mei LI∗, Zhong Zhou CHEN, Shi Zhong LUO, Yu Fen ZHAO
Chinese Chemical Letters Vol.16, No.7, pp 856-858, 2005,-0001,():
-1年11月30日
O-Phosphoryl serine derivative can perform self-catalytic esterification reaction in the mixture of CH3OH and CHCl3 at the room temperature. The phosphoryl group participation was the key step of the esterification. This type of reactions were proposed through an intermediate of mixed phosphoric-carboxylic anhydride that might provide a clue to the function of the phosphoryl group in the phosphorylated enzymes and in the prebiotic synthesis of protein.
O-Phosphoryl serine, self esterification, phosphoryl group participation.,
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陈忠周, 杜进堂, 李艳梅, 谭波, 江洋, 钟儒刚, 赵玉芬
高等学校化学学报,2005,26,540~545,-0001,():
-1年11月30日
采用量子化学计算研究了磷酰化氨基酸的反应性质, 包括N-磷酰氨基酸成肽反应、成酯反应、磷上酯交换反应和磷上的N→O 迁移反应的机理以及计算模型和计算方法的选择, 并从理论上解释了自然界选择α-氨基酸而不是β, γ-氨基酸的实验事实.
N-磷酰氨基酸, 五配位磷中间体, 量子化学计算, 从头算, 密度泛函
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陈忠周, Bo Tan a, *, Matthew C. Lee b, Meng Cui c, Tong Liu c, Zhong-Zhou Chen a, Yan-Mei Li a, Yong Jua, Yu-Fen Zhao a, Kaixian Chen c, Hualiang Jiang c
Journal of Molecular Structure (Theochem) 672 (2004) 51-60,-0001,():
-1年11月30日
N-phosphoryl amino acids (PAA) are a unique chemical species with many chemical reactivities and properties, including the ability to self-assemble into oligopeptides. Because of their chemical characteristics, they have been proposed as the common origin for both nucleic acids and proteins. In this paper, we discussed six intramolecular mixed carboxylic-phosphoric anhydrides (IMCPAs) as possible intermediates for the prebiotic synthesis of biopolymers from PAA. We also investigated the role of water molecules in the formation of IMCPA using density functional theory B3LYP quantum mechanical calculations at the 6-31G** and 6-311 G** levels. Our results showed that the energy barrier of IMCPA formation was reduced from 19.5 kcal mol21 to 9.9 kcal mol21 when water participation and solvent effects were considered. Thus, the direct participation of one water molecule and the bulk solvent effects both play important roles in the formation of IMCPA.
Origins of life, N-phosphoryl amino acids, Intramolecular mixed carboxylic-phosphoric anhydride
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陈忠周, 李艳梅, 赵刚, 赵玉芬
有机化学,2001,21(4):273-277,-0001,():
-1年11月30日
系统地介绍和评述了近年来氨基、羧基、巯基和羟基等基团的酶催化脱保护方法的进展。由于酶催化脱保护方法具有反应条件温和(中性,水溶液)、选择性和专一性高、副反应少等优点,故在多肽缀合物、糖等敏感多官能团化合物的研究中有很广阔的应用前景。
酶催化, 脱保护, 氨基, 羧基, 羟基, 多肽缀合物
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【期刊论文】Hydrolysis of Dipeptide Heptyl Esters with Newlase F
陈忠周, Zhong Zhou CHEN, Yan Mei LI*, Xiu Fen KOU, Xue LI, Fu Rong HUANG, Hai Yan WANG, Ming Ming MA, Yu Fen ZHAO
Chinese Chemical Letters Vol.12, No.8, pp 693-696, 2001,-0001,():
-1年11月30日
Newlase F is a rude enzyme which contains triacylglycerol lipase and acid protease. Hydrolysis of dipeptide heptyl esters with Newlase F was studied in phosphate buffer-organic solvent by HPLC. When the Newlase F's level reached 5 mg/mL under mild condition (pH 7.0, 30℃), the lipase had the highest activity. The reaction was also affected greatly by organic solvents and their concentrations. It is found that protease in Newlase F does not hydrolyze amide bond under this condition (pH 7.0, r.t.).
Newlase F, dipeptide heptyl esters, HPLC.,
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