金危危
博士 教授
中国农业大学 农学与生物技术学院植物遗传育种学系
一直从事植物基因组学研究。
个性化签名
- 姓名:金危危
- 目前身份:在职研究人员
- 担任导师情况:
- 学位:博士
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学术头衔:
博士生导师, 教育部“新世纪优秀人才支持计划”入选者
- 职称:高级-教授
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学科领域:
作物育种学与良种繁育学
- 研究兴趣:一直从事植物基因组学研究。
金危危,男,1971年8月生,籍贯湖南沅陵。1993年7月毕业于湖南农业大学,获学士学位。2001年8月毕业于武汉大学,获博士学位。2002年2月~2004月12月:美国威斯康星大学 博士后(Research Associate)。2005年1月~2006年6月:美国威斯康星大学 助理研究员(Assistant Scientist)。2006年7月~ 中国农业大学 教授、博士生导师。近年来一直从事植物基因组学研究。主要包括:玉米等作物染色体着丝粒的结构和功能研究;玉米近缘种(薏苡、大刍草等)的基因组学研究;玉米单倍体诱导机理的研究;玉米减数分裂相关基因及其他重要功能基因的克隆; 其他植物分子细胞遗传学研究。主持或参加国家自然科学基金、863项目、973项目、农业部重大专项、948项目等。担任《Chromosome Research》、《作物学报》 、《The Crop Journal》杂志编委、亚洲动植物染色体研讨会国际执委、《中国农业大学学报》编委会副主任委员。
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1956
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0
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494
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成果数
7
金危危, Yonghua Han & Guixiang Wang & Zhao Liu &Jinhua Liu & Wei Yue & Rentao Song & Xueyong Zhang &Weiwei Jin
Chromosoma (2010) 119: 89-98,-0001,():
-1年11月30日
Knowledge about the composition and structure ofcentromeres is critical for understanding how centromeresperform their functional roles. Here, we report the sequencesof one centromere-associated bacterial artificial chromosomeclone from a Coix lacryma-jobi library. Two Ty3/gypsy-classretrotransposons, centromeric retrotransposon of C. lacrymajobi(CRC) and peri-centromeric retrotransposon of C.lacryma-jobi, and a (peri)centromere-specific tandem repeatwith a unit length of 153 bp were identified. The CRC ishighly homologous to centromere-specific retrotransposonsreported in grass species. An 80-bp DNA region in the153-bp satellite repeat was found to be conserved tocentromeric satellite repeats from maize, rice, and pearlmillet. Fluorescence in situ hybridization showed that thethree repetitive sequences were located in (peri-)centromericregions of both C. lacryma-jobi and Coix aquatica.However, the 153-bp satellite repeat was only detected on20 out of the 30 chromosomes in C. aquatica. Immunostainingwith an antibody against rice CENH3 indicates that the153-bp satellite repeat and CRC might be both the majorcomponents for functional centromeres, but not all the153-bp satellite repeats or CRC sequences are associatedwith CENH3. The evolution of centromeric repeats of C.lacryma-jobi during the polyploidization was discussed.
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金危危, Yonghua Hana, b, , Zhonghua Zhangc, Chunxia Liua, Jinhua Liua, Sanwen Huangc, Jiming Jiangd, and Weiwei Jina
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-1年11月30日
The centromere of an eukaryotic chromosome can move to a newposition during evolution, which may result in a major alterationof the chromosome morphology and karyotype. This centromererepositioning phenomenon has been extensively documented inmammalian species and was implicated to play an important rolein mammalian genome evolution. Here we report a centromererepositioning event in plant species. Comparative fluorescence insitu hybridization mapping using common sets of fosmid clonesbetween two pairs of cucumber (Cucumis sativus L.) and melon(Cucumis melo L.) chromosomes revealed changes in centromerepositions during evolution. Pachytene chromosome analysis revealedthat the current centromeres of all four cucumber andmelon chromosomes are associated with distinct pericentromericheterochromatin. Interestingly, inactivation of a centromere in theoriginal centromeric region was associated with a loss or erosionof its affixed pericentromeric heterochromatin. Thus, both centromereactivation and inactivation in cucurbit species were associatedwith a gain/loss of a large amount of pericentromeric heterochromatin.
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【期刊论文】An Integrated Genetic and Cytogenetic Map of theCucumber Genome
金危危, Yi Ren., Zhonghua Zhang., Jinhua Liu., Jack E. Staub, Yonghua Han, Zhouchao Cheng, XuefengLi, Jingyuan Lu, Han Miao, Houxiang Kang, Bingyan Xie, Xingfang Gu, Xiaowu Wang, YongchenDu, Weiwei Jin*, Sanwen Huang*
,-0001,():
-1年11月30日
The Cucurbitaceae includes important crops such as cucumber, melon, watermelon, squash and pumpkin. However, fewgenetic and genomic resources are available for plant improvement. Some cucurbit species such as cucumber have anarrow genetic base, which impedes construction of saturated molecular linkage maps. We report herein the developmentof highly polymorphic simple sequence repeat (SSR) markers originated from whole genome shotgun sequencing and thesubsequent construction of a high-density genetic linkage map. This map includes 995 SSRs in seven linkage groups whichspans in total 573 cM, and defines, 680 recombination breakpoints with an average of 0.58 cM between two markers.These linkage groups were then assigned to seven corresponding chromosomes using fluorescent in situ hybridization(FISH). FISH assays also revealed a chromosomal inversion between Cucumis subspecies [C. sativus var. sativus L. and var.hardwickii (R.) Alef], which resulted in marker clustering on the genetic map. A quarter of the mapped markers showedrelatively high polymorphism levels among 11 inbred lines of cucumber. Among the 995 markers, 49%, 26% and 22% wereconserved in melon, watermelon and pumpkin, respectively. This map will facilitate whole genome sequencing, positionalcloning, and molecular breeding in cucumber, and enable the integration of knowledge of gene and trait in cucurbits.
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【期刊论文】Molecular and Functional Dissection of the Maize BChromosome Centromere
金危危, Weiwei Jin, a Jonathan C. Lamb, b Juan M. Vega, b R. Kelly Dawe, c James A. Birchler, b, and Jiming Jianga,
,-0001,():
-1年11月30日
The centromere of the maize (Zea mays) B chromosome contains several megabases of a B-specific repeat (ZmBs), a156-bp satellite repeat (CentC), and centromere-specific retrotransposons (CRM elements). Here, we demonstrate that onlya small fraction of the ZmBs repeats interacts with CENH3, the histone H3 variant specific to centromeres. CentC, whichmarks the CENH3-associated chromatin in maize A centromeres, is restricted to an; 700-kb domain within the largercontext of the ZmBs repeats. The breakpoints of five B centromere misdivision derivatives are mapped within this domain.In addition, the fraction of this domain remaining after misdivision correlates well with the quantity of CENH3 on thecentromere. Thus, the functional boundaries of the B centromere are mapped to a relatively small CentC-and CRM-richregion that is embedded within multimegabase arrays of the ZmBs repeat. Our results demonstrate that the amount ofCENH3 at the B centromere can be varied, but with decreasing amounts, the function of the centromere becomes impaired.
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金危危, Huihuang Yan, a Weiwei Jin, a Kiyotaka Nagaki, a Shulan Tian, b Shu Ouyang, c C. Robin Buell, cPaul B. Talbert, d Steven Henikoff, d and Jiming Jianga,
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-1年11月30日
Centromeres are sites of spindle attachment for chromosome segregation. During meiosis, recombination is absent atcentromeres and surrounding regions. To understand the molecular basis for recombination suppression, we havecomprehensively annotated the 3.5-Mb region that spans a fully sequenced rice centromere. Although transcriptionalanalysis showed that the 750-kb CENH3-containing core is relatively deficient in genes, the recombination-free regiondiffers little in gene density from flanking regions that recombine. Likewise, the density of transposable elements is similarbetween the recombination-free region and flanking regions. We also measured levels of histone H4 acetylation and histoneH3 methylation at 176 genes within the 3.5-Mb span. Active genes showed enrichment of H4 acetylation and H3K4dimethylation as expected, including genes within the core. Our inability to detect sequence or histone modificationfeatures that distinguish recombination-free regions from flanking regions that recombine suggest that recombinationsuppression is an epigenetic feature of centromeres maintained by the assembly of CENH3-containing nucleosomes withinthe core. CENH3-containing centrochromatin does not appear to be distinguished by a unique combination of H3 and H4modifications. Rather, the varied distribution of histone modifications might reflect the composition and abundance ofsequence elements that inhabit centromeric DNA.
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【期刊论文】Maize Centromeres: Organization and Functional Adaptation in the Genetic Background of Oat
金危危, Weiwei Jin, a Juliana R. Melo, b Kiyotaka Nagaki, a Paul B. Talbert, c Steven Henikoff, c R. Kelly Dawe, b and Jiming Jianga,
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-1年11月30日
Centromeric DNA sequences in multicellular eukaryotes are often highly repetitive and are not unique to a specific centromere or to centromeres at all. Thus, it is a major challenge to study the fine structure of individual plant centromeres. We used a DNA fiber-fluorescence in situ hybridization approach to study individual maize (Zea mays) centromeres using oat (Avena sativa)-maize chromosome addition lines. The maize centromere-specific satellite repeat CentC in the addition lines allowed us to delineate the size and organization of centromeric DNA of individual maize chromosomes. We demonstrate that the cores of maize centromeres contain mainly CentC arrays and clusters of a centromere-specific retrotransposon, CRM. CentC and CRM sequences are highly intermingled. The amount of CentC/CRM sequence varies from; 300 to >2800 kb among different centromeres. The association of CentC and CRM with centromeric histone H3 (CENH3) was visualized by a sequential detection procedure on stretched centromeres. The analysis revealed that CENH3 is always associated with CentC and CRM but that not all CentC or CRM sequences are associated with CENH3. We further demonstrate that in the chromosomal addition lines in which two CenH3 genes were present, one from oat and one from maize, the oat CENH3 was consistently incorporated by the maize centromeres.
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