孙东晓
个性化签名
- 姓名:孙东晓
- 目前身份:
- 担任导师情况:
- 学位:
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学术头衔:
博士生导师, 教育部“新世纪优秀人才支持计划”入选者
- 职称:-
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学科领域:
家畜育种学
- 研究兴趣:
孙东晓,女,博士,1972.4.9出生
教育经历
2005.1-2006.10 访问学者,University of Virginia(美国),人类遗传学专业
1996.9-1999.7 博士,中国农业大学,动物遗传育种与繁殖专业
1993.9-1996.7 硕士,中国农业科学院研究生院,动物遗传育种与繁殖专业
1989.9-1993.7 学士,河北农业大学,畜牧专业
项目(课题)名称
项目来源 起止时间 科研经费 本人承担工作
奶牛分子育种技术研究 十一五科技支撑计划 2006-2010 70万 主持人
奶牛产奶性状主效基因分离和鉴定 863 2007-2010 70万 主持人
奶牛6号染色体产奶性状精细定位 新世纪优秀人才支持计划 2007-2010 50万 主持人
鸡DNA甲基化对生长性状基因表达的调控机制 国家自然科学基金 2003-2005 20万 主持人
猪、鸡重要经济性状的分子改良 973 2006-2010 400万 学术骨干
农业动物杂种优势遗传机理研究 973 2000-2005 280万 学术骨干
奶牛良种快速繁育关键技术研究与产业开发 十五国家重大科技专项 2002-2005 900万 学术骨干
奶牛群体遗传改良关键技术引进与中国优秀奶牛种群的扩繁 948 2006-2010 100万/年 学术骨干
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主页访问
1496
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关注数
0
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成果阅读
346
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成果数
6
【期刊论文】Regulation by Nicotine of Gpr51 and Ntrk2 Expression in Various Rat Brain Regions
孙东晓, Dongxiao Sun, , Weihua Huang, Yoon Y Hwang, Yuan Zhang, Qin Zhang and Ming D Li*
Neuropsychopharmacology (2007) 32, 110-116,-0001,():
-1年11月30日
Our previous genetic studies demonstrated that variants of the g-Aminobutyric acid B receptor subunit 2 (GPR51) and neurotrophic tyrosine kinase receptor type 2 (NTRK2) genes are significantly associated with nicotine dependence (ND) in smokers. However, whether such genetic associations lead to changes in the expression of the two genes in response to nicotine remains undetermined. In this study, we investigated the regulatory effect of nicotine on the expression of Gpr51 and Ntrk2 in seven rat brain regions during the administration of nicotine in a daily dose of 3.15 mg/kg for 7 days. With quantitative real-time RT-PCR, we found that nicotine increased the mRNA of Gpr51 by 70, 78, and 32% in the amygdala, striatum, and prefrontal cortex (PFC), respectively, but decreased by 54% in the nucleus accumbens (NA). The Gpr51 protein was upregulated by nicotine in the amygdala (26%), striatum (73%), PFC (28%), and medial basal hypothalamus (MBH; 19%) but downregulated in the NA (72%). Similarly, the mRNA level of Ntrk2 was enhanced by nicotine in the striatum (86%) and PFC (38%), but decreased in the NA (46%) and ventral tegmental area (VTA; 49%). A significant change in protein expression was also obtained for Ntrk2 in the PFC (24%), MBH (33%), NA (33%), and VTA (70%). Interestingly, these two genes showed a closely coordinated expression pattern in response to nicotine in most of the brain regions examined. In summary, our results demonstrate that the expression of Gpr51 and Ntrk2 is significantly regulated by nicotine at both the mRNA and protein levels in various brain regions, which provides further evidence that these two genes are involved in the etiology of ND, as reported in our previous genetic association studies in humans.
nicotine, rat brain, Gpr51, Ntrk2, expression
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孙东晓, MD Li, D Sun, , X-Y Lou, J Beuten, TJ Payne and JZ Ma
Molecular Psychiatry (2006), 1-12,-0001,():
-1年11月30日
Our previous linkage study demonstrated that the 9q22-q23 chromosome region showed a 'suggestive' linkage to nicotine dependence (ND) in the Framingham Heart Study population. In this study, we provide further evidence for the linkage of this region to ND in an independent sample. Within this region, the gene encoding Src homology 2 domain-containing transforming protein C3 (SHC3) represents a plausible candidate for association with ND, assessed by smoking quantity (SQ), the Heaviness of Smoking Index (HSI) and the Fagerstrom Test for ND (FTND). We utilized 11 single-nucleotide polymorphisms within SHC3 to examine the association with ND in 602 nuclear families of either African-American (AA) or European-American (EA) origin. Individual SNP-based analysis indicated three SNPs for AAs and one for EAs were significantly associated with at least one ND measure. Haplotype analysis revealed that the haplotypes A-C-T-A-T-A of rs12519-rs3750399-rs4877042–rs2297313-rs1547696-rs1331188, with a frequency of 27.8 and 17.6%, and C-T-A-G-T of rs3750399-rs4877042- rs2297313-rs3818668-rs1547696, at a frequency of 44.7 and 30.6% in the AA and Combined samples, respectively, were significantly inversely associated with the ND measures. In the EA sample, another haplotype with a frequency of 10.6%, A-G-T-G of rs1331188–rs1556384-rs4534195–rs1411836, showed a significant inverse association with ND measures. These associations remained significant after Bonferroni correction. We further demonstrated the SHC3 contributed 40.1-59.2% (depending on the ND measures) of the linkage signals detected on chromosome 9. As further support, we found that nicotine administered through infusion increased the Shc3 mRNA level by 60% in the rat striatum, and decreased it by 22% in the nucleus accumbens (NA). At the protein level, Shc3 was decreased by 38.0% in the NA and showed no change in the striatum. Together, these findings strongly implicate SHC3 in the etiology of ND, which represents an important biological candidate for further investigation. Molecular Psychiatry advance online publication, 19 December 2006; doi:10.1038/sj.mp.4001933
nicotine, linkage analysis, tobacco dependence, rat brain, SHC3, expression
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孙东晓, 徐青, 张沅*
科学通报,2005,50(17):1874~1878,-0001,():
-1年11月30日
以2个品种鸡亲本及其F1代基因组为实验材料,使用荧光标记替代甲基敏感扩增片段多态性(methylation sensitive amplified polymorphism, MSAP)方法中的同位素杨,优化了实验条件,建立了荧光标记的甲基敏感扩增片段多态性方法(fluorescent labeled methylation sensitive amplified polymorphism, F-MASP)。结果显示,鸡个体基因组的甲基化模式分为3种,甲基化片段约占40%;F1代与亲本比较,F1代甲基化多态模式约有95%来自亲本,变异的甲基化位点约5%,虽然变异的甲基化点数量少但种类多,共发现14种甲基化程度减弱型,12种甲基化程度增强型,结果表明,F-MSAP是一种有效地检测鸡基因组甲基化的方法,可以用于其他真核生物尤其是基因组复杂、甲基化多态性丰富的高等动物植物基因组甲基化研究。
荧光标记, 鸡, 基因组, 甲基化
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【期刊论文】蒙古绵羊和哈萨克绵羊MHC-DRB3基因外显子2的多态性
孙东晓, 张沅, , 李宁
遗传学报,2003,30(8):761~765,-0001,():
-1年11月30日
采用PCR-RFLP方法对蒙古绵羊和哈萨克绵羊MHC-DRB3基因第2外显子285bp的扩增产物进行多态性分析,共检测到,17种基因型,由A、B、C、D、E、F和H共7个复等位基因控制。通过酶切图谱分析表明,蒙古绵羊和哈萨克绵羊的MHC-DRB3基因第2外显子的第154、168和220位的碱基表现出多态性。统计分析表明,MHC-DRB3基因的部分基因型频率和等位基因频率在两个群体之间差异显著或极显著(P<0.10、P<0.05或P<0.01)。X2适合性检验结果表明,蒙古绵羊和哈萨克绵羊的MHC-DRB3基因第2外显子的HaeIII酶切位点均未达到Hardy-Weinberg平衡状态(P<0.01)。
蒙古绵羊, 哈萨克绵羊, MHC-DRB3, PCR-RFLP
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【期刊论文】蒙古山羊和哈萨克山羊GOL-DRB3基因的HaeIII酶切多态性分析
孙东晓, 孙东晓 张沅, 李宁
遗传 HEREDITAS(Beijing) 26(1): 55-58, 2004,-0001,():
-1年11月30日
采用限制性内切核酸酶HaeIII对蒙古山羊和哈萨克山羊GOLA-DRB3基因外显子2的285bp扩增产物进行了PCR-RFLP多态性分析,共检测到17种基因型,由A、B、C、D、E、F和H等7个复等位基因控制;通过酶切图谱分析发现蒙古山羊和哈萨克山羊的GOLA-DRB3基因外显子2的154、168和220位碱基表现出多态性。并对基因型频率和等位基因频率进行了统计分析,结果表明,GOLA-DRB3基因的部分基因型频率和等位基因频率在两个群体之间差异显著(P<0.10或P<0.05)或极显著(P<0.01);X2适合性检验结果表明,蒙古山羊和哈萨克山羊的GOLA-DRB3基因外显子2的HaeIII酶切位点均未达到Hardy-Weiberg平衡状态(P<0.01)。
蒙古山羊, 哈萨克山羊, GOLA-DRB3, PCR-RFLP
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【期刊论文】杂种鸡与亲本之间差异表达基因及其与杂种优势的关系*
孙东晓, 王栋, 张沅**, 愈英, 徐桂云, 李俊英
农业生物技术学报,2004,12(5):548~551,-0001,():
-1年11月30日
利用mRNA 差异显示技术,对白洛克肉鸡、中国丝羽乌骨鸡及其正反交组合8周龄肝脏组织的基因表达进行了分析。发现了3个杂种鸡特异表达的基因和1个杂种鸡表达增强的基因,进一步克隆、测序和比对分析初步表明:杂种特异表达的cDNA片断分别为鸡细胞质异柠檬酸脱氢酶基因和2个未知功能的新基因;杂种表达增强的cDNA片断为鸡蛋白酶体亚基p112基因。功能基因和调控基因在杂种鸡的特异表达和增强表达可能与部分屠体性状杂种优势形成有关。
鸡, mRNA差异显示, 杂种特异表达, 杂种增强表达, 杂种优势
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