赖锦盛
1.玉米籽粒的功能基因组学;2.玉米抗虫抗旱基因工程;3.禾本科植物比较基因组学;4.玉米分子育种。
个性化签名
- 姓名:赖锦盛
- 目前身份:
- 担任导师情况:
- 学位:
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学术头衔:
博士生导师, 教育部“新世纪优秀人才支持计划”入选者
- 职称:-
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学科领域:
作物育种学与良种繁育学
- 研究兴趣:1.玉米籽粒的功能基因组学;2.玉米抗虫抗旱基因工程;3.禾本科植物比较基因组学;4.玉米分子育种。
赖锦盛,博士,中国农业大学特聘教授,博士生导师,2007年度 “杜邦青年教授”,2007年度教育部新世纪优秀人才基金获得者。
1996年毕业于中国农业大学植物遗传育种专业,获博士学位。1997-2004年,在美国Rutgers大学Waksman研究所博士后(Postdoc)和研究工作人员(Research Associate)。2005-2006年,在Monsanto公司总部工作, 2006年9月,被聘为中国农业大学特聘教授。
在玉米遗传转化、分子遗传学、比较基因组以及玉米胚乳的功能基因组等方面取得了重要进展:在比较基因组学方面,对玉米亲本自交系间的遗传组成多态性进行了深入研究,首次提出Helitron转座子在玉米种内基因转移中的作用(Lai et al, 2005,PNAS)。对禾本科基因组(玉米、高梁、水稻)进行了系统的比较研究,提出基因转移在打破禾本科基因组微共线性及近缘基因组演化重要作用(Lai et al, 2004a, Genome Research),并对玉米和高梁的亲缘关系提出新的假说(Zwigona, Lai, et al 2004, Genome Research)。在功能基因组方面,对玉米胚乳全长cDNA 测序,玉米转座子突变群体构建等(Lai et al, 2004b, Genome Research)。在转基因方面,获得稳定高赖氨酸玉米(Lai & Messing, 2002, The Plant Journal),2005年获美国和世界专利(专利号:美国6849779;世界WO 0012681)。
目前主要从事以下几个方向的研究:
1.玉米籽粒的功能基因组学
2.玉米抗虫抗旱基因工程
3.禾本科植物比较基因组学
4.玉米分子育种
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成果阅读
642
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成果数
8
【期刊论文】Uneven chromosome contraction and expansionin the maize genome
赖锦盛, Rémy Bruggmann, , Arvind K. Bharti, Heidrun Gundlach, Jinsheng Lai, Sarah Young, Ana C. Pontaroli, Fusheng Wei, Georg Haberer, Galina Fuks, Chunguang Du, Christina Raymond, Matt C. Estep, Renyi Liu, Jeffrey L. Bennetzen, Agnes P. Chan, Pablo D. Rabinowicz, John Quackenbush, W. Brad Barbazuk, Rod A. Wing, Bruce Birren, Chad Nusbaum, Steve Rounsley, Klaus F.X. Mayer, and Joachim Messing
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-1年11月30日
Maize (Zea mays or corn), both a major food source and an important cytogenetic model, evolved from a tetraploidthat arose about 4.8 million years ago (Mya). As a result, maize has extensive duplicated regions within its genome.We have sequenced the two copies of one such region, generating 7.8 Mb of sequence spanning 17.4 cM of the shortarm of chromosome 1 and 6.6 Mb (25.6 cM) from the long arm of chromosome 9. Rice, which did not undergo asimilar whole genome duplication event, has only one orthologous region (4.9 Mb) on the short arm of chromosome3, and can be used as reference for the maize homoeologous regions. Alignment of the three regions allowedidentification of syntenic blocks, and indicated that the maize regions have undergone differential contraction ingenic and intergenic regions and expansion by the insertion of retrotransposable elements. Approximately 9% of thepredicted genes in each duplicated region are completely missing in the rice genome, and almost 20% have movedto other genomic locations. Predicted genes within these regions tend to be larger in maize than in rice, primarilybecause of the presence of predicted genes in maize with larger introns. Interestingly, the general gene methylationpatterns in the maize homoeologous regions do not appear to have changed with contraction or expansion of theirchromosomes. In addition, no differences in methylation of single genes and tandemly repeated gene copies havebeen detected. These results, therefore, provide new insights into the diploidization of polyploid species.
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【期刊论文】Gene movement by Helitron transposons contributes to the haplotype variability of maize
赖锦盛, Jinsheng Lai*, Yubin Li*, Joachim Messing*, and Hugo K. Dooner*†‡
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-1年11月30日
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【期刊论文】DNA Rearrangement in Orthologous Orp Regions of the Maize, Rice andSorghum Genomes
赖锦盛, Jianxin Ma, *, † Phillip SanMiguel, ‡ Jinsheng Lai, § Joachim Messing§ and Jeffrey L. Bennetzen*, †,
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-1年11月30日
The homeologous Orp1 and Orp2 regions of maize and the orthologous regions in sorghum andrice were compared by generating sequence data for 486 kb of genomic DNA. At least three genicrearrangements differentiate the maize Orp1 and Orp2 segments, including an insertion of a single geneand two deletions that removed one gene each, while no genic rearrangements were detected in the maizeOrp2 region relative to sorghum. Extended comparison of the orthologous Orp regions of sorghum andjaponica rice uncovered numerous genic rearrangements and the presence of a transposon-rich region inrice. Only 11 of 27 genes (40%) are arranged in the same order and orientation between sorghum andrice. Of the 8 genes that are uniquely present in the sorghum region, 4 were found to have single-copyhomologs in both rice and Arabidopsis, but none of these genes are located near each other, indicatingfrequent gene movement. Further comparison of the Orp segments from two rice subspecies, japonica andindica, revealed that the transposon-rich region is both an ancient and current hotspot for retrotransposonaccumulation and genic rearrangement. We also identify unequal gene conversion as a mechanism formaize retrotransposon rearrangement.
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【期刊论文】Gene Loss and Movement in the Maize Genome
赖锦盛, Jinsheng Lai, Jianxin Ma, , Zuzana Swigonová, Wusirika Ramakrishna, Eric Linton, Victor Llaca, Bahattin Tanyolac, Yong-Jin Park, O-Young Jeong, Jeffrey L. Bennetzen, and Joachim Messing
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-1年11月30日
Maize (Zea mays L. ssp. mays), one of the most important agricultural crops in the world, originated by hybridizationof two closely related progenitors. To investigate the fate of its genes after tetraploidization, we analyzed thesequence of five duplicated regions from different chromosomal locations. We also compared corresponding regionsfrom sorghum and rice, two important crops that have largely collinear maps with maize. The split of sorghum andmaize progenitors was recently estimated to be 11.9 Mya, whereas rice diverged from the common ancestor of maizeand sorghum ∼50 Mya. A data set of roughly 4 Mb yielded 206 predicted genes from the three species, excludingany transposon-related genes, but including eight gene remnants. On average, 14% of the genes within the alignedregions are noncollinear between any two species. However, scoring each maize region separately, the set ofnoncollinear genes between all four regions jumps to 68%. This is largely because at least 50% of the duplicatedgenes from the two progenitors of maize have been lost over a very short period of time, possibly as short as 5million years. Using the nearly completed rice sequence, we found noncollinear genes in other chromosomalpositions, frequently in more than one. This demonstrates that many genes in these species have moved to newchromosomal locations in the last 50 million years or less, most as single gene events that did not dramatically altergene structure.
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【期刊论文】Close Split of Sorghum and MaizeGenome Progenitors
赖锦盛, Zuzana Swigonova, , Jinsheng Lai, Jianxin Ma, Wusirika Ramakrishna, Victor Llaca, Jeffrey L. Bennetzen, and Joachim Messing
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-1年11月30日
It is generally believed that maize (Zea mays L. ssp. mays) arose as a tetraploid; however, the two progenitor genomescannot be unequivocally traced within the genome of modern maize. We have taken a new approach to investigatethe origin of the maize genome. We isolated and sequenced large genomic fragments from the regions surroundingfive duplicated loci from the maize genome and their orthologous loci in sorghum, and then we compared thesesequences with the orthologous regions in the rice genome. Within the studied segments, we identified 11 genes thatwere conserved in location, order, and orientation. We performed phylogenetic and distance analyses and examinedthe patterns of estimated times of divergence for sorghum and maize gene orthologs and also the time of divergencefor maize orthologs. Our results support a tetraploid origin of maize. This analysis also indicates contemporaneousdivergence of the ancestral sorghum genome and the two maize progenitor genomes about 11.9 million years ago(Mya). On the basis of a putative conversion event detected for one of the genes, tetraploidization must haveoccurred before 4.8 Mya, and therefore, preceded the major maize genome expansion by gene amplification andretrotransposition.
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【期刊论文】Characterization of the Maize EndospermTranscriptome and Its Comparison to theRice Genome
赖锦盛, Jinsheng Lai, Nrisingha Dey, Cheol-Soo Kim, , Arvind K. Bharti, Stephen Rudd, Klaus F.X. Mayer, Brian A. Larkins, Philip Becraft, and Joachim Messing
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-1年11月30日
The cereal endosperm is a major organ of the seed and an important component of the world’s food supply. Tounderstand the development and physiology of the endosperm of cereal seeds, we focused on the identification ofgenes expressed at various times during maize endosperm development. We constructed several cDNA libraries toidentify full-length clones and subjected them to a twofold enrichment. A total of 23,348 high-qualitysequence-reads from 5-and 3-ends of cDNAs were generated and assembled into a unigene set representing 5326genes with paired sequence-reads. Additional sequencing yielded a total of 3160 (59%) completely sequenced,full-length cDNAs. From 5326 unigenes, 4139 (78%) can be aligned with 5367 predicted rice genes and by takingonly the "best hit" be mapped to 3108 positions on the rice genome. The 22% unigenes not present in rice indicatea rapid change of gene content between rice and maize in only 50 million years. Differences in rice and maize genenumbers also suggest that maize has lost a large number of duplicated genes following tetraploidization. The largernumber of gene copies in rice suggests that as many as 30% of its genes arose from gene amplification, which wouldextrapolate to a significant proportion of the estimated 44,027 candidate genes of its entire genome. Functionalclassification of the maize endosperm unigene set indicated that more than a fourth of the novel functionallyassignable genes found in this study are involved in carbohydrate metabolism, consistent with its role as a storageorgan.
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【期刊论文】On the tetraploid origin of the maize genome
赖锦盛, Zuzana Swigonova*, Jinsheng Lai, Jianxin Ma#, Wusirika Ramakrishna##, Victor Llaca###
Comparative and Functional Genomics Comp Funct Genom 2004; 5: 281-284.,-0001,():
-1年11月30日
Data from cytological and genetic mapping studies suggest that maize arose asa tetraploid. Two previous studies investigating the most likely mode of maizeorigin arrived at different conclusions. Gaut and Doebley [7] proposed a segmentalallotetraploid origin of the maize genome and estimated that the two maizeprogenitors diverged at 20.5 million years ago (mya). In a similar study, using largerdata set, Brendel and colleagues (quoted in [8]) suggested a single genome duplicationat 16 mya. One of the key components of such analyses is to examine sequencedivergence among strictly orthologous genes. In order to identify such genes, Laiand colleagues [10] sequenced five duplicated chromosomal regions from the maizegenome and the orthologous counterparts from the sorghum genome. They alsoidentified the orthologous regions in rice. Using positional information of geneticcomponents, they identified 11 orthologous genes across the two duplicated regionsof maize, and the sorghum and rice regions. Swigonova et al. [12] analyzed the 11orthologues, and showed that all five maize chromosomal regions duplicated at thesame time, supporting a tetraploid origin of maize, and that the two maize progenitorsdiverged from each other at about the same time as each of them diverged fromsorghum, about 11.9 mya. Copyright 2004 John Wiley & Sons, Ltd.
maize, sorghum, tetraploidy
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【期刊论文】Increasing maize seed methionine by mRNA stability
赖锦盛, Jinsheng Lai and Joachim Messing*
The Plant Journal (2002) 30(4), 395-402,-0001,():
-1年11月30日
The amino acid methionine is a common protein building block that is also important in other cellularprocesses. Plants, unlike animals, synthesize methionine de novo and are thus a dietary source of thisnutrient. A new approach for using maize as a source of nutrient methionine is described. Maize seeds, amajor component of animal feeds, have variable levels of protein-bound methionine. This variability is aresult of post-transcriptional regulation of the Dzs10 gene, which encodes a seed-speci®c highmethioninestorage protein. Here we eliminate methionine variability by identifying and replacing thecis-acting site for Dzs10 regulation using transgenic seeds. Interestingly, two different mechanisms affectmRNA accumulation, one dependent on and the other independent of the untranslated regions (UTRs) ofDzs10 RNA. Accumulation of chimeric Dzs10 mRNA was not reduced in hybrid crosses and wasuncoupled from genomic imprinting by Dzr1, a regulator of Dzs10. Uniform high levels of Dzs10 proteinwere maintained over ®ve backcross generations of the transgene. The increased level of methionine inthese transgenic seeds allowed the formulation of a useful animal feed ration without the addition ofsynthetic methionine.
Storage proteins,, amino acids,, maize transformation,, post-trans, c, r, i, p, t, ional regulation,, genomicimprinting,, gene expression.,
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