王九峰
个性化签名
- 姓名:王九峰
- 目前身份:
- 担任导师情况:
- 学位:
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学术头衔:
博士生导师, 教育部“新世纪优秀人才支持计划”入选者
- 职称:-
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学科领域:
畜牧科学、动物医学
- 研究兴趣:
个人简介:
王九峰,教授、博士生导师,教育部“新世纪优秀人才支持计划”入选者。目前已在国内外学术刊物上发表论文35篇,其中被SCI收录14篇(其中12篇为第1或通讯作者)。
学习培训经历:
学士/硕士:分别于1992年和1997年在中国农业大学(原北京农业大学)动物医学专业获农学学士和硕士学位;
博士:2000年于中国农业大学动物营养专业获农学博士学位。曾于
1998年9月-2000年1月赴丹麦农业科学院微生物生态室完成博士学位论文;
博士后:2000年9月-2002年9月赴瑞典农业大学进行博士后研究;
合作研究:2003年1月-2004年2月赴瑞典农业大学进行合作研究。
科研项目:
目前在研项目:
1.转基因生物新品种培育重大项目“转基因羊生物安全评价和转基因羊抗性检测”(主持);
2.教育部新世纪优秀人才支持计划项目“教育部新世纪优秀人才支持计划”(主持);
3.生物多样性调查项目“北京百花山自然保护区野生动物生物多样性调查研究”(主持);
4.国家自然科学基金项目“断奶对仔猪肠道结构与功能的影响研究”(主持);
5.临床系科研基金“小动物常见疾病的发病机理研究”(主持);
6.教育部留学归国人员科研启动基金项目“日粮因子对猪肠道粘膜酶活的影响研究”(主持);
7.内蒙古自治区科委项目“以玉米为原料生产奶牛饲料添加剂——EM液科技攻关项目”(副主持)。
近年来主要从事的项目:国家“九五”攻关专题项目1项;国家自然科学基金项目2项;全国博士后科学基金项目1项;丹麦国家食品和农业渔业部重大项目1项;瑞典科学基金项目1项等。
获奖情况:
1.2007年,入选“教育部新世纪优秀人才支持计划”;
2.2007年荣获中国农业大学“优秀教师”称号;
3.2006年荣获“霍英东教育基金会第十届高校青年教师奖”;
4.2006年,主持完成了“动物乳房炎发病机理、诊断和综合防治新技术研究”并通过了教育部成果鉴定(鉴字SW2006 第063号);
5.2005年,由本人申报的“新型猪用饲料添加剂开发研究”项目荣获“第四届大北农科技奖励-成果促进奖”;
6.2000年,“仔猪高效优质预混料配方技术研究”(第6完成人)通过部级成果鉴定((2000)农科果鉴字063号);
7.1999年,由本人参加的科技新产品“1%生长猪预混料配方”荣获“国家重点新产品”称号;
8.1999年,“生长肥育猪预混料配方技术研究”(第5完成人)荣获北京市科技进步三等奖;
9.1997年,被评为中国农业大学优秀硕士毕业生。
同时担任:
1.中国畜牧兽医学会家畜内科学分会常务理事;
2.中国兽医杂志编委;
3.SCI收录杂志“Journal of Dairy Science”,“Journal of Agriculture and Food Science”,“Nutrition”审稿人。
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成果数
8
王九峰, W. C. Sauer, R. Mosenthin, F. Ahrens and L. A. den Hartog
J. Anim. Sci. 1991. 69: 4070-4077,-0001,():
-1年11月30日
Studies were carried out with six barrows (initial BW 50kg) to determine the effect of two fiber sources on the apparent ileal and fecal N and amino acid digestibilities and bacterial N excretion in feces. The pigs, fitted with a simple Tcannula at the distal ileum, were fed three cornstarch-based diets formulated to contain 16% CP from soybean meal according to a repeated 3x3 Latin square design. Diet 1 did not contain additional fiber. Diets 2 and 3 contained 10% powdered cellulose and 10% barley straw (ground through a 2-mm mesh screen), included at the expense of cornstarch, resptively. The pigs were fed 900g at each meal twice daily, 0800 and 2000. Feces were collected for 48h after a 1O d adaptation period. Thereafter, ileal digesta were collected for 24h. Chromic oxide was used as a digestibility marker. With the exception of leucine, the inclusion of fiber did not affect (P>.OS) the ileal digestibilities of the indispensable amino acids. The values for the average of the ileal digestibilities of the indispensable amino acids were 85.3, 82.9, and 83.2% for diets 1, 2, and 3, respectively. On the other hand, the inclusion of fiber decreased (P<.05) the fecal digestibilities of all the indispensable amino acids. The values for the average of the fecal digestibilities of the indispensable amino acids were 91.7, 87.4, and 89.1% for diets 1, 2, and 3, respectively. These decreases resulted from an increase (P.05) in the excretion of bacterial N, which was measured using 2, 6-diaminopimelic acid as a marker. In conclusion, as measured with the ileal analysis method, the inclusion of fiber had little effect on the digestible
Pigs, Fiber, Amino Acids, Digestibility
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【期刊论文】In vitro fermentation of various fiber and starch sources by pig fecal inocula1
王九峰, J. F. Wang*†, Y. H. Zhu†, D. F. Li*, Z. Wang*, and B. B. Jensen†
J. Anim. Sci. 2004. 82: 2615-2622,-0001,():
-1年11月30日
Freeze-dried ileal effluent (1% wt/vol) from cannulated pigs fed rice-based diets with the inclusion of either animal protein (CON), animal protein plus potato starch (PS), animal protein plus sugar beet pulp (SBP), or animal protein plus wheat bran (WB) was incubated anaerobically at pH 6.0 in fermenters containing 5% (wt/vol) fecal slurry comprising mineral salts medium and 50 g/L of fresh feces from pigs fed the same diets as the cannulated pigs. Samples were collected from the fermenters at 0, 2, 4, 12, 24, and 48h during in vitro fermentation for measuring nonstarch polysaccharides (NSP), starch, and short-chain fatty acids (SCFA). Results showed that the major SCFA produced were acetate, propionate, and butyrate. The inclusion of soluble dietary fiber (diet SBP) caused the highest concentrations of acetate, propionate, butyrate, and total SCFA, whereas the increase in the production of propionate resulting from the addition of insoluble dietary fiber (diet WB) only occurred at the initial stages during 48h in vitro fermentation. At all sampling occasions (except for 4h), the levels of butyrate were increased (P<0.01) by resistant starch compared with fiber sources, showing that a higher level of butyrate can be achieved through microbial fermentation by potato starch. Lowered (P<0.05) butyrate concentrations were observed with diet WB during in vitro fermentation. With the inclusion of fiber sources, the energy originating from SCFA was similar to that from NSP disappearance, whereas the values were lower (P<0.05) from NSP disappearance than for SCFA generated without fiber sources supplemented. We conclude that more substrate is available in ileal effluent with the addition of soluble dietary fiber, and an increased level of butyrate could be achieved through microbial fermentation by resistant starch.
Dietary Fiber, In Vitro Fermentation, Pig, Short-Chain Fatty Acids, Starch
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王九峰, J. F. Wang, †, Y. H. Zhu, D. F. Li, M. Wangand B. B. Jensen
Animal Science 2004, 78: 109-117,-0001,():
-1年11月30日
A repeated 4✕4 Latin square design was conducted with eight ileal cannulated castrated pigs to investigate the effect of source of dietary fibre and starch on ileal and faecal microflora and short-chain fatty acid concentrations. Four experimental diets based on cooked rice were supplied with one of two fibre-rich sources (sugar-beet pulp, S; wheat bran, W) and another two diets were prepared with (P) or without (C) potato starch. The experimental periods were 14 days, consisting of 7 days of adaptation to each diet, followed by 4 days of collection of ileal digesta and 3 days of collection of faeces. Ileal digesta were collected daily in a randomized order 0 (just before feeding the morning meal), 2, 4, 6 and 8h after feeding the morning meal. Fresh faecal samples were also collected. Ileal pH was lower in pigs given diet P, 2 and 4h after feeding, respectively. For all four diets ileal pH reached a minimum 4h after feeding. Faecal pH was higher for diets P and W compared with diets C and S. The highest density of ileal coliform bacteria was found 4h after feeding for all diets. Compared with other diets, the inclusion of potato starch resulted in an increased density of ileal enterococci at 0h and an increased density of ileal lactic acid bacteria and lactobacilli, 2 and 6h after feeding, respectively. The density of ileal enterococci reached a maximum 4h after feeding for all diets. With the exception of 8h after feeding, when a higher density of total anaerobes was observed for diet P, no significant differences were seen in the populations of yeasts and total anaerobes between the experimental diets. The concentration of formate in the ileum was high, while low concentrations of acetate, propionate and butyrate were observed for all diets. A lowered level of acetate was found for diet S on all sampling occasions. On a dry-matter basis, the counts of various faecal bacteria were increased by the inclusion of the fibre sources, and a higher level of faecal butyrate was found with the inclusion of potato starch or the fibre sources as compared with diet C, whereas no significant effects on the counts of various bacteria were observed with potato starch supplementation. Overall, the present results indicate that the addition of dietary fibre to pig diets resulted in an enhanced microbial fermentation.
fibre, microbial flora, potato starch, rice, short-chain fatty acids.,
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王九峰, J.F. Wang a, b, D.F. Li a, ∗, B.B. Jensen b, K. Jakobsen b, J.J. Xing a, L.M. Gonga, Y.H. Zhub
Animal Feed Science and Technology 104(2003)95-110,-0001,():
-1年11月30日
The present study was undertaken to study the effect of dietary fibre on gastric microflora, and short-chain fatty acids (SCFA) concentrations at different time after feeding in gestating sows. Three experimental diets consisting of two dietary fibre-rich diets and one concentrated, low dietary fibre diet (C) were used. One fibre-rich diet contained soluble dietary fibre from sugar beet pulp (S) and the other contained insoluble dietary fibre from wheat bran (W). The experiment was carried out as repeated 3×3 Latin squares with six gastric-cannulated gestating sows fed one of the three experimental diets, with experimental periods of 7 days, comprising 3 days of adaptation to each diet, followed by 4 days of collection of gastric digesta. The gastric digesta were evacuated once daily through the gastric cannula in a randomized order 0.5, 1, 2, 3, 5 and 15.5h after feeding the morning meal. The evacuated gastric digesta were quantified and a representative sample collected for measuring pH, dry matter (DM) content, SCFA, density of various bacterial populations (including coliform bacteria, lactic acid bacteria, lactobacilli, enterococci and yeasts), adenosine 5’-triphosphate (ATP), and adenylate energy charge (AEC). With all three diets, gastric pH was highest at 0.5h after feeding, and then fell gradually with increasing time (especially between 0.5 and 2h) after feeding. The changing trend of pH with the three diets was not significantly different. The total counts of coliform bacteria, lactic acid bacteria, lactobacilli, enterococci and yeasts were also highest at 0.5h after feeding, in agreement with gastric pH values. The population of coliform bacteria with diet S decreased significantly compared with diet C or diet W (P<0.05). The concentration of gastric ATP was highest at 15.5h after feeding. The level of acetate had the lowest value at 0.5–1h with diets S and C, but at 2h with diet W. In conclusion, gastric pH was unstable at different time after feeding, and was highest at 0.5h after feeding. The highest populations of various gastric bacteria were found at 0.5h after feeding, irrespective of type of diet. As gastric pH increased, gastric microbial activity was inhibited. The populations of lactic acid bacteria and lactobacilli with the insoluble dietary fibre were always higher than with the soluble dietary fibre at all times after feeding. Soluble dietary fibre increased the level of acetate.
Microflora, Short-chain fatty acid, Dietary fibre, Gestating sows
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王九峰, Jan Erik Lindberg∗, Anna Arvidsson, Jiufeng Wang
Animal Feed Science and Technology 104(2003)121-131,-0001,():
-1年11月30日
Nutrient utilization and performance were studied in two experiments with weaning piglets fed diets based on naked barley cultivars with normal, amylose-rich and amylopectin-rich starch (ST), with or without supplementation of an enzyme preparation with glucanase and xylanase activity. The coefficient of apparent digestibility (CAD) of components in all diets gradually increased from the small intestine to the rectum, although the extent of digestion in the small intestine and caecum was different for the barley cultivars. The diet based on naked normal barley showed a lower CAD (P<0.05) for organic matter (OM), crude protein (CP) and starch (ST) at the small intestine (0.25, 0.12 and 0.72) than the amylose-rich (0.61, 0.64 and 0.87) and amylopectin-rich (0.54, 0.49 and 0.87) barley cultivars. In contrast, at the caecum the naked normal barley showed higher CAD values (P<0.05) for OM, CP and ST (0.48, 0.49 and 0.26) than the amylose-rich (0.15, 0.08 and 0.10) and amylopectin-rich (0.23, 0.21 and 0.11) barley cultivars. There were no cultivar related differences in the extent of digestion at the colon and rectum. The coefficient of total tract apparent digestibility (CTTAD) ofOMandCPwas higher (P<0.05) for the amylose-rich (0.89 and 0.83) and amylopectin-rich (0.89 and 0.80) barley cultivars than for the normal barley cultivar (0.85 and 0.77). However, there were no differences in performance (P>0.05) between diets based the different barley cultivars. Enzyme supplementation of the diets improved the CTTAD of CP (P<0.05), while it had no effect (P>0.05) on the performance. In conclusion, the starch composition of barley appears to have a limited effect on its utilization in piglets. However, the impaired digestibility of the naked normal barley cultivar indicates that there are properties in the hulless grain that imposes limitation to its utilization and which could not be alleviated by enzyme supplementation. Overall, the present results support the contention that the potential for improvement of piglet growth performance by supplementing barley-based diets with cell wall degrading enzymes is limited.
Barley cultivar, Enzyme, Growth, Digestibility, Piglets
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【期刊论文】The influence of diet on the development of swine dysentery upon experimental infection
王九峰, R. H. Lindecrona†, T. K. Jensen, B. B. Jensen, T. D. Leser, W. Jiufeng and K. Møller
Animal Science 2003, 76: 81-87,-0001,():
-1年11月30日
The purpose of the present study was to evaluate the effect of fermented liquid food (FLF) and the addition of lactic acid to a diet based on wheat and barley on the development of swine dysentery in pigs experimentally infected with a Danish field isolate of Brachyspira hyodysenteriae. Furthermore, to confirm if low non-starch polysaccharide (NSP)-containing diets reduce swine dysentery the effect of different dietary levels of NSP and resistant starch (RS) was evaluated. These diets were based on cooked rice and animal protein, cooked rice and potato starch, cooked rice and wheat bran, or cooked rice and sugar-beet pulp. The experiment was designed as a randomized-block trial and was performed in triplicate including a total of 192 pigs. After feeding the diets for 2 weeks, six pigs in each group were challenged orally with B. hyodysenteriae and observed for another 4 weeks. After challenge, swine dysentery was observed in all feeding groups. The incidence of disease varied between 94% (rice/wheat bran) and 44% (FLF). The effect of diet on faecal shedding of B. hyodysenteriae was statistically significant (P<0.05). Feeding a diet based on cooked rice with a low content of NSP and RS, did not prevent the development of swine dysentery upon experimental challenge, and increasing the level of NSP or RS did not result in a higher incidence of disease or faecal shedding of B. hyodysenteriae. The incidence of swine dysentery in the FLF group was significantly lower (P<0.05) compared with all other feeding groups, except for the lactic acid group. In conclusion, a low level of NSP or RS in the diet did not prevent the development of swine dysentery. Furthermore, the lowest incidence of disease was observed in the FLF group, even though this diet has a high content of NSP. The addition of organic acids to the food was not able to reduce infection with B. hyodysenteriae.
Brachyspira hyodysenteriae, food additives, pigs, swine dysentery.,
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王九峰, J.F. Wang a, B.B. Jensen a, H. Jørgensen a, D.F. Li b, J.E. Lindberg c, ∗
Animal Feed Science and Technology 102(2002)125-136,-0001,():
-1年11月30日
A repeated 4×4 Latin square design was performed with eight ileal cannulated castrates to study the effect of source of starch and fibre on apparent ileal and faecal digestibility, and crude protein (CP) and fat balance. The experimental diets were based on cooked rice (C) and cooked rice with addition of raw potato starch (P), sugar beet pulp (S) and wheat bran (W). The inclusion of potato starch reduced (P<0.05) the ileal digestibility of organic matter (OM) and starch, and the total tract digestibility of CP. The ileal digestibility of potato starchwas calculated to be 0.75. Inclusion of fibre sources (sugar beet pulp and wheat bran) in the basal diet resulted in a reduction (P<0.05) in the ileal and faecal digestibility of dietary components (except for starch) and energy. While there were no differences (P>0.05) in the ileal digestibility of dietary components and energy between fibre sources, the total tract digestibility of dietary components (except for CP and starch) and energy were lower (P<0.05) for the insoluble fibre source (wheat bran). With the exception of mannose and uronic acid the ileal digestibility of individual sugars were unaffected (P>0.05) by diet composition. The inclusion of wheat bran negatively influenced the faecal digestibility of individual sugars (except for xylose) and non-starch polysaccharides. Nitrogen retention was unaffected (P>0.05) by diet composition, while fat accretion tended (P<0.10) to be lower in diet C. The latter was due to a low feed and energy intake.
Rice, Potato starch, Sugar beet pulp, Wheat bran, Dietary fibre
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