金利通
化学修饰电极、生物电化学传感器、味觉传感器、色谱电化学、环境分析化学新方法、环境污染监测及防治对策、分析仪器的开发等
个性化签名
- 姓名:金利通
- 目前身份:
- 担任导师情况:
- 学位:
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学术头衔:
博士生导师
- 职称:-
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学科领域:
物理化学
- 研究兴趣:化学修饰电极、生物电化学传感器、味觉传感器、色谱电化学、环境分析化学新方法、环境污染监测及防治对策、分析仪器的开发等
金利通: 华东师范大学终身教授,博士生导师。曾任华东师范大学化学系主任,华东师范大学化学与生命科学学院院长,《化学教学》杂志主编,《华东师范大学学报》杂志副主编。现任中国仪器仪表协会电分析专业委员会秘书长,全国高等师范院校分析化学研究会理事长,生物电化学研究所所长。◆ 科研情况:金利通教授承担了国家“十五”重大科技攻关项目、国家自然科学基金重点项目、国家高技术研究发展计划“863”项目、国家“九五”重点科技攻关等国家级课题以及上海市纳米专项、光科技专项、科学仪器专项等上海市重点科学攻关项目,还承担了8项国家自然科学基金面上项目,主要研究领域包括:化学修饰电极、生物电化学传感器、味觉传感器、色谱电化学、环境分析化学新方法、环境污染监测及防治对策、分析仪器的开发等。在国内外学术期刊上发表论文350多篇,其中100多篇发表在SCI收录期刊上。
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2756
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成果阅读
452
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成果数
10
【期刊论文】Immobilization of uricase on ZnO nanorods for a reagentless uric acid biosensor
金利通, Fenfen Zhang a, Xiaoli Wang a, Shiyun Ai a, Zhengdong Sun a, Qiao Wan a, Ziqiang Zhu b, Yuezhong Xian a, Litong Jin a, ∗, Katsunobu Yamamoto c
Analytica Chimica Acta 519(2004)155-160,-0001,():
-1年11月30日
A reagentless uric acid (UA) biosensor based on uricase immobilized on ZnO nanorods was developed. Direct electrochemistry and thermal stability of immobilized uricase were studied. The ZnO nanorods derived electrode retained the enzyme bioactivity and could enhance the electron transfer between the enzyme and the electrode. This sensor showed a high thermal stability up to 85℃ and an electrocatalytic activity to the oxidation of uric acid without the presence of an electron mediator. The electrocatalytic response showed a linear dependence on the uric acid concentration ranging from 5.0×10-6 to 1.0×10-3mol L-1 with a detection limit of 2.0×10-6mol L−1 at 3σ. The apparent KappM value for the uric acid sensor was estimated to be 0.238mM, showing a high affinity.
Reagentless biosensor, ZnO nanorods, Uricase, Uric acid
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金利通, Guoyue Shi, Fang Xu, Hongguo Zhou, Lanqun Mao, Litong Jin*
Analytica Chimica Acta 386(1999)123-127,-0001,():
-1年11月30日
Microdialysis sampling was combined with flow-injection analysis-electrochemical detection (FIA-ECD) to determine the interactions between streptomycini sulfas (STS) and bovine serum albumin (BSA). In the mobile phase of NaOH (pH=13), a Ni-Ti alloy electrode was used as working electrode. At this electrode good electrocatalytical oxidation of streptomycini sulfas occurs with a detection limit of 8.0×10-7mol/l and a linear concentration range 1.0×10-6-1.0×103mol/l at+500meV (vs. Ag/AgCl). Microdialysis is an excellent complement to directly monitor chemical events in different organs in vivo. This method has been widely used for many fields. We applied microdialysis to determine the interactions of drug-protein.Streptomycini sulfas and BSA were mixed at different molar ratios and incubated at 37℃ in a water-bath. The microdialysis probe was then used to sample the mixed STS-BSA solution at a perfusion rate of 1.0μl/min. The concentration of unbound streptomycini sulfas in the microdialysate was determined by FIA-ECD. Relative recovery (R) of streptomycini sulfas, determined in vitro under similar conditions, was approximately 16.0% at 1.0μl/min. The estimated association constant (K) and the number of the binding sites (n) on one molecule of BSA were 5.45×103 (mol/l)-1 and 1.21, respectively. The method provided a fast and simple technique for the study of drug-protein interactions.
Flow-injection analysis, Electrochemical detection, Drug-protein interactions, Microdialysis
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【期刊论文】Fabrication of an ultramicrosensor for measurement of extracellular myocardial superoxide
金利通, Jian Xue, Yuezhong Xian, Xiangyang Ying, Junshui Chen, Lin Wang, Litong Jin*
Analytica Chimica Acta 405(2000)77-85,-0001,():
-1年11月30日
A novel superoxide (O2-) ultramicrosensor based on copper/platinum microparticles and electropolymerized pyrrole was fabricated for the measurement of extracellular myocardial superoxide. The Cu/Pt-PPy modified ultramicrosensors were evaluated, for the first time, as superoxide sensor. The amperometric response to superoxide was monitored at the potential of 0.45meV (versus saturated calomel electrode (SCE)) in Hank's balanced salt solution (HBSS). The sensor proved were proved to have a high sensitivity, selectivity and short response time. The detection limit is 24 (DL) of the sensors is 24mol/l (S/N of 3). The life period (at least 1 month) of sensors is longer than that of enzyme electrodes. The potential interference from some endogenous electroactive substances in biological tissues, such as hydrogen peroxide (H2O2), uric acid (UA), neurotransmitters and their metabolites, at the concentrations higher than those in biological systems, could be eliminated by further coating the Cu/Pt modified electrode with a polymer film. The method was applied to the measurement of superoxide production in a biologically relevant model system and in rat myocardial cells (MCs).
Superoxide ultramicrosensors, Cu/, Pt-PPy, Myocardial cells
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【期刊论文】Electrochemical degradation of bromopyrogallol red in presence of cobaltions
金利通, Junshui Chen, Meichuan Liu, Jidong Zhang, Yuezhong Xian, Litong Jin *
Chemosphere 53(2003)1131-1136,-0001,():
-1年11月30日
This paper summarizes the results of a degradation test of bromopyrogallol red (BPR) and textile dye wastewater (TDW) with a conventional three-electrode potentiostatic system in the presence of cobalt ions (electro Co2+-H2O2 system). H2O2, produced by the two-electron reduction of O2 at the cathode, would react with Co2+ ions, leading to the generation of hydroxyl radicals (
Bromopyrogallol red, Degradation, Hydroxyl radical, Electro-Fenton s reagent, Cobaltions
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金利通, Wenliang Sun a, Song Zhang a, Xinrong Lin b, Litong Jin a, Songling Jin b, Jiaqi Deng b, Jilie Kong b, *
Journal of Electroanalytical Chemistry 469(1999)63-71,-0001,():
-1年11月30日
A new type of chemically modified electrode (CME) was fabricated by electrodeposition of palladium (Ⅱ)-substituted Dawson type heptadecatungstodiphosphate, K8 [P2W17O61Pd(H2O)] (abbreviated as P2W17Pd in the following), onto a carbon fiber microelectrode (CFME). A pair of waves was observed on the P2W17Pd CFME, which is ascribed to the redox process of the palladium center in the heteropolytungstate. After continuous potential scanning for 30 min in a pH 4.0 buffer, 92% of the original electrode response remained for the P2W17Pd CFME. The P2W17Pd CFME had high electrocatalytic activity for nitrite reduction and exhibited good reproducibility and stability. The catalytic peak current was found to be linear with the nitrite concentration in the range of 1.0×10-7~1.2×10-3mol l-1 (at25℃) with a correlation coefficient of 0.9886. The detection limit (signal: noise 3) was found to be 2.0×10-8mol l-1. The response time of the microsensor for nitrite measurement was less than 15s. For 10 parallel measurements of 1.0×10-5 mol l-1 nitrite, the relative standard deviation (RSD) was found to be 4.5%. The sensitivity of the microsensor was 0.57nA (μmol l-1)-1. The P2W17Pd CME was applied successfully as an electrochemical detector (ECD) to determine the nitrite level in rat brain by flow injection analysis (FIA) coupled with microdialysis sampling. The linear range was over three orders of magnitude and the detection limit was 3.0 pmol for nitrite determination. The mechanism of the catalytic reaction was also addressed.
Carbon fiber microelectrode, Palladium, Dawson type heteropolytungstate, Chemically modified electrode, Nitrite reduction
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金利通, Junshui Chen, Meichuan Liu, Li Zhang, Jidong Zhang, Litong Jin*
Water Research 37(2003)3815-3820,-0001,():
-1年11月30日
A novel composite reactor was prepared and studied towards the degradation of organic pollutants in this work. In the reactor, a UV lamp was installed to provide energy to excite nano TiO2, which served as photocatalyst, leading to the production of hole-electron pairs, and a three-electrode electrolysis systemwas used to accumulate H2O2 which played an important role in the degradation process. The reactor was evaluated by the degradation process of rhodamine 6G (R-6G), and the data obtained in the experiments showed that the combination of the photochemical and electrochemical system raised the degradation rate of R-6G greatly; the working mechanism of the reactor was also discussed in the article. The prepared reactor was also utilized to treat polluted water fromdyeing and printing process. After continuous treatment for 0.5h, chemical oxygen demand biochemical oxygen demand, quantity of bacteria and ammonia nitrogen of the polluted water were reduced by 93.9%, 87.6%, 99.9% and 67.5%, respectively, which indicated that the method used here could be used for effective organic dyes degradation.
Photocatalysis, Hole-electron pairs, Hydroxyl radical, TiO2, Rhodamine 6G
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金利通, Jian Xue, †, Xiangyang Ying, Junshui Chen, Yuezhong Xian, and Litong Jin*, Jiye Jin
Anal. Chem. 2000, 72, 5313-5321,-0001,():
-1年11月30日
The research studied the concentration variation of peroxynitrite anion (O=N-O-O-) released from cultured neonatal myocardial cells induced by ischemia/reperfusion and studied the protective effect of melatonin on the injury. For this purpose, amperometry peroxynitrite ultramicrosensors (UMS) were fabricated and constructed by electropolymerizing inorganic macromolecular film of tetraaminophthalocyanine manganese (Ⅱ) and coating chemically with poly (4-vinylpyridine). Under optimum conditions, the UMS showed high selectivity and sensitivity to peroxynitrite determination with a calculated detection limit of 1.8×10-8mol/L (S/N of 3). The detection of peroxynitrite was based on electrocatalytic reduction of peroxynitrite. The mechanism of catalysis was also discussed. The UMS should be promising for in vivo measurement of peroxynitrite without interference or fouling. Peroxynitrite released from myocardial cells both in the ischemic period and in the reperfusion period was measured directly. This approach may lead to important information for myocardial cells on the mechanism of injury and prospective treatments of medicine such as melatonin.
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金利通, Fen-Fen Zhang a, Qiao Wan a, Xiao-Li Wang a, Zheng-Dong Sun a, Zi-Qiang Zhu b, Yue-Zhong Xian a, Li-Tong Jin a, *, Katsunobu Yamamoto c
Journal of Electroanalytical Chemistry 571(2004)133-138,-0001,():
-1年11月30日
In this paper, microdialysis sampling was combined with an enzymatic assay of glucose level in rat brain. A novel amperometric glucose biosensor based on ferrocene-doped silica (FcDS) nanoparticles conjugated with a biopolymer chitosan (CHIT) membrane was developed. These uniform FcDS nanoparticles (about 15
Ferrocene-doped silica nanoparticle, Electron transfer mediator, Glucose oxidase, Microdialysis sampling, Rat brain
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金利通, Wen Zhang a, Fangli Wan a, Yunfeng Xie a, Jing Gua, Jian Wang b, Katsunobu Yamamoto c, Litong Jin a, ∗
Analytica Chimica Acta 512(2004)207-214,-0001,():
-1年11月30日
The fabrication and application of a novel electrochemical detection (ED) system with the functionalized multi-wall carbon nanotubes (MWNTs) chemically modified electrode (CME) for liquid chromatography (LC) were described. The electrochemical behaviors of dopamine (DA) at the CME were investigated by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Itwas found that the CME exhibited efficiently electrocatalytic effect on the current responses of (R)-salsolinol ((R)-Sal), (R)-N-methylsalsolinol ((R)-NMSal), and monoamine neurotransmitters with high sensitivity, stability, and long-life of activity. In LC-ED, all (R)-Sal, (R)-NMSal, DA, norepinephrine (NE), 3-methoxy-4-hydroxyphenylglycol (MHPG), 3, 4-dihydroxyphenylacetic acid (DOPAC), 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA), and homovanillic acid (HVA) had good and stable current responses at the CME. The linear ranges of the nine analytes were over three orders of magnitude (R2>0.995). The application of this method coupled with microdialysis sampling for the determination of (R)-Sal, (R)-NMSal, and monoamine neurotransmitters in Parkinson's patients' cerebrospinal fluid was satisfactory.
Multi-wall carbon nanotube, (, R), -Sal, (, R), -NMSal, Monoamine neurotransmitters, Parkinson', s disease, Cerebrospinal fluid, HPLC-ED
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【期刊论文】A novel nano-Au-assembled gas sensor for atmospheric oxygen determination
金利通, Hui Li, Jingxia Wen, Qi Cai, Xiaoli Wang, Jiming Xu and Litong Jin*
Analyst, 2001, 126, 1747-1750,-0001,():
-1年11月30日
A novel nano-Au-assembled gas sensor was fabricated and first used to determine atmospheric oxygen. The special characteristics of nano-Au resulted in a high catalytic activity for the reduction of oxygen. On determining 20.9% oxygen by cyclic voltammetry, on a nano-Au-assembled Pt disk electrode the overpotential decreased greatly and the reduction current of oxygen increased to about 4.4 times that on a bare gas sensor. For the determination of atmospheric oxygen by using this novel nano-Au-assembled gas sensor with the amperometricit curve method, the linear range was from 0.4 to 30.0%, the linear equation was y=1.11x-0.63 with a correlation coefficient of 0.9931, the sensitivity was 1.11
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