吴晓磊
环境生物技术,污染治理和资源化,生物质能转化和利用,流域环境修复和生态重建
个性化签名
- 姓名:吴晓磊
- 目前身份:
- 担任导师情况:
- 学位:
-
学术头衔:
博士生导师
- 职称:-
-
学科领域:
环境工程学
- 研究兴趣:环境生物技术,污染治理和资源化,生物质能转化和利用,流域环境修复和生态重建
吴晓磊
能源与资源系教授
教育经历:
1991.9-1995.7 清华大学环境工程系 博士
1990.9-1991.7 清华大学环境工程系 硕士研究生
1985.9-1990.7 清华大学环境工程系 学士
工作经历:
2007.9-至今 北京大学工学院能源与资源系 教授,博导
2006.6-2007.8 清华大学环境科学与工程系环境生物技术中心 主任,教授
2003.7-2006.5 清华大学环境科学与工程系环境生物技术中心 主任,副研究员
2002.2-2003.6 德国马普学会陆生微生物研究所(Max-Planck Institute for Terrestrial Microbiology) 博士后
2001.2-2002.2 德国Marburg大学放射医学系 实验室主任
1998.11-2001.2 德国马普学会陆生微生物研究所(Max-Planck Institute for Terrestrial Microbiology) 博士后
1998.4-1998.10 日本国茨城科学技术振兴财团 高级研究员
1995.8-1998.3 日本国环境厅 Eco-Frontier Fellow
日本国环境厅国立环境研究所 共同研究员
日本国际环境研究协会 AIRIES Fellow
社会兼职:
2006-至今 国际水协会(IWA)中国理事会 秘书
2006-至今 中国城镇供水排水协会 理事
2006-至今 中国微生物学会环境微生物专业委员会 委员
2005-至今 中国科学院南京土壤研究所 客座研究员
2006-至今 土壤与农业可持续发展国家重点实验室 学术委员会委员
2007-2011 提高采收率国家重点实验室 学术委员会委员
研究领域:
环境生物技术,污染治理和资源化,生物质能转化和利用,流域环境修复和生态重建
获得荣誉:
2007 首届“北大泰普工学讲座教授”
2006 中国环境科学学会,第六届“优秀环境科技工作者奖”
2002 教育部“春晖计划”奖
2002 中国科学院王宽诚教育基金会王宽诚科研奖
1995 国家教育委员会科学技术进步三等奖
-
主页访问
1501
-
关注数
0
-
成果阅读
809
-
成果数
14
【期刊论文】Oceanicola nanhaiensis sp. nov., isolated from sediments of the South China Sea
吴晓磊, Jun Gu, Bin Guo, Ya-Nan Wang, Su-Lin Yu, Ryuhei Inamori, Ri Qu, , Yu-Guang Ye and Xiao-Lei Wu
International Journal of Systematic and Evolutionary Microbiology (2007), 57, 157-160,-0001,():
-1年11月30日
A Gram-negative, non-motile, rod-shaped bacterium, strain SS011B1-20T, was isolated from sediments of the South China Sea. Growth occurred at NaCl concentrations between 0 and 10% and at temperatures between 10 and 37 6C. Strain SS011B1-20T contained Q-10 as the major respiratory quinone and C18: 1v7c (81.2%), C16: 0 (7.0%) and C18: 1 methyl (4.3%) as the predominant fatty acids. The G+C content of the genomic DNA was 64.7 mol%. A phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain SS011B1-20T belonged to a clade within the genus Oceanicola in the Alphaproteobacteria, the highest sequence similarities being found with respect to Oceanicola batsensis (96.3%) and with Oceanicola granulosus (94.9%). Strain SS011B1-20T could be clearly distinguished from other Oceanicola species on the basis of the genotypic, phenotypic and phylogenetic data. Thus, it is proposed that strain SS011B1-20T represents a novel species of the genus Oceanicola, with the name Oceanicola nanhaiensis sp. nov. The type strain is SS011B1-20T (=LMG 23508T=CGMCC 1.6293T).
-
39浏览
-
0点赞
-
0收藏
-
0分享
-
85下载
-
0评论
-
引用
吴晓磊, Jun Gu, Hua Cai, Su-Lin Yu, Ri Qu, Bin Yin, Yu-Feng Guo, Jin-Yi Zhao and Xiao-Lei Wu
International Journal of Systematic and Evolutionary Microbiology (2007), 57, 250-254,-0001,():
-1年11月30日
Two novel strains, SL014B61AT and SL014B11A, were isolated from an oil-polluted saline soil from Gudao in the coastal Shengli Oilfield, eastern China. Cells of strains SL014B61AT and SL014B11A were motile, Gram-negative and rod-shaped. Growth occurred at NaCl concentrations of between 0 and 15% and at temperatures of between 10 and 45 6C. Strain SL014B61AT had Q9 as the major respiratory quinone and C16: 0 (21.2%), C18: 1v9c (20.3%), C16: 1v7c (7.3%) and C16: 1v9c (6.4%) as predominant fatty acids. The G+C content of the DNA was 57.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SL014B61AT belonged to the genus Marinobacter in the class Gammaproteobacteria. Strain SL014B61AT showed the highest 16S rRNA gene sequence similarity with Marinobacter bryozoorum (97.9%) and showed 97.8% sequence similarity to Marinobacter lipolyticus. DNA–DNA relatedness to the reference strains Marinobacter bryozoorum and Marinobacter lipolyticus was 35.5% and 33.8%, respectively. On the basis of these data, it is proposed that strains SL014B61AT and SL014B11A represent a novel species, Marinobacter gudaonensis sp. nov. The type strain is strain SL014B61AT (=DSM 18066T=LMG 23509T=CGMCC 1.6294T).
-
59浏览
-
0点赞
-
0收藏
-
0分享
-
128下载
-
0评论
-
引用
【期刊论文】Halomonas gudaonensis sp. nov., isolated from a saline soil contaminated by crude oil
吴晓磊, Ya-Nan Wang, Hua Cai, Su-Lin Yu, Zhi-Yao Wang, Jie Liu and Xiao-Lei Wu
International Journal of Systematic and Evolutionary Microbiology (2007), 57, 911-915,-0001,():
-1年11月30日
Two moderately halophilic strains, SL014B-69T and SL014B-62A2, were isolated from a saline soil contaminated with crude oil in Gudao in the coastal Shengli oilfield in China; the isolates were Gram-negative, rod-shaped and carried lateral flagella. Growth occurred at NaCl concentrations of 1-20%(w/v), at temperatures of 10-42 6Cand at pH 8.0-9.0. Strain SL014B-69T hadC18: 1v7c (28.61%), C19: 1 cyclo v7c (27.97%), C16: 0 (19.66%) and C12: 0 3-OH (8.87%) as the predominant fatty acids and Q9 as the major ubiquinone, with the G+C content of genomic DNA being 64.0 mol%. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the two strains belonged to genus of Halomonas in the Gammaproteobacteria, with the highest 16S rRNA gene sequence similarities of 96.4% with Halomonas campisalis ATCC 700597T and 96.0%with Halomonas desiderata FB2T. DNA-DNA relatedness of strain SL014B-69T with strain SL014B-62A2, H. campisalis ATCC 700597T and H. desiderata DSM 9502T was 97.4, 42.9 and 36.8%, respectively. On the basis of these data, a novel species of the genus Halomonas, Halomonas gudaonensis sp. nov., is proposed for strain SL014B-69T and SL014B-62A2. The type strain is SL014B-69T (=LMG 23610T=CGMCC 1.6133T).
-
51浏览
-
0点赞
-
0收藏
-
0分享
-
126下载
-
0评论
-
引用
吴晓磊, Lei Cheng, Tian-Lei Qiu, Xiao-Bo Yin, Xiao-Lei Wu, Guo-Quan Hu, Yu Deng and Hui Zhang
International Journal of Systematic and Evolutionary Microbiology (2007), 57, 2964-2969,-0001,():
-1年11月30日
A thermophilic, methylotrophic methanogen, strain ZC-1T, was isolated from the Shengli oilfield, China. Cells of strain ZC-1T were motile cocci, 0.7-1.0 mm in diameter and always occurred in clusters of two to four cells. Lysis-susceptibility experiments and analysis of transmission electron micrographs of strain ZC-1T suggested the presence of a proteinaceous cell wall. Strain ZC-1T used methanol, methylamine and trimethylamine as substrates for methanogenesis. Optimal growth, with a doubling time of around 5 h, occurred at pH 6.0-6.5, 65 6C, 0.3-0.5 M NaCl and 0.05-0.20 M MgCl2. The DNA G+C content of this organism was 56 mol%. Analysis of 16S rRNA gene sequence and the inferred amino acid sequence of the mcrA gene of strain ZC-1T indicated that it is related specifically to members of the family Methanosaetaceae (90.6 and 76.6% sequence similarity, respectively). However, strain ZC-1T failed to grow with acetate as substrate for methanogenesis, which is a special characteristic of the family Methanosaetaceae. Based on these phenotypic and phylogenic characteristics, strain ZC-1T is proposed to represent a novel genus and species, for which the name Methermicoccus shengliensis gen. nov., sp. nov. is proposed. The type strain is ZC-1T (5CGMCC 1.5056T5DSM 18856T). Methermicoccaceae fam. nov. is also proposed.
-
109浏览
-
0点赞
-
0收藏
-
0分享
-
110下载
-
0评论
-
引用
吴晓磊, Ya-Nan Wang, Hua Cai, Chang-Qiao Chi, An-Huai Lu, Xian-Gui Lin, , Zheng-Feng Jiang and Xiao-Lei Wu, Correspondence Xiao-Lei Wu, xiaolei
International Journal of Systematic and Evolutionary Microbiology (2007), 57, 1222-1226,-0001,():
-1年11月30日
A moderately halophilic bacterium, designated strain SL014B-85T, was isolated from a crude-oil-contaminated saline soil from Shengli oilfield, Shandong Province, China. Cells were Gram-negative, aerobic, short rods with lateral flagella. Growth occurred at NaCl concentrations of 0-15% (optimum 5-15%), at 10-42℃ (optimum 30℃) and at pH 8.0-9.0 (optimum pH 8.5). The only respiratory quinone was Q9, and the main cellular fatty acids were C18: 1v7c, C16: 0 and C19: 0 cyclo v8c. The G+C content of the DNA was 66.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SL014B-85T belonged to the genus Halomonas in the Gammaproteobacteria, with highest sequence similarity of 98.1 and 97.8% to Halomonas alimentaria DSM 15356T and Halomonas ventosae DSM 15911T, respectively. DNA-DNA relatedness values were below 40% with members of closely related Halomonas species. Results of phenotypic, biochemical and phylogenetic analyses revealed that strain SL014B-85T could be classified as representing a novel species of the genus Halomonas, for which the name Halomonas shengliensis sp. nov. is proposed. The type strain is SL014B-85T (=CGMCC 1.6444T=LMG 23897T).
-
48浏览
-
0点赞
-
0收藏
-
0分享
-
226下载
-
0评论
-
引用
吴晓磊, Bin Guo, Jun Gu, Yu-Guang Ye, Yue-Qin Tang, Kenji Kida and Xiao-Lei Wu, Correspondence Xiao-Lei Wu
International Journal of Systematic and Evolutionary Microbiology (2007), 57, 1970-1974,-0001,():
-1年11月30日
A Gram-negative, motile, non-spore-forming and moderately halophilic ellipsoid-shaped marine coccobacillus, designated strain SS011B1-4T, was isolated from benthic sediment of the South China Sea. Optimum growth occurred at 30-37 6C, pH 7.5-8.0 and 4-8% (w/v) NaCl. Strain SS011B1-4T utilized a variety of organic substrates as sole carbon sources, but did not utilize toluene, n-tetradecane or crude oil. Strain SS011B1-4T had ubiquinone-9 as the major respiratory quinone and C18: 1v9c, C16: 0 and C12: 0.3-OH as the predominant fatty acids. The genomic DNA G+C content was 62.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SS011B1-4T belonged to the genus Marinobacter of the Gammaproteobacteria. The results of the phenotypic, phylogenetic and genomic analyses revealed that strain SS011B1-4T represents a novel species of the genus Marinobacter. The name Marinobacter segnicrescens sp. nov. is therefore proposed, with strain SS011B1-4T (5LMG 23928T5CGMCC 1.6489T) as the type strain.
-
128浏览
-
0点赞
-
0收藏
-
0分享
-
90下载
-
0评论
-
引用
吴晓磊, 刘芳, 叶思源, 汤岳琴, 滝川清, 木田建次, 吴晓磊**
应用与环境生物学报,2007,13(5): 691~696,-0001,():
-1年11月30日
采用末端限制性片段长度多态性分析(T2RFLP)技术和16S rDNA克隆文库的方法, 分析了黄河三角洲滨海湿地土壤不同深度细菌和古菌的群落结构。研究表明, 随着深度的增加, 细菌群落的多样性下降, 而古菌群落多样性则有上升的趋势, 且土壤的细菌和古菌群落结构都呈现出规律的层状分布。该土壤包括各种硫酸盐还原菌、产甲烷古菌、光合细菌等丰富的细菌和古菌资源。
滨海湿地, 微生物群落结构, 末端限制性片段长度多态性分析, 16S rDNA 克隆文库, 种群多样性
-
35浏览
-
0点赞
-
0收藏
-
0分享
-
125下载
-
0评论
-
引用
吴晓磊, 郭斌, 吴晓磊*, 钱易
微生物学报,2006,46(3):504~507,-0001,():
-1年11月30日
目前自然界中只有极少部分微生物能够得到培养,严重阻碍了对微生物生命活动规律的研究和微生物资源的开发。改进传统培养方法,采用新型培养技术,提高微生物可培养性,大量培养自然界中存在的微生物,从而更全面、准确地了解微生物细胞的生命规律、获悉微生物群落中各种微生物之间的动态相互作用和相互协调的规律,对环境微生物工艺进行准确地设计、精细地调控和高效地利用。简要介绍了微生物不可培养的原因,系统总结了有关提高微生物可培养性方法的最新研究进展,提出研究中存在的问题,并阐述了模拟自然环境条件、强调微生物相互关系是提高微生物可培养性的关键。
微生物可培养性, 纯培养技术, 群体感应
-
54浏览
-
0点赞
-
0收藏
-
0分享
-
74下载
-
0评论
-
引用
【期刊论文】油田回注水中硫酸盐还原原核生物的快速检测和群落结构分析
吴晓磊, 曾景海, 吴晓磊*, 赵桂芳, 钱易
环境科学,2006,27(5):972~976,-0001,():
-1年11月30日
利用荧光原位杂交(Fluorescence in situ hybridization, FISH) 技术,采用荧光标记的rRNA 探针及其组合对胜利油田胜利采油厂回注水中硫酸盐还原原核生物(Sulfate reducing prokaryotes, SRPs, 包括硫酸盐还原细菌和硫酸盐还原古菌)进行检测,分析了该回注水中SRPs 群落结构。结果表明:SRPs在胜利油田回注水中具有极高的种群多样性,广泛分布于4个细菌门和1个古菌门;总数可达2.86×104个/mL,占回注水中总微生物细胞的20%左右;其中优势菌属为脱硫弧菌属(Desulf ovibrio)和脱硫肠状菌属(Desulf otomaculum), 分别占回注水微生物总细胞数的8.71%(±4.45%),和12.15% (±3.90%)Desulfobacterales 和Syntrophobacterales这2个目中的SRPs, Thermodesulf obacteriales 以及Thermodesulf ovibro属的SRPs分别占样品微生物总量的7159%(±2.92%),3.57%(±1.39%)和2.32%(±0.80%)。除此之外,也检测到了占微生物总量4.29%(±1.75%)的A rchaeoglobus 属的SRPs, 证明了古菌类SRPs是回注水中一个不容忽视的硫酸盐还原微生物种群。FISH法能够快速、准确地检测回注水中SRPs数量,解析SRPs群落结构。
荧光原位杂交, 硫酸盐还原原核生物, 基因探针, 群落结构, 绝迹稀释法
-
54浏览
-
0点赞
-
0收藏
-
0分享
-
108下载
-
0评论
-
引用
【期刊论文】油井采出液中硝酸盐还原菌的分离培养及对硫酸盐还原的抑制*
吴晓磊, 谭燕, 程杰成, 屈睿, 吴晓磊**
应用与环境生物学报,2007,13 (3):390~394,-0001,():
-1年11月30日
采用油井采出液培养基和加入无机盐成分的改良油井采出液培养基,对大庆油田萨北过渡带油井采出液中的细菌进行分离培养及初步鉴定,比较了两种情况下培养出的具有硝酸盐和/或亚硝酸盐还原,以及/或反硝化能力菌群结构的差异。利用采出液培养基培养出一组新的微生物菌株,并且分离的硝酸盐和/或亚硝酸盐还原菌,以及/或反硝化细菌(Nitrate/nitrite reducing bacteria, denitrifying bacteria, NRDB)比例明显高于无机盐- 采出液培养基;但培养基中无机盐成分的添加,提高了可培养NRDB的群落生物多样性。仅仅向油井采出液中直接投加硝酸盐作为电子受体,对其中硝酸盐还原、亚硝酸盐还原和反硝化微生物(NRDB)的激活作用以及产抑制硫化物产生的能力有限,而同时加入分离自采出液的NRDB和硝酸盐则对硫酸盐还原菌(SRB)的生长和产硫化物活性都产生了明显的抑制。但是NRDB与硝酸盐同时投加对不同SRB的抑制效果并不相同,导致了SRB群落结构的变化。
微生物可培养性, 荧光原位杂交(, F ISH), , 硝酸盐还原菌, 硫酸盐还原菌
-
85浏览
-
0点赞
-
0收藏
-
0分享
-
154下载
-
0评论
-
引用