刘晨光
1、海洋生物酶的研究与应用;2、多糖生物材料与药物缓释载体;3、酶的固定化与纳米生物技术。
个性化签名
- 姓名:刘晨光
- 目前身份:
- 担任导师情况:
- 学位:
-
学术头衔:
教育部“新世纪优秀人才支持计划”入选者, 博士生导师
- 职称:-
-
学科领域:
生物化学
- 研究兴趣:1、海洋生物酶的研究与应用;2、多糖生物材料与药物缓释载体;3、酶的固定化与纳米生物技术。
刘晨光
教授(博导)
受教育简历:
1986,9-1990,7曲阜师范大学生物学系 获学士学位
1992,9-1995,7四川大学生物学系 获硕士学位
2002,12~2005,8 高丽大学(韩国) 生命科学与技术学院 获博士学位
工作简历:
1990,9~1992,7 济南槐荫中学教师
1995,9~1996,12 中国海洋大学 助教
1997,1~2002,12中国海洋大学 讲师
2003,1~2008,3 中国海洋大学 副教授
2008,4~至今 中国海洋大学 教授(2008年7月获博士生导师资格)
1999,9~2000,2南京大学生物化学系国内访问学者
研究方向:
1、海洋生物酶的研究与应用
2、多糖生物材料与药物缓释载体
3、酶的固定化与纳米生物技术
获奖:
1)教育部提名国家科技进步二等奖(2005年,第3位)
2)山东省高等学校优秀科研成果自然科学奖二等奖(2005年,第3位)
3)青岛市科学技术发明二等奖(2006年,第3位)
4)2008年度中国海洋大学“天泰优秀人才奖”(三等奖)
-
主页访问
1383
-
关注数
0
-
成果阅读
514
-
成果数
10
刘晨光, 李倩, 刘晨光
中国农业科技导报,,2009,11(6):24~29,-0001,():
-1年11月30日
纳米技术是一门研究纳米材料的特性及其应用的新学科,已经迅速发展成为21世纪三大支柱科学领域之一。纳米技术具备促进包括食品行业在内的传统工业快速发展的巨大潜力。综述了纳米技术在食品应用领域中的若干研究热点;着重介绍了其在食品包装材料与营养物运送体系方面的应用,前者包括不可降解性、可生物降解性与可食性纳米复合材料等食品包装材料,后者包括纳米乳剂、脂质体与聚合物纳米粒等营养物运送体系;最后提出了该新领域亟待研究的重要问题和今后的发展方向。
纳米技术, 食品, 纳米粒
-
32浏览
-
0点赞
-
0收藏
-
0分享
-
429下载
-
0评论
-
引用
刘晨光, Yu-long Tan, Chen-Guang Liu∗
Colloids and Surfaces B: Biointerfaces 69(2009)178-182,-0001,():
-1年11月30日
The purpose of the present research work was to study the formation of linoleic acid (LA) modified carboxymethyl chitosan (LCC). Another objectivewas to evaluate effect of linoleic acid degree of substitution on loading capacity (LC), ADR loading efficiency (LE) and in vitro release profile of LCC nanoparticles. The hydrogel nanoparticles can be prepared using linoleic acid modified carboxymethyl chitosan (LACMCS) after the sonication. The critical aggregation concentration (CAC) of the self-aggregate of LA modified CMCS (LCC) was determined by measuring the fluorescence intensity of the pyrene as a fluorescent probe. The CAC values were in the range of 0.061-0.081mg/mL. Self-aggregated nanoparticles exhibited an increased LC and LE, decreased sustained release with an increasing ratio of the hydrophobic LA to hydrophilic CMCS. LCC nanoparticles loaded with ADR exerted in vitro anticancer activity against Hela cells that was comparable to the activity of free (non-entrapped in nanoparticles) ADR.
Chitosan, Linoleic acid, Nanoparticles, Self-aggregates
-
52浏览
-
0点赞
-
0收藏
-
0分享
-
96下载
-
0评论
-
引用
刘晨光, 王艳玲, 郝智慧
中国兽药杂志,2008,42(11):42~46,-0001,():
-1年11月30日
长效制剂由于其可以减少给药次数同时保证药效等一系列优点在兽药领域发展较快。综述了国内外兽药领域开发研制的多种缓控释制剂,包括缓释装置、新型的缓释剂型等。并对其制备、性能药物释放行为做了详尽的说明,并对兽药缓控释制剂未来的发展做了进一步的展望。
兽药, 缓控释制剂, 缓释装置
-
43浏览
-
0点赞
-
0收藏
-
0分享
-
226下载
-
0评论
-
引用
【期刊论文】基于疏水化修饰水溶性多糖的纳米粒制备及应用研究进展*
刘晨光, 董学猛, 金晓明, 刘成圣, 陈西广
动能材科,2007,38:1941~1946,-0001,():
-1年11月30日
疏水化修饰的水溶性聚合物,尤其是多糖,可以通过分子间和分子内的疏水部分之间得交联形成核一壳结构的自聚集纳米粒。纳米粒的制备方法分为超声法与乳化法两种。这类纳米粒是水凝胶基质含有大量水分,在液相中具有静态和动态的稳定性。相对于表面活性剂形成的胶束,多糖自聚集纳米粒在形成过程中具有很低的临界聚集浓度,其疏水微区由多条聚合物链构成并可形成多核结构。综述了以疏水化多糖为基质纳米粒的制备及应用研究状况。疏水化多糖纳米粒能够广泛的结合疏水性药物,有效的将药物靶向运输到病变组织,并保持较长时间的持续释放,提高了疏水药物的生物利用度;它可以通过疏水作用结合蛋白质,提高其抵御变性的能力并提高其热稳性,还可以像“分子伴侣”一样可以预防蛋白质不可逆的变性聚集:同时它也可以通过离子相互作用与DNA结合,保护其不被机体内的各种酶降解,并能以有活性的形式将其释放出来,提高DNA的转染效率。这些结果显示出这类纳米粒在制药及生物技术领域良好的应用前景。
疏水性, 多糖, 纳米粒, 药物运载体系
-
89浏览
-
0点赞
-
0收藏
-
0分享
-
167下载
-
0评论
-
引用
刘晨光, Chenguang Liu a, *, Wenwen Fan a, Xiguang Chen a, Chengsheng Liu a, Xianghong Meng a, Hyun Jin Park b
Current Applied Physics 7S1(2007)e125-e129,-0001,():
-1年11月30日
Linoleic-acid (LA) is covalently conjugated to carboxymethyl-chitosan via a 1-ethyl-3-(3-dimethylaminopropyyl)-carbodiimide-mediated (EDC-mediated) reaction to generate self-aggregated chitosan nanoparticles by sonication. The average diameter of the particles is 417.8 nm, with a size range from 222.8nm to 1028nm. Adriamycin (ADR) can be physically entrapped into the self-aggregates. The drug loading experiments indicate that the loading capacity and efficiency increase with increasing concentration of ADR. ADR is slowly released from chitosan self-aggregates for about 3 days. Furthermore, the effects of drug controlled release become more obvious on increasing the pH value.
Carboxymethyl-chitosan, Linoleic-acid, Nanoparticles, Adriamycin
-
40浏览
-
0点赞
-
0收藏
-
0分享
-
125下载
-
0评论
-
引用
【期刊论文】Preparation and biocompatibility of chitosan microcarriers as biomaterial
刘晨光, Xi-Guang Chena, ∗, Cheng-Sheng Liu a, Chen-Guang Liu a, Xiang-Hong Menga, Chong M. Lee b, Hyun-Jin Park c
Biochemical Engineering Journal 27(2006)269-274,-0001,():
-1年11月30日
Chitosan microcarriers (100-200m) were prepared by the methods of emulsification and ethanol coagulant. It has smooth surface and was stable in phosphate buffer solution (PBS) of at pH 7.2 in the treatment of temperature 120℃ and pressure 150kPa. The chitosan microcarriers showed molecular affinity to the bovine serum proteins at pH 7.2. The adsorptive capacity of the microcarriers to the serum albumin was 6.8mg protein/g chitosan bead. The chitosan microcarriers were found to have good biocompatibility and no cytotoxicity to both human and mouse fibroblasts in tissue cell culture. The fibroblasts well adhered on the smooth surface of the chitosan microcarriers and grew in high cell density. The results suggest a good potential of the chitosan microcarriers as a wound-healing biomaterial.
Affinity, Microcarrier, Tissue cell culture, Adsorption
-
75浏览
-
0点赞
-
0收藏
-
0分享
-
117下载
-
0评论
-
引用
刘晨光, Chen-Guang Liua, b, Xi-Guang Chena, Hyun-Jin Parkb, *
Carbohydrate Polymers 62(2005)293-298,-0001,():
-1年11月30日
The hydrogel nanoparticles can be prepared using linoleic modified chitosan following sonication. The morphology and size distribution of nanoparticles were determined by AFM and photocorrelation spectroscopy, respectively. The effects of environment factors on particle size and adsorption of trypsin (TR) were also analyzed. The LA-chitosan nanoparticles were in the range of 200–600 nm with satisfactory structural integrity. The particle size slightly increased with an increase in concentration. In high ionic strength solution the particle size decreased, compared to salt-free solution, on the other hand, the particles size increases as urea concentration increased. The particle size was found to be larger in acidic solution than in neutral and alkaline solution. Environment factors (e.g. pH, concentration of urea or NaCl) can affect TR loading on the nanoparticle. The thermal stability of TR loading on nanoparticles was significantly improved compared to free TR.
Hydrophobically modified chitosan, Nanoparticles, Trypsin, Adsorption
-
47浏览
-
0点赞
-
0收藏
-
0分享
-
192下载
-
0评论
-
引用
刘晨光, CHEN-GUANG LIU, †, § KASHAPPA GOUD H. DESAI, § XI-GUANG CHEN, † AND HYUN-JIN PARK*, §
J. Agric. Food Chem. 2005, 53, 1728-1733,-0001,():
-1年11月30日
Trypsin was immobilized on linolenic acid modified chitosan using glutaraldehyde (GA) as crosslinker, which was confirmed by Fourier transform infrared (FTIR) spectra. The chitosan nanoparticles containing trypsin (TR) can be prepared after the sonication of immobilized trypsin. The GA concentration affected both the enzyme activity of the nanoparticle and particle size. Results indicated that the activity of trypsin immobilized onto linolenic acid modified chitosan nanoparticles increased with increasing concentration of GA up to 0.07% (v/v) and then decreased with increasing amount of GA. On the other hand, particle size increased (from 523 to 1372 nm) with the increasing concentration of GA (from 0.03 to 0.1% v/v). The enzyme catalytic characteristics of nanoparticle solution were also studied. The results showed that the kinetic constant value (Km) of TR immobilized on nanoparticle (71.9mg/mL) was higher than that of pure TR (50.2mg/mL). However, the thermal stability and optimum temperature of TR immobilized on nanoparticles improved, which make it more attractive in the application aspect.
Chitosan, nanoparticles, trypsin, hydrophobically modified chitosan
-
39浏览
-
0点赞
-
0收藏
-
0分享
-
79下载
-
0评论
-
引用
刘晨光, Chenguang Liu, Kashappa Goud H. Desai, * and Chengsheng Liu
J. Chem. Eng. Data 2005, 50, 278-282,-0001,():
-1年11月30日
The present study investigated the solubilization of valdecoxib in aqueous solution using poly(ethylene glycol) 4000, poly(ethylene glycol) 6000, poly(ethylene glycol) 8000, and poly(ethylene glycol) 10 000 at (298.15, 303.15, and 308.15) K. The analysis of valdecoxib is carried out by UV spectral measurements at ìmax ) 202 nm. The aqueous solubility of valdecoxib could be enhanced by the addition of an increasing mass fraction of all of the poly(ethylene glycols) tested as well as by increasing the temperature of the dissolution media. The molecular weight of the poly(ethylene glycols) tested played an important role in valdecoxib solubilization in the aqueous medium. Among the poly(ethylene glycols) studied, poly(ethylene glycol) 4000 exhibited a higher solubilization potential than the others. Calculated Gibbs free energy values were all negative for all of the poly(ethylene glycol) + water mixtures at (298.15, 303.15, and 308.15) K, indicating the spontaneous nature of valdecoxib solubilization. In the case of poly(ethylene glycol) 4000 + water mixtures, the ¢trG° values decreased to a greater extent than those for the other carriers {poly(ethylene glycol) 6000, poly(ethylene glycol) 8000, and poly(ethylene glycol) 10 000) + water mixtures}, indicating that the reaction conditions were more favorable in poly(ethylene glycol) 4000+water mixtures than in other carrier + water mixtures.
-
53浏览
-
0点赞
-
0收藏
-
0分享
-
139下载
-
0评论
-
引用
【期刊论文】Linolenic Acid-Modified Chitosan for Formation of Self-Assembled Nanoparticles
刘晨光, CHEN-GUANG LIU, †, ‡ KASHAPPA GOUD H. DESAI, ‡ XI-GUANG CHEN, † AND HYUN-JIN PARK*, ‡
,-0001,():
-1年11月30日
Chitosan was modified by coupling with linolenic acid through the 1-ethyl-3-(3-dimethylaminopropyyl)-carbodiimide-mediated reaction. The degree of substitution was measured by 1H NMR, and it was 1.8%, i.e., 1.8 linolenic acids group per 100 anhydroglucose units. The critical aggregation concentration (CAC) of the self-aggregate of hydrophobically modified chitosan was determined by measuring the fluorescence intensity of the pyrene as a fluorescent probe. The CAC value in phosphate-buffered saline (PBS) solution (pH 7.4) was 5 10-2mg/mL. The average particle size of self-aggregates of hydrophobically modified chitosan in PBS solution (pH 7.4) was 210.8 nm with a unimodal size distribution ranging from 100 to 500nm. A transmission electron microscopy study showed that the formation of near spherical shape nanoparticles had enough structural integrity. The loading ability of hydrophobically modified chitosan (LA-chitosan) was investigated by using bovine serum albumin (BSA) as a model protein. Self-aggregated nanoparticles exhibited an increased loading capacity (19.85 (0.04 to 37.57 (0.25%) with an increasing concentration of BSA (0.1-0.5mg/ mL)
Chitosan, linolenic acid, nanoparticles, self-aggregates, BSA
-
44浏览
-
0点赞
-
0收藏
-
0分享
-
76下载
-
0评论
-
引用