王雪梅
研究方向包括分子有序结构的组装与表征、分子/纳米器件的研究、生物纳米材料及其应用、生物电活性器件、生物传感器和微阵列芯片。
个性化签名
- 姓名:王雪梅
- 目前身份:
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学术头衔:
博士生导师
- 职称:-
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学科领域:
生物化学
- 研究兴趣:研究方向包括分子有序结构的组装与表征、分子/纳米器件的研究、生物纳米材料及其应用、生物电活性器件、生物传感器和微阵列芯片。
王雪梅,女,教授,博士生导师。
1968年生, 1995年获南京大学博士学位,后留校工作。1996年6月获洪堡基金赴德国从事生物超分子识别等方面的研究工作,1998年2月被东南大学生物科学与医学工程系聘为教授、博士生导师。其后分别为香港浸会大学(2001),以色列希伯莱大学(2002),德国萨尔州大学(2004),和美国克拉克森大学(2008)等高级访问学者和访问教授。2000年获香港裘槎研究基金和中国化学会青年化学奖。2005年入选教育部“新世纪优秀人才支持计划”。2007年获“中国青年女科学家奖”。
研究方向包括分子有序结构的组装与表征、分子/纳米器件的研究、生物纳米材料及其应用、生物电活性器件、生物传感器和微阵列芯片。利用交叉学科的新成果,研究分子探针与生物大分子或细胞间的识别作用,设计分子识别的模型系统、生物功能探针和诊治新技术,构建仿生界面和集纳米生物技术与高灵敏检测于一体的智能化分子在线检测新方法,发展无创疾病分子诊治新技术,并阐明某些肿瘤与耐药性的分子机制,为基因靶标药物的设计、肿瘤的筛查、早期诊治提供新思路与新方法。
近五年主持部省级以上科研项目10余项,发表SCI论文80余篇,应邀主编英文专著1部,英文专章3章、中文专章1章,申请专利多项。
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主页访问
1207
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成果阅读
863
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成果数
19
王雪梅, Min Song a, Renyun Zhang a, Yongyuan Dai b, Feng Gao b, Huimei Chi a, Gang Lv a, Baoan Chen b, Xuemei Wang a, *
Biomaterials 27(2006)4230-4238,-0001,():
-1年11月30日
The appearance of drug-resistant (especially, multidrug-resistant (MDR)) tumor cells is a major obstacle to the success of chemotherapy; thus, the development of effective anti-MDR agents plays an important role in the tumor therapy. In this report, the considerable effect of nano-TiO2 and UV illumination on the drug resistance of target cancer cells has been explored, and the fresh evidence from the fluorescence spectroscopy and microscopy as well as electrochemical studies demonstrates the significant enhancement effect of nano-TiO2 to the drug uptake by drug-resistant leukemia cells. Besides, it is also observed that the combination of the nano-TiO2 and UV irradiation with the accompanying anticancer drug daunorubicin could provoke some considerable changes of the cell membrane of the target leukemia cells, which indicates that nano-TiO2 could not only increase the drug accumulation in target cancer cells, but also act as an effective anti-MDR agent to inhibit the relative drug resistance.
Drug delivery, Electrochemistry, Confocal fluorescence, Titanium oxide, Drug resistance, Leukemia
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王雪梅, Xuemei Wang, Andrei B. Kharitonov, Eugenii Katz and Itamar Willner*
CHEM. COMMUN., 2003, 1542-1543,-0001,():
-1年11月30日
A non-dense long-chain tethered bipyridinium monolayer linked to an electrode support is reversibly retracted and attracted from and to the electrode respectively by the applied potential. The "molecular machinery" functions of the monolayer are probed by chronoamperometry and contact angle measurements.
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王雪梅, Renyun Zhang, XuemeiWang, , ChunhuiWu, Min Song, Jingyuan Li, Gang Lv, Jian Zhou, Chen Chen, Yongyuan Dai, Feng Gao, Degang Fu, Xiaomao Li, Zhiqun Guan and Baoan Chen
Nanotechnology 17(2006)3622-3626,-0001,():
-1年11月30日
Three kinds of magnetic nanoparticle, tetraheptylammonium capped nanoparticles of Fe3O4, Fe2O3 and Ni have been synthesized, and the synergistic effect of these nanoparticles on the drug accumulation of the anticancer drug daunorubicin in leukaemia cells has been explored. Our observations indicate that the enhancement effect of Fe3O4 nanoparticles is much stronger than that of Fe2O3 and Ni nanoparticles, suggesting that nanoparticle surface chemistry and size as well as the unique properties of the magnetic nanoparticles themselves may contribute to the synergistic enhanced effect of the drug uptake of targeted cancer cells.
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王雪梅, Xuemei Wang, Eugenii Katz, Itamar Willner*
Electrochemistry Communications 5(2003)814-818,-0001,():
-1年11月30日
Asymmetrical deformation of an aqueous electrolyte droplet bridging two bipyridinium-modified Au-electrodes is achieved upon the application of voltages resulting in the selective reduction and oxidation of the bipyridinium units on the two electrodes. The different contact angles at the right and left sides of the droplet originate from the electrochemically controlled changes of the wetting properties of the modified electrodes. The reversible and repeatable formation of an electrolytic contact between a side Ptwire electrode and the respective bottom Au-electrode is controlled by the voltage applied between the two bottom Au-electrodes.
Contact angle, Bipyridinium electrochemistry, Electrochemical switch, Monolayer, Modified electrode
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【期刊论文】In situ electrochemical contact angle study of hemoglobin and hemoglobin–Fe3O4 nanocomposites
王雪梅, Renyun Zhang, Min Song, Xiaomao Li, Zhiqun Guan, Xuemei Wang
Anal Bioanal Chem (2006) 386: 2075-2079,-0001,():
-1年11月30日
The electrochemical redox-induced contact angle changes of hemoglobin droplets in the absence and presence of tetraheptylammonium-capped Fe3O4 nanoparticles have been explored by using in situ electrochemical contact angle measurements. The results indicate that the electrochemical redox process may lead to some structure changes of hemoglobin (Hb), which could further induce the hydrophobic-to-hydrophilic changes of the relative droplets. Our observations demonstrate that hemoglobin could self-assemble on the surface of the functionalized Fe3O4 nanoparticles as Hb–Fe3O4 nanocomposites, which may contribute to much more significant change of the electrochemical redox-induced contact angle values than that with free nano-Fe3O4. These results suggest that in situ electrochemical contact angle measurements could be readily applied as a new and convenient method to detect some specific biological process.
Biomaterials, Nanoparticles, Bioanalytical methods, Electroanalytical methods, UV/, Vis
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王雪梅, Jingyuan Li, a, b Chunhui Wu, a Feng Gao, c Renyun Zhang, a Gang Lv, a Degang Fu, a Baoan Chenc and Xuemei Wanga, b, *
Bioorganic & Medicinal Chemistry Letters 16(2006)4808-4812,-0001,():
-1年11月30日
We report a novel approach to enhance the efficient accumulation and utilization of anticancer drug daunorubicin on cancer cells through the combination with CdS nanoparticles. Our observations using confocal fluorescence scanning microscopy as well as electrochemical analysis methods demonstrate that CdS nanoparticles can readily bind with daunorubicin on the external membrane of the targeted cells and facilitate the uptake of drug molecules in the human leukemia K562 cells. Besides, our results also indicate that the competitive binding of CdS nanoparticles with accompanying anticancer drug to the membrane of leukemia K562 cells could efficiently prevent the drug release by the drug-sensitive and drug-resistant leukemia cells and thus inhibit the possible multidrug resistance of cancer cells, which could be further utilized to improve the future drug efficiency in respective tumor chemotherapies.
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王雪梅, Min Song, Renyun Zhang, Xuemei Wang*
Materials Letters 60(2006)2143-2147,-0001,():
-1年11月30日
The biomolecular binding behavior of anticancer drug dacarbazine (DTIC) in the presence of titanium dioxide (TiO2) nanoparticle interface has been studied by using electrochemical methods. The results indicate that the presence of TiO2 nanoparticles can obviously increase the binding affinity of DTIC to DNA or DNA bases and significantly enhance the detection sensitivity for the relative biomolecular recognition.
Titanium dioxide, Nanomaterials, Electrochemistry, Biomolecular recognition, DNA
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【期刊论文】光谱法研究亚甲基绿(MG)、亚甲基蓝(MB)与DNA的识别作用及纳米材料对识别作用的影响
王雪梅, 张仁云, 王雪梅*, 平刚, 李尧
中国生物医学工程学报,2006,25(6):776~779,-0001,():
-1年11月30日
通过可见光谱法研究了亚甲基绿(MG)、亚甲基蓝(MB)与cT-DNA的相互作用,并且研究了纳米材料胶体金对该识别作用的影响。研究结果表明,MG、MB与DNA相互作用方式是不相同的,尽管它们在化学结构上只存在很小的差异。MG是以插入方式与DNA作用的,而MB与DNA作用存在沟槽和插入两种方式,并以沟槽方式为主。此外,通过在胶体金溶液中的研究表明,胶体金可以明显地提高小分子化合物与DNA作用检测的灵敏度。
光谱分析, DNA识别, 亚甲基绿, 亚甲基蓝, 胶体金
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王雪梅, Ren-Yun Zhang, Xue-Mei Wang*, Sheng-Jin Gong, and Nong-Yue He
Geno. Prot. Bioinfo. Vol. 3 No.1 2005,-0001,():
-1年11月30日
The study of small drug molecules interacting with nucleic acids is an area of intense research that has particular relevance in our understanding of relative mechanism in chemotherapeutic applications and the association between genetics (including sequence variation) and drug response. In this contribution, we demonstrate how the sequence-specic binding of an anticancer drug Dacarbazine (DTIC) to single base (A-G) mismatch could be sensitively detected by combining electrochemical detection with biosensing surface based on gold nanoparticles.
single base mismatch,, DTIC,, electrochemistry,, gold nanoparticles,, biosensor
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王雪梅, Xuemei Wang, Shiri Zeevi, Andrei B. Kharitonov, Eugenii Katz and Itamar Willner*
Phys. Chem. Chem. Phys., 2003, 5, 4236-4241,-0001,():
-1年11月30日
In situ electrochemical static contact angle measurements are employed to probe photoinduced electron transfer processes at electrode surfaces. Photosystems consisting of tris(2,20-bipyridyl)ruthenium(II), (Ru(bpy)32+), N,N0-dimethyl-4,40-bipyridinium (MV2+), Na2EDTA or CdS nanoparticles, MV2+, triethanolamine (TEOA) were included in aqueous droplets that were coupled with a ferrocene monolayer-functionalized electrode. Upon the oxidation of the interface (0.5 V vs. Ag quasi-reference electrode), and the illumination of the systems, photoinduced electron transfer to the modified electrode proceeds. The electron transfer processes yield photocurrents, and the photoelectrochemical transformations are followed by contact angle measurements. Similarly, a CdS/bipyridinium monolayer is assembled on an Au electrode support. The redox transformations of the bipyridinium units control the hydrophilic/hydrophobic properties of the interface, and are followed by contact angle analyses. Irradiation of the CdS/bipyridinium layer associated with the electrode biased at -0.3 V yields a photocurrent. The changes in the wetting properties of the irradiated interface are investigated by contact angle measurements.
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