印春华
(1)生物大分子药物与给药系统研究;(2)功能性高分子材料与分子药剂学研究;(3)中草药有效成分与化学研究;(4)新药及药物新剂型的研究与开发。
个性化签名
- 姓名:印春华
- 目前身份:
- 担任导师情况:
- 学位:
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学术头衔:
博士生导师
- 职称:-
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学科领域:
药剂学
- 研究兴趣:(1)生物大分子药物与给药系统研究;(2)功能性高分子材料与分子药剂学研究;(3)中草药有效成分与化学研究;(4)新药及药物新剂型的研究与开发。
印春华
简历:
1986年7月毕业于中国药科大学药学系,获学士学位;
1986年7月至1988年8月在武汉第四制药厂任助理工程师;
1993年5月毕业于中国药科大学药剂学专业,获博士学位;
1993年6月至1994年8月在南京国科公司任高级工程师;
1996年8月从军事医学科学院博士后出站;
1996年8月起任复旦大学生命科学学院遗传所生物技术中心副教授;
2000年12月起任教授。现为复旦大学生命科学学院教授,博士生导师;
复旦大学药学院兼职教授,药剂学专业博士生导师。
国际控释学会(CRS)会员,中国药学会高级会员。
1998年5月起担任中国药学杂志编委,2002年10月聘为国家药典委员会委员。
主要研究方向:
(1)生物大分子药物与给药系统研究;
(2)功能性高分子材料与分子药剂学研究;
(3)中草药有效成分与化学研究;
(4)新药及药物新剂型的研究与开发。
获奖情况:
作为研究负责人和主要研究者,已获国家批准的新药证书20余项;
(2)申请中国发明专利7项,已获批准的发明专利3项;
(3)近年来发表SCI等论文十多篇;
(4)获2003年教育部科学技术进步奖一等奖(第一完成人);2003年上海市科学技术进步奖二等奖(第一完成人);2003年上海市优秀发明选拔赛一等奖(第一完成人)。
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1339
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400
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成果数
7
印春华, Lichen Yin a, JieYing Ding b, Likun Fei a, Miao Heb, Fuying Cui b, Cui Tang a, ??, Chunhua Yin a, b, ?
International Journal of Pharmaceutics 350(2008)220-229,-0001,():
-1年11月30日
In this investigation, superporous hydrogels containing poly (acrylic acid-co-acrylamide)/O-carboxymethyl chitosan (O-CMC) fullinterpenetrating polymer networks (SPH-IPNs) were evaluated for their potentials in effective insulin absorption via the oral route. Insulin release from the SPH-IPNs exhibited sensitivity towards pH and ionic strength. After drug loading and release, the circular dichroism (CD) spectra revealed that conformation of insulin had no significant alteration and bioactivity of insulin was well preserved according to hypoglycaemic effect in mice. Through their abilities to bind Ca2+ and to entrap the enzymes, SPH-IPNs could partly inactivate trypsin and-chymotrypsin, and SPH-IPN with higher O-CMC/monomer ratio appeared more potent. Swollen SPH-IPNs could attach mechanically and muco-adhere to the intestinal wall, thus achieving improved retentive properties compared to commonly used muco-adhesive excipient Carbopol? 934. Transport of insulin across rat intestine and colon ex vivo was enhanced around two- to three-fold after application of the SPH-IPN. Insulin-loaded SPH-IPN showed significant hypoglycaemic effects following oral administration to healthy rats, achieving a 4.1% pharmacological availability compared to subcutaneous insulin injection. These pronounced properties demonstrated that the SPH-IPN would be a promising peroral carrier for insulin and other peptide drugs.
Poly (, butylcyanoacrylate), , Poly (, ethylene glycol), , Paclitaxel, Nanocapsules
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印春华, Yu Zhang a, b, Siyu Zhu a, Lichen Yin a, Feng Qian a, Cui Tang a, Chunhua Yin a, *
European Polymer Journal 44(2008)1654-1661,-0001,():
-1年11月30日
A new type of nanocapsules with an oil core, coated by poly(ethylene glycol) (PEG) was designed. The loading efficiency and the biocompatibility of the polymeric nanocapsules were evaluated when it was used as a carrier for hydrophobic agent paclitaxel. The nanocapsules were synthesized through miniemulsion polymerization of butylcyanoacrylate (BCA) with PEG as initiator. The particle size and zeta potential of nanocapsules were influenced by the PEG content in the polymerization system. Fourier transform infrared (FTIR) spectra and 1H NMR demonstrated the chemical coupling between PEG and poly(butylcyanoacrylate) (PBCA). Thermal characteristics of the copolymer were investigated by differential scanning calorimetry (DSC). The encapsulation efficiency increased concurrently with the increase of the PEG content in the system. The hemolytic assay and the cytotoxicity measurement showed that the PEG coating could significantly reduce the hemolytic potential and cytotoxicity of the nanocapsules. The results showed that the PEG-PBCA nanocapsules could be an effective carrier for hydrophobic agents.
Poly (, butylcyanoacrylate), , Poly (, ethylene glycol), , Paclitaxel, Nanocapsules
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印春华, Chunbai He, Fuying Cui, Lichen Yin, Feng Qian, Cui Tang, Chunhua Yin *
European Polymer Journal 45(2009)368-376,-0001,():
-1年11月30日
A smart polymeric composite carrier consisting of carboxylated chitosan grafted nanoparticles (CCGN) and bilaminated films with one alginate-Ca2+ mucoadhesive layer and one hydrophobic backing layer was developed as a novel carrier for peptide. Calcein, hydrophilic and hydrolytic degradative, was entrapped into CCGN as a model peptide and its release behavior was investigated. Morphology study showed a uniform distribution of CCGN in the homogeneous and porous hydrogel. CCGN was characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), particle size measurement, and f potential measurement. The composite carrier was characterized by differential scanning calorimetry (DSC), scanning electron microscope (SEM) and fluorescence microscopy. The carrier exhibited high mucoadhesive force and pH-sensitivity, in that release of the nanoparticles and the model peptide calcein were both restricted in acidic environment while a fast and complete release was achieved in neutral medium. Therefore, this novel carrier would be a promising candidate for hydrophilic peptide drugs via oral administration.
Nanoparticles, Bilaminated films, pH-sensitivity, Mucoadhesive force, Calcein
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印春华, Lichen Yin, Likun Fei, Fuying Cui, Cui Tang*, Chunhua Yin**
Biomaterials 28(2007)1258-1266,-0001,():
-1年11月30日
Superporous hydrogels containing poly(acrylic acid-co-acrylamide)/O-carboxymethyl chitosan interpenetrating polymer networks (SPH-IPNs) were prepared by cross-linking O-carboxymethyl chitosan (O-CMC) with glutaraldehyde (GA) after superporous hydrogel (SPH) was synthesized. The structures of the SPH-IPNs were characterized with FT-IR, 13C-NMR and DSC. SEM, CLSM and light images revealed that the SPH-IPNs possessed both the IPN network and large numbers of pores and the cross-linked O-CMC molecules were located on the peripheries of these pores. The swelling behavior of SPH-IPNs was dependent on the O-CMC content, GA amount and cross-linking time. Due to the cross-linked O-CMC network, in vitro muco-adhesive force and mechanical properties, including compression and tensile modulus, of the SPH-IPN were greatly improved when compared with the CSPH. An enhanced loading capacity for insulin could be obtained by the SPH-IPNs as compared to non-porous hydrogel and CSPH, and more than 90% of the insulin was released within 1h. With the improved mechanical properties, in vitro muco-adhesive force and loading capacities, the SPH-IPN may be used as a potential muco-adhesive system for peroral delivery of peptide and protein drugs.
O-carboxymethyl chitosan, Interpenetrating polymer network hydrogel, Porosity, Mechanical properties, Mucosal adhesion, Drug delivery
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印春华, Siyu Zhu a, b, , Feng Qian a, Yu Zhang a, Cui Tang a, Chunhua Yin a, *
European Polymer Journal 43(2007)2244-2253,-0001,():
-1年11月30日
Chitosan-N-trimethylaminoethylmethacrylate chloride-PEG (CS-TM-PEG) copolymers were synthesized in order to improve the solubility of chitosan in physiological environment, and enhance the biocompatibility of quaternized chitosan. The result of 1H NMR confirmed that PEG had been combined with amino groups of quaternized chitosan. The profile of hemolysis assay showed that Chitosan-N-trimethylaminoethylmethacrylate chloride (CS-TM) copolymer exhibited hemolytic activity from 10.31% to 13.58%, while CS-TM-PEG copolymer had hemolytic activity from 4.76% to 7.05% at copolymer concentrations from 250 to 2000 lg/ml. Through PEG modification, the hemolytic activity could be reduced to a half. CS-TM-PEG copolymer-insulin nanoparticles were prepared based on ionic gelation process of positively charged copolymers and negatively charged insulin. The nanoparticles were characterized in terms of particle size, TEM, association efficiency and in vitro release. These nanoparticles were 200-400 nm in size and insulin association efficiency of optimal formulations was found up to 90%. In vitro release showed that the nanoparticles provided an initial burst release followed by a sustained release with the sensitivity of ionic strength and pH values.
Quaternized chitosan, PEG modification, Insulin, Nanoparticles, Hemolysis
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【期刊论文】Preparation and characterization of mucoadhesive polymer-coated nanoparticles
印春华, Fuying Cui a, b, Feng Qian a, Chunhua Yin a, ?
International Journal of Pharmaceutics 316(2006)154-161,-0001,():
-1年11月30日
The transmucosal routes such as pulmonary, nasal and oral routes are most important and common routes for drug delivering to the body. However, peptide and protein drugs are degraded before they reach the blood stream and cannot cross the mucosal barriers. The mucoadhesive polymer-coated nanoparticles colloidal carriers can solve these problems. In the present investigation, mucoadhesive polymer-coated nanoparticles were prepared by emulsion polymerization process. A detailed preparation procedure of the mucoadhesive polymer-coated nanoparticles was provided. The parameters such as portion of the mucoadhesive polymers and concentration of the radical initiator were investigated. The resulting chitosan-coated nanoparticles colloids possessed positive surface charge, while poly(acrylic acid)-coated nanoparticles colloids and carbopol-coated nanoparticles colloids had negative surface charge. These nanoparticles were suitable for carrying hydrophilic protein or peptide drugs. Chitosancoated nanoparticles were stable when pH value below 11, while poly(acrylic acid)-coated nanoparticles and carbopol-coated nanoparticles were stable under physiological pH conditions. Therefore, they are promising for transmucosal drug delivery.
Nanoparticles, Mucoadhesive polymer-coated nanoparticles, Emulsion polymerization, Surface charge, Drug delivery
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印春华, Feng Qian a, b, Fuying Cui a, Chunhua Yin a, *
European Polymer Journal 42(2006)1653-1661,-0001,():
-1年11月30日
Methylmethacrylate copolymer nanoparticles with diVerent hydrophilic chains were prepared by the free radical polymerization of methylmethacrylate with N-isopropylacrylamide (NIPAAm), N-methacrylic acid (MAA), N-trimethylaminoethylmethacrylate chloride (TMAEMC) or N-dimethylaminoethylmethacrylate hydrochloride (DMAEMC). These particles were characterized by particle size and zeta potential. The polymerization conditions were shown to inXuence the particle size and surface charge. Particle sizes of MMA-NIPAAm nanoparticles after 3h of reaction reached constant level at 180 nm. An increasing amount of total monomer (0.5-5%) would result in the nanoparticles of particle size of 115-204nm for 30% NIPAAm of the total monomer. In the same range of 5-40% NIPAAm of the total monomer, the particle size decreased from 280 to 170nm. The concentration of the initiator APS up to a concentration of 0.2% for MMA-TMAEMC and 0.1% for MMA-NIPAAm showed no eVect on the particle size of the Wnal nanoparticle suspensions, while higher concentration would lead to aggregation in the polymerization process. MMA-NIPAAm nanoparticles were pH-dependent in zeta potential at pH 1-12 values reducing from 12.2mV to ?16.8mV, respectively. Nanoparticles were incubated with pepsin and trypsin at 37℃ for 20min and their enzyme inhibition was determined. The activity of pepsin decreased to 27% in the presence of MMA-NIPAAm nanoparticles, and MMA-MAA nanoparticles reduced the activity of trypsin to 39%, respectively.
Methylmethacrylate copolymer, Nanoparticles, Enzyme inhibition
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