尚忠林
主要从事植物发育生理方面的研究,重点是植物细胞信号转导机制。
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- 姓名:尚忠林
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学术头衔:
博士生导师
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学科领域:
植物学
- 研究兴趣:主要从事植物发育生理方面的研究,重点是植物细胞信号转导机制。
尚忠林、博士、教授、硕士生导师、生命科学学院科研副院长1970年出生,河北泊头人。1988年考入河北师范大学生物系生物教育专业读本科;1992年考取本校植物生理学专业硕士研究生;1995年毕业后留校任教,1998-2001年在中国农业大学生物学院攻读博士学位,2001-2003年在河北师范大学生物学博士后流动站从事博士后科研工作。1992年加入中国共产党。1997年评定讲师、2002年评定副教授职称,现为生命科学学院教授、教工第二党支部书记。
参加工作以来承担本科生植物生理学、植物生物学、植物发育生理学等课程的教学工作,曾经主持河北师大教务处教学改革课题1项。
近年来主要从事植物发育生理方面的研究,重点是植物细胞信号转导机制。实验室目前拥有植物生理学、植物生物化学和植物细胞生物学实验设备十几台(套),总价值超过150万,可以开展植物生理学、植物细胞生物学以及植物分子生物学相关研究。目前主持国家自然科学基金课题一项、河北省自然科学基金课题一项、河北省教育厅博士基金课题一项以及河北师范大学博士基金课题一项。1998年获得河北省教育厅科技进步三等奖一次。截至2005年3月已经在国内外植物学杂志上发表论文20多篇。目前指导硕士研究生6名,利用实验室的膜片钳记录系统、荧光比率显微测定系统等大型精密仪器,开展植物细胞内钙离子信号的生理作用、钙信号产生机制、细胞质膜钙通道在钙信号产生过程中的作用及其调控机制等课题的研究。
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【期刊论文】Calmodulin Is Involved in Heat Shock Signal Transduction in Wheat1
尚忠林, Hong-Tao Liu, Bing Li, Zhong-Lin Shang, Xiao-Zhi Li, Rui-Ling Mu, Da-Ye Sun, and Ren-Gang Zhou*
Plant Physiology, July 2003, Vol.132, pp.1186-1195,-0001,():
-1年11月30日
The involvement of calcium and calcium-activated calmodulin (Ca2-CaM) in heat shock (HS) signal transduction in wheat (Triticum aestivum) was investigated. Using Fluo-3/acetoxymethyl esters and laser scanning confocal microscopy, it was found that the increase of intracellular free calcium ion concentration started within 1 min after a 37℃ HS. The levels of CaM mRNA and protein increased during HS at 37℃ in the presence of Ca2. The expression of hsp26 and hsp70 genes was up-regulated by the addition of CaCl2 and down-regulated by the calcium ion chelator EGTA, the calcium ion channel blockers LaCl3 and verapamil, or the CaM antagonists N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide and chlorpromazine. Treatment with Ca2+ also increased, and with EGTA, verapamil, chlorpromazine, or trifluoperazine decreased, synthesis of HS proteins. The temporal expression of the CaM1-2 gene and the hsp26 and hsp70 genes demonstrated that up-regulation of the CaM1-2 gene occurred at 10 min after HS at 37℃, whereas that of hsp26 and hsp70 appeared at 20 min after HS. A 5-min HS induced expression of hsp26 after a period of recovery at 22℃ after HS at 37℃. Taken together, these results indicate that Ca2-CaM is directly involved in the HS signal transduction pathway. A working hypothesis about the relationship between upstream and downstream of HS signal transduction is presented.
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尚忠林, Hai-Yong Qu, , Zhong-Lin Shang, Shao-Ling Zhang, Lian-Mei Liu and Ju-You Wu
New Phytologist (2007) 174: 524-536,-0001,():
-1年11月30日
The pollen tube has been widely used to study the mechanisms underlying polarized tip growth in plants. A steep tip-to-base gradient of free cytosolic calcium ([Ca2+]cyt) is essential for pollen-tube growth. Local Ca2+ influx mediated by Ca2+-permeable channels plays a key role in maintaining this [Ca2+]cyt gradient. Here, we developed a protocol for successful isolation of spheroplasts from pollen tubes of Pyrus pyrifolia and identified a hyperpolarization-activated cation channel using the patch-clamp technique. We showed that the cation channel conductance displayed a strong selectivity for divalent cations, with a relative permeability sequence of barium (Ba2+)≈Ca2+>magnesium (Mg2+)> strontium (Sr2+)>manganese (Mn2+). This channel conductance was selective for Ca2+ over chlorine (Cl-) (relative permeability PCa/PCl=14 in 10 mM extracellular Ca2+). We also showed that the channel was inhibited by the Ca2+ channel blockers lanthanum (La3+) and gadolinium (Gd3+). Furthermore, channel activity depended on extracellular pH and pollen viability. We propose that the Ca2+-permeable channel is likely to play a role in mediating Ca2+ influx into the growing pollen tubes to maintain the [Ca2+]cyt gradient.
Ca2+, -permeable channels,, patch clamp,, pollen tube spheroplasts,, Pyrus pyrifolia.,
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尚忠林, Yansheng Wu, Xiaodong Xu, Sujuan Li, Ting Liu, Ligeng Ma and Zhonglin Shang
New Phytologist (2007) 176: 550-559,-0001,():
-1年11月30日
The role of heterotrimeric G proteins in pollen germination and tube growth was investigated using Arabidopsis thaliana plants in which the gene (GPA) encoding the G-protein α subunit (G α) was null or overexpressed. Pollen germination, free cytosolic calcium concentration ([Ca2+ ] cyt) and Ca2+ channel activity in the plasma membrane (PM) of pollen cells were investigated. Results showed that, compared with pollen grains of the wild type (ecotype Wassilewskija, ws), in vitro germinated pollen of G α null mutants (gpa1-1 and gpa1-2) had lower germination percentages and shorter pollen tubes, while pollen from G α overexpression lines (wG α and cG α) had higher germination percentages and longer pollen tubes. Compared with ws pollen cells, [Ca2+] cyt was lower in gpa1-1 and gpa1-2 and higher in wG α and cG α. In whole-cell patch clamp recordings, a hyperpolarization-activated Ca2+-permeable conductance was identified in the PM of pollen protoplasts. The conductance was suppressed by trivalent cations but insensitive to organic blockers; its permeability to divalent cations was Ba2+>Ca2+>Mg2+>Sr2+>Mn2+. The activity of the Ca2+-permeable channel conductance was down-regulated in pollen protoplasts of gpa1-1 and gpa1-2, and up-regulated in wG α and cG α. The results suggest that G α may participate in pollen germination through modulation of the hyperpolarization-activated Ca2+ channel in the PM of pollen cells.
Arabidopsis thaliana,, heterotrimeric G protein,, hyperpolarizationactivated calcium channel,, patch clamp,, pollen
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