王傲雪
植物分子细胞生物学
个性化签名
- 姓名:王傲雪
- 目前身份:
- 担任导师情况:
- 学位:
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学术头衔:
博士生导师
- 职称:-
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学科领域:
生物化学
- 研究兴趣:植物分子细胞生物学
王傲雪
植物学博士后 生物化学博士后 农学博士
学习和工作经历:
2005.3-现在 东北农业大学园艺学院教授、博士生导师
2002.4-2005.3 植物学博士后,加拿大圭尔夫大学生物科学学院分子细胞生物学系
1999.9-2001.9 生物化学博士后,南京大学生物化学系医药生物技术国家重点实验室
1996.9-2001.7 博士研究生,东北农业大学园艺系
1994.9-1996.7 硕士研究生,东北农业大学园艺系
1990.9-1194.7 本科,东北农业大学园艺系
研究方向:
植物分子细胞生物学
科研简述:
1、阐明番茄生长发育中重要性状的分子生物学机理,通过植物发育的分子机理方面的研究克隆出控制植物发育的关键基因,从而利用这些基因来改良园艺作物的重要性状。
2、研究植物和微生物中的细胞壁降解酶功能,从而阐明细胞壁降解酶在植物生长发育中的角色。
3、通过植物作为反应器来表达医药蛋白,达到获得高效表达医药蛋白的转基因植物。
社会兼职:
加拿大植物生理学会(CSPP) 会员
国际种子学会(ISSS) 会员
黑龙江省园艺学会 常务理事
黑龙江省遗传学会 理事
中国统计学会农村统计分会 理事
获得奖励情况:
项目“番茄高效育种技术及优质、多抗、丰产系列专用新品种选育与推广”2005年获得国家科学技术进步奖二等奖(证书号:2005-J-201-2-07-R09)
项目“优质多抗丰产加工专用型番茄新品种东农706的育成及推广”:2007年度黑龙江省省长特别奖(证书号:2007-33)
目前承担科研项目情况:
1、国家自然科学基金项目 “甘露聚糖酶参与番茄果实发育与成熟的分子调控研究”(NSFC No.30600414),国家自然科学基金委员会,2007.1-2009.12,主持人
2、教育部留学回国人员科研启动基金“甘露聚糖酶在番茄果实发育中的表达与作用”2007.1-2009.12,国家教育部,主持人
3、黑龙江省科技攻关项目“类番茄茄中抗冷转录因子CBF基因的克隆与功能分析”,2006.1-2008.12,黑龙江省科技厅,主持人
4、哈尔滨市科技局留学归国人员基金项目“新型灰霉病生防酵母菌菌种的构建”,2006.1-2008.12,哈尔滨市科委,主持人
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成果数
6
王傲雪, XUEMEI GONG, GEORGE W. BASSEL, AOXUE WANG, JOHN S. GREENWOOD and J. DEREK BEWLEY*
Annals of Botany 96: 1165-1173, 2005,-0001,():
-1年11月30日
Background and Aims Seeds of carob, Chinese senna, date and fenugreek are hard due to thickened endosperm cell walls containing mannan polymers. How the radicle is able penetrate these thickened walls to complete seed germination is not clearly understood. The objective of this study was to determine if radicle emergence is related to the production of endo-b-mannanase to weaken the mannan-rich cell walls of the surrounding endosperm region, and/or if the endosperm structure itself is such that it is weaker in the region through which the radicle must penetrate. Methods Activity of endo-b-mannanase in the endosperm and embryo was measured using a gel assay during and following germination, and the structure of the endosperm in juxtaposition to the radicle, and surrounding the cotyledons was determined using fixation, sectioning and light microscopy. Key Results The activity of endo-b-mannanase, the major enzyme responsible for galactomannan cell wall weakening increased in activity only after emergence of the radicle from the seed. Thickened cell walls were present in the lateral endosperm in the hard-seeded species studied, but there was little to no thickening in the micropylar endosperm except in date seeds. In this species, a ring of thin cells was visible in the micropylar endosperm and surrounding an operculum which was pushed open by the expanding radicle to complete germination. Conclusions The micropylar endosperm presents a lower physical constraint to the completion of germination than the lateral endosperm, and hence its structure is predisposed to permit radicle protrusion.
Endo-b-mannanase, lateral endosperm, micropylar endosperm, germination, hard seeded legumes, Phoenix dactylifera.,
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王傲雪, Aoxue Wang?, Xiaofeng Wang, Yanfang Ren, Xuemei Gong and J. Derek Bewley*
Seed Science Research (2005) 15, 219-227,-0001,():
-1年11月30日
Grains of indica rice (Oryza sativa cv. Peiza 67) exhibit an increase in endo-b-mannanase activity, mostly after the completion of germination. According to tissue blots, the initial increase occurs in association with the embryo, and possibly the scutellum, although the largest sustained increase in activity is in the peripheral regions of the endosperm. The aleurone layer, being the only living region of the endosperm, is presumably the site of synthesis and secretion of the enzyme into the non-living, starch-laden region. b-Mannosidase activity is low throughout germination and subsequent seedling growth, particularly in the endosperm regions. Its activity profile does not mimic that of endo-b-mannanase. In the intact grain, gibberellin (GA) causes a relatively small increase in endo-b-mannanase activity, while abscisic acid (ABA) causes a large decrease; this inhibition is overcome to a considerable extent when GA is supplied along with ABA. b-Mannosidase activity is little affected by either GA or ABA. Embryoless half-grains imbibed in water exhibit only a small increase in endo-b-mannanase activity with time of imbibition, showing the necessity for a stimulus from the embryo for this to occur. Incubating half-grains in the presence of GA results in a large increase in enzyme activity; ABA reduces the amount of activity compared to the water controls. GA is capable of reversing the inhibitory effect of ABA with respect to endo-b-mannanase activity. As in the intact grains, b-mannosidase activity in the half-grains is unaffected by either GA or ABA. It is concluded that the major site for the production of endo-b-mannanase activity is the aleurone layer, and this event is influenced by the presence of the embryo; in the absence of the latter, the increase in enzyme activity is stimulated by GA. b-Mannosidase activity is low throughout germination and post-germination, it is not influenced by GA and ABA, and thus its activity is not regulated in a coordinated manner with that of endo-bmannanase.
abscisic acid, aleurone layer, bmannosidase, endo-b-mannanase, gibberellin, Oryza sativa, rice
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王傲雪, 张红霞, 华子春
植物生理学通讯,2002,38(5):471~474,-0001,():
-1年11月30日
文章从铁吸收的生理生化机制出发,对铁吸收中的相关基因进行分析,进而探讨这些基因的潜在利用价值,以此来说明基因工程获得富铁植物的可行性。
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【期刊论文】番茄衰老相关蛋白SENU3在大肠杆菌中的表达及其抗体制备
王傲雪, 赵永娟 王傲雪, 贾琪, 华子春
植物生理学通讯,2005,41(5):208~110,-0001,():
-1年11月30日
研究了SENU3蛋白表达载体的构建、大肠杆菌表达、纯化及其抗体的制备。
番茄衰老相关蛋白SENU3, 原核表达, 割胶., 电洗脱法, 多克隆抗体
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