李玲
1.脱落酸调节花生抗旱性分子机理研究;2.植物组织培养外植体褐变发生的细胞分子生物学研究;3.葛根素形成相关基因克隆和代谢调控研究。
个性化签名
- 姓名:李玲
- 目前身份:
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学术头衔:
博士生导师
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学科领域:
植物学
- 研究兴趣:1.脱落酸调节花生抗旱性分子机理研究;2.植物组织培养外植体褐变发生的细胞分子生物学研究;3.葛根素形成相关基因克隆和代谢调控研究。
李玲,女,博士,教授,博士生导师。广东省第二批“千百十人才工程”省级重点教师,第二届广东省高等学校教学名师。教育部生物教学指导委员会生物技术分会委员(2006-2010),中国植物生理学会生长物质专业委员会主任委员,广东省植物生长调节剂协会理事长,广东省植物学会副理事长。
2006年以来,承担本科生和硕士研究生“植物生长物质”、“植物生长发育的化学控制”、“植物生理学实验”、“细胞生物学进展”等课程的教学任务,主持国家级精品课程“植物生理学”建设。在“细胞生物学”、“植物学”和“微生物与生物化学制药”专业招收研究生。
主持国家自然科学基金面上项目、广东省自然科学基金项目、广东省科学计划项目、广东省教育厅自然科学重点项目等,研究内容有:1.脱落酸调节花生抗旱性分子机理研究;2.植物组织培养外植体褐变发生的细胞分子生物学研究;3.葛根素形成相关基因克隆和代谢调控研究。
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成果数
9
李玲, XIAORONG WAN & LING LI
DNA Sequence, June 2005; 16(3): 217-223,-0001,():
-1年11月30日
A rate-limiting step in abscisic acid (ABA) biosynthesis in plants is catalyzed by 9-cis-epoxycarotenoid dioxygenase (NCED).Here we present the cloning, characterization of a cDNA from dehydrated peanut (Arachis hygogaea L.) leaves that encodes aputative NCED. The 2486-bp full-length cDNA (designated as AhNCED1), obtained by rapid amplification of cDNA ends(RACE), has an open reading frame of 601 amino acid residues and encodes a protein with a calculated molecular weight of66.86 kDa and an isoelectric point of 8.39. Sequence analysis shows that the deduced amino acid sequence of AhNCED1shares high identity with the reported NCED protein sequences. There is a 30-amino-acid chloroplast-targeting peptide at theN-terminus of the AhNCED1 protein predicted by iPSORTalgorithm. Semi-quantification by duplex RT-PCR reveals thatthe expression of AhNCED1 is up-regulated by dehydration and that rehydration represses its expression. The organ specificexpression pattern of AhNCED1 has been examined, which indicates its dominant expression in leaves and stems. Molecularanalysis of the drought-inducible gene of peanut may be useful to investigate the response of agricultural crops to droughtstress.
Rapid amplification of cDNA ends (, RACE), ,, 9-cis-epoxycarotenoid dioxygenase (, NCED), ,, duplex RT-PCR,, dehydration stress,, Arachis hypogaea L.,
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【期刊论文】Genetic transformation of Torenia fournieri L. mediated byAgrobacterium rhizogenes
李玲, Jun Tao, Ling Li *
South African Journal of Botany 72 (2006) 211-216,-0001,():
-1年11月30日
The transformation of Torenia fournieri L. mediated by Agrobacterium rhizogenes was studied. Almost all roots induced by four bacterial strains,R1000, R1601, A4 and R1205 were putative hairy roots. The effects of bacterial strains, bacterial concentration, acetosyringone, silver nitrate and cocultivationpH on Torenia transformation were investigated. Strain R1000, co-cultivation for 3 days, 30Amol L_1 acetosyringone, 4mg L_1 silvernitrate and pH 6.5 in the cultivation medium provided the optimal conditions under which transformation frequency approached 90%.
Torenia fournieri L., , Agrobacterium rhizogenes, Hairy root, Genetic transformation, Acetosyringone, Silver nitrate
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李玲, Xiao-Rong Wan a, b, Ling Li a, *
Biochemical and Biophysical Research Communications 347 (2006) 1030-1038,-0001,():
-1年11月30日
The oxidative cleavage of cis-epoxycarotenoids catalyzed by 9-cis-epoxycarotenoid dioxygenase (NCED) is considered to be the ratelimitingstep in abscisic acid (ABA) biosynthesis. Here we demonstrate that the expression of AhNCED1 gene in peanut plants is significantlyup-regulated by dehydration and high salinity. The AhNCED1 transcript and endogenous ABA both accumulate predominantlyin leaves and stems of peanut in response to dehydration. The considerable up-regulation of AhNCED1 expression by exogenous applicationof ABA suggests a positive feedback control of ABA biosynthesis in peanut. NAA at high concentration increases ABA biosynthesisin peanut plants through up-regulation of the AhNCED1 gene expression. The constitutive expression of the AhNCED1 gene inwild-type Arabidopsis results in an increase of ABA accumulation in transgenic plants in response to drought stress. Ectopic expression ofAhNCED1 gene in 129B08/nced3 mutant Arabidopsis (with impaired AtNCED3 gene involved in ABA biosynthesis under water stress)driven by the AtNCED3 promoter restores its ability to accumulate ABA during drought stress, and reverts its hypersensitivity to nonionicosmotic stress and soil drought. These results indicate that the expression of AhNCED1 gene plays an important role in theregulation of ABA level during water stress, and that water-stress tolerance of Arabidopsis plants can be improved by ectopic expressionof the AhNCED1 gene causing accumulation of endogenous ABA.
Peanut, ABA,, abscisic acid, NCED,, 9-cis-Epoxycarotenoid dioxygenase, Ectopic expression, Transgenic Arabidopsis, Water-stress tolerance
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李玲, 施和平, 潘瑞炽
植物学报,1998,40(5):470~473,-0001,():
-1年11月30日
Hairy root s were obtained in vitro 10 days after inoculation of cucumber (Cu-cumis sativ us L.) cotyledon explant s with the st rains of A grobacteri um rhizogenes R1000and R1601. The f requency of the cotyledon explants transformed by R1000 and R1601 wasup to 87.5% and 88.9%, respectively. All hairy root s induced by the st rains of R1000 and R1601 grew rapidly on solid hormone-free MS medium. The root s incited by A. rhizogenesR1000 could be divided into three phenotypes. The roots of phenotype Ⅰwere similar to thenormal ones,but had more numerous lateral root s. Root s of phenotype Ⅲwere much stouterand shorter, they elongated very slowly and were more highly branched than root s of phenol-type Ⅰ. Root s of phenotype Ⅱwere of intermediate in appearance. However, the root s in-cited by A. rhizogenes R1601 appeared similar to phenotype Ⅰroot s incited by A. rhizo-genes R1000. Transformation was confirmed by opine detection.
发根农杆菌,, 黄瓜,, 毛状根,, 冠瘿碱X
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李玲, 王利国, 郭穗棉
园艺学报,2004,31(6):622~824,-0001,():
-1年11月30日
研究了墨兰接种炭疽菌后SOD、POD和PAL活性的变化。结果显示: 与对照相比, 接种后36h之前PAL 活性变化不大, 36h后活性开始上升, 84h时达到最高;POD在接种后12和60h出现两个活性高峰,且第1峰大于第2峰;SOD在接种后12h达到最高, 之后下降。
墨兰, 炭疽菌, 保护酶活性
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【期刊论文】蝴蝶兰褐变外植体的显微结构观察以及褐变成分的初步分析
李玲, 许传俊, , 李玲*, 李红, 张铭光
园艺学报,2005,32(6):1111~1113,-0001,():
-1年11月30日
对蝴蝶兰褐变外植体进行扫描电镜和显微结构观察, 发现褐变叶片外植体的上表皮已经变形,维管束完全破坏, 被有毒的鞣质填充, 有些薄壁细胞中发现有不明物质沉积。初步分析褐变外植体分泌到培养基中的成分有缩合鞣质。外植体总酚含量和PAL活性随褐变程度加重逐渐增加, 两者呈现显著正相关。
蝴蝶兰, 褐变, 外植体, 显微结构, 鞣质
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【期刊论文】蝴蝶兰外植体褐变发生与总酚含量、PPO、POD和PAL的关系
李玲, 许传俊, , 李玲*
园艺学报,2006,33(3):671~674,-0001,():
-1年11月30日
蝴蝶兰外植体在褐变发生前期PPO和POD活力皆升高, 褐变发生后酶活力下降。同工酶谱分析发现, 培养0d的蝴蝶兰外植体PPO没有酶带出现, 而POD有1条弱带。离体培养2d POD出现3条酶带, 第4天有新酶带Ⅰ发生, 随后消失, 其余3条带, 随培养天数的延长, 酶带活性渐弱。PPO同工酶谱在培养2d出现3条酶带, 迁移率为0128的酶带Ⅲ在培养4 d活性较强, 随后3条酶带减弱。总酚含量和PAL活力随外植体褐变增强而逐渐增加, 两者呈现极显著正相关。
蝴蝶兰, PPO, POD, PAL, 外植体褐变, 总酚
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李玲, 陶均, 谭汝芳 李玲, *
作物学报,2006,32(5):743~748,-0001,():
-1年11月30日
本研究首次报道了发根农杆菌介导的向日葵遗传转化和毛状根形成的优化条件。由发根农杆菌诱导向日葵子 叶和下胚轴形成的根在含卡那霉素(200mg/L,kanamycin)的选择培养基(1/2Ms)上选择,12d后存活的根转到无激素培养 基上能快速生长,经分子检测证实其为毛状根。同时着重研究了不同诱导条件对向日葵毛状根形成的影响。结果发现 适用于毛状根形成的最优条件为菌株R1205,菌液浓度(ID600)1.0,15min感染,共培养3d,培养基pH 6.5,30vmol/L乙酰 丁香酮(acetosyingone)。在此条件下,子叶和下胚轴的毛状根形成率分别达到23.7%和81.6%。
向日葵, 发根农杆菌, 毛状根, 优化条件
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