Embryological investigation was carried out on apomixis in Eulaliopsis binata (Rotz) C. E. Hubb by using paraffin section method. The results indicated that the development pattern of the embryo sac was apospory. In the early stage of the ovule development, a few of the nucellar cells developed into aposporous initial cells, which differentiated later into two forms of mature embryo sac: (1) one form of the mature embryo sac contained one egg cell, one synergid and two polar nuclei; (2) another form possessed one egg cell, two synergids and one polar nucleus. The former occupied about 67.6% and the later only 32.4%. The development pattern of the aposporous embryo sac resembled the Panicum type. Multiple initial cells of apospory might undergo development simultaneously to form two- or multiple-embryo sacs. The ratio of multiple mature embryo sacs in one ovule was 17.7%. No sexual embryo sac was found in the observed ovules. The genesis of the embryo could be classified into two types according to their initial time: (1) the pre-genesis embryo (74%), which originated from unreduced egg cell before the division of the polar nucleus, was observed at one to two days earlier than anthesis. (2) the late-genesis embryo (26%) which was observed at one or two days after anthesis and formation of free endosperm nuclei. The endosperm was derived from the polar nucleus or secondary nucleus without fertilization. The process of the embryonic development followed the sequence of the sexual embryo. The frequency of polyembryony observed was 13%.

To gain insight into the function of plasma membrane intrinsic protein (PIP) genes in apple, two genes, MdPIP1a and MdPIP1b, were isolated. MdPIP1 expression was in accordance with the volume increase during fruit development, which is a loading process of water and solutes. In addition, the expression of MdPIP1 was up-regulated in the stems by osmotic stress. These results indicate that MdPIP1 may play important roles not only in fruit expansion, but also in maintaining water homeostasis under stress conditions.

从40个PstI/MseI酶切的AF凹引物对中筛选出多态性高的7个，对36份枳属特异种质进行了指纹分析。7个引物对共获得539条带，其中146条带具有多态性。各种质材料之司的遗传相似性在0.23～0.98之司，只需结合其中的3个引物对即可区分所有的材料。根据7个AFLP引物对所得谱带的UPGMA聚类分析表明，聚类结果与地理来源没有明显的联系，这可能正反映了近时司内地区之司枳资源的频繁交流。

用SSR标记分析了29份柑橘属及近缘属植物的亲缘关系。7对SSR引物在29个样品中扩增得到114个等位基因，平均每个位点有1613个等位基因。计算匹配系数后用邻接法进行聚类，结果表明，澳洲指橘与柑橘属的亲缘关系很近；SSR位点的高纯合频率支持富民枳种的地位；枳与柑橘属的关系较远，枳不大可能是从柑橘属衍生而来；Swingle的亚属的划分以及田中的原生柑橘类和后生柑橘类的划分界线都不清晰；现代栽培柑橘的起源与大翼橙关系密切；柑橘属的枸橼、柚和宽皮橘都能很好地分离，支持其为现代栽培柑橘的3个基本种的观点。

利用AFLP分子标记技术，结合形态学特征观察和叶片纤维素等成分含量的测定，将来自4个省的9个不同居群的龙须草划分为4 种生态型；窄叶类型（湖北）、浓绿叶类型（广西）、红秆类型（陕西）和宽叶类型（河南）；来自同一地区的不同居群之间；遗传差异很小，聚为同一种生态型，同一居群内不同无融合生殖个体之间未检测到差异，分析了不同生态型之间的遗传差异和亲缘关系，讨论了AFLP技术在无融合生殖植物的分类和遗传学研究中的应用前景。

采用电融合方法诱导澳洲指橘（Microciturs papuana Swingle）悬浮系的生质体与接柑（Citrus reticulata Blanco）叶肉原生质体融合，得到了再生植株。染色体检查表明再生植株为四倍体（2n=4x=36），6个10-mer碱基随机引物PCR扩增结果表明，尽管再生植株带型表现多样，但仍然可以证明为体细胞杂种。对再生植株田间生长异常的现象进行了讨论。

采用RAPD技术，用从200个随机引物筛选的22个10碱基随机引物对148个桃品种进行DXA扩增。根据各品种扩增的RAPD带和聚类结果。分析具特殊位点，并在聚类图中聚在外围、相似系数较低的品种有玉露蟠挑、秋蜜、爱保太、佛尔蒂尼莫蒂尼和金皇后等特殊品种种质。从分子生物学角度。为种质保存和育种提供理论依据。

Leaf derived protoplats of Sour orange (Citrus aurantium L.) were fused electrically with embryogenic protoplasts of Microcitrus papuana Swingle. Plants wee regenerated form the fusion products. Which were characteristic with three types of leaf morphology. Most of the plants were identical fo Sour orange (namely, Leaf-parent-type plant) and two plants had large and thick leaves whereas one plant had bifoliatc and trifoliate leaves. Chromosome examination showed that these plants were diploid with 18 chromosomes (2n=2x=18). RAPD analysis was employed to verify the hybrid characterisics of the plants in the first two types. Four 10-mer arbitrary primers with polymorphism were cho-sen. Band pattern of the plants was similar with the leaf parent (Sour orange) for the primer OPAA-17. Band pattern of the plants was simi-lar with either Sour orange or M. papuana for OPA-08. As for OPA-07 and OPA-04 three kinds of band profiles were deteced. Results of RAPD marker, together with chromosome deter-mination, indicated that all of the analyzed plants were intergeneric diploid somatic hybrids be-tween Sour orange and M. papuana.