The water-soluble antioxidant, chlorogenic acid, was successfully encapsulated in the low cost-high volume yeast cells for the firsttime, as characterized by FT-IR spectra and fluorescence micrograph of the yeast cells, chlorogenic acid and microcapsule.The encapsulation efficiency (EE) of yeast cells could be enhanced significantly (P<0.001) by the treatment of yeast cells with plasmolyserbefore encapsulation. Also, the release characteristics of the obtained yeast-encapsulated chlorogenic acid were evaluated, andits storage stability as a powder were investigated at 25℃/75% relative humidity (RH), 25℃/90% RH and 60℃. It could be clearlydemonstrated that no chemical changes had taken place during the encapsulation, and the yeast-encapsulated chlorogenic acid exhibiteda good stability. This study would be helpful to promote the application of chlorogenic acid. The new yeast-cell-based encapsulationprotocol may have some general interests for maintaining the stability of other water-soluble substances.

长春花生物碱是目前应用最有效的天然植物抗肿瘤药物，目前利用生物工程生产长春花生物碱成果甚微。通过对长春花愈伤组织的诱导条件进行研究，同时对植株各部位的色氨酸脱羧酶（11）c）、过氧化物酶（POD）进行精确测定。认为长春花愈伤组织的诱导眦Ms培养基、激素2，4-Dmg/L和6-BA0.5 mg/L最好，生物碱的合成是植物体多器官共同参与的结果。

With radical global climate change and global warming, high temperature stress has become one of majorfactors exerting a major influence on crop production. In the cotton (Gossypium hirsutum L.)-growing areasof China, especially in the Yangtze River valley, unexpected periodic episodes of extreme heat stressusually occur in July and August, the peak time of cotton flowering and boll loading, resulting in lower bollset and lint yield. Breeding programs for screening high temperature-tolerant cotton germplasm and cultivarsare urgent in order to stabilize yield in the current and future warmer weather conditions. In thepresent study, 14 cotton cultivars were quantified for in vitro pollen germination and pollen tube growth inresponse to temperatures ranging from 10 to 50℃ at 5℃ intervals. Different cotton genotypes varied intheir in vitro pollen germination and pollen tube length responses to the different temperatures. Maximumpollen germination and pollen tube length ranged from 25.2% to 56.2% and from 414 to 682μm, respectively.The average cardinal temperatures (Tmin, Topt, and Tmax) also varied among the 14 cultivars and were 11.8,27.3, and 42.7℃ for pollen germination and 11.8, 27.8, and 44.1℃ for maximum pollen tube length. Variationsin boll retention and boll numbers per plant in field experiments were found for the 14 cotton cultivars andthe boll retention and boll retained per plant on 20 August varied considerably in different years accordingto weather conditions. Boll retention on 20 August was highly correlated with maximum pollen germination(R2=0.84) and pollen tube length (R2=0.64). A screening method based on principle component analysis ofthe combination of pollen characteristics in an in vitro experiment and boll retention testing in the fieldenvironment was used in the present study and, as a result, the 14 cotton cultivars could be classified astolerant, moderately tolerant, moderately susceptible and susceptible to high temperature.

为构建适用于疫苗株筛选的痘苗病毒载体，利用标记瞬时稳定的原理，在痘苗病毒单选择标记载体psc65 的基础上，构建成带有neo 和L acZ 双选择标记的痘苗病毒载体pVI75。为检验载体pVI75的有效性，将HIV-1合成基因syngpnef 插入到载体pVI75 上，构建成转移质粒pVI75-syngpnef，并与天坛株752-1痘苗病毒共转染CEF细胞。筛选得到的重组病毒经PCR 和Dot blot 检验表明，标记基因已被删除，而目的基因被整合到痘苗病毒基因组上。Westem blot 检测结果表明，目的基因的表达正确。痘苗病毒载体pVI75的构建使得疫苗株筛选的工作量大为降低，时间大大缩短，为利用痘苗病毒载体构建重组病毒疫苗株的研究提供了参考。

利用18个家猫微卫星基因座，在东北虎（Panthera tigris sibilia）DNA 中扩增结果有4个基因座没有产物，8个基因座为单态，6个基因座为多态性。同时利用苏门答腊虎的微卫星序列设计了8对引物，在东北虎DNA 中有4对具有多态性。微卫星基因座的多态性百分率为38.5%。在供试的27只东北虎中，发现等位基因间的变异均为偶数碱基长度变化，对有准确谱系记录的个体研究表明，这10个微卫星DNA 遗传标记符合孟德尔遗传规律，所以这些微卫星DNA可以有效的应用于东北虎的亲子鉴定。利用这10对多态性引物，我们成功地鉴定了7个父子关系不清的后代。收集的样品包括23 只毛发样品和4 只血液样品，实验结果表明，毛发和血液样品均可以得到清晰的微卫星条带