邓乐
个性化签名
- 姓名:邓乐
- 目前身份:
- 担任导师情况:
- 学位:
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学术头衔:
博士生导师
- 职称:-
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学科领域:
微生物学
- 研究兴趣:
邓乐,男,1960年出生,理学博士,教授,博士生导师。湖南师范大学生科院微生物系主任,现代微生物技术实验室主任,微生物分子生物学湖南省重点实验室副主任。1978.3—1985.1在湖南师范大学本科和研究生学习。1996年毕业于湖南大学并获理学博士学位,1998 — 2000年先后在美国印第安那大学和加州州立大学洛杉矶分校从事博士后研究工作,2007.7— 2007.12在英国伦敦帝国理工学院访问学习。由于科研和教学工作成绩突出,2002年被湖南省确定为学科带头人培养对象。先后主持国家863计划项目、国家自然科学基金项目等省部级课题二十多项。已在BIOSENS BIOELECTRON, APPL MICROBIOL BLOT, ENZYME MICROB TECH,BIORESOURCE TECHNOL等国内外重要学术刊物上发表论文50余篇。已培养博士硕士10多人。
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1609
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成果阅读
1034
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成果数
20
【期刊论文】Detection of Bifidobacterium Species-specific 16S rDNA Based on QD FRET Bioprobe
邓乐, JunFeng Jiang & ZhiHui Peng & Le Deng & Guang Li & LinLi Chen
J Fluoresc (2010) 20: 365-369,-0001,():
-1年11月30日
Fluorescence resonance energy transfer (FRET) that consists of quantum dot as donors and organic fluorophore dyes as acceptors has been a very important method to detect biomolecules such as nucleic acids. In this work, we established a new FRET detection system of Bifidobacterium species-specific 16S rDNA using QD—ROX FRET bioprobe, in which 525 nm QD-DNA conjugation consisted of the carboxyl-modified QD and the amino-modified DNA in the presence of EDC. Both ROX-DNA and the conjugation above could hybridize with the target DNA after forming the QD—ROX bioprobe. When the hybridization made the distance between the QD and ROX to meet FRET effect needed, 525 nm QD fluorescence intensity decreased and ROX fluorescence intensity increased. In the control, there was no notable change of fluorescence intensities without target DNA. It is very clear that the change of the QD and ROX fluorescence intensities provide the good base and guaranty for this rapid and simple detection system.
Quantum dots · Sequence alignment · Bioprobe · FRET · 16S rDNA detection
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邓乐, Huidong Li a, b, Ting Liu a, Zhao Li a, Le Deng a, *
Bioresource Technology 99 (2008) 2234-2241,-0001,():
-1年11月30日
The main goal of this study was to exploit low-cost and efficient sorbents for the removal and recovery of Cr (VI) in wastewater. Three supports of sawdust, polyurethane and alginate were applied to immobilize living and dead R. cohnii cells, respectively. There was a distinct increase in the Cr (VI) removal efficiency before and after the HCl-pretreatment. Langmuir adsorption isotherm model was well used to describe the distribution of Cr (VI) between the liquid and solid phases in batch studies. The values of q0 predicted by Thomas model were near to experimental ones in the experiments of packed column. The breakthrough curves calculated with this model were consistent well with experimental ones at a largely extent. Desorption, regeneration and reuse of the packed column were studied. After 5 cycles, adsorption capacity was still kept at higher level, reaching to 91.4, 87.9, 91.4 and 93.3 mg/l contrasted with the first cycle (94.1, 90.4, 94.8 and 98.5 mg/l) and the desorption efficiency were 85.0%, 96.2%, 93.4% and 91.4% compared with the first cycle (87.6%, 95.4%, 96.7% and 94.3%), corresponding to living cells immobilized with sawdust, polyurethane, and dead cells immobilized with polyurethane and alginate, respectively. The results indicated that the packed columns with the immobilized living and dead R. cohnii cells were the better option to adsorb, desorb and recover Cr (VI) from wastewater.
Hexavalent chromium, Rhizopus cohnii, Immobilization, Packed column, Biosorption
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邓乐, Huidong Li a, b, Zhao Li a, Ting Liu a, Xiao Xiao a, Zhihui Peng a, Le Deng a, *
Bioresource Technology 99 (2008) 6271-6279,-0001,():
-1年11月30日
The goal of this study was to develop an applied technique for the removal and recovery of heavy metal in wastewater. It is novel that the Cr (VI) could be adsorbed and recovered by bio-functional magnetic beads. Furthermore, the magnetic separation technology would make their separation more convenient. The beads were constituted by the powder of Rhizopus cohnii and Fe3O4 particles coated with alginate and polyvinyl alcohol (PVA). The parameters effecting Cr (VI) removal were obtained: the optimum pH 1.0 and optimum temperature 28 C. The biosorption took place mainly in form of Cr (VI) and R. cohnii biomass played a key role in Cr (VI) adsorption. The model of Langmuir isotherm and Lagergren could be better used to fit the sorption process and kinetics, respectively. The beads still maintained predominant characteristics of adsorption, recovery and magnetism after five cycles for adsorption-desorption. The mechanism of adsorption was gained by Fourier transform infrared spectroscopy (FTIR), raman spectroscopy (RS) and scanning electron microscopy (SEM). The groups of-NHþ3,-NHþ2-, and NH- played an important role in the Cr (VI) adsorption. Consequently, the beads exhibited the superior performances in Cr (VI) cleanup, separation and recovery and the perspective potential in application. 2008 Elsevier Ltd. All rights reserved.
Biosorption, Cr (, VI), , Rhizopus cohnii, Removal, Bio-functional magnetic beads
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【期刊论文】A new method for the detection of ATP using a quantum-dot-tagged aptamer
邓乐, Zhang Chen & Guang Li & Lan Zhang & Junfeng Jiang & Zhao Li & Zhihui Peng & Le Deng
Anal Bioanal Chem (2008) 392: 1185-1188,-0001,():
-1年11月30日
Fluorescence resonance energy transfer (FRET) between a quantum dot as donor and an organic fluorophore as acceptor has been widely used for detection of nucleic acids and proteins. In this paper, we developed a new method, characterized by 605-nm quantum dot (605QD) fluorescence intensity increase and corresponding Cy5 fluorescence intensity decrease, to detect adenosine triphosphate (ATP). The new method involved the use of three different oligonucleotides: 3′-biotin-modified DNA that binds to streptavidin-conjugated 605QD; 3′-Cy5- labelled DNA; and a capture DNA consisting of an ATP aptamer and a sequence which could hybridize with both 3′-biotin-modified DNA and 3′-Cy5-labelled DNA. In the absence of the target ATP, the capture DNA binds to 3′- biotin-modified DNA and 3′-Cy5-labelled DNA, bringing quantum dot and Cy5 into close proximity for greater FRET efficiency. When ATP is introduced, the release of the 3′- Cy5-labelled DNA from the hybridization complex took place, triggering 605QD fluorescence intensity increase and corresponding Cy5 fluorescence intensity decrease. Taken together, the virtue of FRET pair 605QD/Cy5 and the property of aptamer-specific conformation change caused by aptamer-ATP interaction, combined with the fluorescence intensity change of both 605QD and Cy5, provide prerequisites for simple and convenient ATP detection.
Aptamer·Quantum dot·FRET·ATP detection
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邓乐, 李兆, 李会东, 刘婷, 邓乐*
生命科学研究:2008,12(3):232~236,-0001,():
-1年11月30日
研究固定化黄孢原毛平革菌对水溶液中2,4-二氯酚(2,4-DCP)的降解效果,探讨固定化黄孢原毛平革菌处理水溶液中氯酚类污染物的可行性。结果表明,采用固定化方法处理的白腐真菌,其产酶稳定性及酶活均比游离态白腐真菌有显著提高。2,4-DCP降解效果受固定化孢子接种量、pH值、摇床转速、2,4-DCP的初始浓度和表面活性剂浓度的影响。当pH为4.5,摇床转速180r/min,培养基含有1%的Tween80,2,4-DCP初始浓度为40mg/L时,加入10mL固定化白腐真菌孢子,2,4-DCP去除效果最好。
固定化白腐真菌, 生物降解, 2,, 4-二氯酚
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邓乐, Lingli Chena, b, Le Denga, ∗, Linlin Liu a, Zhihui Peng a
Biosensors and Bioelectronics 22 (2007) 1487-1492,-0001,():
-1年11月30日
The aim of this study was to establish an IMS-MS/SPR technique for the detection of Staphylococcus aureus (S. aureus) and Staphylococcus protein A (SPA) at the same time, which consists of isolating S. aureus and trapping-enrichmenting its SPA by IMS, and the end point is determined by using either MS or SPR measurements. Magnetic bead (MB) containing aldehyde group was synthesized with latex-polymerization and immunomagnetic bead (IMB) was fabricated by modifying its surface with an oriented layer of human IgG in covalent linkage. As soon as sample of pulverator-treated bacterial cell lysate (108 cfu/mL) was incubated with IMB at 4 ◦C for 30 min, SPA was captured and separated from the mixed solution in a few minutes by the IMB and then detected with mass spectrometry after washing. SPR was used to detect S. aureus quantitatively in situ at the end-detection procedure. All in all, this technique can be employed to detect rapidly SPA and S. aureus within 2 h and also be applied to detect other cells or their membrane proteins with changed modified antibodies.
S., aureus, SPA, IMS-MS/, SPR
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邓乐, Wei Wang a, b, Le Denga, ∗, Zhi Hui Peng a, Xiao Xiao a
Enzyme and Microbial Technology 40 (2007) 255-261,-0001,():
-1年11月30日
Magnetic hydroxyl microspheres were synthesized and activated by using epoxyl chloropropane by means of suspension polymerization. The effects of amount of crosslink agent on the immobilization yield and activity yield of immobilized enzyme were investigated. The results showed that the magnetic polymer with crosslink density 30% could be used as an optimized immobilized penicillin G acylase (PGA) support. The apparent Michaelis constant Km and Vmax of the immobilized enzyme were 8.312×10−5 mol/l and 21.64 mol/min, respectively. The protecting immobilization was achieved by employing the interaction between the phenyl acetic acid (PAA) and PGA. It was found that activity yield of immobilized PGA was 1.0834-fold of the randomly immobilized enzyme. The optimal pH and temperature of the immobilized enzyme were 8.5 and 45◦C, respectively, for hydrolysis of penicillin G. Being applied for 80 times, the remained activity of the immobilized enzyme was about 94.2%.
Magnetic hydroxyl microspheres, Immobilized penicillin G acylase, Phenyl acetic acid
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【期刊论文】用序列比对设计的茎环结构探针检测金黄色葡萄球菌*
邓乐, 杨旭, 肖潇, 陈章, 李会东, 邓乐**
微生物学通报:2007,34(6):1169~1173,-0001,():
-1年11月30日
基于金黄色葡萄球菌16SrRNA基因序列,采用序列比对设计了一种茎环结构的寡聚核苷酸探针。探针的环序列即为金黄色葡萄球菌16SrRNA基因序列的其中一个片段,同其他菌种的16SrRNA基因序列误配2个以上的核苷酸,因此能高度专一、灵敏的检测金黄色葡萄球菌16SrRNA。根据分子信标技术和酶联免疫分析的原理,评估一个实验方法,即利用能构象转换的、固定化的茎环结构探针酶联检测靶核酸。由于探针的特异性加强,这个检测系统能有效的排除假阳性即不会出现误配一个核苷酸的情况。采用微量浓度测定分析,最低下限可检测出大约4ng的金葡球菌16SrRNA。这种方法的灵敏度比其他常规检测方法高出了至少一个数量级。
序列比对,, 茎环结构探针,, 16S核糖体核糖核酸,, 检测
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邓乐, 叶从勇, 杨旭
生命科学研究:2006,10(3):55~65,-0001,():
-1年11月30日
分子信标是一种高灵敏度、高特异性的新型荧光核酸探针。它在与互补DNA或RNA靶序列杂交时放出荧光。利用Gemebank中调出已知HBV病毒ayr亚型基因组信息,通过Beacon Designer4.0软件进行分子信标探针设计,共设计出6条分子信标探针,以便于为目前HBV病毒快速诊断提供参考。
乙肝病毒, 分子信标探针, DNA序列, 引物
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邓乐, 刘琳琳, 曾力希, 刘婷, 邓乐*
生物工程学报:2005,21(5):789~793,-0001,():
-1年11月30日
以磁性金属螯合琼脂糖微球为载体,利用金属螯合配体(IDACu2+)与蛋白质表面供电子氨基酸相互作用的原理,定 向固定了木瓜蛋白酶。固定化最适条件为Cu2+1.5×10-2mo/g载体、固定化时间4h、固定化pH7.0、给酶量30mg/g载体。固定化酶的最适反应温度70、最适反应pH8.0,固定化酶的热稳定性明显高于溶液酶,固定化酶活力回收为68.4%,且有较好的操作稳定性,载体重复使用5次后固定化酶酶活为首次固定化酶79.71%。
金属整合载体,, 定向固定化,, 固定化酶,, 木瓜蛋白酶
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