郭旺珍
遗传学,基因组学和分子育种,生物信息学。
个性化签名
- 姓名:郭旺珍
- 目前身份:
- 担任导师情况:
- 学位:
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学术头衔:
博士生导师, 教育部“新世纪优秀人才支持计划”入选者
- 职称:-
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学科领域:
作物育种学与良种繁育学
- 研究兴趣:遗传学,基因组学和分子育种,生物信息学。
郭旺珍,女,博士,教授
简历:
1987.9-1991.7 山西农业大学农学专业,学士
1991.9-1994.7 山西农业大学作物遗传育种专业,硕士
1994.9-1997.7 南京农业大学作物遗传育种专业,博士
1997.8-1999.12 南京农业大学农学院,讲师
2000.1-2004.3 南京农业大学农学院,副教授,硕士生导师
2004.4-今 南京农业大学农学院,教授,博士生导师
从事的研究领域:遗传学,基因组学和分子育种,生物信息学。
研究课题来源:国家863,973,国家自然科学基金,科技部转基因专项等国家级项目及教育部,江苏省等省部级项目近20项。
主要学术成就:构建了国际上第一张含功能标记最多的四倍体栽培棉种遗传图谱。基于该图谱,系统进行了不同棉种标记可转移性及比较基因组学研究和D基因组棉种的分化研究。标记定位了大量棉花育种目标性状基因/QTLs并用于分子标记辅助选择研究。克隆和验证了一批高品质棉纤维优势表达的基因或全长cDNA,为优质棉种质创新提供了重要的基因资源。建立了MAS的基因叠加育种体系,显著提高了育种效率,获得南农85188等一批抗虫、优质的创新种质。利用 MAS育种体系培育出高产、优质、抗虫的棉花杂交种2个,并进行产业化开发。
在Genetics、BMC Genomics、TAG、MGG、Mol Breed、Plant Sci等国内外专业杂志发表论文130多篇,其中SCI论文50余篇。参编论著3部,获国家、省部级成果奖6项,获授权专利5项。
获中国青年科技奖、江苏省五一巾帼标兵、江苏省三八红旗手,教育部新世纪优秀人才、中国农学会青年科技奖、南京市有突出贡献中青年专家、南京农业大学优秀教师等荣誉称号多项。
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成果数
4
郭旺珍, Dayong Zhang, Tianzhen Zhang, Wangzhen Guo *
Journal ofPlantPhysiology167(2010)393-399,-0001,():
-1年11月30日
Single celled fibers initiate at anthesis from cotton seed epidermal cells of normal developmental cotton cultivars; however, fiber initiation is retarded in some cotton fiber mutants. In this study, the relationship between genes associated with fiber initiation retardation and fiber initiation development was investigated using three cotton fiber developmental mutants: recessive naked seed n2; dominant naked seed N1; and Xinxiangxiaoji Linted Fuzzless Mutant (XinFLM); with genetic standard line TM-1 (TM-1) as control. Retardation during fiber initiation development was observed in N1 and XinFLM by scanning electron microscope (SEM) analysis. Reverse transcription polymerase chain reaction (RT PCR) analysis of genes related to the fiber initiation development showed that the expression of GhEXP1 and GhMYB25 was lower in N1 and XinFLM than in TM-1 and n2, however, the expression of GhTFG1 and GhTFG3 in XinFLM and n2 was higher than in TM-1 and N1. In rive and in vitro treatments on ovules demonstrated that 30% hydrogen peroxide (H202) could prevent fiber initiation retardation in XinFLM, but no evident effect on N1. To further confirm the relationship between gene expression and the effects of H2O2 in XinFLM, qRT PCR analysis of four differentially expressed genes was performed using-1 d post anthesis (DPA) ovules of XinFLM treated for 24 and 48h with 30% H2O2 and H2O, respectively, with 0 and 1 DPA untreated ovules fiom XinFLM and TM-1 as control. The results showed that the expression of GhMYB25 and GhEXP1 showed significant difference in XinFLM after-1 DPA ovule treated for 24h relative to the untreated or H2O treated ovules, with the expression of GhMYB25 increased significantly and that of GhEXP1 decreased. This implied that H2O2 might be one of the upstream signal molecules affecting the expression of GhMYB25 and GhEXP1 genes. The fiber initiation retardation in XinFLM might be related to the production of reactive oxygen species (ROS).
Cotton flberinitiation H2O2 Retardation development RT-PCR SEM
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【期刊论文】A preliminary analysis of genome structure and composition in Gossypium hirsutum
郭旺珍, Wangzhen Guo†, Caiping Cai†, Changbiao Wang, Liang Zhao, Lei Wang and Tianzhen Zhang*
Page 2 of 18 (page number not for citation purposes),-0001,():
-1年11月30日
Background: Upland cotton has the highest yield, and accounts for > 95% of world cotton production. Decoding upland cotton genomes will undoubtedly provide the ultimate reference and resource for structural, functional, and evolutionary studies of the species. Here, we employed GeneTrek and BAC tagging information approaches to predict the general composition and structure of the allotetraploid cotton genome. Results: 142 BAC sequences from Gossypium hirsutum cv. Maxxa were downloaded http:// www.ncbi.nlm.nih.gov and confirmed. These BAC sequence analysis revealed that the tetraploid cotton genome contains over 70,000 candidate genes with duplicated gene copies in homoeologous A- and D-subgenome regions. Gene distribution is uneven, with gene-rich and gene-free regions of the genome. Twenty-one percent of the 142 BACs lacked genes. BAC gene density ranged from 0 to 33.2 per 100 kb, whereas most gene islands contained only one gene with an average of 1.5 genes per island. Retro-elements were found to be a major component, first an enriched LTR/gypsy and second LTR/copia. Most LTR retrotransposons were truncated and in nested structures. In addition, 166 polymorphic loci amplified with SSRs developed from 70 BAC clones were tagged on our backbone genetic map. Seventy-five percent (125/166) of the polymorphic loci were tagged on the D-subgenome. By comprehensively analyzing the molecular size of amplified products among tetraploid G. hirsutum cv. Maxxa, acc. TM-1, and G. barbadense cv. Hai7124, and diploid G. herbaceum var. africanum and G. raimondii, 37 BACs, 12 from the A- and 25 from the D-subgenome, were further anchored to their corresponding subgenome chromosomes. After a large amount of genes sequence comparison from different subgenome BACs, the result showed that introns might have no contribution to different subgenome size in Gossypium. Conclusion: This study provides us with the first glimpse of cotton genome complexity and serves as a foundation for tetraploid cotton whole genomesequencing in the future.
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郭旺珍, Wangzhen Guo*, Caiping Cai*, Changbiao Wang, Zhiguo Han, Xianliang Song, Kai Wang, Xiaowei Niu, Cheng Wang, Keyu Lu, Ben Shi, Tianzhen Zhang**
,-0001,():
-1年11月30日
The mapping of functional genes plays an important role in studies of genome structure, function, and evolution, as well as allowing gene cloning and marker-assisted selection to improve agriculturally-important traits. Simple sequence repeats (SSRs) developed from expressed sequence tags (ESTs), EST-SSR (eSSR), can be employed as putative functional marker loci to easily tag corresponding functional genes. In this paper, 2,218 eSSRs, 1,554 from G. raimondii-derived and 754 from G. hirsutum-derived ESTs, were developed and used to screen polymorphisms in order to enhance our backbone genetic map in allotetraploid cotton. Out of 1,554 G. raimondii-derived eSSRs, 744 eSSRs were able to successfully amplify polymorphisms between our two mapping parents, TM-1 and Hai7124, presenting a polymorphic rate of 47.9%. However, an only 23.9% (159/754) polymorphic rate was produced from G. hirsutum-derived eSSRs. No relationship was observed between the level of polymorphism, motif type, and tissue origin, but the polymorphism appeared to be correlated with repeat type. After integrating these new eSSRs, our enhanced genetic map consists of 1,790 loci in 26 linkage groups and covers 3425.8 cM with an average inter-marker distance of 1.91 cM. This microsatellite-based, gene-rich linkage map contains 71.96% functional marker loci, of which 87.11% are eSSR loci. There were 132 duplicated loci bridging 13 homeologous At/Dt chromosome pairs. Two reciprocal translocations after polyploidization between A2 and A3, and between A4 and A5 chromosomes were further confirmed. A functional analysis of 975 ESTs producing 1,122 eSSR loci tagged in the map revealed that 60% had clear BLASTX hits (<1e-10) to the Uniprot database and that 475 were mainly associated with genes belonging to the three major gene ontology categories of biological-process, cellular-component, and molecular-function; many of the ESTs were associated with two or more category functions. The results presented here will provide new insights for future investigations of functional and evolutionary genomics, especially those associated with cotton fiber improvement.
cotton,, functional marker,, genetic mapping
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郭旺珍, W.Z. Guo, Z.Q. Sang, B.L. Zhou, T.Z. Zhang *
Plant Science 172(2007)808-814,-0001,():
-1年11月30日
The tetraploid cotton species, which includes two commercially important species, Gossypium hirsutum L. and Gossypium barbadense L., were synthesized by A and D compound genomes. There are two A-genome species and 13 D-genome species in Gossypium. The A-genome species are distributed throughout Africa and Asia, and the D-genome species occur primarily in Mexico, but also in Peru, the Galapagos Islands and Arizona in the United States. There is a clear genetic relationship between the two A-genome species; however, genetic relationships among the D-genome species remain unclear. We randomly chose 324 expressed sequence tag-simple sequence repeat (EST-SSR) primer pairs to analyze the genetic relationships of the D-genome diploid cotton (Gossypium L.), using the A-genome species as the out-group. The primer pairs were developed from 7 to 10 day post-anthesis (dpa) fiber cDNA library of diploid A-genome G. arboreum, and from 3 to 3 dpa cDNA library and the first-true-leaves library in G. raimondii, for the A-genome and D-genome species, respectively. Both independent and combined analyses of the two types of ESTSSRs resolved that A- and D-genome species could be easily grouped. Further, each type of EST-SSR was effective in distinguishing the respective in-group species. Following the combined analyses, 12 D-genome species were clustered into six groups in complete agreement with current subsection taxonomy. However, there was an intersecting relationship among D-genome species at the section level, indicating that cotton species belonging to the different sections likely had close genetic relationships and, therefore, no distinctive boundaries exist between the two sections. Based on the larger sampling of combined EST-SSR markers, molecular data supplied new proof that there was modest genetic affinity between G. raimondii belonging to subsection Austroamericana and G. gossypioides belonging to subsection Selera (Ulbrich) Fryxell at the genomic level. The relationships between G. raimondii, G. davidsonii and G. klotzschianum belonging to different sections are also discussed in the paper.
Gossypium, Houzingenia, Cotton, EST-SSR, Genetic relationship
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