李国清
长期从事昆虫生物化学和化学生态学研究。
个性化签名
- 姓名:李国清
- 目前身份:
- 担任导师情况:
- 学位:
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学术头衔:
博士生导师
- 职称:-
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学科领域:
农业昆虫学
- 研究兴趣:长期从事昆虫生物化学和化学生态学研究。
李国清,教授,博导,主持国家自然科学基金项目2项、江苏省“九五”重点基金1项、国际合作项目1项。
长期从事昆虫生物化学和化学生态学研究。产卵忌避信息素及昆虫-植物化学通讯的研究结果得到国际同行的赞誉。
发表论文40篇,其中SCI论文5篇。获省部级科技进步将5项。
主持的主要科研项目:
* 源于棉铃虫幼虫粪便的诱导子的鉴定及对植物防御的诱导, 2007-01,2009-12.
* 脂肪酸诱导子的鉴定及激发植物防御的信号转导途径, 无.
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5
李国清, Mulan Liu, Hejuan Yu, Guoqing Li
Journal of Insect Physiology 54 (2008) 656-662,-0001,():
-1年11月30日
Our previous results showed that an extract of the abdomens of mated females of the cotton bollworm, Helicoverpa armigera, deterred oviposition of conspecifics. In this paper, we found that the extract, in addition to reducing conspecifics' oviposition, could elicit strong electroantennogram responses from mated females. The EAG elicitors mainly came from mature chorionated eggs in the ovarioles. Laid eggs or their surface extract evoked similar EAG responses. The main chemical components of the EAG elicitors from mature chorionated and laid eggs were myristic, palmitic, stearic, and oleic acids. A blend of authentic fatty acids at the ratio found in the laid eggs and in an amount equivalent to 100 laid eggs mimicked the EAG response and deterring effect. Moreover, these four ovipositiondeterring fatty acids and their corresponding methyl esters evoked significantly higher EAG responses from both mated females and males than hexane blank and background. The EAG values differed among the test chemicals and between sexes. These results demonstrate that the four fatty acids from eggs are at least partially responsible for the oviposition-deterring effect of the extract from the abdomens of mated female H. armigera and that the moths may detect these chemicals olfactorily by antennae.
Helicoverpa armigera, Eggs, Oviposition deterrents, Fatty acids, EAG
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李国清, GUOQING LI, , * and YUKIO ISHIKAWA
Journal of Chemical Ecology, Vol. 32, No.3, March 2006,-0001,():
-1年11月30日
Extraction, fractionation, and gas chromatographyjmass spectrometry analyses guided by bioassays have shown that n-alkanes and free fatty acids in leaf epicuticular wax of the Japanese knotweed Fallopia (Reynoutria) japonica stimulate oviposition in the Far-Eastern knotweed borer, Ostrinia latipennis (Lepidoptera: Crambidae). n-Alkanes made up 48.1% of the total amount of epicuticular wax, and their carbon chain length was in the C16jC33 range, with n-nonacosane (n-C29) most abundant, followed by n-C27, n-C25, and n-C31. Free fatty acids with C9jC22 accounted for 22.3%, and hexadecanoic acid was predominant. A mixture of authentic n-alkanes and fatty acids of the composition found in the epicuticular wax, a mixture of n-alkanes, and a mixture of fatty acids significantly enhanced oviposition. Thus, it was demonstrated that both n-alkanes and free fatty acids in leaf epicuticular wax of F. japonica are naturally occurring oviposition stimulantsfor O. latipennis.
Fallopia japonica,, Ostrinia latipennis,, alkane,, fatty acid,, oviposition stimulant.,
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李国清, Haiyan Xu, Guoqing Li, Mulan Liu, Guangnan Xing
Journal of Insect Physiology 52 (2006) 320-326,-0001,():
-1年11月30日
Oviposition deterrents in the frass of cotton bollworm (CBW), Helicoverpa armigera larvae fed on an artificial diet (FA) and on cotton Gossypium hirsutum leaves (FC) were investigated by behavioral bioassays and electroantennography analyses in the laboratory. It was found that a water suspension or a hexane extract of the frass FA or FC, in contrast to the corresponding foods, significantly deterred oviposition of conspecifics. When hexane extracts of the frass FA and FC were further partitioned into polar and neutral lipid fractions, two polar fractions significantly reduced oviposition. The neutral fraction from frass FC also exhibited significant deterrence, although the activity was much lower than that of the corresponding polar fraction. The polar lipid fractions contained several fatty acids, mainly palmitic and oleic acid at the ratio nearly 1:1. A blend of authentic fatty acids of the same composition found in frass FA or FC mimicked the deterring effect. Moreover, these fatty acids and their blend at the ratio found in frass FA or FC elicited significant electroantennogram responses and typical dose–response curves. Thus, it is suggested that CBW larvae may deploy two types of oviposition deterrents: a non-specific and a specific one. The former is a blend of fatty acids, independent of food and plays an important role in oviposition deterrence, whereas the latter may be produced only when the larvae feed on cotton leaves. The possible explanations of this deployment have also been discussed.
Helicoverpa armigera, Larval frass, Oviposition deterrents, Fatty acids
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【期刊论文】OVIPOSITION DETERRENTS IN LARVAL FRASS OF FOUR Ostrinia SPECIES FED ON AN ARTIFICIAL DIET
李国清, GUOQING LI, and YUKIO ISHIKAWA, ∗
Journal of Chemical Ecology, Vol. 30, No.7, July 2004,-0001,():
-1年11月30日
Behavioral bioassays have shown that volatile oviposition-deterring chemicals are present in the frass of Ostrinia zealis, O. furnacalis, O. scapulalis, and O. latipennis larvae fed on an artificial diet. These chemicals were extractable with acetone, and could be partitioned into a polar lipid fraction. This fraction mainly included palmitic, stearic, oleic, linoleic, and linolenic acids. No significant differences among the four Ostrinia species were found in the amount and composition of these free fatty acids. A mixture of the five authentic fatty acids of the composition found in the larval frass of O. zealis exhibited significant oviposition-deterring effects on all four species.
Ostrinia,, larval frass,, oviposition deterrents,, fatty acids.,
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李国清, Li Guoqing *, Han Zhaojun, Mu Lili, Qin Xiaoran, Chen Changkun, Wang Yinchang
Journal of Insect Physiology 47 (2001) 951-956,-0001,():
-1年11月30日
Observation of the oviposition behavior of cotton bollworm demonstrated the existence of substances that can act as oviposition deterrents. The scales, abdomens and tarsi of the moths, the most likely carrier, were extracted for the substances. An acetone extract exhibiting an oviposition deterrent effect was analyzed using gas chromatography and combined gas chromatography-mass spectroscopy. Three components, 4-methyl-4-hydroxyl-pentanone-2, hexadecanoic acid and (Z)-9-octadecenoic acid, were identified, being present in the ratio 1: 2: 2. Experiments with synthetic mixtures indicated that a mixture of equal amounts of the two organic acids exhibited similar repellent and deterrent activity to a synthetic mixture of all three components. It is concluded that the natural oviposition-deterring pheromone is a mixture of hexadecanoic acid and (Z)-9-octadecenoic acid.
Helicoverpa armigera, Tarsus, Oviposition-deterring pheromone
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