刘凤权
植物病细菌,生物防治,植物检疫与外来物种管理,化学污染物快速检测。
个性化签名
- 姓名:刘凤权
- 目前身份:
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学术头衔:
博士生导师
- 职称:-
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学科领域:
植物病理学
- 研究兴趣:植物病细菌,生物防治,植物检疫与外来物种管理,化学污染物快速检测。
刘凤权,博士,男,1964年生,南京农业大学植保学院教授、博士生导师,中国生物化学学会理事,江苏省植病学会理事,江苏省“青蓝工程”优秀青年骨干教师。长期从事植物病理学和植物病害生物防治、植物检疫和外来物种管理、转基因植物、农产品质量安全检测等方面的教学和研究工作。“十五”以来,先后赴美国Tulane大学和荷兰瓦赫宁根大学进行学术交流与合作研究;主持国家自然科学资金、国家973计划、863计划和国家攻关(支撑)计划、农业行业科技专项、转基因植物专项等课题15项;先后在《Analytica Chimica Acta》、《Food Chemistry》、《Journal of Agricultural and Food Chemistry》、《Biological Control》、《中国农业科学》、《应用生态学报》等国内外著名学术刊物上发表学术论文40余篇;获得省部级科技成果奖3项;获得和申请国家发明专利10多项。应邀主编《园艺植物病理学》(1999,中国农业出版社)以及面向21世纪教材《园艺植物病理学》(2000,中国农业大学出版社)各一部。
获得荣誉:
1、2004年度江苏省青蓝工程优秀青年骨干教师;
2、2007年度教育部科技进步一等奖(排名第一),国家级精品课程(普通植物病理学);
3、2007年度教育部科技进步一等奖(排名第三),重要植物病原生物快速分子检测技术研究及其应用。
研究方向: 植物病细菌,生物防治,植物检疫与外来物种管理,化学污染物快速检测。
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【期刊论文】Determination of N-Methylcarbamate Insecticide Metolcarb by Enzyme-Linked Immunosorbent Assay
刘凤权, Zhang Qi, Li Tiejun, Zhu Xiaoxia, Xu Lina, Liu Fengquan, *, Hu Baishi, Jiang Yinghua, Cao Bin
Chin J Anal Chem, 2006, 34 (2), 178−182.,-0001,():
-1年11月30日
The hapten of the N-methylcarbamate insecticide metolcarb, 3-{[1-(3-(methyl)phenyloxy)carbonyl]amino}propanoic acid (HOM), was synthesized and conjugated with the carrier proteins bovine serum albumin (BSA) and ovalbumin (OVA), respectively, by the active ester method. The conjugate of HOM-BSA was used as immunogen, and we prepared rabbits' anti-metolcarb serum, the titres of which were determined by noncompetitive indirect ELISA procedures and reached 1.28×106. The results of the cross reaction indicate that the antiserum produced could specially recognize the insecticide metolcarb. After optimization of the ELISA conditions, such as ionic strengths, organic solvents, pH values, blocking agents, and so on, the proper parameters of ELISA procedure were determined, and an assay protolcol for the N-methylcarbamate insecticide metolcarb was established. On the basis of statistical analysis, the linear range of determination was from 1 to 104 ng/mL, IC50 was 40.74 ng/mL, limit detection was 0.08–0.10 ng/mL, intra-assay coefficient of variation (CV) reached 2.9%, and inter-assay CV reached 4.6%. The recoveries obtained by standard metolcarb addition to different samples such as rice, water, and soil were 80, 93.4, and 107%, respectively. This is the first time that polyclonal antibodies to the N-methylcarbamate insecticide metolcarb are produced, and the ELISA, used here for efficient and available detection of metolcarb, may become a convenient and satisfied analytical tool for monitoring metolcarb residues in environmental and agricultural samples.
N-Methylcarbamate insecticide, Metolcarb, Hapten, Antigen, Antibody, Enzyme-linked immunosorbent assay
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