陈同辛
目前研究方向为免疫功能低下、反复感染、原发性免疫缺陷病和风湿性疾病。在原发性免疫缺陷病方面的研究处于国内领先水平,目前正在从事病毒感染对新生儿树突状细胞分化和功能影响的研究。
个性化签名
- 姓名:陈同辛
- 目前身份:
- 担任导师情况:
- 学位:
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学术头衔:
博士生导师
- 职称:-
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学科领域:
儿科学
- 研究兴趣:目前研究方向为免疫功能低下、反复感染、原发性免疫缺陷病和风湿性疾病。在原发性免疫缺陷病方面的研究处于国内领先水平,目前正在从事病毒感染对新生儿树突状细胞分化和功能影响的研究。
陈同辛,男,主任医师,教授,博士生导师,主要从事儿科临床免疫学的临床和研究工作,目前研究方向为免疫功能低下、反复感染、原发性免疫缺陷病和风湿性疾病。在原发性免疫缺陷病方面的研究处于国内领先水平,目前正在从事病毒感染对新生儿树突状细胞分化和功能影响的研究。已完成和正在承担国家级课题及上海市课题10余项,获上海市科技成果进步奖三等奖1项,获上海市医学科技成果奖三等奖2项,在国内外期刊发表论文60余篇,参编著作10部。目前担任中华医学会儿科专科委员会免疫学组副组长,中国免疫学会临床免疫分会副主任委员,上海市免疫学会常务理事兼秘书长,国家自然科学基金委员会评审专家,中华医学会医疗事故技术鉴定专家,国务院政府特殊津贴专家,中华儿科杂志编委,American Academy of Allergy, Asthma and Immunology国际会员,British Journal of Hematology特邀审稿专家。
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陈同辛, LI Li, JIN Ying-ying, CAO Rui-ming and CHEN Tong-xin
Chin Med J 2010 123(10): 1278-1282,-0001,():
-1年11月30日
Background Leukocyte adhesion deficiency type 1(LAD-1)is a rare, autosomal recessive inherited immunodeficiency dlsease characterized by recu rrent severe bacterial lnfection lmpal red pus formation poor wound healing associated with the mutation ln the CD18 gene responsible for the abilitv of the leucocytes to mig rate from the blood stream towards the site of lnflammation Correct and early dlagnosis of LAD-1 is vital to the success of treatment and prevention of agg ressive infections The pu rpose of this study was to collect the clinical findings of the disease and to identify the genetic entity. Methods CDl 8 expression ln the perlpheral blood leukocytes from the patient hls parents and normal control was measu red with flow cytometry The enti re coding regions of the CDl 8 gene were screened with direct sequencing genomic DNA Results CDl8 expression level on thls patient's leukocyte su rface was signlficantly decreased with normal level ln control g roup hls father and mother Gene analysis revealed that thls patient had a homozygous 899A>T missense mutation ln exon 8 of CDl 8 gene causlng the substitution of Asp to Val at the 300 amlno acid Hls parents were both heterOzvaOus carriers whlle no such mutation was found ln 50 normal controls Conclusion This study disclosed a novel point mutation Asp 300 Val located in a highly conserved region(HCR)of CD18 and confirmed the heteroqeneltv of the mutations causlnq LAD-1, lndlcating lt was qulte beneficial to establish correct and early dlagnosis ln chlIdren with severe LAD-1.
Leukocyte adhesion deficiency, primary immunodeficiency disease, missense mutation, molecular analysis
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陈同辛, Pamela P. W. Lee·Tong-Xin Chen·Li-Ping Jiang·Koon-Wing Chan·Wanling Yang·Bee-Wah Lee·Wen-Chin Chiang·Xiang-Yuan Chen·SusannaE S.Fok·Tsz-LeungLee·MarcoH.ICHo·xi-Qiang Yang·Yu-Lung Lau
J Clin Immunol (2010)30: 121-131,-0001,():
-1年11月30日
Introduction x-linked agarnmagobulinemia(XLA)1s a primary immunodeficiency disorder caused by Bruton’ s tyrosine kLnase (Btk) gene mutation Recent studies suggested genotype-phenotype correlation in XLA. but a definitiveassociation remains controversial. Patients and Methods We examined the relationship between specific Btk gene mutations and sevefitv of clmlcal presentation in 62 Patients with XLA Disease severity was assessed bythe age 0fdisease onsetandthe presence of severe infections, while mutations were classified into severe and mild based on structural and functional consequence by bininformatics analvsis Results Fifty six Btk mutations were identified in 62 patients from 57 kindreds Variation in phenotypes was observed, and there was a tendency of association between genotype and age of disease onset as well as occurrence of severe infections. Conclusion A critical analysis of the circumstances upon presentation also revealed that under-recognition of recurrent infections and relevant thmily history are important hurdles to timely diagnosis of XLA.
X-linked agarnmaglobulinemia XLA, Btk mutations, genotype-phenotype correlation Chinese
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陈同辛, QIAN Ji-hong, ZHU Jian-xing, ZHU Xiao-dong and CHEN Tong-xin
Chinese Medical Journal 2009; 122(16): 1877-1887,-0001,():
-1年11月30日
Background Hypogammaglobulinemia is common in infant humoral lmmunOdeficlencles and has complicated causes and outcomes We almed to determlRe the clinlcal manlfestations lmmunologlcal changes and outcomes of Shanghal lnfants with hypogammaglobulinemia Methods Patients under 2 years old, having one or more warning signs of primary immunodeficiency disorders, serum immunoglobulin levels below the lower llmlt of reference range per age and with normal numbers for lymphocyte subsets were analyzed and followed uP for 2 to 3 years Results A total of 91 chlld ren fmale-to-female ratio: 2.25: 1) pa rticlpated in the study. 1nltial clinlcal presentation was recu rrent upper respiratory tract infection(46%), invasive infection(3%), atopic disease(32%)IgA reduction(77%)was prevalent; 34% patients had more than one isotype reduced Du ring follow-up, 51 of 62 patients(82.25%)had lmmunoglobulins normalized at the age between 12-48 months; these were dlagnosed as transient hypogammaglobulinemia of infancy (THI)Long-term follow-up may reveal a diagnosis for the remaining 11 infants with persistent lower lmmunoglobulin levels who dld not have antlbody titers measu red Earlier onset was correlated with higher rates of normalization More patients were diagnosed with isolated hypogammaglobulinemia in 2006 compared with the prevlous 4 years (2002-2005) Conclusions The awareness of lmmunodeficiency among pedlatricians has been greatly lmproved Recu rrent otitis medla was not a major lnfection ln our patients THl ls a relatively common condltion associated with lnfant hypogammaglobulinemia Inthe absence of specific antibodytiters, the diagnosis ofTHI can be confirmed retrOspectlvely with lg levels normalized ln follow-uP vlsits Therefore long-term follow-up and frequent re-evaluation of these patients are necessary to dlstingulsh them frOm true prlmary lmmunodeficiency
hpyogammaglobulinemia, immunoglobulin normalization, infant, primary immune deficiency, transient hpyogammaglobulinemia of infancy, follow-up
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陈同辛, YOSHIHIRO KOMADA, YAN-WEN ZHou, XAO-LI ZHANG, TONG-XIN CHEN, SHIGEKI TANAKA, EIIcHI AzuMA
British Journal of Haematology, 1997, 96, 147-157,-0001,():
-1年11月30日
Sulnlnarg. AD0ptotic cell death is induced by the cross-linking of Fas/APO-1 receptor fCD951 in acute myefogenous leukaemia fAMLl cells Since CD95 ligand fCD95L1 isexpressed on interleukin-2 fIL-21-activated T cells we Investigated the involvenlent of CD95-CD95L pathway in T cell-mediated cytotoxicitv against AML cells Activated CD8+ T cells could efficlently kfll a parental CD95-sensitlye AML cell line MML-1 and to a lesser extent a CD95-resistant clone MML-1R Neither MML-1 nor MML-1R cells were killed by activated CD4+ T cells The blocking monocfonal antibodv (MoAb) against CD95. ZB4. caused a significant inhibition of T-cell-mediated cvtotoxicitv against M1VEL-1 cells but not against MML-1R cells hlteresttngly, MML-1 cells underwent the classic nuclear morphologic changes and DNA fragmentation characteristic of apoptOSiS when culturedwith activatedTcells Enmneratinn ofapoptoticand necrotic nuclei showed that both apoptOSiS and necrosis were induced in MML-1 cells whereas necrosis was exclusively observed in MML-1R cells Apoptosis of MML-1 cells was completely blocked in the presence of ZB4 MoAb Similarly, blocking by ZB4 MoAb significantly Inhibited T-cell-mediated lysis of flesh AML cells in a CD95-sensitive group, but not in a CD95-refractory group In addition CD8+ T cells expressed CD95L 111RNA more abundantly than CD4+ T cells upon activation bv IL-2 These findings suggest that T-cell-mediated cytotoxicity against AML cells requires participation of CD95-CD95L pathway for cytotoxic signal transduction leading to target apoptosis.
apoptOSIS,, CD95,, CD95 ligand,, activated T cells,, acute myelogenous leukaemia
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【期刊论文】Morphology and ontogeny of dendritic cells in rats at different development periods
陈同辛, Yi-Jun Zhou, Juan Gao, Hua-Mei Yang, ian-Xin Zhu, Tong-Xin Chen, Zhen-Juan He, Yi-Jun Zhou, Hua-Mel Yang, ]lan-Xln Zhu,
World J Gastroenterol 2009 March 14; 15(10): 1246-1253,-0001,():
-1年11月30日
AIM: To study the mo rphology and ontogeny of dend ritic celIs of Peyer's patches in rats at dlfferent development periods. METHoDS: The mo rphometric and flow cytometric analyses were performed to detect a the parameters of villous-crypts axis and the number of OX62+DC, OX62+CD4+SIRP+DC,and OX62+CD4 SIRP DC in the small intestine in different g roups of rats. The relationship between the Darameters of villous-axis and the number of DC and DC subtype were analyzed. RESULTS: All mo rPhometric Parameters changed significantly with the develoPment of PuPs in the diffe rent age g roups(F=10.751, 12.374, 16.527, 5.291, 3.486; P=0.000, 0.000, 0.000, 0.001, 0.015). Villous height levels were unstable and increased from 115.24μm to 140.43μm as ea rly as 3 wk postpartum. Villous a rea inc reased significantly between 5 and 7 wk postpa rtum. peeked uP t0 13817. 60μm2 at 7 wk postpa rtum. ViIious height and c rypt depth ratios were relatively stable and inc reased significantly from 2.80±1.01 to 4.54 4-1.56, 9-11 wk Dostpartum. TheexP ression of OX62+DC inc reased from 33.30%4-5.80%t0 80%±17.30%, 3-11 wk postpa rtum(F=5.536, P=0.0013). 0X62+CD4+SIRP+DC subset levels detected in single-cell suspensions of rat tota Peyer's patch dend ritic cells(PP-DCs)increased significantly from 30.73%±5.16%t0 35.50%±4.08%. 5-7 wk postpartum and from 34.20%±1.35% to 43.60%±2.07%9-11 wk postpa rtum(F=7.216, P=0.005). CoNCLUSIoN: This studY conf rms the age-related changes in villous-c rypt axis dlfferentiation in the sma intestine. Simultaneously,there a re also development and matu ration in rat PP-DCs phenotypic exp ression. Furthermo re, the mo rphologica changes of intestinal mucosa and the development of immune cells(especially DC)peaked at 9-11 wk postpa rtum, indicatina that the intestina mucosae reached a relatively mature state at 11 wk ostpa rtum.
IntestinaI mucosa, Dend ric ceIl, Peye r', s patches, Intestinal development
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【期刊论文】EB病毒对脐带血单核细胞来源树突状细胞凋亡的影响
陈同辛, 金莹莹, 王玺,
上海交通大学学报(医学版),2010,30(2):121~124,-0001,():
-1年11月30日
目的观察EB病毒(EBV)感染对新生儿脐带血单核细胞来源的树突状细胞(DC)凋亡的影响。方法用重组人粒细胞-巨噬细胞集落刺激因子(rhGM-CSF)(50ng/mL)和重组人白细胞介素-4(rhlL-4)(10ng/mL)诱导脐带血单核细胞向DC分化,将DC分为3组:①4G组:细胞分离当天单独加入rhGM-CSF和rhlL-4;②4G+0d EBV组:细胞分离当天同时加入rhGM-CSF、rhlL-4和B95.8细胞上清;③4G+5d EBV组:细胞分离当天加入rhGM-CSF和rhIL-4,培养至第5天后加入B95.8细胞上清。Annexin V-FITC和PI染色流式细胞术检测DC的凋亡百分比;Western Blot检测X-连锁凋亡抑制蛋白(XIAP)的表达。结果培养至第6-14天,4G+0d EBV组DC凋亡百分比明显高于4G组(P<0.05);第7-14天,4G+5d EBV组DC凋亡百分比明显高于4G组(P<0.05)。EBV在不同感染时间点感染DC,对DC凋亡百分比的影响不同。经EBV感染的DC,XIAP的表达明显降低。结论EBV感染促进了脐带血单核细胞来源DC的凋亡,这种促进作用与DC的分化成熟状态有关。
新生儿, 脐带血, EB病毒, 树突状细胞, 凋亡
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陈同辛, 陈娜, 朱亚忠
上海交通大学学报(医学版),2010,30(2):143~146,-0001,():
-1年11月30日
目的确探讨14种食物过敏原特异性IgG检测的I临床意义。方法采用酶联免疫吸附法检测211例过敏性疾病患者血清中14种食物过敏原特异性lgG,并在不同年龄段、不同性别、不同疾病患者间比较各种食物IgG阳性率。结果211例患者中,食物过敏原特异性ISG阳性者193例(91.4%)。14种食物中,鸡蛋的特异性lgG阳性率(73.9%)最高,其次为牛奶(70.6%);未见鸡肉和猪肉的特异性IgG升高。0-12个月年龄组患者以牛奶为首要过敏原,其余年龄组均以鸡蛋为首要过敏原。牛奶特异性IgG阳性率在各年龄组问比较,差异有统计学意义(P<0.01)。食物过敏原特异性IgG阳性率在男女患者间比较,差异无统计学意义(P>0.05)。湿疹患者食物过敏原特异性IgG阳性率(96.4%)最高,慢性腹泻患者阳性率(83.3%)最低;但各种疾病间比较,差异无统计学意义(P>0.05);牛奶特异性lgG阳性率在各种疾病间比较,差异有统计学意义(P<0.01);过敏性紫癜患者对12种过敏原(鸡肉和猪肉除外)均显示阳性反应。结论食物不耐受是一种复杂的变态反应,血清食物过敏原特异性IgG的检测对过敏性疾病的病因诊断具有重要的指导意义。
食物不耐受, 过敏原特异性IgG, 过敏性疾病
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陈同辛, 杨珍, 周纬
临床儿科杂志,2099,27(5):458~461,-0001,():
-1年11月30日
目的探讨上海地区特应质儿童中食物过敏的发病情况和临床特点,以及食物过敏与其他过敏性疾病的关系。方法采用国际上经典的食物过敏诊断方法进行研究,对2007年7月-2008年7月因过敏症状在上海儿童医学中心过敏/免疫专科就诊的720例2个月-17岁特应质儿童,进行病史收集、皮肤点刺试验、排除性饮食试验和开放式食物激发试验。结果最后确诊食物过敏59例(发生率8.19%)。初次发生食物过敏的平均年龄为(0.40±0.33)岁。引起过敏的主要食物为鸡蛋(5.83%)、牛奶(2.78%),虾(1.67%)、鱼(1.25%);3岁以下儿童对鸡蛋过敏多见,≥3岁对虾过敏多见(P<0.01);94.92%的儿童对一种或二种食物过敏。食物过敏100%可引起皮肤症状,25%的特应质儿童因食物过敏引起湿疹;消化道症状发生率为3.39%;呼吸道症状发生率为1.69%;过敏性休克发生率为1.69%。≥3岁仍食物过敏的儿童吸人性过敏原阳性、患哮喘和过敏性鼻炎/2膜炎的人数均显著多于3岁以下的食物过敏儿童(P<0.01)。结论上海地区的特应质儿童中,食物过敏的发生率高,发生年龄小。对有皮肤过敏症状的儿童,应首先考虑食物过敏可能。早期诊断并阻断食物过敏,可预防其他严重过敏性疾病的发生。
食物过敏, 特应质, 儿童
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陈同辛, 曹睿明, 王玺, 朱亚忠, 金莹莹
中华检验医学杂志,2008,31(5):509 ~512,-0001,():
-1年11月30日
目的建立流式细胞术诊断x连锁高IgM血症(X-linked hyper-lgM syndrome,XHIM)的方法。方法取患者、患者母亲和健康对照者外周血,肝素抗凝,用RPML1640培养基稀释,以15μl的佛波醇酯(1ng/μl)和6μl的离子霉素(50ng/μl)作刺激剂,从而将外周m标本分为刺激组和非刺激组,运用荧光标记的抗细胞表面抗原单抗检测CD3+CD8-T淋巴细胞表而CD40配体的表达情况,同时检测CD3+淋巴细胞表面的CD69分子作为激活对照,以确定细胞活化的程度。结果 刺激组细胞表面CD69的表达率均在96%以上;患者母亲及健康对照者CD3+CD8-T淋巴细胞表面CD40L的表达率分别为60.04%和62.87%,而患者仅为0.8%。非刺激组CD69的表达率均小于3%;患者、患者母亲和健康对照者CD40L的表达率分别为0.88%、4.15%和5.51%。结论 流式细胞术可以快速诊断X连锁高IgM血症,有可能成为该类疾病的初筛方法。
高丙种球蛋白血症, 免疫球蛋白M, 流式细胞术
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陈同辛, 杨珍, , 周纬
实用儿科临床杂志,2008,23(9):660-662,-0001,():
-1年11月30日
目的探讨上海地区变应性疾病患儿真菌变应原反应的临床特点。方法采用国际标准化的变应原试剂,对2002年 1月-2007年11月788例变应性疾病患儿行皮肤点刺试验(SPT),检测混合真菌Ⅰ(室外混合真菌)和混合真菌Ⅱ(室内混合真菌)的反应性。并比较真菌Ⅰ和Ⅱ阳性情况在性别、年龄、季节分布和临床表现方面的差异。应用SAS 8.0软件进行统计学处理。结果真菌变应原SPT呈阳性反应者99例。男68例,女3l例;年龄(5.12±2.66)岁;真菌I阳性者83例,真菌Ⅱ阳件者6例,真菌l和Ⅱ均阳性者10例;真菌Ⅰ的反应率(11.80%)显著高于真菌Ⅱ(1.02%)(P<0.01);真菌变应原SPT反应率和性别无明显关系(P>0.05);在≤3岁患儿中反应率(19.98%)高于>3岁者(10.88%)(P<0.01);真菌变应原反应率和季节分布无明显关系(P>0.05);真菌Ⅱ引起的皮肤过敏症状的反应率(6.81%)高于呼吸道过敏症状的反应率(2.14%)(P<0.05);真菌Ⅰ引起的呼吸道过敏症状的反应率(11.86%)和皮肤过敏症状的反应率(14.77%)无明显差异(P>0.05)。结论上海地区变应性疾病患儿中,真菌变应原SPT呈阳性反应者占12.56%,以室外真菌为主。季节分布无显著差异,在≤3岁的儿童中真菌引起的过敏应受到重视,室内真菌可引起更多的皮肤过敏症状。环境控制对预防真菌过敏和改善过敏症状很重要。
真茵, 超敏反应, 变应原
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