王红宁
个性化签名
- 姓名:王红宁
- 目前身份:
- 担任导师情况:
- 学位:
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学术头衔:
博士生导师, 享受国务院特殊津贴专家
- 职称:-
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学科领域:
遗传学
- 研究兴趣:
王红宁,女,博士、教授、博士生导师,四川省西昌市人,1963年8月31日出生;1985年7月获四川农业大学预防兽医学硕士学位,2001年6月 获四川大学遗传学专业现代遗传与生物工程方向博士学位;1996年破格晋升教授。
1992年赴西班牙巴塞罗那大学学习微生物生态学,1999年在美国Iowa大学学习瘦肉型猪规模化养殖技术,2001年在台湾大学、台湾农业科学研究院学习农业生物技术与产业化,2002年赴欧洲学习高新技术产业化与管理,2004年月赴澳大利亚学习国家可持续发展建设与管理;2004年至今,四川大学生命科学学院遗传学专业、微生物学专业博士生导师,2005年至今,四川大学“985”西南资源环境与灾害防治科技创新平台学术带头人。
主要学术职务:
现任中国农业部饲料评审委员会委员,中国畜牧兽医学会禽病学分会副秘书长,中国畜牧兽医学会动物传染病学分会常务理事,四川省畜牧兽医学会副理事长,四川省畜牧兽医学会动物预防医学专业委员会理事长等职。
主要学术成就:
先后主持国家“十五”863项目1项、国家“十五”科技攻关子课题3项、国家重大疫病专项2项,主持部、省级科研项目5项。主持的“ 猪、鸡病原菌耐药性检测及安全高效新兽药研发”获得2004年四川省科技进步一等奖。主持的“禽传染性支气管炎(新变型)综合防治”获得2000年四川省科技进步一等奖。主研的“瘦肉型猪规模化养殖及产业化示范”获得2001年四川省科技进步一等奖,主研的“荥经长毛兔选育配套技术研究应用”已入选2006年四川省科技进步一等奖。共获省部级科技进步一等奖三项(主持二项,主研一项)、2006年新入选一项;二等奖二项(完成者)、三等奖二项(主持一项,主研一项)。以第一发明人申请国家发明专利9项,已公开2项。主编专著3部,副主编专著4部,参编专著6部,协编全国高校教材3部。以第一作者及通讯作者在国内外正式刊物上共发表研究论文116篇,其中发表在SCI、EI收录、一级学术刊物、核心学术期刊56篇。现已招收硕士研究生75人,博士生20人,毕业46人。
荣誉称号:
获国务院政府特殊津贴、四川省学术技术带头人、教育部霍英东教育基金会全国高等院校优秀青年教师奖、四川省优秀青年教师标兵、四川省首届优秀科技工作者、中国共产党十六大代表、四川省十大杰出青年等荣誉。
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主页访问
1920
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关注数
0
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成果阅读
404
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成果数
7
王红宁, Huan Zhang , , Chi Cheng, Min Zheng , Jian-Lin Chen, Ming Jie Meng, Zhong-Zhong Zhao, Qian Chen, Zhao Xie, Jiang Ling Li, Yi Yang, Yi Shen, Hong-Ning Wang, Ze-Zhou Wang*, Rong Gao*
Vaccine 25(2007)7094-7101,-0001,():
-1年11月30日
Experiments were conducted to investigate the effect of a fusion gene of porcine IL-4 and IL-6 (PIL4/IL6) packaged with chitosan nanoparticles (CNPs) in terms of the development of a novel effective adjuvant. The IL4/PIL6 fusion gene was constructed and inserted into a eukaryotic expression vector. The plasmid was bound to CNP and then utilized to orally inoculate 21-day-old female Kunming mice that simultaneously received intramuscular injection of inactivated Escherichia coli vaccine. At 35 days post-vaccination, the mice were challenged by oral feeding with virulent O139: K88 strain EPEC E. coli bacteria. Compared with those of control mice, the content of immunoglobulins and specific antibodies to E. coli increased significantly in the sera of mice immunized with VPIL4/IL6-CNP (P<0.05). Furthermore, the levels of IL-2, IL-4 and IL-6 increased remarkably in the sera of immunized mice (P<0.05). After challenge, these immunological markers were elevated to different degrees in the mice immunized with the fusion gene construct (IL4/VPIL6-CNP) or individual plasmids (VPIL4+VPIL6-CNP). The immunized mice all survived the challenge and did not show any symptoms or lesion, whereas the VR1020-CNP control mice manifested obvious clinical symptoms and hemorrhagic lesions in the digestive tracts. These results demonstrated that VPIL4/IL6 entrapped with CNP is a novel promising adjuvant to promote specific immunity and resistance of animals against infectious pathogen.
Porcine IL4/, IL6 fusion gene, Immunity, Mouse
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王红宁, Zhao Xie , Hui Li , , Jianlin Chen, Hua-bing Zhang, Ying-Yu Wang, Qian Chen, Zhong-Zhong Zhao, Chi Cheng, Huan Zhang , Yi Yang, Hong-Ning Wang*, Rong Gao*
Vaccine 25(2007)8163-8171,-0001,():
-1年11月30日
In order to explore the safe and effective adjuvant for promotion of immunity of animals against infection, the experiment was carried out to shuffle Tibet pig IL-2 cDNAwith other IL-2 cDNAfrom human, yak and mouse, and the effect of shuffled IL-2 (IL-2S) gene in vivowas investigated on immunity of mice to Pasteurella multocida. The IL-2S protein was found to remarkably promote the proliferation of pig lymphoblasts than the native pig IL-2 protein. Then the IL-2S gene was cloned into VR1020 eukaryotic plasmid (VRIL2S) and enwrapped with chitosan nanoparticles (CNP-VRIL2S). Twenty-one day old female Kunming mice were muscularly inoculated respectively with the CNP-VRIL2, CNP-VRIL2S and CNP-VR1020 along with Pasteurella multocida vaccine, and orally challenged with virulent Pasteurella multocida on 28 days post-vaccination. The blood was weekly collected to detect the change of IgG, IgA, IgM, specific antibody, IL-2, IL-4 and IL-6 by ELISA. The immunoglobulins, specific antibody and interleukins significantly increased in CNP-VRIL2S group compared with the control mice after vaccination and challenge, and 9 of 10 immunized mice survived challenge, while the all control mice manifested severe symptoms and lesions, and finally died of infection. These indicated that VRIL2S entrapped with CNP is a novel safe and effective adjuvant to boost the specific immunity and resistance of animal against infectious pathogen, which could facilitate the development of highly promising powerful adjuvant.
Pig interleukin-2 gene, DNA shuffling, Mouse, Immunity, Pasteurella multocida
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【期刊论文】The immunoreactivity of a chimeric multi-epitope DNA vaccine against IBV in chickens
王红宁, Lang Tian b, Hong-ning Wang a, *, Dan Lu b, Yun-fei Zhang b, Ting Wangb, Run-ming Kang b
Biochemical and Biophysical Research Communications 377(2008)221-225,-0001,():
-1年11月30日
Epitope-based vaccines designed to induce cellular immune response and antibody responses specific for infectious bronchitis virus (IBV) are being developed as a means for increasing vaccine potency. In this study, we selected seven epitopes from the spike (S1), spike (S2), and nucleocapsid (N) protein and constructed a multi-epitope DNA vaccine. The 7-day-old chickens were immunized intramuscularly with multi-epitope DNA vaccine encapsulated by liposome and boosted two weeks later, and were challenged by virulent IBV strain five weeks post booster. The results showed that multi-epitope DNA vaccine led to a dramatic augmentation of humoral and cellular responses, and provided up to 80.0% rate of immune protection. The novel immunogenic chimeric multi-epitope DNA vaccine revealed in this study provided a new candidate target for IBV vaccine development.
Infectious bronchitis virus (, IBV), , Multi-epitope, Chimeric gene, DNA vaccine, Immunogenicity
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王红宁, An-Yun Zhang, Hong-NingWang*, Guo-Bao Tian, Yi Zhang, Xin Yang, Qing-Qing Xia, Jun-Ni Tang, Li-Kou Zou
International Journal of Antimicrobial Agents 33(2009)456-460,-0001,():
-1年11月30日
To study the prevalence of antimicrobial resistance in faecal bacteria from Giant pandas in China, 59 isolates were recovered from faecal pats of 30 Giant pandas. Antimicrobial susceptibility testing of the isolateswas performed by the standardised disk diffusion method (Kirby-Bauer). Of the 59 study isolates, 32.20% were resistant to at least one antimicrobial and 16.95% showed multidrug-resistant phenotypes. Thirteen drug resistance genes [aph(3')-IIa, aac(6')-Ib, ant(3")-Ia, aac(3)-IIa, sul1, sul2, sul3, tetA, tetC, tetM, cat1, floR and cmlA] were analysed using four primer sets by multiplex polymerase chain reaction (PCR). The detection frequency of the aph(3')-IIa gene was the highest (10.17%), followed by cmlA (8.47%). The genes aac(6')-Ib, sul2 and tetAwere not detected. PCR productswere confirmed by DNA sequence analysis. The results revealed that multidrug resistancewas widely present in bacteria isolated from Giant pandas.
Giant panda, Antimicrobial resistance, Resistance genes, Multiplex PCR
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王红宁, Jun-ni Tang, Zhi-guang Zeng, Hong-ning Wang *, Tai Yang, Peng-ju Zhang, Yu-ling Li, An-yun Zhang, Wen-qiao Fan, Yi Zhang, Xin Yang, Su-jun Zhao, Guo-bao Tian, Li-kou Zou
Journal of Microbiological Methods 75(2008)432-436,-0001,():
-1年11月30日
Polymerase chain reaction (PCR) detection of microorganism in faecal specimens is hampered by poor recovery of DNA and by the presence of PCR inhibitors. In this paper, we describe a new modified method for extracting PCR-quality microbial community DNA from pig faecal samples, which combines the pretreatment with polyformaldehyde, and subsequent DNA lysis in the presence of CTAB, salt, PVP, and β-mercaptoethanol, followed by isolation of nucleic acids using chloroform (no phenol) based protocol. The method resulted in a 1.3- to 11-fold increase in DNA yield when compared to four other widely used methods. Genomic DNA extracted from all five methods was assessed by both agarose gel electrophoresis and polymerase chain reaction for amplification of 16S rDNA specific fragments. The results showed that the improved method represented a reproducible, simple, and rapid technique for routine DNA extraction from faecal specimens and was notably better than using the QIAamp® DNA Stool Mini Kit.
Pig faeces, DNA extraction, β-mercaptoethanol, Comparison, PCR
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王红宁, Yi Yang, Jianlin Chen, Hui Li, Yingyi Wang, Zhao Xie, Mei Wu, Huan Zhang, Zhongzhong Zhao, Qian Chen, Manliang Fu, Kaiyuan Wu, Cheng Chi, Hongning Wang*, Rong Gao*
Comparative Immunology, Microbiology & Infectious Diseases 30(2007)19-32,-0001,():
-1年11月30日
Interleukin-2 (IL-2) is vital to elicit and amplify the cellular and humoral immune responses to foreign antigens, which is extensively utilized in the control of infectious disease and treatment of various cancers. Porcine and murine IL-2 genes were, respectively, subcloned into VR1020, designated as VPIL-2 and VMIL-2, and then encapsulated in chitosan nanoparticles (CNP) prepared by ionic linkage. The BALB/c mice were intramuscularly co-administrated with chitosan-IL-2 nanoparticles (CNP-IL2) and paratyphoid vaccine to test the adjuvant effect of CNP-IL2. On day 35, the immunized mice were orally challenged with virulent Salmonella. The content of IgG, IgA, IgM, IL-2, IL-4, IL-6 and specific antibody titer as well as the number of immunocompetent cells were systematically analyzed in the vaccinated mice. The results revealed that the levels of immunoglobulins, cytokines, the specific antibodies, together with the numbers of lymphocytes significantly increased in vaccinated mice inoculated with CNP-VPIL2 in contrast with those with naked IL-2 plasmids and blank plasmids. The CNP-VPIL2 immunized mice exhibited higher humoral and cellular immune responses, less severe clinical signs and lesions of disease caused by the bacteria than the other groups after challenge. These findings suggest that CNP-VPIL2 has a significant enhancement effect on immune responses of mice, which results in better immunoprotection against Salmonella infection, indicating that CNP-VPIL2 could be employed as an effective immunoadjuvant to elevate immunity of animals to conventional vaccines.
Porcine IL-2 gene, Mice, Immune response, Chitosan nanoparticle, Salmonella
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王红宁, Mengjun Tang b, Hongning Wang a, *, Sheng Zhou b, Guobao Tian a
Journal of Virological Methods 149(2008)42-48,-0001,():
-1年11月30日
A DNA vaccine against infectious bronchitis virus (IBV) can induce specific humoral and cell-ediated immunity. However, compared to conventional vaccines, DNA vaccines usually induce poor antibody responses. To develop a more potent IBV DNA vaccine formulations, a monocistronic vector encoding the nucleocapsid protein of IBV and a bicistronic vector separately encoding the nucleocapsid protein and immunestimulatory interleukin-2 were constructed. When the DNA vaccines were administered to the quadriceps muscle of chickens, the induced humoral and cellular responses were evaluated. There was a significant difference in ELISA antibody levels elicited by either monocistronic or bicistronic DNA vaccines. The percentage of CD3+, CD3+CD8+ and CD3+CD4+ subgroups of peripheral blood T-lymphocytes in chickens immunized with bicistronic DNA vaccine were higher than those in chickens immunized with monocistronic DNA vaccine. When chickens were challenged with a virulent strain of IBV, the protective efficacy could be enhanced significantly after immunization with bicistronic DNA vaccine. These results demonstrated that bicistronic DNA vaccine is an effective approach to increase IBV DNA vaccine immunogenicity.
Infectious bronchitis virus, DNA vaccine, Nucleocapsid protein, Bicistronic plasmid
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