马万云
从事单分子探测及应用研究。目前研究重点:1、单分子探测与成像技术研究(双光子激发荧光显微成像、全内反射衰逝波激发荧光成像、原子力显微镜等);2、单分子层次上生物大分子之间相互作用的动力学过程;3、活细胞内特定生物大分子的高灵敏、实时、原位四维动态观测及在生物医学中的应用;4、活细胞纳米生物力学研究;5、生物材料的超微结构及性能;6、高灵敏探测在资源、环境、材料、生物医学中的应用。
个性化签名
- 姓名:马万云
- 目前身份:
- 担任导师情况:
- 学位:
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学术头衔:
博士生导师
- 职称:-
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学科领域:
原子分子物理学
- 研究兴趣:从事单分子探测及应用研究。目前研究重点:1、单分子探测与成像技术研究(双光子激发荧光显微成像、全内反射衰逝波激发荧光成像、原子力显微镜等);2、单分子层次上生物大分子之间相互作用的动力学过程;3、活细胞内特定生物大分子的高灵敏、实时、原位四维动态观测及在生物医学中的应用;4、活细胞纳米生物力学研究;5、生物材料的超微结构及性能;6、高灵敏探测在资源、环境、材料、生物医学中的应用。
马万云
教授
学历:
清华大学工程物理系理学学士并获优秀毕业生奖章(1982年);
清华大学物理系理学博士(1987年)。
工作经历:
清华大学物理系讲师(1987-1991),副教授(1991-1996),教授(1996-现在),博导(1998-现在);
清华大学“原子分子纳米科学”教育部重点实验室,副主任(1994-2001),主任(2001-2006);
清华大学“原子分子纳米科学研究中心” 副主任(1996-现在);
意大利Parma大学物理科学研究所客座教授(1992.8-1994.2;1995.7-9;1998.9-12;2000.10-2001.1;2007.7-9);
香港浸会大学客座教授(2002.7-12)。
教学
主讲本科生《大学物理》(力学、电磁学、热学、光学、量子物理)
研究领域
从事单分子探测及应用研究。单分子探测是指对灵敏区内目标分子的探测灵敏度达到单个分子量级。这是一项意义重大的基础研究。为在单分子层次上研究酶催化反应动力学、生物大分子的结构与功能及相互作用、细胞信号传导过程、复杂体系中超痕量物质分析等方面提供重要的研究手段。在生物医学、材料科学、资源环境等领域有广阔的应用前景。目前研究重点:
1、单分子探测与成像技术研究(双光子激发荧光显微成像、全内反射衰逝波激发荧光成像、原子力显微镜等)
2、单分子层次上生物大分子之间相互作用的动力学过程;
3、活细胞内特定生物大分子的高灵敏、实时、原位四维动态观测及在生物医学中的应用;
4、活细胞纳米生物力学研究;
5、生物材料的超微结构及性能;
6、高灵敏探测在资源、环境、材料、生物医学中的应用
奖励、荣誉
1、 “原子(分子)层次的超灵敏谱学(及应用)与原子分子高激发态谱学研究”,国家教委科技进步一等奖 (1995年)
2、 “激光单原子探测超灵敏谱学及其应用研究”,国家教委科技进步二等奖(1991年)
3、 “极细微尺度(单个原子、分子直至纳米粒子)超灵敏谱学及其应用”,教育部科技进步二等奖 (1999年)
4、 “原子分子测控的谱学基础研究”,北京市科学技术二等奖(2002年)
5、 “胸腺细胞凋亡的亚硝酰转化机制及其在中药药理上的应用”,中国中医科学院中医药科学技术进步二等奖(2007年)
6、 清华大学首届"学术新人"奖(1995年)
现任学术兼职
中国物理学会原子分子物理专业委员会委员
中国物理学会光物理专业委员会委员
中国物理学会女物理工作者委员会委员
《光谱学与光谱分析》编委
Member of the International Referee Board of《Acta BioMedica》
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主页访问
1333
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关注数
0
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成果阅读
373
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成果数
9
【期刊论文】MAC mode atomic force microscopy studies of living samples, ranging from cells to fresh tissue
马万云, Guanglu Gea, Dong Hana, b, *, Danying Linb, Weiguo Chua, Yunxu Sunb, Lei Jianga, c, Wanyun Mab, Chen Wanga
Ultramicroscopy 107(2007)299-307,-0001,():
-1年11月30日
Magnetic AC mode (MAC mode) atomic force microscopy (AFM), a novel type of tapping mode AFM in which the cantilever is driven directly by a magnetic field, is a powerful tool for imaging with high spatial resolution and better signal-to-noise in liquid environment. It may largely extend the application of AFM to living samples, especially those are sensitive to cantilever forces, even to multilayer tissue samples. However, there are few reports on the imaging of living cells by MAC mode AFM previously. In our present study, we explore the optimal imaging conditions of MAC mode AFM on living astrocytes and fresh arterial intima surface. We also used nude tips for PicoTREC panel (i.e., Aux in BNC, a new data collecting channel) to image living samples and discussed its difference with phase imaging. We show that living biological samples can be imaged by MAC mode AFM at details of comparable resolution as those by high resolution scanning electron microscopy. Furthermore, the combination of height, amplitude, phase and TREC panel signals provide abundant informations for the characteristics of living samples, such as topography, profile, stiffness and adhesion.
Atomic force microscopy, Magnetic AC mode, Astrocytes, Arterial Intima, Field emission scanning electron microscopy
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马万云, Dongqiang Han, , Wanyun Ma, &, Dieyan Chen
Chromatographia 2007, 66, December,-0001,():
-1年11月30日
Benzene, toluene, ethylbenzene, and xylenes (BTEX) are commonly found in crude oil and are used in geochemical investigations as direct indicators of the presence of oil and gas. BTEX are easily volatile and can be degraded by microorganisms, which affect their precise measurement seriously. A method for determining the biodegradation process of BTEX in seabed sediment using dynamic headspace (purge and trap) gas chromatography with a photoionization detector (PID) was developed, which had a detection limit of 7.3-13.2 ng L-1 and a recovery rate of 91.6-95.0%. The decrease in the concentration of BTEX components was monitored in seabed sediment samples, which was caused by microorganism biodegradation. The results of BTEX biodegradation process were of great significance in the collection, transportation, preservation, and measurement of seabed sediment amples in the geochemical investigations of oil and gas.
Gas chromatography Purge and trap method Biodegradation Seabed sediment BTEX
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【期刊论文】Sensitive elemental analysis of aqueous colloids by laser-induced plasma spectroscopy
马万云, X. Y. Pu, a) W. Y. Ma, b) and N. H. Cheungc)
APPLIED PHYSICS LETTERS VOLUME 83, NUMBER 16 20 OCTOBER 2003,-0001,():
-1年11月30日
Highly sensitive elemental analysis of lead carbonate colloids was demonstrated by two-pulse laser-induced plasma spectroscopy. The first laser pulse created a vapor plume with the particulates concentrated in space because of their slower propagation. They were then ablated by an ArF laser pulse that efficiently atomized and excited the lead analyte. The lead emissions were much enhanced, while the background continuum interference was minimized. The detection limit for lead was shown to be 14.2 ppb, compared with 13 ppm achieved by conventional laser-induced breakdown spectroscopy of lead ions in water and 210 ppb for lead aerosols.
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马万云, Rongying Wanga, Xiaoning Lub, Wanyun Maa, *
Journal of Chromatography B, 779(2002)157-162,-0001,():
-1年11月30日
A non-competitive immunoassay based on micellar electrokinetic capillary chromatography (MECC) with laser-induced fluorescence (LIF) detection has been developed for the determination of alpha-fetoprotein (AFP). The anti-AFP antibody was labeled with fluorescein isothiocyanate (FITC) and the product was used as a fluorescent tracer, then AFP was mixed with the labeled antibody. After incubation, the immune AFP–antibody complex was separated from labeled free antibody by MECC. The parameters affecting separation such as the concentration of sodium dodecyl sulfate (SDS), the buffer pH and separation voltage were investigated and the following conditions were selected: 20 mM tetraborate containing 100 mM SDS at pH 9.50, and 20 kV separation voltage. The detection limit of this assay was 0.1 ng/ml with a linear range spanning two orders of magnitude. This method was applied to determine AFP in human serum.
a-Fetoprotein
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【期刊论文】Polymerization of dental composite resins using plasma light
马万云, L. Fanoa, W.Y. Mab, P.A. Marcolic, S. Pizzic, V. Fanoa, *
Biomaterials 23(2002)1011-1015,-0001,():
-1年11月30日
Five visible light-cured composite resins used as restoration or adhesive materials in dentistry, were irradiated with high energy plasma light (1300 mW/cm2), and contraction, rate of contraction, irradiation-induced temperature were analysed. A comparison was carried out with the same materials irradiated with a conventional halogen light (400mW/cm2). The exposure to the photoactivating lights was either continuously or sequentially in three or more intervals with 10 min between intervals. Comparing the lengths of exposure of both lights, which induced the same contraction in a given material, it was found that the exposure length to the plasma light was greatly reduced, when compared with the exposure length of the halogen light (1:10). Frequently, the final contraction of plasma-irradiated materials was lower, whereas the rate of contraction, as indicated by the linear dimensional variation curves obtained by laser beam scanning method, did not show significant differences between the two lights. The temperature increase induced by plasma light on the material did not exceed the temperature induced by conventional light.
Composite resins, Restorative dental materials, Dental adhesive, Polymerization
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马万云, L. Fanoa, V. Fanoa, *, W.Y. Mab, G.W. Yangb, F. Zhub
Biomaterials 22-(2001)2353-2358,-0001,():
-1年11月30日
The microstructure of four cements, setting by different mechanisms (acid-base, dual cure, triple cure), was studied. The porosity of unpolymerized materials was detected by stereomicroscopy. After polymerization and storage in water or lactic acid solution, the porosity, filler distribution and gel layer, which was formed at the filler/matrixinterface of polymerized materials, were examined by confocal laser microscopy. For this purpose, the specimens were treated with fluorescent dye solution before the test. The results showed that hydrolytic degradation (pH 7) mainly involved the resin matrix, and the acid erosion (pH 3.5) involved the gel layer too. As regards the filler, materials with different setting mechanism released the glass particles in di!erent times. The loss of the filler particles occurred quicker in acid}base setting cements, and slower in triple-cured material.
Glass-ionomer cements, Restorative materials, Confocal laser microscopy, Stereomicrosopy, Porosity, Gel layer
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马万云, Ma Wan-yun*, Xue Meng, Zhang Jun, Chen Die-yan
Spectrochimica Acta Part B 53(1998)1421-1425,-0001,():
-1年11月30日
Ultratrace gold (Au) in geogas samples has been determined by means of laser excited atomic fluorescence spectrometry combined with graphite electrothermal atomization and time-gate technique. Gold atoms were excited from the ground state to the 6p2P3/2 state by a pulsed laser beam with a wavelength of 242.8 nm. Fluorescence photons with a wavelength of 312.3 nm were measured by a photon-counting unit. The time-gate technique was used to reduce the background radiation caused by the furnace. This method has proved to be highly sensitive with minimal background interference. Eighty-two geogas samples were analysed and the Au contents obtained were in the range of 0.002–0.182 ng l-1. The study of Au concentration of geogas in soil is of great interest in prospecting gold deposits.
Au, Geogas, Graphite furnace, Time-gate, Laser excited atomic fluorescence spectrometry
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【期刊论文】Study of dental materials by laser beam scanning
马万云, V. Fano, * W.Y. Ma, I. Ortalli, K. Pozela
Biomaterials 19(1998)1541-1545,-0001,():
-1年11月30日
Several properties of dental materials are characterized by dimensional variation induced by different parameters. In this work a non-contact method used for measurement of linear dimensional variation is shown. The accuracy is ±1 k for sample length ranging from 1 to 70 mm.
Characterization of dental materials, Dimensional variation, Laser beam scanning
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【期刊论文】Experimental Study of Autoionizing States of AuI
马万云, W.Z. Zhao, X.Y. Xu, W.Y. Ma, Y. Cheng, Q. Hui, K.L. Wen, and D.Y. Chen
Appl. Phys. B 52, 299-304(1991),-0001,():
-1年11月30日
This paper describes the investigation of the autoionizing states of AuI by exciting the states of 6d 21)3/2 and 6d 2D5/2 with tunable dye lasers by means of a resonance ionization time-of-flight mass spectrometer (RI-TOFMS). Many new autoionizing states were found, a few of which have narrow-linewidths of about 0.1 cm -1. The photoionization efficiencies for different channels were compared. Among the autoionizing states found so far, the highest photoionization yield is given by two newly obtained states from the state 6d 2D5/2, 3683.6cm-1 and 4636.5cm-1 above the first ionization limit. In addition, the Shore-Fano profile parameters of several autoionizing resonances were determined by a nonlinear fitting program.
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