朱兴全
主要研究方向是寄生虫的功能基因组学、线粒体基因组学;分子分类学、分子遗传学、分子诊断学;功能性基因的克隆及表达;PCR技术及基因突变检测技术等。
个性化签名
- 姓名:朱兴全
- 目前身份:
- 担任导师情况:
- 学位:
-
学术头衔:
博士生导师, 国家杰出青年科学基金获得者
- 职称:-
-
学科领域:
海洋化学
- 研究兴趣:主要研究方向是寄生虫的功能基因组学、线粒体基因组学;分子分类学、分子遗传学、分子诊断学;功能性基因的克隆及表达;PCR技术及基因突变检测技术等。
男,1963年8月出生,毕业于四川畜牧兽医学院(学士学位)、中国农业科学院研究生院(硕士学位)及澳大利亚墨尔本大学兽医学院(博士学位,PhD)。1988年8月―1995年5月在中国农业科学院兰州兽医研究所任课题主持人,1992年3月破格晋升为副研究员,担任中国农业科学院第三届学术委员会委员。1995年5月-2002年2月在澳大利亚新英格兰大学及墨尔本大学留学、工作。2002年2月起担任华南农业大学兽医寄生虫学研究室主任、教授,博士生导师。2005年元月起被聘为广东省“珠江学者”特聘教授。
近20年来在国内外一直主要从事人畜共患寄生虫病病原的分子生物学、免疫学及防制技术的研究工作,主持承担国际科学基金(IFS)项目、国家杰出青年科学基金项目、国家科技部“攀登计划”项目、国家自然科学基金项目、农业部“七•五”、“八•五”攻关重点项目、教育部“优秀青年教师资助计划项目”、教育部“博士点基金”、教育部“留学回国人员科研基金”、人事部“留学人员科技活动择优资助项目”及广东省自然科学基金重点项目、广东省科技攻关项目等研究课题20多项。荣获2002年度“国家杰出青年科学基金”。
用“双语”主讲兽医专业博士生、硕士生及本科生的《现代寄生虫学进展》、《高级兽医寄生虫学》、《兽医寄生虫学》、《生物技术在兽医学上的应用》、《兽医寄生虫学实验技术》、《预防兽医学硕士研究生专业英语》、《兽医专业英语》等7门核心及专业课程。所讲授的所有课程均深受学生欢迎,教学效果十分理想。现指导博士研究生7人,硕士研究生15人,留学生1人,本科生12人。
兼任国际科学基金委员会(IFS)科学顾问、世界兽医寄生虫学促进会(WAAVP)会员;国家科技部、国家教育部、国家自然科学基金委员会项目评审专家;中国动物学会寄生虫学分会常务理事、中国畜牧兽医学会家畜寄生虫学分会常务理事、广东省寄生虫学会常务理事、广西大学兼职教授、中山大学“热带医学教育部重点实验室”学术委员会委员、中山大学“寄生生物研究中心”学术委员会委员、华南农业大学学术委员会委员、中国农业科学院研究生院研究生论文评审专家、《动物学报》编委、《寄生虫学与医学昆虫学报》编委、《中国兽医寄生病》杂志编委、《热带医学杂志》编委、《华南农业大学学报》编委等30多个学术职务。为广州市政协常委。
先后荣获中共中央组织部、国家人事部及中国科协联合颁发的第四届“中国青年科技奖”(全国每两年评100名);2005年元月起被聘为广东省“珠江学者”特聘教授;广东省“千百十工程”国家级培养对象;农业部科技进步三等奖(3项);中国农学会“第四届青年科技奖”;中国农业科学院首届“十佳青年”称号;中国农业科学院青年优秀论文壹等奖(2次);澳大利亚墨尔本大学“Rowden White”奖;澳大利亚墨尔本大学研究生院“论文写作奖”等各种奖励及荣誉多项。2001年获世界兽医寄生虫学促进会“Peter Nansen青年科学家奖”提名。1992年,作为兽医界的两名代表参加了在北京人民大会堂召开的建国以来最高规格、规模最大的青年学术盛会―“中国科协首届青年学术年会”,并受到党和国家领导人的亲切接见。
在国际上发表英文论文56篇,其中被SCI收录52篇。在国内发表论文170多篇,主编、副主编、参编专著、教材10部。主要研究方向是寄生虫的功能基因组学、线粒体基因组学;分子分类学、分子遗传学、分子诊断学;功能性基因的克隆及表达;PCR技术及基因突变检测技术等。
-
主页访问
3831
-
关注数
0
-
成果阅读
1066
-
成果数
22
朱兴全, Y.B. Weng a, Y.J. Hu b, Y. Li b, B.S. Li b, R.Q. Lin a, D.H. Xie a, R.B. Gasserc, X.Q. Zhu a, *
Veterinary Parasitology 127(2005)333-336,-0001,():
-1年11月30日
The prevalence of intestinal parasites was investigated in intensive pig farms in Guangdong Province, China between July 2000 and July 2002. Faecal samples from 3636 pigs (both sexes and five age groups) from 38 representative intensive pig farms employing different parasite control strategies were examined for the presence of helminth ova and protozoan oocysts, cysts and/or trophozoites using standard techniques. Of the 3636 pigs sampled, 209 (5.7%) were infected with Trichuris suis, 189 (5.2%) with Ascaris, 91 (2.5%) with Oesophagostomum spp., 905 (24.9%) with coccidia (Eimeria spp. and/or Isospora suis) and 1716 (47.2%) with Balantidium coli. These infected pigs were mainly from farms without a strategic anti-parasite treatment regime. Concurrent infection of multiple parasites was common, and T. suis was the most common nematode infecting breeding, young and mature pigs. The results of the present investigation provide relevant 'base-line' data for assessing the ffectiveness of control strategies against intestinal parasitism in intensively raised pigs in Guangdong Province, China.
Ascaris, Balantidium coli, China, Coccidia, Oesophagostomum spp., , Pig, Trichuris suis
-
63浏览
-
0点赞
-
0收藏
-
0分享
-
473下载
-
0评论
-
引用
朱兴全, An-Xing Li a, Xiang-Yun Wu a, Xue-Juan Ding a, Rui-Qing Lin b, Ming-Quan Xie a, Zhao-Rong Lun a, Xing-Quan Zhu b, *
Molecular and Cellular Probes 19(2005)35-39,-0001,():
-1年11月30日
PCR-based single strand conformation polymorphism (SSCP) analysis was used to characterize monogenean specimens of the subfamily Benedeniinae of morphologically uncertain specific status from different marine fish species, using Neobenedenia melleni, N. girellae and Entobdella corona for comparison. The first internal transcribed spacer (ITS-1) and the 50 terminal variable region (D1-D3 domains) of the large subunit ribosomal DNA (lsrDNA) were amplified separately from individual monogeneans, and the amplicons were subjected to PCR-SSCP analyses, followed by direct sequencing. Both SSCP patterns and the ITS-1 sequences data allowed specimens representing Entobdella spp. from the host Taeniura melanospilos to be unequivocally distinguished from those representing Neobenedenia spp. and those representing Benedenia spp. Neobenedenia girellae, a morphologically controversial species, had identical SSCP banding pattern and ITS-1 sequence to that of N. melleni, supporting the proposal that N. girellae is a synonym of N. melleni. Neobenedenia spp. and Benedenia spp. had identical SSCP patterns and ITS-1 sequences. These findings and the PCR-SSCP approach taken should have implications for the accurate identification and assessment of taxonomic validity of other important monogenean groups of the marine fish.
Single strand conformation polymorphism (, SSCP), , Polymerase chain reaction, Large subunit ribosomal DNA (, lsrDNA), , First internal transcribed spacer (, ITS-1), , Benedeniinae
-
61浏览
-
0点赞
-
0收藏
-
0分享
-
397下载
-
0评论
-
引用
【期刊论文】Clonorchiasis: a key foodborne zoonosis in China
朱兴全, Zhao-Rong Lun, Robin B Gasser, De-Hua Lai, An-Xing Li, Xing-Quan Zhu, Xing-Bing Yu, and Yue-Yi Fang
Lancet Infect Dis 2005; 5: 31-41,-0001,():
-1年11月30日
The oriental liverfluke, Clonorchis sinensis, is of major socioeconomic importance in parts of Asia, including China, Japan, Korea, Taiwan, and Vietnam. The parasite is transmitted via snails to freshwater fish, and then to human beings and other piscivorous mammals, and causes substantial clinical or subclinical disease, known as clonorchiasis. There is considerable evidence for an aetiological relation between clonorchiasis and cholangiocarcinoma in human beings. It is estimated that about 35 million people are infected globally, of whom approximately 15 million are in China. Although very little information from China has been published in the English language, recent nalyses of epidemiological data sets suggest that clonorchiasis is having an increased human-health impact due to the greater consumption of raw freshwater fish. To gain an improved insight into clonorchiasis in China, this review provides a background on the parasite and its life cycle, summarises key aspects regarding the pathogenesis, diagnosis, and treatment of clonorchiasis, describes the geographic distribution and prevalence of clonorchiasis, and makes some recommendations for future research and the control of this important disease.
-
52浏览
-
0点赞
-
0收藏
-
0分享
-
145下载
-
0评论
-
引用
朱兴全, Sarah N. Kleeman a, Robert D. Adlard b, *, Xingquan Zhu c, d, Robin B. Gasser c
Molecular and Cellular Probes 18(2004)133-138,-0001,():
-1年11月30日
Marteilia sydneyi (Paramyxea) is the causative agent of QX disease in oysters. In spite of the economic impact of this disease, its origin and the precise reason(s) for its apparent spread in Australian waters are not yet known. Given such knowledge gaps, investigating the population genetic structure(s) of M. sydneyi populations could provide insights into the epidemiolog and ecology of the parasite and could assist in its prevention and control. In this study, single strand conformation polymorphism (SSCP)-based analysis of a region (195 bp) of the first internal transcribed spacer (ITS-1) of ribosomal DNA was employed to investigate genetic variation within and among five populations of M. sydneyi from oysters from five different locations in eastern Australia. The analysis showed the existence of a genetic variant of M. sydneyi common to the Great Sandy Strait, and the Richmond and Georges Rivers, as distinct from variants at the Pimpama and Clarence Rivers. Together with historical and other information relating to the QX disease outbreaks in eastern Australia, the molecular findings support the proposal that the parasite originated in the Great Sandy Strait and/or Richmond River and then extended southward along the coast. From a technical perspective, the study emonstrated the usefulness of SSCP as a tool to study the population genetics and epidemiology of M. sydneyi.
Single strand conformation polymorphism analysis, QX disease, Marteilia sydneyi (, Paramyxea), , Population genetics, Epidemiology
-
63浏览
-
0点赞
-
0收藏
-
0分享
-
135下载
-
0评论
-
引用
【期刊论文】Characterisation of Fasciola species from Mainland China by ITS-2 ribosomal DNA sequence☆
朱兴全, W.Y. Huang a, B. He a, C.R. Wang b, X.Q. Zhu c, *
Veterinary Parasitology 120(2004)75-83,-0001,():
-1年11月30日
Isolates of Fasciola (Platyhelminthes: Trematoda: Digenea) from different host species and geographical locations in Mainland China were characterised genetically. The second internal transcribed spacer (ITS-2) of nuclear ribosomalDNA(rDNA)was amplified from individual trematodes by polymerase chain reaction (PCR), and the representative amplicons were cloned and sequenced. The length of the ITS-2 sequences was 361-362 bp for all Chinese Fasciola specimens sequenced. While there was no variation in length or composition of the ITS-2 sequences among multiple specimens from France, Sichuan and Guangxi, sequence difference of 1.7% (6/362) was detected between specimens from France and Sichuan, and those from Guangxi. Based on ITS-2 sequence data, it was concluded that the Fasciola from Sichuan represented Fasciola hepatica, the one from Guangxi represented Fasciola gigantica and the one from sheep from Heilongjiang may represent an "intermediate genotype", as its ITS-2 sequences were unique in that two different ITS-2 sequences exist in the rDNA array within a single Fasciola worm. One of the sequences is identical to that of F. hepatica, and the other is almost identical to that of F. gigantica in that nucleotides at five of the six polymorphic positions represent F. gigantica. This microheterogeneity is possibly due to sequence polymorphism among copies of the ITS-2 array within the same worm. Based on the sequence differences, a PCR-linked restriction fragment length polymorphism (PCR-RFLP) assay was established for the unequivocal delineation of the Fasciola spp. from Mainland China using restriction endonuclease Hsp92II or RcaI. This assay should provide a valuable tool for the molecular identification and for studying the ecology and population genetic structures of Fasciola spp. frm Mainland China and elsewhere.
Fasciola spp., , PCR, PCR-linked restriction fragment length polymorphism (, PCR-RFLP), , Ribosomal DNA, Second internal transcribed spacer (, ITS-2),
-
63浏览
-
0点赞
-
0收藏
-
0分享
-
461下载
-
0评论
-
引用
朱兴全, Zhao-Rong Lun
Parasitol Res (2004) 92: 335-340,-0001,():
-1年11月30日
A total of 20 random primers (10-mers) were used to amplify RAPD markers from the genomic DNA of four Trypanosoma brucei stocks from East and West Africa, four T. evansi stocks from Africa, Asia and South America and one T. equiperdum stock from Asia. Between 65 and 88 reproducible fragments ranging from 0.25 to 2.15 kb were generated from these stocks depending on the stock/primer combination. The similarity coefficient (SC) among the stocks of T. brucei from Kenya, Nigeria, Tanzania and Zambia ranged from 62.9% to 74.0% (average: 67.6%). The SC among the stocks of T. evansi from Kenya, China and Brazil was 76.4%-95.5% (average: 86.4%), while the SC between T. evansi stock from China and Brazil was 95.5%. For T. evansi and T. equiperdum, the SC among the stocks ranged from 81.2% to 94.4% (average: 87.6%). As for the SC among the stocks of T. brucei and T. evansi, it was found to be from 54.7% to 80.3% (average: 68.0%) and the SC among stocks of T. brucei and T. equiperdum was from 59.4% to 76.9% (average: 68.1%). Our results indicate that the stocks of T. evansi from China and from Brazil are more closely related to the stock of T. equiperdum from China than to the stocks of T. evansi isolated from Kenya and to the stocks of T. brucei. In addition, our results further support the hypothesis that T. evansi stocks from China and Brazil could have arisen from a single lineage. The possible evolution of T. evansi and T. equiperdum is also discussed.
-
43浏览
-
0点赞
-
0收藏
-
0分享
-
71下载
-
0评论
-
引用
【期刊论文】PCR amplification and sequencing of ITS1 rDNA of Culicoides arakawae ☆
朱兴全, G.Q. Li*, Y.L. Hu, S. Kanu, X.Q. Zhu
Veterinary Parasitology 112(2003)101-108,-0001,():
-1年11月30日
The first internal transcribed spacer (ITS1) of nuclear ribosomal DNA from Culicoides arakawae was amplified by PCR, cloned and sequenced. The wDNAsis software was used to analyze the ITS1 sequences of C. arakawae and other nine species of Culicoides, which were obtained from GenBank and EMBL databases. For all species, the lengths of the ITS1 were 316-469 bp, and the G+C contents were 26.79-34.53%. Based on the lengths of the ITS1 sequences, the 10 Culicoides species could be divided into two groups. The first group consisted of C. arakawae, C. albicans, C. cubitalis, C. pulicaris and C. punctatus, and the second group comprised C. impunctatus, C. nubeculosus, C. variipennis, C. grisescens and C. imicola. The lengths for the first group were 316-347 bp and the second group were 457-469 bp. C. arakawae belonged to the first group by its ITS1 sequence length. Sequence analysis revealed that C. arakawae was genetically more similar to the first group than it was to the second group, consistent with results based on sequence length. The alignment of ITS1 (the alignment lengthwas 500 bp including the gaps) sequences showed that therewas a highly conserved region, which was between 288 and 388 bp, except for a few insertions and substitutions. These findings have important implications for the molecular identification of C. arakawae, for studying its molecular genetics and epidemiology, and for studying the molecular systematics of Culicoides.
Culicoides arakawae, ITS1, PCR, Alignment, Sequence analysis
-
49浏览
-
0点赞
-
0收藏
-
0分享
-
127下载
-
0评论
-
引用
朱兴全, J. M. de Gruijter, A. M. Polderman, X. Q. Zhu and R. B. Gasser*
Molecular and Cellular Probes (2002) 16, 185-190,-0001,():
-1年11月30日
Genetic markers in the mitochondrial genome have proven useful for population genetic studies because of their maternal inheritance and relatively high evolutionary rates. In this study, we exploited the high resolution capacity of PCR-coupled single-strand conformation polymorphism (SSCP) to screen for sequence variation in part of the cytochrome c oxidase subunit 1 gene (pcox1) among individuals of the parasitic nematode, Oesophagostomum bifurcum from human or Mona monkey hosts from Africa. SSCP analysis revealed distinct pro
Oesophagostomum bifurcum,, genetic variation,, mitochondrial cytochrome c oxidase subunit 1,, single-strand conformation polymorphism
-
45浏览
-
0点赞
-
0收藏
-
0分享
-
114下载
-
0评论
-
引用
朱兴全, X. Q. Zhu, , I. Beveridge, L. Berger, D. Barton and R. B. Gasser∗
Molecular and Cellular Probes (2002) 16, 159-165,-0001,():
-1年11月30日
This study examined genetic variability within Spirometra erinacei (Cestoda: Pseudophyllidea) from different host species and geographical origins in Australia using a polymerase chain reaction (PCR)-based mutation detection approach, followed by DNA sequencing. Part of the cytochrome c oxidase subunit 1 gene (pcox1) was amplified by PCR, scanned for sequence variation by singlestrand conformation polymorphism (SSCP), and representative samples from different host species were selected for DNA sequencing. While no variation in SSCP profiles was detected among S. erinacei samples from dog, fox, cat, tiger snake and python, they differed in profile from 5 specimens from the green tree frog (Litoria caerulea). This was supported by sequence data which demonstrated that pcox1 sequences of samples from the latter host species differed at 8 of 393 (2%) nucleotide positions from those from the non-amphibian host. Using a nucleotide difference in the pcox1 sequence, a PCR-linked restriction fragment length polymorphism (RFLP) could be employed to unequivocally delineate between samples from non-amphibian and amphibian hosts. These findings demonstrate the existence of at least two genotypes within S. erinacei, which may have important implications for studying the epidemiology, ecology and systematics of this cestode.
cytochrome c oxidase subunit 1 gene,, PCR,, restriction fragment length polymorphism (, RFLP), ,, single-strand conformation polymorphism (, SSCP), ,, Spirometra erinacei
-
40浏览
-
0点赞
-
0收藏
-
0分享
-
105下载
-
0评论
-
引用
朱兴全, X. Q. ZHU†, S. D'AMELIO, H.W. PALM, L. PAGGI, M. GEORGE-NASCIMENTO and R. B. GASSER*
Parasitology (2002), 124, 615-623.,-0001,():
-1年11月30日
The anisakid nematodes morphologically corresponding with Pseudoterranova decipiens sensu lato (s.l.) (Krabbe, 1878) from different seal or sea lion hosts and geographical origins, previously identified as Pseudoterranova krabbei, P. decipiens (s.s.), P. bulbosa, P. azarasi and P. cattani by multilocus enzyme electrophoresis, were characterized using a DNA approach. Also a population of P. decipiens (s.l.) from Chaenocephalus aceratus, the black
internal transcribed spacer,, polymerase chain reaction,, Pseudoterranova decipiens sensu lato,, ribosomal DNA,, single-strand conformation polymorphism,, species identi
-
70浏览
-
0点赞
-
0收藏
-
0分享
-
143下载
-
0评论
-
引用