洪枫
个性化签名
- 姓名:洪枫
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学术头衔:
教育部“新世纪优秀人才支持计划”入选者, 博士生导师
- 职称:-
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学科领域:
生物化学工程
- 研究兴趣:
洪枫,男,博导,中瑞工业生物技术国际合作研究室负责人,微生物工程与工业生物技术研究组长。生物工程学科带头人,生物化工硕士点负责人及学术带头人。教育部“新世纪优秀人才”,江苏省双创人才,上海高校“优秀青年教师基金”入选者,东华大学优秀青年教师。曾分别在瑞典隆德大学(Lund University)应用微生物系和瑞典Karlstad University生化系作博士后和高级客座研究员(1999-2003)。03年8月回国到校工作,主要从事生物工程的研究与教学工作。
目前兼任国际电化学能源科学院(IAOEES)委员,上海生物工程学会理事,上海化学化工学会生物技术与工程专业委员会委员,科技部国际科技合作重点项目计划评价专家,广东省生物产业协会顾问,《Biotechnology Frontier》杂志和《生物过程》杂志编委 ,21份国际杂志审稿人。 研究方向:生物催化与工业生物技术,生物医用材料,细菌纤维素的低成本高效制备及其应用,从事纺织工业生物技术、木质纤维素资源生物转化制备工业用酶和生物材料、环境生物技术等领域的研究。 曾先后承担完成了包括国家科技支撑计划课题、863计划新材料技术领域专题、国家自然科学基金、教育部和上海市科委等省部级课题十多项,目前正在主持1项瑞典-亚洲国际合作基金和3项上海市项目等。近年来在包括Chem Soc Rev(IF24.892)、Biotech Biofuels(IF6.09)、Biores Technol(IF4.98)、J Mater Chem(IF5.968)、Biosens Bioelectron(IF5.602)、Carbohydr Polym(IF3.628)、Biotechnol Bioeng(IF3.946)、Biotechnol Prog(IF2.34)、J Chem Technol Biotechnol(IF2.168)等国内外知名期刊上发表论文100余篇。以第一发明人申请PCT国际专利和国家发明专利68余项(29项已授权)。 近年重点开发细菌纤维素基新型医用敷料和人工血管等医用产品。“细菌纤维素(BC)高效生产与制品开发”获“纺织之光”2014年度科技奖一等奖(中国纺织工业联合会);“新型细菌纤维生物医用材料研发”项目荣获教育部颁发的“第十届中国海峡项目成果交易会高校优秀参展项目”证书。
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洪枫, Xiang Guo(郭香), Adnan Cavka, Leif J Jönsson and Feng Hong(洪枫)*
Microbial Cell Factories, 2013, 12: 93.,-0001,():
-1年11月30日
Background: Bacterial cellulose (BC) is a nanostructured material with unique properties and wide applicability. In order to decrease the production cost of bacterial cellulose, lignocellulose-based media have considerable potential as alternative cost-effective feedstocks. However, pretreatment and enzymatic hydrolysis of lignocellulose to sugars also generate fermentation inhibitors. Detoxification of lignocellulosic hydrolysates is needed to achieve efficient production of BC. In this investigation, different methods for detoxification of spruce hydrolysate prior to production of BC were compared with respect to effects on potential inhibitors and fermentable sugars, sugar consumption, BC yield, and cell viability. The objectives were to identify efficient detoxification methods and to achieve a better understanding of the role played by different inhibitors in lignocellulosic hydrolysates. Results: In a first series of experiments, the detoxification methods investigated included treatments with activated charcoal, alkali [sodium hydroxide, calcium hydroxide (overliming), and ammonium hydroxide], anion and cation ion-exchange resins, and reducing agents (sodium sulfite and sodium dithionite). A second series of detoxification experiments included enzymatic treatments (laccase and peroxidase). The potential inhibitors studied included aliphatic acids, furan aldehydes, and phenolic compounds. The best effects in the first series of detoxification experiments were achieved with activated charcoal and anion exchanger. After detoxification with activated charcoal the BC yield was 8.2 g/L, while it was 7.5 g/L in a reference medium without inhibitors. Treatments with anion exchanger at pH 10 and pH 5.5 gave a BC yield of 7.9 g/L and 6.3 g/L, respectively. The first series of experiments suggested that there was a relationship between the BC yield and phenolic inhibitors. Therefore, the second series of detoxification experiments focused on treatments with phenol-oxidizing enzymes. The BC yield in the laccase-detoxified hydrolysate reached 5.0-5.5 g/L after 14 days cultivation, which demonstrated the important inhibitory role played by phenolic compounds. Conclusions: The investigation shows that detoxification methods that efficiently remove phenolics benefit bacterial growth and BC production. Negative effects of salts could not be excluded and the osmotolerance of Gluconacetobacter xylinus needs to be further investigated in the future. Combinations of detoxification methods that efficiently decrease the concentration of inhibitors remain as an interesting option. ___________________________________________________ http://www.microbialcellfactories.com/content/12/1/93 http://www.microbialcellfactories.com/content/pdf/1475-2859-12-93.pdf
Bacterial cellulose,, Gluconacetobacter xylinus,, Norway spruce hydrolysate,, Detoxification methods
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【期刊论文】Production of bacterial cellulose and enzyme from waste fiber sludge
洪枫, Adnan Cavka, Xiang Guo(郭香), Shui-Jia Tang(唐水佳), Sandra Winestrand, Leif J. Jönsson, Feng Hong(洪枫)*
Biotechnology for Biofuels, 2013; 6: 25.,-0001,():
-1年11月30日
BACKGROUND: Bacterial cellulose (BC) is a highly crystalline and mechanically stable nanopolymer, which has excellent potential as a material in many novel applications, especially if it can be produced in large amounts from an inexpensive feedstock. Waste fiber sludge, a residue with little or no value, originates from pulp mills and lignocellulosic biorefineries. A high cellulose and low lignin content contributes to making the fiber sludge suitable for bioconversion, even without a thermochemical pretreatment step. In this study, the possibility to combine production of BC and hydrolytic enzymes from fiber sludge was investigated. The BC was characterized using field-emission scanning electron microscopy and X-ray diffraction analysis, and its mechanical properties were investigated. RESULTS: Bacterial cellulose and enzymes were produced through sequential fermentations with the bacterium Gluconacetobacter xylinus and the filamentous fungus Trichoderma reesei. Fiber sludges from sulfate (SAFS) and sulfite (SIFS) processes were hydrolyzed enzymatically without prior thermochemical pretreatment and the resulting hydrolysates were used for BC production. The highest volumetric yields of BC from SAFS and SIFS were 11 and 10 g/L (DW), respectively. The BC yield on initial sugar in hydrolysate-based medium reached 0.3 g/g after seven days of cultivation. The tensile strength of wet BC from hydrolysate medium was about 0.04 MPa compared to about 0.03 MPa for BC from a glucose-based reference medium, while the crystallinity was slightly lower for BC from hydrolysate cultures. The spent hydrolysates were used for production of cellulase with T. reesei. The cellulase activity (CMCase activity) in spent SAFS and SIFS hydrolysates reached 5.2 U/mL (87 nkat/mL), which was similar to the activity level obtained in a reference medium containing equal amounts of reducing sugar. CONCLUSIONS: It was shown that waste fiber sludge is a suitable raw material for production of bacterial cellulose and enzymes through sequential fermentation. The concept studied offers efficient utilization of the various components in fiber sludge hydrolysates and affords a possibility to combine production of two high value-added products using residual streams from pulp mills and biorefineries. Cellulase produced in this manner could tentatively be used to hydrolyze fresh fiber sludge to obtain medium suitable for production of BC in the same biorefinery. http://www.biotechnologyforbiofuels.com/content/6/1/25 http://www.biotechnologyforbiofuels.com/content/pdf/1754-6834-6-25.pdf
Bacterial cellulose,, Gluconacetobacter xylinus,, Enzyme production,, Cellulase,, Trichoderma reesei,, Fiber sludge
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【期刊论文】Biotransformation of wheat straw to bacterial cellulose and its mechanism
洪枫, Lin Chen(陈琳), Feng Hong(洪枫)*, Xue-xia Yang(杨雪霞), Shi-fen Han(韩士芬)
Bioresource Technology, 2013, 135, 464-468,-0001,():
-1年11月30日
An ionic liquid [AMIM]Cl was used to pretreat wheat straw with an aim to remarkably improve enzymatic hydrolysis rate and yield of fermentable sugars. Some influence factors including dosage of straw, particle size of straw meal as well as pretreatment time and temperature were investigated. After optimization,the hydrolytic efficiency of regenerated straw increased obviously as compared to untreated materials, and the sugar yield of straw was 71.2% after pretreatment in [AMIM]Cl at 110 oC for 1.5 h with a 3 w/w% straw dosage, 3.6 times higher than that of untreated straw (19.6%). The reason behind the acceleration of enzymatic hydrolysis was discussed by the analysis of SEM, XRD and FTIR. The yield of bacterial cellulose obtained in straw hydrolysates was higher than that in glucose-based media. This may be due to the presence of other complex components in the hydrolysate that would enhance the formation of bacterial cellulose.
Bacterial cellulose,, Biorefinery,, Wheat straw,, Ionic liquid pretreatment,, 1-Allyl-3-methylimidazolium chloride (, [AMIM]Cl),
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洪枫, Xiao Hua Wang(王小花), Da Nian Lu(陆大年), Leif J Jönsson, and Feng Hong(洪枫)*
Engineering in Life Sciences, 2008, 8(3): 268–276.,-0001,():
-1年11月30日
Optimization of cultivation conditions for lipase production and the preparation of a specific lipase catalyzing the hydrolysis of polyethylene terephthalate (PET) by Aspergillus oryzae CCUG 33812 as well as modification of PET fabrics by the enzyme were investigated. The lipase activity produced by the fungus after addition of olive oil was not efficient in changing the properties of PET samples. In order to obtain lipase activity directed towards hydrolysis of PET, two derivatives of terephthalic acid (TPA), namely diethyl p-phthalate (DP) and bis(2-hydroxyethyl) terephthalate (BHT), and PET short fibers were used as inducers. The results showed that BHT was the best inducer. The BHT-induced extracellular lipase could catalyze hydrolysis of the PET model substrate diethyl p-phthalate. The formation of new carboxyl groups is consistent with the increase in K/S values of dyed PET fabrics after the enzymatic treatment. Additionally, treatment with the BHT-induced lipase resulted in increased moisture regain and weight loss of PET fiber/fabric, while the water contact angle and the static half decay time decreased slightly. This indicates that hydrophilicity and anti-static ability were improved after the treatment with the BHT-induced lipase. Compounds that were solubilized by the enzymatic treatment were analyzed by spectrophotometry and high performance liquid chromatography with UV detection. Both analyses indicated that hydrolysis of PET occurred and that products were formed due to the catalytic action of the BHT-induced enzyme, however, no significant changes in UV absorbance and no new peaks were found in HPLC-UVD analyses of reaction mixtures with olive oil-induced lipase or PET-induced lipase. This investigation has resulted in detailed procedures for production of PET-hydrolyzing lipase by A. oryzae and is the first report regarding the successful use of BHT as an inducer for the enzyme production.
Polyethylene terephthalate, PET, Enzymatic modification, Aspergillus oryzae, Lipase, bis(, 2-hydroxyethyl), terephthalate
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洪枫, Cuixia Zhu(朱翠侠), Feng Hong(洪枫)*
Applied Biochemistry and Biotechnology, 2010, 160(2): 655-664.,-0001,():
-1年11月30日
In order to improve yields and to reduce the cost of oxalate decarboxylase (OxDC, EC 4.1.1.2), the induction of OxDC in the white-rot fungus Trametes versicolor was studied in this work. OxDC was induced by addition of inorganic acids including hydrochloric acid, sulfuric acid and phosphoric acid to culture media. The results showed that all the acids could enhance OxDC expression. The activity of the acid-induced OxDC rose continuously. All of the OxDC volumetric activities induced by the inorganic acids were higher than 20.0 U/L, and were two times higher than that obtained with oxalic acid. OxDC productivity was around 4.0 U•L-1•d-1. The highest specific activity against total protein was 3.2 U/mg protein at day 8 after induction of sulfuric acid and the specific activity against mycelial dry weight was 10.6 U/g at day 9 after induction of hydrochloric acid. The growth of mycelia was inhibited slightly when the pH values in culture media was around 2.5-3.0, while the growth was inhibited heavily when the pH was lower than 2.5.
Oxalate decarboxylase, Induction, Trametes versicolor, Low pH environment, Inorganic acid
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洪枫, Kun Zhong(钟坤), Zhi-Yu Shao(邵志宇), Feng Hong(洪枫)*
Biotechnology Progress, 2008, 24(3), 583-587.,-0001,():
-1年11月30日
Catechins are a group of polyphenolic compounds that are antioxidants having beneficial biological activities. There are four main catechins in green tea, and each has its own biological features. In order to fully exploit prominent biological activities of specific catechins and to develop new medicine from catechins, it is necessary to obtain pure catechin preparations by isolation from natural sources, by chemical synthesis, or by biotransformation reactions with high yield and specificity. In this study epigallocatechin gallate (EGCG) can be hydrolyzed to epigallocatechin (EGC) by a hydrolase from Aspergillus oryzae after induction by addition of EGCG to the cultures. However, cultures without EGCG induction did not show any EGCG hydrolysis activity. The yield of EGC could reach at least 70%. Thin layer chromatography and high performance liquid chromatography were applied to separate and quantify EGCG and EGC.
Catechin,, biotransformation,, epigallocatechin,, epigallocatechin gallate,, Aspergillus oryzae,, induction
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洪枫, 曹茂新, 洪枫*, 朱利民
中国生物工程杂志,2005,25(增刊):170-175,-0001,():
-1年11月30日
草酸脱羧酶是一种含锰的酶,在白腐菌中广泛存在,少数低等真菌和细菌中也能产生。目前,至少十多种草酸脱羧酶得到了分离和纯化。该酶是一种氨基酸残基在379个左右的单体酶,一般都为酸性糖蛋白,含有2个锰离子,形成2个活性区域;表面一些氨基酸被不同程度地糖基化。晶体结构和其它一些波谱学研究解释了其空间结构和可能的电子传递机制。运用PCR技术和cDNA文库技术,越来越多的草酸脱羧酶基因被克隆。已研究的该酶基因中都含有17个左右的内含子,这些内含子在活性域位置上有比较高的保守性。一些特殊氨基酸序列的存在决定了该酶的表达形式为诱导型,菌株的基因调控序列中含有一段受草酸化合物作用的序列。该酶在一些酵母和植物等异源表达系统中有成功表达的报道。该酶的应用主要集中在以下几方面:造纸废水中的草酸盐降解;食品中的草酸降解;草酸生物检测(如临床诊断)等。
草酸脱羧酶, 特性, 草酸降解, 应用
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洪枫, Kun Zhong, Sheng-Yin Zhao, Leif J. Jönsson, Feng Hong*
Biocatalysis and Biotransformation. 2008, 26(4), 306-312,-0001,():
-1年11月30日
Catechins are antioxidants known to exhibit beneficial biological activities. Five main catechins are found in green tea and each catechin has its own biological features. In order to fully exploit prominent biological activities of specific catechins and to develop new pharmaceuticals based on catechins, it is necessary to obtain pure catechin preparations by isolation from natural sources, or by chemical synthesis, or by biotransformation reactions with high yield and specificity. This study shows that epigallocatechin gallate (EGCG) can be hydrolysed to epigallocatechin (EGC) by a hydrolase produced by Aspergillus niger after induction by addition of EGCG to the fungal cultures. Cultures without EGCG induction and cultures, to which olive oil had been added as an inducer, did not show any EGCG hydrolysis activity. The yield of EGC could reach at least 56%. Methods based on thin-layer chromatography and high performance liquid chromatography, were used for separation and quantification of EGCG and EGC. Enzymatic conversion is an environmentally friendly and efficient approach to produce non-ester catechins.
Catechin,, biotransformation,, epigallocatechin,, epigallocatechin gallate,, Aspergillus niger,, induction
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洪枫, 谭玉静, 洪枫*, 邵志宇
中国生物工程杂,2007,27(4):126-131,-0001,():
-1年11月30日
细菌纤维素是一种天然的生物高聚物,具有生物活性、生物可降解性、生物适应性,具有独特的物理、化学和机械性能,例如高的结晶度、高的持水性、超细纳米纤维网络、高抗张强度和弹性模量等,因而成为近年来国际上新型生物医学材料的研究热点。概括细菌纤维素的性质、研究历史以及在生物医学材料上的应用,重点阐述细菌纤维素在组织工程支架、人工血管、人工皮肤和治疗皮肤损伤方面的应用以及当前研究现状。
细菌纤维素,, 组织工程支架,, 人工血管,, 人工皮肤
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洪枫, 谢健健, 洪枫*
,-0001,():
-1年11月30日
细菌纤维素是一种新型微生物合成材料,在食品、造纸、纺织、生物医药、声学器材振动膜和功能复合材料等方面均有很好的应用前景。细菌纤维素发酵培养基(尤其碳源)的成本是现今制约细菌纤维素推广应用的主要因素之一。甘露醇、果糖和葡萄糖等合成培养基所用碳源因其价格较高仅适用于实验室研究和小型发酵生产,规模化生产细菌纤维素的潜在原料应是一些量大价低的天然原料,包括水果类原料、糖质原料、低值淀粉类原料和废弃纤维素类原料等。木质纤维素原料是最具发展潜力的细菌纤维素碳源,也是细菌纤维素产业的根本出路,但目前存在一些技术瓶颈,制约了其开发利用,是一远期战略目标。文章简要介绍了细菌纤维素的基本情况,系统阐述了国内外发酵生产细菌纤维素原料的研究进展,展望了今后的发展趋势。
细菌纤维素, 原料, 碳源, 发酵培养基
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