张海霞
糖尿病研究中新分析方法的建立和样品预处理新方法的研究
个性化签名
- 姓名:张海霞
- 目前身份:
- 担任导师情况:
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学术头衔:
博士生导师, 教育部“新世纪优秀人才支持计划”入选者
- 职称:-
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学科领域:
分析化学
- 研究兴趣:糖尿病研究中新分析方法的建立和样品预处理新方法的研究
张海霞,女,教授,博士生导师。出生于1970年10月。1996年获得兰州大学分析化学硕士学位留校任教,2000年获得分析化学博士学位,2001年3月-2002年6月在比利时自由大学生物医学实验室作博士后研究,2007年4月起到韩国朝鲜大学药学院交流访问1年。
曾经或正在承担生命学院本科生的基础课《分析化学》、化学院本科生的《仪器分析》和化学院以及其他相关院系本科生的《检测化学》以及化学院硕士生专业课《现代液相色谱》、《基础分离科学》和博士生的基础课《分离化学》的课堂讲授工作。同时还承担了本科生大学化学实验和综合实验工作;指导多名本科生毕业论文;2003年开始独立招收硕士研究生,并和他人合作指导博士生。2004年6月本人荣获第三届“兰州大学教学新秀奖”一等奖;荣获兰州大学2005年度“三育人”先进个人称号。
自2003年以来,先后主持了国家基金1项,留学回国人员科研启动基金1项,霍英东优选课题1项,金川公司招标难题1项,金川公司预研基金多项,兰州大学骨干教师资助计划1项。荣获第12届甘肃省青年教师成才奖,发表学术论文50余篇,科研方向集中于糖尿病研究中新分析方法的建立和样品预处理新方法的研究。
目前担任兰州大学-金川公司联合实验室副主任,生命分析化学与原子分子工程研究所副所长。
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225
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成果数
5
张海霞, Cailing Yang, Linyuan Guo, Xiaoyan Liu, Haixia Zhang*, Mancang Liu
Journal of Chromatography A, 1164(2007)56-64,-0001,():
-1年11月30日
Tetrandrine (TET) and fangchinoline (FAN) are basic and highly hydrophobic drugs with log P>5.7. In this work, a simple, inexpensive and efficient liquid-phase microextraction (LPME) technology combined with high-performance liquid chromatography (HPLC) was developed for the simultaneous analysis of tetrandrine and fangchinoline in plasma samples. Tetrahydropalmatine was used as internal standard. Several parameters influencing the efficiency of LPME were investigated and optimized including organic solvent, stirring rate, extraction time, salt concentration, organic modifier and pH. Under the optimal conditions, extraction recoveries from plasma samples were 46% for tetrandrine and 50% for fangchinoline, corresponding to the drugs enriched by a factor of 23 and 25 by LPME, respectively. Excellent sample clean-up was observed and good linearities with correlation coefficients (r) of 0.9979 (FAN) and 0.9995 (TET) were obtained in the range of 15-1000 ngmL−1. The limits of detection (LOD, S/N=3) were 3.0ngmL−1 for FAN and 2.0ngmL−1 for TET.
Hollow fiber liquid-phase microextraction, High-performance liquid chromatography, Tetrandrine and fangchinoline, Plasma sample
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张海霞, Xiaoyan Liu, Yongsheng Ji, Yonghui Zhang, Haixia Zhang*, Mancang Liu
Journal of Chromatography A, 1165(2007)10-17,-0001,():
-1年11月30日
A simple and environmentally friendly method for determination of seven phenols using solid-phase microextraction (SPME) coupled to highperformance liquid chromatography (HPLC) has been developed. Several materials were used as stationary phase of SPME fibers and an oxidized multiwalled carbon nanotubes material was found to be effective in carrying out simultaneous extraction of phenols in aqueous samples. Compared with the widely used commercially available SPME fibers, this proposed fiber had much lower cost, longer lifetime (over 150 times), shorter analysis time (30 min of extraction and 3 min of desorption time) and comparable or superior extraction efficiency for the investigated analytes. The extraction and desorption conditions were evaluated and the calibration curves of seven phenols were linear (R2≥0.9908) in the range from 10.2 to 1585 ngmL−1. The limits of detection at a signal-to-noise (S/N) ratio of 3 were 0.25-3.67 ngmL−1, and the limits of quantification calculated at S/N=10 were 0.83-12.25 ngmL−1 for these compounds. The possibility of applying the proposed method to environmental water samples analysis was validated.
Phenols, Oxidized multiwalled carbon nanotubes, Solid-phase microextraction-high performance liquid chromatography
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张海霞, Huayu Huang, Cailing Yang, Haixia Zhang*, Mancang Liu
Microporous and Mesoporous Materials 111(2008)254-259,-0001,():
-1年11月30日
Octyl (C8) or octadecyl (C18)-modified mesoporous SBA-15 silica molecular sieves have been prepared by adding SBA-15 silica molecular sieves to octyltrimethoxysilane or octadecyltrimethoxysilane in toluene at 100 C, and characterized by Fourier transform infrared (FTIR) spectroscopy, powder X-ray diffraction (XRD), nitrogen adsorption-desorption measurements, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). FTIR spectra shows the presence of methylene (-CH2-) and methyl (-CH3) bands on the modified SBA-15. Powder XRD data indicate the structure of modified SBA-15 with octyl or octadecyl groups still remains twodimensional hexagonal mesostructrure. Brunauer-Emmett-Teller (BET) surface area analysis presents that surface area of octyl-and octadeyl-SBA-15 changed from 647 to 449 and 321 m2g-1, respectively, and SEM images show the decreased size of modified SBA-15 particles. TEM images of modified materials with alkyl groups show the structures remain the same as the parent SBA-15 silica. We also have studied the adsorption capacity of the materials to phthalate esters (dimethyl and diethyl phthalate) by dynamic adsorption experiments on high performance liquid chromatography (HPLC). It is found that the modified materials can increase the adsorption of phthalate esters compared to SBA-15 particles, and the adsorption capacity increased with the increased length of alkyl chain on SBA-15. The maximum dynamic adsorption capacity for diethyl phthalate was 3.9 (C8-SBA-15) or 4.3 (C18-SBA-15) times higher than that of SBA-15 particles, respectively. The results indicate that alkyl SBA-15 particles could be used for enrichment of phthalate esters in water samples before the further analysis.
Mesoporous silica molecular sieves, Alkyl group, Phthalates, Adsorption, High performance liquid chromatography
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张海霞, Xiaoman Jiang, Wei Tian, Chuande Zhao, Haixia Zhang*, Mancang Liu
Talanta 72(2007)119-125,-0001,():
-1年11月30日
A novel and simple imprinted amino-functionalized silica gel material was synthesized by combining a surface molecular imprinting technique with a sol-gel process on the supporter of activated silica gel for solid-phase extraction-high performance liquid chromatography (SPE-HPLC) determination of bisphenol A (BPA). Non-imprinted silica sorbent was synthesized without the addition of BPA using the same procedure as that of BPA-imprinted silica sorbent. The BPA-imprinted silica sorbent and non-imprinted silica sorbent were characterized by FT-IR and the static adsorption experiments. The prepared BPA-imprinted silica sorbent showed high adsorption capacity, significant selectivity and good site accessibility for BPA. The maximum static adsorption capacity of the BPA-imprinted and non-imprinted silica sorbent for BPA was 68.9 and 34.0mg g−1, respectively. The relatively selective factor value of this BPA-imprinted silica sorbent was 4.5. Furthermore, the difference of the retention characteristics of BPA on the C8 SPE column and BPA-imprinted silica SPE (MIP-SPE) was compared. The MIP-SPE-HPLC method showed higher selectivity to BPA than the traditional SPE-HPLC method. At last, the BPA-imprinted polymers were used as the sorbent in solid-phase extraction to determine BPA in water samples with satisfactory recovery higher than 99% (R.S.D. 3.7%).
Molecularly imprinted polymers, Bisphenol A, Solid-phase extraction, High performance liquid chromatography
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张海霞, C.Y. Zhao a, H.X. Zhang a, *, X.Y. Zhang a, M.C. Liu a, Z.D. Hu a, B.T. Fan b
Toxicology 217(2006)105-119,-0001,():
-1年11月30日
As a new method, support vector machine (SVM) were applied for prediction of toxicity of different data sets compared with other two common methods, multiple linear regression (MLR) and RBFNN. Quantitative structure-activity relationships (QSAR) models based on calculated molecular descriptors have been clearly established. Among them, SVM model gave the highest q2 and correlation coefficient R. It indicates that the SVM performed better generalization ability than the MLR and RBFNN methods, especially in the test set and the whole data set. This eventually leads to better generalization than neural networks, which implement the empirical risk minimization principle and may not converge to global solutions. We would expect SVM method as a powerful tool for the prediction of molecular properties.
Quantitative structure-activity relationship (, QSAR), , Toxicity, Multiple linear regression (, MLR), , Radical basis function neural network (, RBF), , Support vector machine (, SVM),
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