李毅
一、对我国水稻重要病原,水稻矮缩病毒(RDV)进行了系统深入的研究; 二、拟南芥转录调控因子中WRKY家族蛋白结构和功能研究;
个性化签名
- 姓名:李毅
- 目前身份:
- 担任导师情况:
- 学位:
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学术头衔:
博士生导师, 教育部“新世纪优秀人才支持计划”入选者
- 职称:-
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学科领域:
生物化学
- 研究兴趣:一、对我国水稻重要病原,水稻矮缩病毒(RDV)进行了系统深入的研究; 二、拟南芥转录调控因子中WRKY家族蛋白结构和功能研究;
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【期刊论文】Rice Dwarf Phytoreovirus Segment S11 Encodes a Nucleic Acid Binding Protein
李毅, Hong Xu, *, Yi Li, †, , Zhijuan Mao, Yiyun Li, Zhijin Wu, Lin Qu, Chengcai An, Xiaotian Ming, Joachim Schiemann, ‡, Rudol Casper, ‡ and Zhangliang Chen*
VIROLOGY 240, 267-272(1998),-0001,():
-1年11月30日
The function of rice dwarf virus segment 11 and the corresponding segments of other phytoreoviruses is not yet determined. The amino acid sequence of Pns11, encoded by segment 11, contains a putative zinc finger and five flanking basic regions at the C-terminus. The full-length Pns11 protein and three truncated derivatives, which lack the N-terminus, the zinc-finger, or the C-terminal five basic regions were expressed in Escherichia coli and their nucleic acid binding properties were studied. Pns11 interacts with single- and double-stranded forms of DNA and RNA in a sequence-nonspecific manner. The truncated derivative which contains both the zinc-finger and the C-terminal basic regions has the same binding properties as the full-length Pns11. However, removal of either of these domains prevents binding activity. The binding activity of Pns11 was drastically reduced when the blots were treated with a high concentration of EDTA. Moreover, Pns11 extracted from infected rice also binds to single-stranded RNA. These data suggest that RDV Pns11 binding activity is structuredependent and it may play an important role in virus replication and/or genome assortment.
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【期刊论文】Safety assessment for genetically modified sweet pepper and tomato
李毅, Zhang-Liang Chen a, *, Hongya Gua, Yi Li a, Yilan Su b, Ping Wub, Zhicheng Jiang b, Xiaotian Ming a, Jinhua Tian a, Naisui Pan a, Li-Jia Qu a
Toxicology 188(2003)297-307,-0001,():
-1年11月30日
The coat protein (CP) gene of cucumber mosaic virus (CMV) was cloned from a Chinese CMV isolate, the CaMV promoter and NOS terminator added and the gene construct was transformed into both sweet pepper and tomato plants to confer resistance to CMV. Safety assessments of these genetically modified (GM) plants were conducted. It was found that these two GM products showed no genotoxicity either in vitro or in vivo by the micronucleus test, sperm aberration test and Ames test. Animal feeding studies showed no significant differences in growth, body weight gain, food consumption, hematology, blood biochemical indices, organ weights and histopathology between rats or mice of either sex fed with either GM sweet pepper or tomato diets compared with those with non-GM diets. These results demonstrate that the CMV-resistant sweet pepper and tomato are comparable to the non-GM counterparts in terms of food safety.
GM plants, Safety assessment, CMV-cp, Feeding test
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李毅, Ming-Rui Duana, c, Hui Renb, Peng Maoa, Chun-Hong Weia, Yu-He Liangb, *, Yi Lia, Xiao-Dong Sub
Biochimica et Biophysica Acta 1750(2005)14-16,-0001,():
-1年11月30日
The C-terminal WRKY domain of Arabidopsis thaliana WRKY1 protein, a transcription factor, was cloned and expressed. The expressed protein was then purified and crystallized. The preliminary X-ray analysis was undertaken. The crystal diffracted to 2.50 A resolution inhouse and belongs to space group P21 with unit-cell parameters a=64.10 A°b, b=34.88 A°c, c=114.72 A°β, b=90.49°.
WRKY protein, Trans, c, r, i, p, t, ion factor, Crystallization, X-ray diffraction
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