林建强
工业微生物基因工程和代谢工程育种;发酵工程;生物反应过程数学建模、在线监测与自动控制;天然药物及其生物转化
个性化签名
- 姓名:林建强
- 目前身份:
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学术头衔:
博士生导师
- 职称:-
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学科领域:
发酵工程
- 研究兴趣:工业微生物基因工程和代谢工程育种;发酵工程;生物反应过程数学建模、在线监测与自动控制;天然药物及其生物转化
林建强,现为山东大学教授,博士生导师。于1988、1991和1998年在山东大学分别获得微生物工程学学士、微生物学硕士和微生物学博士学位。其中,1996至1998获得中日联合培养博士生项目资助在大阪大学从事博士论文研究;2000-2001在韩国仁荷大学做博士后研究;2002、2003在韩国做短期访问研究。 1991年硕士毕业留山东大学任教至今,1999年任副教授,2002年任教授,2003年为博士生导师。主要研究兴趣包括工业微生物基因工程和代谢工程育种;发酵工程;生物反应过程数学建模、在线监测与自动控制;天然药物及其生物转化。目前主要研究内容包括微生物冶金、微生物采油、生物质资源的生物转化、发酵过程在线监测与控制、天然药物生物转化。在工业微生物育种方面,构建了在厌氧条件下以石油烃为碳源生产生物表面活性剂的驱油基因工程菌、具有毒性离子抗性的生物冶金基因工程菌、生产金属硫蛋白和生产降血栓活性蛋白的基因工程菌;在发酵工程方面,研究了微生物浸矿、纤维素酶发酵、乳酸发酵以及纤维素生物转化生产乳酸、乙醇等发酵工艺;在数学模型与在线监测与控制方面,研究了传统数学模型以及遗传算法和模糊数学模型,编写了过程模拟与过程控制的计算机软件,构建了菌体浓度等在线监测装置;在天然药物方面在药物分析、提取和生物转化方面进行了研究。
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585
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成果数
12
【期刊论文】Modeling of Typical Microbial Cell Growth in Batch Culture
林建强, Jianqiang Lin '*, Sang-Mok Lee , Ho-Joon Lee , and Yoon-Mo Koo
Biotechnol. Bioprocess Eng. 2000, 5: 382-385,-0001,():
-1年11月30日
A mathematical model was developed, based on the time dependent changes of the specific growth rate, for prediction of the typica.l'microbihl ceil growth in batch cultures. This model could predict both the lag growth phase and the stationary growth phase of batch cultures, and it was tested with the batch giowth of Trichoderma reesei and Lactoba-cillus delbrueckii.
microbial cell growth, mathematical model, specific growth rate, batch culture
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林建强, 宋茂勇, 李清心, 曲音波, 魏玉华
生物技术,2003,13(2)16~18,-0001,():
-1年11月30日
菌株G31是从胜利油田污水中分离到的1株假单胞菌(Paeudomonas sp),该菌株在石油液体培养基中够生长发西酵,产生有机酸和表面活性物质,可以应用于微生物三次采油。菌株G31可以降解和乳化石油、降低石油的粘度,其产物可以降低渍水的界面张力,增加石油的流动性。室内岩心试验结果表明利用菌株G31发酵液进行驱油可使原油采收率提高10%以上。
有机酸, 乳化, 界面张力, 微生物三次采油
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林建强, 王丹, , 张志华, 曲音波, 余世袁
生物加工过程,2005,3(1)32~36,-0001,():
-1年11月30日
粗糙脉孢茵(Neurospora crassa)具有直接转化植物纤维性物质生产乙醇的能力。研究了不同氧限制条件对粗糙脉孢茵发酵葡萄糖生产乙醇的影响,构建了该过程的数学模型,并利用数学模型进行了模拟和预测研究。结果表明,数学模型能够很好地预测氧限制条件下乙醇的发酵过程,即使微量氧对乙醇发酵也有较大的负面影响。
粗糙脉孢茵, 数学模型, 乙醇发酵, 氧限制
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林建强, 宋茂勇, 魏玉华, 李强
山东大学学报(理学版),2004,39(1)117~120,-0001,():
-1年11月30日
从油田废水中分离到一株芽孢杆茵(Bacillus sp),并对其降解原油的能力进行了研究。结果表明,其具有明显的降解原油的能力,并能够发酵产生有机酸和生物表面活性物质,可以用于微生物三次采油。实验还表明,茵株作用于高粘度原油的降粘效果要好于作用于低粘度原油的效果。
原油降解, 有机酸, 生物表面活性剂, 三次采油
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53浏览
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【期刊论文】Possible strategy for on-line monitoring and control of hybridoma cell culture
林建强, Jianqiang Lin a, ∗, Mutsumi Takagi b, Yinbo Qua, Toshiomi Yoshida b
Biochemical Engineering Journal 11(2002)205-209,-0001,():
-1年11月30日
The system and the method for on-line monitoring of the oxygen uptake rate (OUR) in bioreactors, based on the dynamic measurement of dissolved oxygen (DO), were developed and tested. Strategies for maintaining the energy metabolic rate while reducing the production of ammonia and lactic acid of hybridoma cells via the controlled addition of glutamine and glucose were demonstrated. The relation coupling the consumptions of oxygen and glutamine was used to calculate the glutamine feeding rate.
Animal cell culture, Bioreactions, Fed-batch culture, Hybridoma cultures, Oxygen uptake rate, On-line monitoring, Monoclonal antibody
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林建强, LIN, JIAN-QIANG , SANG-MOK LEE , AND YOON-MO KOO *
J. Microbiol. Biotechnol. (2005), 15(1),40-47,-0001,():
-1年11月30日
Modeling and simulation for simultaneous saccharification and fermentation (SSF) process in bioconversion of paper mill sludge to lactic acid was carried out. The SSF process combined the enzymatic hydrolysis of paper mill sludge into glucose and the fermentation of glucose into lactic acid in one reactor. A mathematical modeling for cellulose hydrolysis was developed, based on the proposed mechanism of cellulase adsorption deactivation. Another model for simple lactic acid fermentation was also made. A whole mathematical model for SSF was developed by combining the above two models for cellulose hydrolysis and lactic acid fermentation. The characteristics of the SSF process were investigated using the mathematical model.
Cellulase, cellulose, paper mill sludge, lactic acid, modeling
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林建强, Jian-qiang Lin , Sang-Mok Lee , and Yoon-Mo Koo *
Biotechnology and Bioprocess Engineering 2004, 9: 52-58,-0001,():
-1年11月30日
An unstructured mathematical model for lactic acid fermentation was developed. This model was able to predict the inhibition effects of lactic acid and glucose and was con-firmed to be valid with various initial concentrations of lactic acid and glucose. Simulation of en-ergy production was made using this mathematical model, and the relationship between the ki-netics of energy metabolism and lactic acid production was also analyzed.
modeling, lactic acid, glucose, inhibition, energy balance
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林建强, LIN, JIAN-QIANG , SANG-MOK LEE , AND YOON-MO KOO *
J. Microbiol. Biotechnol. (2004), 14(6),1163-1169,-0001,():
-1年11月30日
An unstructured mathematical model is presented for lactic acid fermentation based on the energy balance. The proposed model reflects the energy metabolic state and then predicts the cell growth, lactic acid production, and glucose consumption rates by relating the above rates with the energy metabolic rate. Fermentation experiments were conducted under various initial lactic acid concentrations of 0, 30, 50, 70, and 90g/l. Also, a genetic algorithm was used for further optimization of the model parameters and included the operations of coding, initialization, hybridization, mutation, decoding, fitness calculation, selection, and reproduction exerted on individuals (or chromosomes) in a population. The simulation results showed a good fit between the model prediction and the experimental data. The genetic algorithm proved to be useful for model parameter optimization, suggesting wider applications in the field of biological engineering.
Model, lactic acid, fermentation, genetic algorithm, optimization
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【期刊论文】Metabolic Flux Change in Hybridoma Cells under High Osmotic Pressure
林建强, JIANQIANG LIN, , MUTSUMI TAKAGI, * YINBO QU, PEIJI GAO, AND TOSHIOMI YOSHIDA
JOURNAL OF BIOSClENCE AND BIOENGINEERING, 87(2)255-257. 1999,-0001,():
-1年11月30日
The effect of high osmotic pressure on the flux changes of two energy metabolisms from glucose and gluta-mine by AFP-27 hybridoma cells producing monoclonal antibody (MAb) was investigated in batch cultures at various osmotic pressures in the range from 300 to 424mosmol/kg. The specific production rate of MAb (qMAb) increased monotonically with increasing osmotic pressure. The specific consumption rates of glucose 0.1) and glutamine (μLN) also increased with the osmotic pressure up to 410 mOsmoi/kg. However, there were marked changes in the metabolic fluxes when the osmotic pressure was raised further, resulting in a decrease in while μGLN continued to increase. These changes in the metabolic fluxes at osmotic pressures higher than 410 mOsmol/kg were associated with an increased yield of lactic acid from glucose (YL/G), which indicated the energy yield from glucose declined at osmolarities higher than 410 mOsmol/kg. On the other hand, because of a larger increment in the specific production rate of ammonia under high osmotic pressure, the yield of ammonia from glutamine (YA/cLN) increased monotonically with the osmotic pressure throughout the range 300 to 424mosmol/kg, signifying a rise in the energy yield from glutamine. Consequently, the higher specific ATP production rates from glucose and glutamine associated with the flux changes under high osmotic pressure could be one of the reasons for the increase in qMAb observed at high osmotic pressures.
hybridoma, osmotic pressure, metabolic flux, antibody production
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