齐义鹏
杆状病毒,医学病毒和人类基因组
个性化签名
- 姓名:齐义鹏
- 目前身份:
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学术头衔:
博士生导师
- 职称:-
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学科领域:
细胞生物学
- 研究兴趣:杆状病毒,医学病毒和人类基因组
齐义鹏教授,武汉大学生命科学学院教授,1961年毕业于武汉大学生物系微生物专业,1990年被国务院批准为博士生导师,1992年享受国务院特殊津贴。1982-1984年赴美国Ohio州立大学进修2年,1991-1992赴美国加州大学合作研究1年,曾任武汉大学学位委员会委员,职称评定委员会委员,病毒系主任,病毒研究所所长,生物技术系主任,国家微生物重点学科和国家病毒学211工程重点建设学科负责人。2001年被授予中国科协先进科技工作者。2002年被授予湖北省优秀科技工作者称号。
齐义鹏教授以杆状病毒,医学病毒和人类基因组为研究方向,获科研成果奖6项,包括国家自然科学二等奖(1991,9120904)。教育部科技进步一等奖(1991,06804),教育部自然科学一等奖(2003,2003-029-01),江苏省科技进步一等奖(1-10-03),湖北省自然科学二等奖(2002Z-040-2-016-008-R01)。发表论文258篇,其中SCI论文68篇,影响因子大于3的论文12篇。著作3部。
已毕业硕士研究生26人其中留学生1人,博士研究生29人,其中留学生2人,已出站博士后2人。有35人在国外深造,已回国的学生中,有3人晋升为教授和博士生导师,6人晋升为副教授。不少人已成长为优秀科技工作者,其中庞代文教授是武汉大学化学学院院长,博导,国家杰出青年基金获得者。博士生杜全胜在JV上发表的论文获2000年湖北省优秀论文特等奖。
在齐义鹏教授长期担任武汉大学微生物-病毒学科负责人期间,组织实施211工程病毒学第一期建设项目,圆满完成建设目标,在2000年的验收中被评为优秀,并顺利进入第二期211工程建设项目。武汉大学微生物重点学科,在2001年的评审中,以全国二级学科(微生物学)总分第一,一级学科(生物学)排名第十的优势被继续批准为全国重点学科。他除讲授本科生《基因生物学》外,还为研究生讲授必修课《基因工程原理》,《现代分子生物学导论》20多年。他出版的著作《基因工程原理和方法》和《基因及其操作原理》被国内一些高等学校作为研究生教材,获得较好评价。由于在研究生培养方面的突出成绩,齐义鹏教授获1993年湖北省高校研究生教学二等奖。
齐义鹏教授参加了广泛的国内外学术会议,多次在国内受到邀请作大会报告,3次在国际会议上作邀请报告,得到学术界的较好评价。
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3616
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成果阅读
1251
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成果数
20
齐义鹏, Zifei Pei‡§¶, Galit Reske‡¶, Qihong Huang**, Bruce D. Hammock, Yipeng Qi§, and Nor Chejanovsky‡ ‡‡
THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 277, No.50, Issue of December 13, pp. 48677-48684, 2002,-0001,():
-1年11月30日
Two antiapoptotic types of genes, iap and p35, were found in baculoviruses. P35 is a 35-kDa protein that can suppress apoptosis induced by virus infection or by diverse stimuli in vertebrates or invertebrates. iap homologues were identified in insects and mammals. Recently, we have identified sl-p49, a novel apoptosis suppressor gene and the first homologue of p35, in the genome of the Spodoptera littoralis nucleopolyhedrovirus. Here we show that sl-p49 encodes a 49-kDa protein, confirmed its primary structure that displays 48.8% identity to P35, and performed computer-assisted modeling of P49 based on the structure of P35. We demonstrated that P49 is able to inhibit insect and human effector caspases, which requires P49 cleavage at Asp94. Finally we identified domains important for P49's antiapoptotic function that include a reactive site loop (RSL) protruding from a B-barrel domain. RSL begins at an amphipathic a1 helix, traverses the a-sheet central region, exposing Asp94 at the apex, and rejoins the a-barrel. Our model predicted seven a-helical motifs, three of them unique to P49. a-Helical motifs a1, a2, and a4a were required for P49 function. The high structural homology between P49 and P35 suggests that these molecules bear a scaffold common to baculovirus "apoptotic suppressor" proteins. P49 may serve as a novel tool to analyze the contribution of different components of the caspase chain in the apoptotic response in organisms not related
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【期刊论文】Mammalian apoptosis-inducing protein, HAP, induces bacterial cell death
齐义鹏, Miao Gan, Yipeng Qi, Qingwen Wan, Ersheng Kuang, Qingzhen Liu & Xin Liu
Molecular Biology Reports 31: 159-164, 2004.,-0001,():
-1年11月30日
In attempting to produce the HAP, endoplasmic reticulum (ER) targeted apoptosis-inducing protein, as a GSTfusion protein we found that the expression of HAP, but not GST alone, induced bacterial cell death. The HAP protein inhibited the bacterial growth within 30min after inducting HAP expression. The transmission electron microscopic examination revealed that the morphology of the bacterial cells expressing hap was changed dramatically: unusually elongated phenotype compared with those of controls and finally leading to cell death. The lethality of HAP was relieved by the addition of vitamin E as a reducing agent and under anaerobic growth conditions. These results suggest that a trace amount of HAP induces bacterial cell death and the death is related with reactive oxygen species (ROS).
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【期刊论文】Isolation and characterization of a human apoptosisinducing gene with yeast two-hybrid system
齐义鹏, QI Bing QI Yipeng Masuo Yutsudo & LIU Qingzhen
SCIENCE IN CHINA (Series C) Vol. 43 No.3 June 2000,-0001,():
-1年11月30日
asy gene is a novel apoptosis-inducing gene, but its mechanism is unclear. To investigate the mechanism of asy inducing apoptosis, a novel gene encoding ASY interacting protein (asyip) is isolated from human lung cell line (WI-38) cDNA library with yeast two-hybrid system. The asyip gene is constitutively expressed as two mRNA transcripts with the size of 1.8 and 2.7kb in various human tissues at different levels. Sequence analysis of full-length cDNA reveals that the two alternative transcripts of asyip gene contain common 5' end and different 3' end, and share a common open reading frame encoding a polypeptide of 236 amino acids. Two protein kinase C phosphorylation sites and two casein kinase II phosphorylation sites are found in ASYIP amino acid sequence. Two highly hydrophobic regions encoding potentially two transmembrane domains are present. The ASYIP protein contains a C-terminal endoplasmic reticulum retrieval signal (Lys-Lys-Lys-Ala-Glu). Immunoprecipitation assay confirmed the interaction of ASY and ASYIP in mammalian cells. Compared with asy gene, overexpression of asyip gene can inhibit growth of tumor cell Saos2 and induce cell apoptosis with a low efficiency.
asy gene,, asyip gene,, apoptosis,, yeast two-hybrid system,, human lung cell cDNA library
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齐义鹏, PEI Zifei, QI Yipeng, LIU Yingle & XIAO Huazhong
Chinese Science Bulletin Vol. 47 No.8 April 2002,-0001,():
-1年11月30日
A novel cloned Spodoptera littoralis Nucleopolyhedrovirus (SlNPV) p49 gene is able to suppress apoptosis of insect cells Sf9 triggered by virus. The amino acid sequence of P49 expressed in baculovirus expression system is the same as predicted, indicating that the expression of P49 is correct. Metabolic labeling revealed that p49 was able to be expressed both in the early and late phases after the viral infection, and only in the late phase was the expression driven by polyhedra promoter, but the amount of expression was higher than that of wtSlNPV. In summary, the early gene of SlNPV p49 as well as p35 of AcMNPV is able to be expressed in the late phase, but its promoter is weaker compared with polyhedra promoter. In vitro, P49 can be cut by Bm caspase and human caspase-3, yielding 10 and 40 ku fragments. Purified P49 blocks the substrate cleavage by Bm caspase and human caspase-3, showing that P49 inhibits downstream caspases in the apoptotic pathway.
apoptosis,, p49 gene,, Caspase.,
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【期刊论文】asy and asyip: A new type of apoptosis-inducing gene
齐义鹏, QI Bing, QI Yipeng, LIU Qinzheng & QU Xiaoling
Chinese Science Bulletin Vol. 46 No.17 September 2001,-0001,():
-1年11月30日
Apoptosis, a wide physiological process in multicellular organisms, is critical for the organ development, the cell senescence, the tissue homeostasis and the resistance to infection of virus and bacteria. Apoptosis plays a key role in the regulation of the growth cell number such as the tissue construction, the elimination of abnormal or dangerous cells. Therefore, apoptosis is a stringent and effective cell quality controlling system, which reduces the number of harmful cells to the maximum, such as auto-immunity cell, cells infected by virus, tumor cells by cell suicide[1]. Apoptosis can be initiated by a wide variety of the extracellular and intracellular stimuli, including the developmental signals, the cellular stress and the disruption of cell cycle, transduced and amplified by the second messengers, and finally finished by the activating death effector protease. This process can be called apoptosis signal network. The defective in control of the apoptotic pathways may contribute to a variety of diseases including cancer, autoimmune and neurodegenerative conditions [2,3].
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【期刊论文】Pro-Apoptotic ASY/Nogo-B Protein Associates With ASYIP
齐义鹏, BING QI, , YIPENG QI, AKIHIRO WATARI, NAOHISA YOSHIOKA, HIROKAZU INOUE, YUZURU MINEMOTO, KATSUMI YAMASHITA, TOSHIYUKI SASAGAWA, AND MASUO YUTSUDO *
JOURNAL OF CELLULAR PHYSIOLOGY 196: 312-318 (2003),-0001,():
-1年11月30日
We have previously shown that ectopic expression of the ASY/Nogo-B gene induced apoptosis in various cancer cell lines. Nogo-A, a splice variant of the ASY, has been reported to have an inhibitory effect on neuronal regeneration in the central nervous system. To investigate the mechanism of ASY-induced apoptosis or inhibition of neuronal regeneration, we cloned a cDNA for the ASY-interacting protein from the human cDNA library using the yeast two-hybrid method, and obtained a cDNA we designated as ASYIP. The ASYIP protein contains two hydrophobic regions and a double lysine endoplasmic reticulum (ER) retrieval motif at its C-terminus, which was shown to be identical to RTN3, a reticulon family protein of unknown function. We showed that ASY and ASYIP proteins formed a complex also in human cells. Mutational analysis indicated that both of the hydrophobic regions of the ASYIP protein were required for the association. By immunofluorescence analysis, the ASYIP protein was shown to be co-localized with ASY in the ER. Characterization of the ASYIP gene may be very useful in clarifying the mechanism of ASY-induced apoptosis or Nogo-involved inhibition of neuronal regeneration in the central nervous system. J. Cell. Physiol. 196: 312-318, 2003.
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齐义鹏, QINGZHEN LIU, , YIPENG QI & NOR CHEJANOVSKY, †
Virus Genes 26: 2, 143-149, 2003,-0001,():
-1年11月30日
Baculoviruses possess two types of genes that suppressed apoptosis, p35 and inhibitor of apoptosis (iap). In this study we report the isolation and identification of an inhibitor of apoptosis gene Sliap in the genome of the Spodoptera littoralis nucleopolyhedrvirus (SINPV). The Sliap sequence predicted a 15kDa polypeptide with only on BIR domain and a RING finger, both motifs characteristic of the IAP family of proteins, and a third specific acidic-rich motif. These characteristics, shared with the Spodoptera littura NPV IAP2/3, Epiphyas postvittana NPV IAP4, Lymantria dispar NPV IAP and Orgyia pseudotsugata NPV IAP4 (Orf 107) allowed us to classify them in a new homology group (IAP-4). Sliap was able to delay, but not to suppress,apoptosis induced by replication of a recombinant AcMNPV deficient in p35. In SINPV infected-SF9 cells Sliap was expressed earlier than sl-p49 suggesting that its role at the initiation of infection was to delay the apoptotic response of the host.
Anti-apoptotic genes,, iap,, insect cells,, SINPV
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齐义鹏, Ping Lan, Changyuan Dong, Yipeng Qi*, Gengfu Xiao and Feng Xue
,-0001,():
-1年11月30日
A mutant herpes simplex virus 1, mtHSV, was constructed by inserting the E. coli beta-galactosidase gene into the loci of icp34.5, the apoptosis-inhibiting gene of HSV. The mtHSV replicated in and lysed U251 (human glioma cells), EJ (human bladder cells), and S-180 (mice sarcoma cells), but not Wish (human amnion cells) cells. With its intact tk (thymidine kinase) gene, mtHSV exhibited susceptibility to acyclovir (ACV), which provided an approach to control viral replication. An in vivo test with mtHSV was conducted in immune-competent mice bearing sarcoma S-180 tumors, which were treated with a single intratumoral injection of mtHSV or PBS. Tumor dimensions then were measured at serial time points, and the tumor volumes were calculated. Sarcoma growth was significantly inhibited with prolonged time and reduced tumor volume. There was microscopic evidence of necrosis of tumors in treated mice, whereas no damage was found in other organs. Immunohistochemical staining revealed that virus replication was exclusively confined to the treated tumor cells. HSV-1 DNA was detected in tumors, but not in the other organs by a polymerase chain reaction analysis. From these experiments, we concluded that mtHSV should be a safe and promising oncolytic agent for cancer treatment.
Cancer gene therapy,, Herpes simplex virus-1,, icp34., 5,, Oncolysis,, Sarcoma
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【期刊论文】Link of a new type of apoptosis-inducing gene ASY/Nogo-B to human cancer
齐义鹏, Qin Li, , Bing Qi, Kiyomasa Oka, Misuzu Shimakage, Naohisa Yoshioka, Hirokazu Inoue
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-1年11月30日
Although apoptosis plays an essential role in the embryogenesis and homeostasis of multicellular organ-isms, this mechanism has not yet been fully clarified. We isolated a novel human apoptosis-inducing gene, ASY, which encodes an endoplasmic reticulum-targeting pro-tein without any known apoptosis-related motifs. This gene is identical to the Nogo-B, a splice variant of the Nogo-A which has recently been shown to be an inhibitor of neuronal regeneration in the central nervous system. Ectopic expression of the ASY gene led to extensive apoptosis, particularly in cancer cells. Furthermore, transcription of the ASY gene was suppressed in small cell lung cancer. These results suggest that a new type of apoptosis-inducing gene, namely, ASY, may be involved in the development of certain types of cancer. Oncogene (2001) 20, 3929-3936.
ASY, Nogo-B, apoptosis, cancer, endoplas-mic reticulum
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齐义鹏, Xiaoling Qu, Yipeng Qi, * and Bing Qi
X. Qu et al./Archives of Biochemistry and Biophysics 400(2002)233-244,-0001,():
-1年11月30日
hap, a novel human apoptosis-inducing gene, was identified to have two major mRNA species of 1.8 and 2.7kb in length by Northern blot analysis of poly(A)þ RNA from multiple human tissues. The two hap transcripts derive from the alternative polyadenylation site selection: an AATAAA signal at position 1528-1533 nt for the 1.8kb mRNA, and an AATAAA signal at position 2375-2380 nt for the 2.7kb mRNA. The 30-UTR spanning the region between the second and the third polyadenylation site of 2.7kb hap was demonstrated to exert a translational activation function for hap itself and the reporter gene chloramphenicol acetyltransferase (CAT) expression by approximately threefold, despite no differences observed in the steady-state level of relative cytoplasmic mRNA. Comparing the mRNA stability of two hap transcripts indicated that the longer mRNA was not more stable than the short one. Taken together, all these data provide evidence that the hap 30-UTR containing within the second and the third polyadenylation signal can regulate gene translation rather than transcription and mRNA stability.
Apoptosis-inducing gene hap, Polyadenylation signal, 30 untranslated region (, 30-UTR), , Translational activation
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