李克安
生物大分子(蛋白质、核酸、糖类物质等)与小分子探针(试剂、染料、药物等)的超分子作用及其在生物大分子测定方面的应用的研究,分子光谱分析,离子色谱法,分子印记聚合物的合成及应用,纳米微盒的合成及分析应用的研究等。
个性化签名
- 姓名:李克安
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学术头衔:
博士生导师
- 职称:-
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学科领域:
分析化学
- 研究兴趣:生物大分子(蛋白质、核酸、糖类物质等)与小分子探针(试剂、染料、药物等)的超分子作用及其在生物大分子测定方面的应用的研究,分子光谱分析,离子色谱法,分子印记聚合物的合成及应用,纳米微盒的合成及分析应用的研究等。
李克安,北京大学化学与分子工程学院教授,博士生导师,兼任北京大学副教务长。中国高等教育学会评估分会副理事长,全国高等理科教育研究会秘书长,全国高等学校教学研究理事会常务理事兼化学专业委员会副主任,教育部非化学化工类专业化学基础课教学指导委员会委员。“高等理科教育”副主编,“中国大学教学”编委,“化学通报”编委,“分析科学学报”编委,“北京大学教育评论”编委。科研方向:生物大分子(蛋白质、核酸、糖类物质等)与小分子探针(试剂、染料、药物等)的超分子作用及其在生物大分子测定方面的应用的研究,分子光谱分析,离子色谱法,分子印记聚合物的合成及应用,纳米微盒的合成及分析应用的研究等。主持了4项国家自然科学基金资助课题(“光散射技术原理及在生命物质分析中的应用研究”、“液膜分离在分析化学中的应用研究”、“磷酸锆纳米微盒的合成及其在生命物质研究中应用的研究”等)和2项省市级自然科学基金资助课题(“改性硅胶的制备及在痕量物质分离中的应用”、“糖原的分析方法及食管癌的诊断的研究”),参加了2项国家自然科学基金资助的重大课题和1项国家自然科学基金资助课题的研究。在国内外核心期刊上发表科学研究论文200余篇,其中近百篇被SCI收录,还在学术会议上发表论文60余篇。主编和参编出版了9本著作。科研成果获得中国分析测试协会科学技术奖(CAIA奖)一等奖两次、二等奖3次。2001年获宝钢教育奖特等奖。本课题组首次将共振光散射(瑞利光散射)应用到生物大分子与小分子探针作用的研究上,在国内外产生重要影响,2002年获教育部自然科学一等奖。担任主干基础课“分析化学系列课程”的主持人兼主讲教师,该系列课程于2003年被评为国家精品课程,同时为教育部创建理科名牌课程的优秀创建项目。获国家教学成果二等奖1项,北京市教学成果奖一等奖3项,二等奖1项。
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成果数
15
李克安, RongHua Yang a, *, Ying Zhang, Ke'An Li a, Feng Liu a, WingHong Chan b
Analytica Chimica Acta 525 (2004) 97-103,-0001,():
-1年11月30日
A pyrene-functional fluoroionophore, 1 was used to construct a supramolecular 1//-CD complex for Cu2+ recognition in water. In aqueous λ/-CD solution, 1 exhibits pyrene monomer fluorescence emission at 378 nm and 397nm, while in the presence of Cu2+, it shows a pyrene excimer emission at 452 nm with a decrease in the monomer fluorescence due to the formation of a 1: 2 metal-liganded complex. Based on the response characteristics of the supramolecular complex, a fluorescent ratiometric method was performed for the determination of Cu2+concentration in water. With the optimum conditions described, Cu2+ in aqueous solution can be determined from 1.2×10-6 to 4.5×10-4M. The Cu2+ selectivity of the complex is excellent, and the excimer fluorescence enhancements are very smaller induced by other heavy metal and transition metal ions.
Molecular recognition, Fluorescence, Ratiometry, Metal ion.,
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李克安, Ronghua Yang a, Ke'an Li a, *, Kemin Wang b, Feng Liu a, Na Li a, Fenglin Zhao a
Spectrochimica Acta Part A 59 (2003) 153-161,-0001,():
-1年11月30日
The supramolecular interactions of b-cyclodextrin(b-CD) and four kinds of alkylated b-cyclodextrin (b-CDs), i.e. heptakis (2,6-di-O-isobutyl)-b-cyclodextrin (Ob-b-CD), heptakis (2,6-di-O-n-octyl)-b-cyclodextrin (Oc-b-CD), heptakis(2,6-di-O-n-dodecyl)-b-cyclodextrin (Od-b-CD) and heptakis (2,6-di-O-n-hexadecyl)-b-cyclodextrin (Oh-b-CD) with tetrakis(4-methoxylphenyl)porphyrin (TMOPP) have been investigated by Rayleigh light scattering (RLS) technique. b-CDs form 2:1 inclusion complex with TMOPP following an obvious RLS enhancement of TMOPP. The inclusion abilities of different b-CDs were compared. The results show that the inclusion ability of b-CDs is related to the size of the alkylated substituent. Thus, a new mechanism of inclusion interaction has been proposed. The exact stoichiometric ratios and the association constants of the inclusion complexes have been examined by application of curve fitting method.
Supramolecular interaction, Porphyrin, Cyclodextrin, Inclusion complex, Rayleigh light scattering
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李克安, Lina Geng, Tao Bo, Huwei Liu, Na Li, &, Feng Liu, Kean Li, Junling Gu, Ruonong Fu
Chromatographia 2004, 59, January (No.1/2),-0001,():
-1年11月30日
A new capillary coating of alternate c-zirconium phosphate lysozyme fabricated for opentubular electrochromatography enantioseparation. The chiral compounds D, L-tryptophan tested were enantiomerically resolved. The influences brought by experimental parameters, such as organic modifier, temperature, applied voltage and pH of running buffer on the performance of the column, were investigated.
Capillary electrochromatography Open-tubular electrochromatographic enantioseparation Layer-by-layer assembly c-Zirconium Phosphate/, Lysozyme multilayer
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李克安, Lei Li, Feng Liu*, Xiangxu Kong, Shun Su, Ke An Li
Analytica Chimica Acta 452 (2002) 321-328,-0001,():
-1年11月30日
Based on the investigation of a non-ionic surfactant-salt-H2O liquid-liquid extraction system, general rules concerning salt selection are concluded and the mechanism of phase separation is explained. The extraction behavior of chlorpromazine hydrochloride (CPZ) and procaine hydrochloride (PCN) in such a system is studied. Research shows that the extraction efficiency of CPZ can amount to 96% by twice extraction, which means that quantitative extraction is realized, while that of PCN is 77%. This system produces distribution coefficients (KD) of 12.3 and 2.6, respectively, for CPZ and PCN. Extraction mechanism is deduced according to ultraviolet absorbance; and molecular fluorescence spectra change of the drugs in the system studied.
Non-ionic surfactant-salt-H2O extraction system, Polyethylene glycol-1000, Chlorpromazine hydrochloride, Procaine hydrochloride, Mechanism of extraction
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【期刊论文】The specificity of a chlorphenamine-imprinted polymer and its application
李克安, Wen Chen a, Feng Liu a, Xuemin Zhang b, Ke An Li a, *, Shenyang Tong a
Talanta 55 (2001) 29-34,-0001,():
-1年11月30日
A chlorphenamine-imprinted polymer was prepared in this study. Chromatographic analysis showed that the retentivity and selectivity of the imprinted polymer were greatly strengthened through molecular imprinting. As a consequence, chlorphenamine could be easily separated from diphenhydramine. Ionic interaction was proved to be the main power for the imprinted polymer to bind chlorphenamine. The strong extraction ability of the imprinted polymer for chlorphenamine in aqueous solution was evaluated further. It was shown that chlorphenamine, as low as 0.02mol 1−1, could be concentrated by 50 times with a recovery of more than 90% at pH 5. This study gave the potential of using the imprinted polymer for solid-phase extraction of practical samples.
Chlorphenamine-imprinted polymer, Solid-phase extraction, Molecular imprinted polymer (, MIP),
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李克安, Tieli Zhang, Feng Liu*, Wen Chen, Jun Wang, Kean Li
Analytica Chimica Acta 450 (2001) 53-61,-0001,():
-1年11月30日
Three molecularly imprinted polymers (MIPs) were prepared corresponding to three structurally related template compounds 4-hydroxybenzoic acid (4-HBA), gentisic acid (GA) and salicylic acid (SA) that differ in intramolecular hydrogen bonding ability using acrylamide (AA) as a functional monomer. HPLC methodwas used to evaluate the binding performances of the MIPs to the templates and several analogues. The results showed that the difference in their molecular recognition ability was pronounced. The highest molecular recognition ability was observed for 4-HBA-imprinted polymer. It was proved that the hydrogen bond interaction between the functional monomer and the template (4-HBA) played a major role in the recognition process and Scatchard analysis showed that two classes of binding sites were formed in 4-HBA-imprinted polymer. Their dissociation constants were estimated to be 1.76×10−4 and 1.40×10−3mol l−1, respectively. But for GA-or SA-imprinted polymer the molecular recognition ability was not improved compared to the blank polymer (BP). By comparison of the structures of the three templates, it was concluded that the molecular recognition ability will decrease when the template itself is able to form intramolecular hydrogen bond in the molecular imprinting process. This study will be helpful for us to understand the molecular recognition mechanism of MIPs and of instructive significance for the prediction of the selectivity of MIPs.
Hydroxybenzoic acid, Molecular imprinting, Molecular recognition, Intramolecular hydrogen bond
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【期刊论文】Molecular recognition of procainamide-imprinted polymer
李克安, Wen Chen, Feng Liu, Yitie Xu, Ke An Li, Shenyang Tong
Analytica Chimica Acta 432 (2001) 277-282,-0001,():
-1年11月30日
A procainamide-specific polymer was prepared by molecular imprinting using methacrylic acid as a functional monomer. It was showed that the imprinted polymer was capable of recognizing the functional difference of amide group and ester group between procainamide and procaine. The procaine-imprinted polymer was also prepared using the same monomer in the same molar ratio of monomer/template, however, it had no such ability. The different molecular imprinting effect of the two molecules showed that changing an amide group for an ester made quite a difference in terms of hydrogen acceptor. The role of the functional groups of the templates in the formation of complementary interacting sites in the polymer and the role of the corresponding interacting sites in the subsequent molecular recognition, was carefully discussed. Accordingly, the mechanism of molecular recognition of this system was proposed. Acrylamide was also used as a functional monomer for the preparation of procainamide-imprinted polymer to be compared with methacrylic acid. It was confirmed that hydrogen bond played an important role in the selectivity of the imprinted polymers.
Procainamide, Procaine, Molecular imprinting, Molecular recognition
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109浏览
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李克安, Wei Xing Ma, Feng Liu, Ke An Li, Wen Chen, Shen Yang Tong
Analytica Chimica Acta 416 (2000) 191-196,-0001,():
-1年11月30日
This paper reports a simple and highly selective method for the preconcentration and separation of trace Hg(Ⅱ) with aminopropylbenzoylazo-2-mercaptobenzothiazole bonded to silica gel (ABMBT SG). ABMBT SGwas tested for the selective extraction of Hg(Ⅱ) and it showed an exchange capacity of 41.4mmol g−1. After preconcentration and separation using an ABMBT SG column, trace Hg(Ⅱ) in environmental samples can be determined by UV-VIS spectrophotometry. The chromatographic column packed with ABMBT SG can be reused. The method is simple and efficient, and is applied to polluted soil (2mg g−1), incinerated biological material (9 or 18 ng g−1) and spiked natural water (5mg l−1).
Aminopropylbenzoylazo-2-mercaptobenzothiazole bonded to silica gel, Preconcentration, Separation, Hg(, Ⅱ), , Environmental samples, UV-VIS spectrophotometry
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【期刊论文】Studies on a high encapsulation of colchicine by a niosome system
李克安, Yongmei Hao, Fenglin Zhao, Na Li, Yanhong Yang, Ke'an Li*
International Journal of Pharmaceutics 244 (2002) 73-80,-0001,():
-1年11月30日
To prepare niosomes which have high encapsulation capacity for soluble drugs, starting from Span 60 and cholesterol, an improved method, evaporation-sonication method, was proposed. The corresponding niosomes show a good stability at least 40 days. Colchicine was chosen as a model drug for examining the capsula ion capacity of these niosomes. To obtain the highest encapsulation efficiency, several factors including the structure of surfactant, level of lipid, content of drug and cholesterol were investigated and optimized. The inner cause was also discussed. The results indicate that the Span 60 is the most ideal surfactant among four kinds of Span. Furthermore, the release studies of colchicine and 5-fluorouracil (5-FU) in vitro from niosomes exhibited a prolonged release profile as studied over a period of 24 h. The results demonstrated that niosomes prepared in this way not only have high encapsulation capacity but also is expected that side effects of drugs may be reduced. It still suggests that this method may be used extensively in the field of encapsulation soluble drugs.
Drug carrier, Niosome, Colchicine, 5-Fluorouracil, Span 60, Cholesterol
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