王占友
目前主要从事神经退行性疾病发病机理和天然药物治疗等领域的研究。
个性化签名
- 姓名:王占友
- 目前身份:
- 担任导师情况:
- 学位:
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学术头衔:
博士生导师, 教育部“新世纪优秀人才支持计划”入选者
- 职称:-
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学科领域:
人体解剖学
- 研究兴趣:目前主要从事神经退行性疾病发病机理和天然药物治疗等领域的研究。
王占友,教授,男,满族。1966年1月出生。现任中国医科大学细胞工程学教授、博士生导师。辽宁省解剖学会副理事长,辽宁省电子显微学会常务理事。获得教育部新世纪优秀人才支持计划、人事部高层次留学人才回国工作资助、辽宁省高等学校优秀人才支持计划基金资助,入选辽宁省新世纪百千万人才工程百人层次。分别于1992年和1997年在中国医科大学获得学士和硕士学位。2002年9月在瑞典哥德堡大学获得博士学位。2003年1月回国工作,被中国医科大学破格晋升为教授。
目前主要从事神经退行性疾病发病机理和天然药物治疗等领域的研究。先后在Neurobiology of Aging、Neuroscience、Brain Research等期刊发表原创论文20余篇。出席包括美国神经科学年会在内的国际会议10余次。获第16届国际解剖学会议(IFAA)青年研究者奖(YIA)。主持多项国家自然科学基金项目。
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15
【期刊论文】Abundant expression of zinc transporters in Bergman glia of mouse cerebellum
王占友, Zhan-You Wang a, *, Meredin Stoltenberg b, Liping Huangd, Gorm Danscher b, Annica Dahlstr
Z.-Y. Wang et al./Brain Research Bulletin 64 (2005) 441~448,-0001,():
-1年11月30日
Zinc transporters (ZnTs) are membrane proteins involved in zinc ion transportation in mammalian cells. Seven members of ZnT family, ZnT1-7, have been cloned and characterized. These transporter proteins have different cellular and sub-cellular locations, suggesting that they may play different roles in zinc homeostasis in normal and pathological conditions in different tissues. Cerebellum is one of the most zincenriched regions in the central nervous system, but little is known about zinc metabolism in the cerebellum. In the present study, we investigated the detailed distributions of four members (ZnT1, ZnT3, ZnT4 and ZnT6) of the ZnT family, in the mouse cerebellum. Immunostaining and confocal microscopic observations revealed a similar staining pattern of ZnTs in the molecular layer and the Purkinje cell layer. Double labeling with anti-S-100 or anti-MAP2 and anti-ZnTs clearly showed that the Bergman glial cell bodies in the Purkinje cell layer and their radial processes in the molecular layer exhibited strong immunofluorescence of all the tested ZnTs. However, the somata of the Purkinje cells contained a moderate immunostaining for ZnT1, but virtually lack of other three ZnTs. In the granular layer, ZnTs appeared with different immunostaining patterns. ZnT1 was expressed in a small number of neuronal cell bodies and their primary dendrites, whereas ZnT3 and ZnT4 were present in nerve terminals but not in the neuronal somata. ZnT6 was undetectable in either the cell bodies or processes in the granular layer. The present results indicate that the Bergman glial cells may play an important role in zinc metabolism in the mouse cerebellar cortex.
Astrocyte, Autometallography (, AMG), , Confocal laser scanning microscopy, Immunohistochemistry, S-100, Zinc transporter
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【期刊论文】Immunocytochemical localization of zinc transporter 3 in the ependyma of the mouse spinal cord
王占友, Gorm Danscher a, Zhanyou Wang b, Yong Kuk Kim c, Sung Joo Kim c, Yuanjie Sun c, Seung Mook Jo c, *
G. Danscher et al./Neuroscience Letters 342 (2003) 81~84,-0001,():
-1年11月30日
We report, for the first time, the light microscopical and ultrastructural appearance of ZnT3-immunoreactivities in the ependymal cells of the central canal of the mouse spinal cord. Light microscopy revealed the presence of ZnT3-immunoreactive (Ir) ependymal cells in 1 mm thick epon sections stained by the ABC method. The ZnT3-Ir cells were observed at all levels of the spinal cord, but were a little more numerous in lumbosacral segments than in cervicothoracic segments. The ZnT3-Ir cells had large, ovoid nuclei with abundant cytoplasm, and protruded into the lumen of the central canal. Our ultrastructural findings suggest that the ZnT3-Ir ependymal cells possess secretory activity directed towards the central canal. We propose that they may play a role in the trans-ependymal mechanism responsible for zinc homeostasis between cerebrospinal fluid and the central area of the gray matter.
Zinc, ZnT3, Immunocytochemistry, Ependyma, Spinal cord, Mouse
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王占友, Zhanyou Wang a, b, Gorm Danscher b, Yong Kuk Kim c, Annica Dahlstrom a, Seung Mook Jo c, *
Z. Wang et al./Neuroscience Letters 321 (2002) 37~40,-0001,():
-1年11月30日
In the present study, we showed for the first time the presence of inhibitory zinc-enriched neuron terminals in the mouse cerebellar cortex by means of double-immunohistochemistry for zinc transporter 3 (ZnT3) and glutamate decarboxylase (GAD). The co-localization of ZnT3 and GAD in the cerebellar cortex was analyzed by confocal microscopy. Strong, punctuate ZnT3-immunoreactivity (Ir) was predominantly distributed in the granule cell layer, while GAD-Ir was seen throughout the cerebellar cortical layers. All of the ZnT3-immunoreactive structures were also immunopositive to GAD, but not vice versa. Based on size and position, these double-labeled elements were axonal terminals of the Golgi and basket cells, in the granule cell and molecular layers, respectively. Observations by electron microscopy revealed that ZnT3-immunoreactive terminals showed typical characteristics of the inhibitory synapses like the following: (1) presynaptic terminals containing flat vesicles; and (2) symmetrical synaptic contacts with dendritic elements. The present results indicate that a zinc-containing GABAergic system exists in the mouse cerebellar cortex.
Inhibitory zinc-enriched neuron terminals, Double-immunohistochemistry, Confocal microscopy, Zinc transporter 3, Glutamate decarboxylase, Cerebellum, Mouse
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王占友, Zhan-You Wang, , CA, Jia-Yi Li, Emilio Varea, Gorm Danscher and Annica Dahlstrom
,-0001,():
-1年11月30日
Axonal transport of endogenous zinc ions in the rat sciatic nerve was studied by a stop-flow/nerve crush technique combined with zinc selenide autometallography (ZnSeAMG) at light and electron microscopic levels. Distinct accumulations of ZnSeAMG grains were detected, in particular proximal but also distal to the crushes, 1.5h after the operation, and the amounts of zinc ions increased further in the following 3-8 h. Ultrastructurally, ZnSeAMG grains were located predominantly in unmyelinated axons. The data suggest that a subpopulation of sciatic nerve axons contains and ransports zinc ions both antero- and retrogradely, indicating that the second neuron in the sympathetic nervous system is zinc enriched (ZEN). Neuro-Report 12: 2247-2250
Autometallography, Axonal transport, Crush, Rat, Sciatic nerve, Zinc, Zinc-enriched (, ZEN), neuronal pathway
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【期刊论文】Research report Retrograde tracing of zinc-enriched (ZEN) neuronal somata in rat spinal cord
王占友, Zhanyou Wang a, b, Gorm Danscher a, *, Seung Mook Jo a, Yuxiu Shi b, Henrik Daa Schr
Z. Wang et al./Brain Research 900 (2001) 80~87,-0001,():
-1年11月30日
The zinc selenide autometallographic (ZnSeAMG) technique for tracing the retrograde axonal transport of zinc ions in zinc-enriched (ZEN) neurons was used to map the distribution of ZEN neuronal somata in rat spinal cord. After a local injection of sodium selenide into AMG the dorsal or ventral horn, ZnSe-labeled ZEN neurons appeared in Rexed's laminae V, VII and X while laminae I and II were void. A few scattered ZEN somata were observed in the remaining laminae. The labeled neurons differed in shape and size, and the relatively high level of labeled somata around the injection site suggests that many ZEN neurons have relatively short axons or boutons en passage close to the neuronal origin. Ultrastructurally, the retrogradely transported zinc selenide clusters were found in the lysosomes of ZEN somata and proximal dendrites. Electron microscopic studies also revealed two different kinds of ZEN terminals: (1) terminals with flat synaptic vesicles making symmetric synaptic contacts; and (2) terminals with round vesicles making asymmetric synaptic contacts. The present study suggests the existence of propriospinal systems of ZEN neurons comprising both segmental and intersegmental ZEN connections and having either inhibitory or excitatory ZEN terminals. The ZEN neurons seem to form a vast network of terminals located primarily in the gray matter, but also contacting dendrites radiating into the white matter. Important functions of this rather massive system of ZEN terminals can not be deduced from our present knowledge, but the systems appear to be involved in both motor and sensory functions.
Zinc-enriched (, ZEN), , Retrograde transport, Zinc selenide autometallography, (, ZnSe), , Spinal cord, Rat, Somata
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王占友, Hong Zhu, Zhan-You Wang, Hans-Arne Hansson*
H. Zhu et al./Brain Research 977 (2003) 180~189,-0001,():
-1年11月30日
Proliferating cells are hardly detectable in the adult mammalian brain by microscopy of stained sections, but after pre-labeling with radioactive thymidine or 59-bromo-2-deoxyuridine (BrdU), either marks the nucleus, as do mitosis-related proteins such as Ki67 and PCNA. Engineered virus may also be used to mark proliferating cells. One alternative approach is to use the enzyme ribonucleotide reductase (RNR), expressed by proliferating cells, but not by quiescent ones. A monoclonal antibody against the M1 subunit of RNR was used to visualize proliferating cells in the brains of adult normal rats, rabbits, pigs and sheep. Stem cells were distinctly outlined. In the subgranular layer in the hippocampal dentate gyrus, most RNR immunoreactive cells were bipolar to multipolar, and had a large cell body and long processes. Two different populations of RNR expressing cells were visualized in the subventricular zone in the forebrain, one dominated by small, bipolar cells extending into the rostral migratory stream, while the other was formed by large multipolar cells, adjacent to the ependyma, with processes extending to the lateral ventricle. Furthermore, rare RNR-expressing cells were recognized throughout the brain. The RNR immunoreactive cells were immature, as they did not express any marker characterizing differentiated neurons and glial cells, except for a fraction that co-expressed the gliofibrillary acidic protein. BrdU and RNR were co-localized in proliferating cells in animals pretreated with BrdU. We conclude that RNR immunohistochemistry can accurately visualize proliferating cells, including stem cells, in adult mammalian brains. The occurrence of processes at cell proliferation is elucidated. Further, the advocated approach does not require any pre-labeling, and can be carried out on fixed tissues.
Ribonucleotide reductase, Stem cells, Neural progenitors, Radial glial cells, Adult brain and spinal cord, Immunohistochemistry
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【期刊论文】Research report Zinc-enriched GABAergic terminals in mouse spinal cord
王占友, Zhanyou Wang a, b, c, Jia-Yi Li a, Annica Dahlstrom a, Gorm Danscher b, *
Z. Wang et al./Brain Research 921 (2001) 165~172,-0001,():
-1年11月30日
Electrophysiological experiments have shown that zinc ions modulate glutamate and GABA receptors in brain slices. All the zinc-enriched neuronal pathways in the brain analyzed up until now have been found to be glutaminergic. Many years ago, zinc-enriched terminals with flat vesicles and symmetric synapses were found to be present in rat spinal cord by Henrik Daa Schr
Zinc, Zinc transporter, GAD, GABA, Neuromodulator, Terminal, Spinal cord
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【期刊论文】Abundant expression of zinc transporters in Bergman glia of mouse cerebellum
王占友, Zhan-You Wang a, *, Meredin Stoltenberg b, Liping Huang d, Gorm Danscherb, Annica Dahlstrom c, Yuxiu Shi a, Jia-Yi Li c
Brain Research Bulletin 64(2005)441-448,-0001,():
-1年11月30日
Zinc transporters (ZnTs) are membrane proteins involved in zinc ion transportation in mammalian cells. Seven members of ZnT family,ZnT1-7, have been cloned and characterized. These transporter proteins have different cellular and sub-cellular locations, suggesting that theymay play different roles in zinc homeostasis in normal and pathological conditions in different tissues. Cerebellum is one of the most zincenrichedregions in the central nervous system, but little is known about zinc metabolism in the cerebellum. In the present study, we investigatedthe detailed distributions of four members (ZnT1, ZnT3, ZnT4 and ZnT6) of the ZnT family, in the mouse cerebellum. Immunostaining andconfocal microscopic observations revealed a similar staining pattern of ZnTs in the molecular layer and the Purkinje cell layer. Doublelabeling with anti-S-100 or anti-MAP2 and anti-ZnTs clearly showed that the Bergman glial cell bodies in the Purkinje cell layer and theirradial processes in the molecular layer exhibited strong immunofluorescence of all the tested ZnTs. However, the somata of the Purkinje cellscontained a moderate immunostaining for ZnT1, but virtually lack of other three ZnTs. In the granular layer, ZnTs appeared with differentimmunostaining patterns. ZnT1 was expressed in a small number of neuronal cell bodies and their primary dendrites, whereas ZnT3 and ZnT4were present in nerve terminals but not in the neuronal somata. ZnT6 was undetectable in either the cell bodies or processes in the granularlayer. The present results indicate that the Bergman glial cells may play an important role in zinc metabolism in the mouse cerebellar cortex.
Astrocyte, Autometallography (, AMG), , Confocal laser scanning microscopy, Immunohistochemistry, S-100, Zinc transporter
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【期刊论文】Is the postganglionic sympathetic neuron zinc-enriched? A stop-
王占友, Zhan-You Wang, , CA Jia-Yi Li, Emilio Varea, Gorm Danscher and Annica Dahlstro
ZINC IONS IN CRUSHED SYMPATHETIC AXONS NEUROREPORT Vol 12 No 10 20 July 2001,-0001,():
-1年11月30日
Axonal transport of endogenous zinc ions in the rat sciaticnerve was studied by a stop-
Autometallography, Axonal transport, Crush, Rat, Sciatic nerve, Zinc, Zinc-enriched (, ZEN), neuronal pathway
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【期刊论文】Retrograde tracing of zinc-enriched (ZEN) neuronal somata in rat spinal cord
王占友, Zhanyou Wang, Gorm Danscher, Seung Mook Jo, Yuxiu Shi, Henrik Daa Schr
Brain Research 900(2001)80-87,-0001,():
-1年11月30日
AMG The zinc selenide autometallographic (ZnSe ) technique for tracing the retrograde axonal transport of zinc ions in zinc-enriched(ZEN) neurons was used to map the distribution of ZEN neuronal somata in rat spinal cord. After a local injection of sodium selenide intoAMG the dorsal or ventral horn, ZnSe -labeled ZEN neurons appeared in Rexed's laminae V, VII and X while laminae I and II were void. Afew scattered ZEN somata were observed in the remaining laminae. The labeled neurons differed in shape and size, and the relatively highlevel of labeled somata around the injection site suggests that many ZEN neurons have relatively short axons or boutons en passage closeto the neuronal origin. Ultrastructurally, the retrogradely transported zinc selenide clusters were found in the lysosomes of ZEN somataand proximal dendrites. Electron microscopic studies also revealed two different kinds of ZEN terminals: (1) terminals with flat synapticvesicles making symmetric synaptic contacts; and (2) terminals with round vesicles making asymmetric synaptic contacts. The presentstudy suggests the existence of propriospinal systems of ZEN neurons comprising both segmental and intersegmental ZEN connectionsand having either inhibitory or excitatory ZEN terminals. The ZEN neurons seem to form a vast network of terminals located primarily inthe gray matter, but also contacting dendrites radiating into the white matter. Important functions of this rather massive system of ZENterminals can not be deduced from our present knowledge, but the systems appear to be involved in both motor and sensory functions.
Zinc-enriched (, ZEN), , Retrograde transport, Zinc selenide autometallography, (, ZnSe), , Spinal cord, Rat, Somata
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