王建龙
主要研究领域为水污染控制、环境生物技术。
个性化签名
- 姓名:王建龙
- 目前身份:
- 担任导师情况:
- 学位:
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学术头衔:
博士生导师
- 职称:-
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学科领域:
环境科学技术基础学科
- 研究兴趣:主要研究领域为水污染控制、环境生物技术。
王建龙,男,1964年出生,现任教于清华大学,任教授、博士生导师。主要研究领域为水污染控制、环境生物技术。承担多项国家自然科学基金项目、国家科技攻关项目、“863”基金项目、国际科学基金以及国际和国内合作课题。曾获国家科学技术进步奖二等奖、中国科学院自然科学奖一等奖和北京市科学技术奖一等奖。在国内外学术期刊上发表论文100多篇,主要代表作有《生物固定化技术与水污染控制》、《现代环境生物技术》等。任《环境科学学报》、《Process Biochemistry》等期刊编委。
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1273
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成果数
16
【期刊论文】用氧吸收速率(OUR)表征活性污泥硝化活性的研究
王建龙, 吴立波, 齐星, 钱易
环境科学学报,1999,19(3):225~229,-0001,():
-1年11月30日
研究了用氧吸收速率(Oxygen Uptake Rate,简称OUR)表征活性污泥硝化活性的方法。利用生物抑制剂丙烯基硫脲(allylthiourea,简称ATU)和氯酸钠(NaClO3)可以选择性地抑制亚硝酸细菌和硝酸细菌的活性。通过测量不同时间的OUR,可以分别计算出污泥的亚硝化活性和硝化活性。结果表明:该方法的测量结果与实际反应器中的硝化反应情况相当一致。该方法简单易行,快速方便,适用于硝化系统中硝化活性的测量。
硝化活性, 氧吸收速率, 硝化, 生物抑制剂, 活性污泥
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王建龙, 王建龙**, W. Hegemann
中国科学(B辑),2003,33(1):47~53,-0001,():
-1年11月30日
从正在运行的流化床生物反应器中分离出对三氯酚具有高效脱氯能力的微生物群落,在含柠檬酸钛为氧化还原缓冲剂的合成培养基中研究了该群落的生理特性。结果表明,该微生物菌群可以利用丙酮酸为碳源和能源,丙酮酸发酵、硫酸盐还原和脱氯反应三个过程严格地按顺序依次进行,并且丙酮酸浓度在最初2d内急剧下降,同时有代谢产物乙酸、甲酸和氢气形成。只有当硫酸盐被还原成硫化物后,脱氯反应才会发生,硫酸盐和钼酸盐的存在会强烈地抑制微生物的脱氯活性。溴乙烷磺酸酯(BEs)可以完全抑制微生物的产甲烷活性。青霉素G(10μg/mL)对该微生物菌群的脱氯作用没有抑制,该微生物菌群按形态特征可以分为3类,即弧菌、链球菌和杆菌,利用荧光标记的16s和23s rRNA寡核苷酸探针进行原位杂交试验表明,该微生物菌群中存在Proteobacteria的r亚类和δ亚类。
氯酚, 生物降解硫酸盐还原菌, 产甲烷菌, 专性抑制剂, 持久性有机污染物
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209浏览
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王建龙, 张悦②, 李花子, 施汉昌, 竺建荣
,-0001,():
-1年11月30日
对生物传感器快速测定BOD的试验装置与测定方式进行了改进,改进后的测定系统具有良好的重现性和准确度。同时确定了最佳测定条件为温度30℃,pH5.0,固定化细胞15g。
BOD,, 生物传感器,, 试验装置,, 影响因素
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75浏览
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【期刊论文】固定化细胞降解2,42二氯酚的动力学及其对SBR系统强化效果研究
王建龙, 全向春, 施汉昌, 钱易
环境科学,2002,23,36~39,-0001,():
-1年11月30日
将降解2,42二氯酚(简称2,42DCP)的高效菌固定化后,研究了半连续流实验中固定化细胞降解活力的变化及2,42DCP降解的动力学,并比较了固定化高效菌强化的SBR系统与不加固定化高效菌的对照系统长期运行时对目标物的降解特性. 结果表明:固定化细胞的降解活性会在半连续流降解实验中得到恢复与增强,固定化细胞对2,42DCP 的降解遵循零级反应动力学。投加固定化菌的强化SBR系统较不加固定化菌的对照系统具有启动时间短,耐冲击负荷能力强的特点. 进水为混合基质或目标物非持续性存在下,长期运行中,生物强化系统保持了对目标物降解的特异性。
固定化细胞, 高效菌, 生物强化, 2,, 42二氯酚的降解动力学
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49浏览
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【期刊论文】High-carbohydrate wastewater treatment by IAL-CHS with immobilized Candida tropicalis
王建龙, Yongming Zhang a, Bruce E. Rittmann b, Jianlong Wang c, *, Yuhong Sheng d, Juntang Yue, Hanchang Shi d, Yi Qian d
Process Biochemistry 40 (2005) 857-863,-0001,():
-1年11月30日
Anovel immobilized-cell bioreactorwas developed and utilized for treatment of high-carbohydratewastewater and production of single-cell protein (SCP) simultaneously. The system is the internal airlift loop reactor with ceramic honeycomb support for biofilm (IAL-CHS), and the microorganisms tested was Candida tropicalis, which has a high protein yield. The optimum temperature for C. tropicalis was 35-40℃, and bioreactor experiments were performed at 35℃. The internal airlift loop reactor with ceramic honeycomb supports allowed a large and dense accumulation of biomass, but also generated significant SCP. Mass-transport resistance did not limit the overall COD-removal kinetics and oxygen mass transfer may have been accelerated to the biofilm. Fermentation of carbohydrates to organic acids lowered the pH below the optimum range of 4.5-6.5, and pH below 4.5 retarded or stopped the oxidation reactions for the organic acids. Since organic-acid oxidation is necessary for attaining COD removal from the wastewater, pH control may be essential for effective treatment in some cases with high-carbohydrate wastewaters. The addition of 0.35g/L urea could increase the COD removal rate. Continuous wastewater was carried out and the organic loading reached 15kg COD/(m3 day).
Airlift biofilm bioreactor, Ceramic honeycomb support, High-carbohydrate wastewater, Immobilized cells, Single-cell protein
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58浏览
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王建龙, 胡林林, 文湘华, 杨宁, 钱易
环境科学,2004,25(4):74~77,-0001,():
-1年11月30日
在SBR反应器中以醋酸钠为碳源,UASB厌氧颗粒污泥作为接种污泥,在好氧曝气条件下运行。通过观察污泥颗粒形态、结构等的变化,发现在运行中污泥颗粒经历了形态保持,成分置换的过程。污泥浓度先增加后降低,在运行35d后逐渐稳定在5g/L,SVI值稳定在30~40mL/g的水平。在40~60d内反应器中颗粒污泥一直占主体成分,悬浮相浓度低于0.5g/L。在好氧条件下最终颗粒污泥形态、大小稳定,表明好氧颗粒污泥已经成功获得,好氧颗粒污泥与接种污泥相比在粒径、沉降速度、含水率以及惰性成分的含量上都有一定的变化。 电镜观察还表明,原厌氧颗粒污泥中的微生物以球菌为主,而获得的好氧颗粒污泥中的微生物以丝状菌和杆菌为主。
厌氧颗粒污泥, 好氧颗粒污泥, SBR
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143浏览
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王建龙
生物化学与生物物理进展,2001,28(1):125~128,-0001,():
-1年11月30日
基于生物催化和免疫原理的生物传感器在环境领域中获得了广泛应用。近年来, 随着分子生物学和生物技术的发展,人们开发了以核酸探针为识别元件,基于核酸相互作用原理的DNA生物传感器。该传感器可用于受感染微生物的核酸序列分析、优先控制污染物的检测以及污染物与DNA之间相互作用的研究,在环境污染监测中具有潜在的巨大应用前景。简要介绍了核酸杂交生物传感器的基本原理及其在环境微生物和优先控制污染物(priority pollutant)检测中的应用研究进展。
DNA生物传感器,, 核酸杂交,, 环境监测,, 优先污染物
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【期刊论文】Study on performance characteristics of SBR under limited dissolved oxygen
王建龙, Linlin Hua, Jianlong Wang a, b, *, Xianghua Wen a, Yi Qian a
Process Biochemistry 40 (2005) 293-296,-0001,():
-1年11月30日
The performance of sequence batch reactor (SBR) was studied under four different air fluxes. Special attention was paid to the operating characteristics of SBR under limited aeration or low dissolved oxygen (DO) conditions. At the air flux of 40 l/h, COD and NH4+-N had been removed just before the cycle was over, and during the cycle DO was about 0.5mg/l most of the time. Operational parameters, such as DO, ORP and pH, were monitored during the whole cycle. The effect of these parameters on the removal efficiency of COD and NH4+-N was discussed.
SBR, Limited aeration, DO, NH4+, -N, pH, ORP
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40浏览
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王建龙, Hu Linlin b, Wang Jianlong a, b, *, Wen Xianghua b, Qian Yi b
Process Biochemistry 40 (2005) 5-11,-0001,():
-1年11月30日
The cultivation of aerobic granules in SBR by seeding anaerobic granular sludge was investigated using acetate-based synthetic wastewater. Morphological variation of granular sludge in the reactor was observed and results revealed that the inoculated anaerobic granules experienced a process of disintegration-recombination-growing up. The disintegrated anaerobic sludge may play a role of nucleus for the granulation of aerobic sludge. The sludge concentration increased for the first 4 weeks, then decreased and reached a stable value of 5g/l at 60d with a SVI of 30-40ml/g. Granular sludge dominated in the reactor and suspended sludge concentration was less than 0.5g/l at the end of the process. In the inoculated anaerobic granular sludge, spherical bacteria were the main microorganisms, however, rod-shaped and filamentous microorganisms prevailed in the cultivated aerobic granules. This is ready way to cultivate aerobic granules by seeding anaerobic granular sludge.
Aerobic granules, Granulation, Anaerobic granule, Microbial aggregates, SBR process
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【期刊论文】Microbial degradation of phthalic acid esters under anaerobic digestion of sludge
王建龙, Wang Jianlong*, Chen Lujun, Shi Hanchang, Qian Yi
Chemosphere41 (2000) 1245-1248,-0001,():
-1年11月30日
The microbial degradation by anaerobic sludge of three phthalates, priority pollutants, listed by both China National Environmental Monitoring Center and the U.S.EPA, namely, dimethyl (DMP), di-n-butyl (DBP) and di-noctyl (DOP) phthalates was investigated. The experimental results indicated that the biodegradation rate and the biodegradability of three phthalates under anaerobic conditions appeared to be related to the length of the alkyl-side chains. More than 90% of DMP and DBP with the short alkyl-side chain phthalates can be degraded, whereas the DOP degradation appeared to be relatively slow under the same experimental conditions. The quantity of methane produced was measured and the results showed that both the ester groups and the phthalate ring were mineralized at a signi
Phthalic acid esters, Biodegradation, Kinetics, Priority pollutant, Anaerobic degradation
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