李保新
化学发光和电化学发光分析以及流通式光学传感器
个性化签名
- 姓名:李保新
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学术头衔:
博士生导师
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学科领域:
分析化学
- 研究兴趣:化学发光和电化学发光分析以及流通式光学传感器
李保新 1971年生于陕西凤翔,1995年于陕西师范大学化学系获理学学士学位,1998年获分析化学理学硕士学位,2002年于西南师范大学化学化工学院获分析化学理学博士学位。2000年从助教越级晋升为副教授,成为陕西师范大学最年轻的副教授(29岁)。2003年越级晋升为教授,成为陕西师范大学最年轻的教授(32岁)。2002年获霍英东教育基金会第八界高等院校青年教师基金资助,并应邀作为获资助教师代表参加在人民大会堂举行的颁奖大会。2004荣获国家青年自然科学基金。参加一项国家自然科学基金资助项目和两项省部级自然科学基金资助项目。其研究成果获陕西省科技进步二等奖一项, 获2002年陕西省化学会青年化学论文一等奖。现为陕西师范大学化学与材料科学学院教师,硕士研究生导师。主要从事分析化学的教学和科研工作,其研究领域主要为化学发光和电化学发光分析以及流通式光学传感器。在Anal. Chem., Biosens. & Bioelectronics, Anal. Chim. Acta, Talanta等SCI源期刊上发表论文近30篇,其中影响因子3.0以上的论文两篇。
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287
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成果数
13
【期刊论文】Plant Tissue-Based Chemiluminescence Flow Biosensor for Glycolic Acid
李保新, Baoxin Li, Zhujun Zhang, * and Yan Jin
,-0001,():
-1年11月30日
A novel plant tissue-based chemiluminescence (CL) biosensor for glycolic acid combined with flow injection analysis is proposed in this paper. The spinach tissue acts as the molecular recognition element. Glycolic acid is oxidized by oxygen under the catalysis of glycolate oxidase in the tissue column to produce hydrogen peroxide, which can react with luminol in the presence of peroxidase of spinach tissue to generate a CL signal. The CL emission intensity was linear with glycolic acid concentration in the range of 4
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李保新, Baoxin Li, Zhujun Zhang*, Yan Jin
Biosensors and Bioelectronics 17 (2002) 585-589,-0001,():
-1年11月30日
A novel plant tissue-based chemiluminescence (CL) biosensor for dopamine combined with flow injection analysis is presented in this paper. The potato roots act as molecular recognition elements. Dopamine is oxidized by oxygen under the catalysis of polyphenol oxidase in the tissue column to produce hydrogen peroxide, which can react with luminol in the presence of peroxidase of potato tissue to generate CL signal. The CL emission intensity was linear with dopamine concentration in the range of 1×10-5-1×10-7g/ml and the detection limit was 5.3×10-8g/ml (3σ) with a relative standard deviation of 1.7%. Combined with microdialysis sampling, the biosensor was applied to monitor the variation of dopamine level in the blood of rabbit after the administration of dopamine to demonstrate the favorable resolution and reliability of the system for in vivo on-line monitoring.
Chemiluminescence, Plant tissue-based biosensor, Flow injection, Microdialysis, Dopamine
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李保新
,-0001,():
-1年11月30日
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30浏览
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107下载
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李保新
,-0001,():
-1年11月30日
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41浏览
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李保新, Baoxin Li, Zhujun Zhang*, Wei Liu
Talanta 54 (2001) 697-702,-0001,():
-1年11月30日
A simple and sensitive flow-injection chemiluminescence (CL) system for automated dissolution testing is described and evaluated for monitoring of dissolution profiles of isoniazid tablets. The undissolved suspended particles in the dissolved solution were eliminated via on-line filter. The novel CL system of KIO4-isoniazid was also investigated. The sampling frequency of the system was 120h−1. The dissolution profiles of isoniazid fast-release tablets from three sources were determined, which demonstrates the stability, great sensitivity, large dynamic measurin grange and robustness of the system.
Flow-injection analysis, Chemiluminescence, Dissolution testing, Isoniazid
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李保新, Baoxin Li, Zhujun Zhang*, Manli Wu
Analytica Chimica Acta 432 (2001) 311-316,-0001,():
-1年11月30日
A novel chemiluminescence (CL) flow system for the determination of sulfite is described. It is based on the direct CL reaction of sulfite and silver(Ⅱ) in acid media without any sensitizers. The unstable Ag(Ⅱ) is on-line electrogenerated via a positive Pt-electrode from AgNO3 in HNO3 medium by constant current electrolysis, resulting in the elimination of the inconvenience of its instability to water. The CL intensity emission intensity was linear with sulfite concentration in the range 4×10−7-1×10−5mol/l; the detection limit was 1.7×10−7mol/l (3). The system was applied satisfactorily to the determination of sulfite dioxide in air.
Flow injection, Chemiluminescence, Sulfite, On-line electrogenerated Ag(, Ⅱ),
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14浏览
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李保新, Baoxin Li, Zhujun Zhang*, Manli Wu
Talanta 51 (2000) 515-521,-0001,():
-1年11月30日
A novel chemiluminescence (CL) flow system for the determination of quinine is described. It is based on the direct chemiluminescence reaction of quinine and cobalt(Ⅲ) in sulfuric acid medium. The unstable Co(Ⅲ) was on-line electrogenerated by constant-current electrolysis. The chemiluminescence intensity was linear with a quinine concentration in the range of 0.1-100μg ml-1. The determination limit was 3.3×10-8g ml-1. The whole process could be completed in 1min. The proposed method is suitable for automatic and continuous analysis, and has been applied successfully to the analysis of quinine in pharmaceutical preparation.
Flow injection, Chemiluminescence, Quinine, On-line electrogenerated Co(, Ⅲ), , Pharmaceutical analysis
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19浏览
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李保新, Baoxin Li, Zhujun Zhang*, Lixia Zhao
Analytica Chimica Acta 468 (2002) 65-70,-0001,():
-1年11月30日
The novel flow chemiluminescence (CL) system for determination of pipemidic acid was proposed, which was based on the sensitizing effect of pipemidic acid on the CL oxidation of sulfite by KMnO4 in acid media. Combined with the technique of ultrafiltration, the flow-injection CL system was applied to study in vitro the bovine serum album (BSA) binding of pipemidic acid. The estimated association constant (K) and the number of the binding site (n) on one molecule of BSA were 8.81
Chemiluminescence, Drug-protein interaction, Ultrafiltration sampling, Flow injection
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22浏览
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李保新, Baoxin Li, Zhujun Zhang*, Lixia Zhao, Chunli Xu
Analytica Chimica Acta 459 (2002) 19-24,-0001,():
-1年11月30日
A novel chemiluminscence (CL) flow-through sensor for pipemidic acid is described. It was based on the sensitizing effect of pipemidic acid on the CL oxidation of sulfite by sodium bismuthate in H2SO4 media. The solid-phase sodium bismuthate was mechanicially immobilized on the sponge rubber inside of the CL flow cell as CL oxidant. The calibration graph is linear in the range 0.1-10g/ml with a detection limit of 6.2×10−8g/ml (3σ). A complete analysis couldbe performedin 1 min with a relative standard deviation (R.S.D.) of 2.5% for 2g/ml pipemidic acid (n=8). This methodhas been successfully applied to determine pipemidic acid in pharmaceutical preparation.
Chemiluminscence, Flow-through sensor, Pipemidic acid, Sodium bismuthate, Solid-phase oxidant
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15浏览
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李保新, Baoxin Li, Zhujun Zhang*, Lixia Zhao
Analytica Chimica Acta 445 (2001) 161-167,-0001,():
-1年11月30日
A novel chemiluminescence (CL) flow-through sensor for hydrogen peroxide is described. It is prepared by immobilizing hemoglobin (Hb) with the sol-gel method on the flow cell, and by electrostatically immobilizing luminol on strongly basic anion-exchange resin. Hydrogen peroxide is sensed by the CL reaction with Hb sensitive membrane and luminol bleeding from the ion-exchange column with immobilized luminol by hydrolysis. The calibration graph is linear in the range of 6×10−5 to 4×10−7 mol l−1 with a detection limit of 1.3×10−7 mol l−1 (3σ). A complete analysis could be performed in 1 min with a relative standard deviation of 1.7% for 5×10−6mol l−1 H2O2 (n=9). The sensor has been applied successfully to the determination of hydrogen peroxide in rainwater.
Chemiluminescence, Flow-through sensor, Sol-gel method, Hydrogen peroxide, Hemoglobin
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