金勇丰
从事分子生物学研究
个性化签名
- 姓名:金勇丰
- 目前身份:
- 担任导师情况:
- 学位:
-
学术头衔:
博士生导师, 教育部“新世纪优秀人才支持计划”入选者
- 职称:-
-
学科领域:
生物化学
- 研究兴趣:从事分子生物学研究
男,35岁,教授,博士,浙江大学生物化学研究所常务副所长。教育部“新世纪优秀人才支持计划”获得者,浙江省“新世纪151人才工程”培养人员。杭州全国现代分子生物学实验技术培训班主讲教师。1997年7月获浙江大学博士学位,毕业后留校从事分子生物学研究,2002-2003年在奥地利维也纳大学生物中心微生物和遗传研究所做访问学者。自1997年博士毕业以来,共主持了国家自然科学基金3项,国家教育部新世纪优秀人才支持计划(人口健康领域)1项,国家人事部留学人员科技活动项目择优资助经费1项,浙江省重点项目2项,浙江省自然科学基金4项,宁波市(浙大专项)重大项目1项等10余项科研项目;作为第二主持人和骨干参加了国家“九五”攻关项目2项,国家863重大专项1项,国家科技部农业转化资金1项,国家中小型企业创新基金1项等10余项科研项目;已获得国家技术发明二等奖1项,省部级二等奖2项,三等奖1项等多项奖励,并获得国家发明专利7 项,在《RNA》等国内外著名学术期刊发表学术论文60余篇。
-
主页访问
2494
-
关注数
0
-
成果阅读
162
-
成果数
4
【期刊论文】Isolation and Expression of a Wheat Pollen-Specific Gene with Long Leader Sequence
金勇丰, JIN Yong-Feng*, BIAN Teng-Fei
,-0001,():
-1年11月30日
Here we report the isolation and expression of a pollen-specific cDNA TaPSG719 with an unusually long 5' leader sequence via suppression subtractive hybridization and 5'/3' RACE techniques. The insert in the TaPSG719 is 1 172-bp long, and encodes a protein of 188 amino acids long with a pI of 12.1. This sequence did not show a significant homology to any genes deposited in the public database by BLAST search. Southern blot indicated that TaPSG719 might be multicopy. Northern blot and RT-PCR analyses indicated that TaPSG719 transcripts were specific for mature pollen, and undetectable in microspore, immature seed, stem, young leaf, root and ovary. During pollen development, TaPSG719 transcripts were first detectable on the 5th day before anthesis and increased rapidly at middle stages of pollen development with maximum levels on the 4th day before anthesis, and decreased during pollen maturation. It is noted that TaPSG719 contains an unusually long 5' leader sequence (329-nt) upstream the ATG start codon, suggesting that the gene could be subject to translational regulation. To investigate the role of the 5' UTR on translation, in vitro transcription/translation assays with various deletion and mutation constructs were performed using wheat germ extract. The results demonstrated that the 5' UTR affected positively downstream translation in wheat germ extract.
wheat (, Triticum aestivum), , pollen-specific cDNA, gene expression, suppression subtractive hybridization
-
34浏览
-
0点赞
-
0收藏
-
0分享
-
222下载
-
0评论
-
引用
金勇丰, YONGFENG JIN and TENGFEI BIAN
,-0001,():
-1年11月30日
A comparison of Arabidopsis DNA sequences revealed that the final nucleotides at the 3 end of approximately half of the Arabidopsis mRNAs, immediately upstream of the poly(A) tail, differ from the corresponding genomic sequences. This suggests that extra nucleotides were added to these mRNAs at their 3 termini prior to polyadenylation. Among the mRNAs containing additional nucleotides, approximately 65% had a single additional nucleotide, with the nucleotide C added most often. This nontemplated addition before the addition of the poly(A) sequence could be a major contributing factor to the often observed heterogeneity in transcription products. These findings should be helpful in the elucidation of the mechanisms of mRNA 3-end processing.
cleavage/, polyadenylation site, nontemplated nucleotides, 3', -end processing
-
38浏览
-
0点赞
-
0收藏
-
0分享
-
111下载
-
0评论
-
引用
【期刊论文】A novel tri-primer PCR method (TP-PCR) for rapid construction of fpg gene
金勇丰, Ying Xu Chen a, *, He Liu a, Wen Bo Zhang b, Yong Feng Jin b
Journal of Microbiological Methods 56(2004)359-364,-0001,():
-1年11月30日
A novel tri-primer polymerase chain reaction method (TP-PCR) was developed for the construction of a fused fpg gene, in which no endonuclease and ligase were used. Instead, two templates and three specifically designed primers were applied. Results showed that pheB and gfp genes, which encodes the catechol 2,3-dioxygenase and the green fluorescent protein (GFP), respectively, were successfully fused into an fpg gene through the rapid TP-PCR system, indicating that TP-PCR method could be a useful tool for DNA fragment fusion in which no proper endonuclease sites were available.
Tri-primer PCR (, TP-PCR), , Fused fgp gene, Catechol 2,, 3-dioxygenase, Green fluorescent protein
-
52浏览
-
0点赞
-
0收藏
-
0分享
-
418下载
-
0评论
-
引用
金勇丰, Yong-Feng JIN* and Teng-Fei BIAN
Acta Biochimica et Biophysica Sinica 2004, 36 (7): 467-476,-0001,():
-1年11月30日
A novel pollen-specific full-length cDNA clone PSG076 was isolated using suppression subtractive hybridization and 5'/3' RACE techniques. PSG076 was shown to exhibit multi-site polyadenylation by sequencing the 3' ends of the cDNAs. At least six transcripts with different length were produced from the single gene based on different poly(A) tail attachment sites. However, polyadenylation consensus sequence AAUAAA was not seen at the 3'-untranslated sequence. PSG076 contained a 299 bp 5' untranslated region and an open reading frame of 663 bp encoding a 221 amino acid peptide with pI of 4.31. A blast search revealed that this sequence did not show a significant similarity to any genes deposited in the public database. Southern blot indicated that PSG076 was a single copy gene. Northern blot and RT-PCR analysis indicated that PSG076 transcripts showed specific expression in mature pollen, and weak or undetectable signals in uninucleate microspore, immature seed, stem, young leave, root and ovary. Further analysis of the expression pattern in gametophyte showed that PSG076 transcripts were undetectable in uninucleate, binucleate microspore and pollen at early stage, and were first detectable and increased rapidly at middle and late stages of pollen development with the maximum level in mature pollen and also expressed in germinating pollen in vivo, suggesting that PSG076 might play a role in pollen germination and pollen tube growth in addition to its function in maturation. The evidences gathered in this work indicated that the six different transcripts from the single gene were differentially expressed during pollen development.
wheat (, Triticum aestivum L., ), , pollen-specific cDNA, suppression subtractive hybridization, multiple-site polyadenylation, differential expression
-
38浏览
-
0点赞
-
0收藏
-
0分享
-
291下载
-
0评论
-
引用