熊炜
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- 姓名:熊炜
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学术头衔:
博士生导师, 教育部“新世纪优秀人才支持计划”入选者
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学科领域:
光学
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熊炜,男,中南大学副研究员。全国优秀博士学位论文奖、教育部霍英东高校青年教师奖获得者,受教育部2004年度(首批)新世纪优秀人才支持计划资助。近5年主持国家自然科学基金、国家973前期研究专项基金等科研项目6项;参与其他国家级科研项目6项;发表论文40篇(SCI收录26篇,EI收录4篇),其中第一作者或通讯作者论文14篇(SCI收录9篇,EI收录3篇,单篇最高引用30次);作为副主编参编专著1本;申报国家发明专利4项。美国癌症研究联盟(American Association for Cancer Research, AACR)活跃会员(Active member)。
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11
熊炜
,-0001,():
-1年11月30日
Nasopharyngeal carcinoma (NPC) is a particularly common malignant disease in areas of south China and Southeast Asia. To characterize the gene expression profiling of NPC, we detected the gene expression profiles in 22 NPC and 10 nontumor nasopharyngeal epithelial tissues by complementary DNA microarray. We identified 503 genes that were significantly ( P b .001) differentially regulated between NPC and nontumor nasopharyngeal epithelial tissues. The differentially expressed genes are involved in many signaling pathways, such as the Wnt, transforming growth factor– b, and mitogen-activated protein kinase signaling pathways. The aberrant expression of the Wnt signaling pathway components, such as wingless-type MMTV integration site family, member 5A, Frizzled homolog 7, casein kinase IIb, b-catenin, CREB-binding protein, and Dishevelled-associated activator of morphogenesis 2 was validated on the NPC tissue microarrays. The data suggest that the Wnt signaling pathway may be abnormally regulated in NPC, which provides insight into the molecular mechanisms of NPC.
Nasopharyngeal Carcinoma, cDNA microarray, Tissue microarray
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熊炜
,-0001,():
-1年11月30日
Purpose Microarray analysis was used to bring a comprehensive insight into underlying molecular mechanisms and obtain a whole assessment of aberrant gene expression in nasopharyngeal carcinoma (NPC). Methods Combined with microdissection, gene expression proWles in 23 NPCs and 10 nontumor nasopharyngeal epithelial tissue samples were analyzed. Results Gene expression patterns suggested the dysregulation of the GTP/GDP-bound Ras cycle and an abnormal hyperactivity of cell cycle in NPC. Alterations in the WNT pathway suggest that this pathway may be activated in NPC. A 6-feature weighted-voting model was chosen because it represented the main characteristics of NPCs and predicted NPCs most accurately from the nontumor tissues (33 of 34 correct calls; 97.1% accuracy, Fisher’s exact test, P value = 8.389 X 10E8). Conclusions The data generated in this study represent a comprehensive list of genes aberrantly regulated in NPC. The 6-feature weighted-voting model may provide an extensive list of potential molecular markers for early diagnosis.
Nasopharyngeal carcinoma (, NPC), ,, cDNA microarray,, Microdissection
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熊炜, Zhaoyang Zeng, PhD, *, Yanhong Zhou, Wenling Zhang, Xiaoling Li, Wei Xiong, Huaying Liu, Songqing Fan, Jun Qian, Lili Wang, Zheng Li, Shourong Shen, BS, and Guiyuan Li
March 2006 Vol. 8 No.3,-0001,():
-1年11月30日
Purpose: Nasopharyngeal carcinoma (NPC) poses one of the serious health problems in southern Chinese, with an incidence rate ranging from 15 to 50/100,000. In our previously linkage analysis, a locus on 3p21 was identified to link to NPC. In this study, family-based association analysis was performed to test the transmission disequilibrium of chromosome 3p in 18 high-risk nasopharyngeal carcinoma families of Hunan province in southern China. Methods: Single locus and multi-point of transmission disequilibrium test was performed by Genehunter program package with 15 microsatellite markers on chromosome 3p in 18 nasopharyngeal carcinoma pedigrees. Results: A major transmission disequilibrium peak was observed near D3S1568, which possessed 20 alleles or haplotypes of 6 loci, spanning a 12.4 cM region from D3S1298 to D3S1289 on chromosome 3p21.31-3p21.2, and 3 alleles or haplotypes reached high significantly difference (P<0.01). Conclusion: These results reflected a link disequilibrium between this chromosome region and a nasopharyngeal carcinoma susceptibility locus, and provided further evidence that a novel nasopharyngeal carcinoma susceptibility gene may be located in this chromosome region. These alleles or haplotypes transmitting disequilibrium in nasopharyngeal carcinoma pedigrees may act as the highly risk molecular markers after verified in large population.
nasopharyngeal carcinoma, chromosome 3p, transmission disequilibrium test
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【期刊论文】3号染色体短臂上八个短串联重复序列位点的遗传多态性研究
熊炜, 曾朝阳, 熊芳, 沈守荣, 李小玲, 李伟芳, 王蓉, 范松, 青杨一新, 周厚德, 周鸣, 李桂源
中华医学遗传学杂志2003,20(5):413~416,-0001,():
-1年11月30日
目的研究湖南汉族人群8个位于染色体3p区域的短串联重复序列(short tandem repeat,STR)位点: D3S1297 D3S1489、D3S1266、D3S1568、D3S1289、D3S1300、D3S1285和D3S3681基因型及等位片段频率分布。方法 随机抽取225名湖南汉族无关个体静脉血,抽提DNA,复合PCR技术扩增上述位点,ABI 377全自动测序仪进行基因分型。结果 共检出91种等位基因,基因频率分布在0.002~0.431之间,构成312种基因型。8个sTR位点基因型分布均符合Hardy Weinberg平衡(p>0.05),杂台度大于0.729,个体识别力大于0.725,非父排除率大于0.596,多态信息含量大0.682。民族比较结果显示,湖南汉族与非洲黑人及欧洲白人在大多数位点差异有显著性(p<O.001)。结论研究结果对人类群体遗传学及法医学研究具有重要应用价值。
汉族, 短串联重复序列, 遗传多态性, 基因扫描
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【期刊论文】A Susceptibility Locus at Chromosome 3p21 Linked to Familial Nasopharyngeal Carcinoma
熊炜, Wei Xiong, Zhao Yang Zeng, Jia Hui Xia, Kun Xia, Shou Rong Shen, Xiao Ling Li, Dong Xu Hu, Chen Tan, Juan Juan Xiang, Jie Zhou, Hao Deng, Song Qing Fan, Wei Fang Li, Rong Wang, Ming Zhou, Shi Guo Zhu, Hong Bin Lu, Jun Qian, Bi Cheng Zhang, Jie Ru Wang, Jian Ma, Bing Yi Xiao, He Huang, Qiu Hong Zhang, Yan Hong Zhou, Xiao Min Luo, Hou De Zhou, Yi Xin Yang, He Ping Dai, Guo Yin Feng, Qian Pan, Ling Qian Wu, Lin He, and Gui Yuan Li
[CANCER RESEARCH 64, 1972-1974, March 15, 2004],-0001,():
-1年11月30日
Nasopharyngeal carcinoma (NPC) poses one of the serious health problems in southern Chinese, with an incidence rate ranging from 15 to 50/100,000. Chromosome translocation t(1;3) and frequent loss of heterogeneity on short arms of chromosome 3 and 9 have been reported to be associated with NPC, and a genome-wide scan identified an NPC susceptibility locus on chromosome 4p15.1-q12 recently. In our study, we collected samples from 18 families at high risk of NPC from the Hunan province in southern China, genotyped with a panel of polymorphic markers on short arms of chromosomes 3, 9, and 4p15.1-q12. A locus on 3p21 was identified to link to NPC with a maximum logarithm of odds for linkage score of 4.18. Fine mapping located the locus to a 13.6-cM region on 3p21.31-21.2, where a tumor suppressor gene cluster resided. Our findings identified a novel locus for NPC and provided a map location fo susceptibility genes candidates. In contrast to a recent study, no significant evidence for NPC linkage to chromosomes 4 and 9 was observed.
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熊炜, 曾朝阳, 李小玲, 李伟芳, 李江贺, 林, 李桂源
ACIA BIOCHIMIA et BIOPHYSICA SINICA 2002, 34 (4): 512-515,-0001,():
-1年11月30日
NGX6基因是本研究室在鼻咽癌9p最小共同缺失区内新克隆的鼻咽癌候选抑瘤基因。通过采用病例-对照研究方法,利用动态等位基因杂交(DASH)技术对105例鼻咽癌患者和183例正常人NGX6基因的2个单核苷酸多态(SNP)进行了分型,经相关分析发现,位于NGX6基因上游调控区的SNP rs879284与鼻咽癌发病存在显著相关性,基因型cT和TT的相对危险度分别为3.93和2.27。实验结果进一步支持了NGX6基因与鼻咽癌的发生发展可能存在密切关系,SNP rs879284由于处在NGX6基因上游调控区域,其多态类型可能在某种程度上影响NGX6基因的表达调控,从而与鼻咽癌发病相关。
single-nuclsotide polymorphisms, NGX6 gene, nasopharyngeal cardnoma, DASH
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【期刊论文】一个新硝基还原酶基因NORl编码区单核苷酸多态及与鼻咽癌的关联分析*
熊炜, 熊炜)**, 曾朝阳)**, 肖炳), 熊芳), 聂新民), 范松青), 彭聪), 李伟芳), 王蓉), 沈守荣), 李小玲), 李桂源)***
生物化学与生物物理进展,2003,30(3):401~405,-0001,():
-1年11月30日
NORl基因是中南大学湘雅医学院肿瘤研究所克隆的一个鼻咽癌表达下调新基因,生物信息学预测NORl基因含有硝基还原酶结构域,该基因可能参与亚硝胺类化学致癌物在体内的代谢过程,从而与鼻咽癌的发生密切相关通过采用病例-对照的研究方法,利用测序技术对144名鼻咽癌患者和匹配的144名正常人NORl基因编码区单核苷酸多态(coding region single nucleotide polymorphisms, csNPs)进行基因分型,关联分析结果显示所检测到的两个csNPs之司存在连锁不平衡,且均与鼻咽癌发病相关,两个csNPs及它们所组成的单倍型相对危险度分别为1.36、1.64和1.37. 两个cSNPs的多态性改变均使NORl基因编码蛋白一级结构发生了变化,这种改变可能影响NORl基因编码蛋白的结构和功能. 研究进一步支持了NORl基因与鼻咽癌的发生发展可能存在密切的关系
单核苷酸多态, NORl基因, 鼻咽癌, 关联分析
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【期刊论文】利用动态等位基因特异性杂交技术进行单核苷酸多态高通量分型*
熊炜, 曾朝阳), 熊炜), 沈守荣), 朱诗国), 李小玲), 李伟芳), 李江), 周鸣), 范松青), 马健), 周洁), 肖炳), 贺林), 李挂源)**
生物化学与生物物理进展,2002,29(5):860~810,-0001,():
-1年11月30日
动态等位基因特异性杂交(dynamic allde-specific hybridization, DASH)是新发展起来的一种单核苷酸(single nucleotide polymorphisms, SNP)等位基因分型技术,具有快速、经济、准确、高通量、重复性好等优点利用DASH技术,对96份正常人外周血DNA样品成功地进行了两个SNP位点的基因分型,并摸索实验条件,对该技术进行了优化
动态等位基因特异性杂交, 单核苷酸多态, 基因分型
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【期刊论文】泛肽相关蛋白基因UBAPl单核苷酸多态及与鼻咽癌的相关研究*
熊炜, 熊炜)**, 曾朝阳)**, 沈守荣), 李小玲), 李伟芳), 周鸣), 李江), 贺林), 冯国鄞), 李挂源)**
生物化学与生物物理进展,2002,29(5):760~770,-0001,():
-1年11月30日
UBAPl基因是在鼻咽癌9p最小共同缺失区内新克隆的鼻咽癌候选抑瘤基因,通过采用病例-对照研究方法,对105例鼻咽癌患者和183例正常人UBAPl基因的5个单核苷酸多态(SNPs)用测序法进行了分型,在实验过程中,偶然发现了一个新的SNP位点,并已登录至dbsNP(登录号: rs3135929)经相关分析发现,位于UBAPl基因第6外显子中的SNP rsl049557与鼻咽癌发病存在显著相关性,基因型GG和GC的相对危险度分别为l.64和l.3l. 实验结果进一步支持了UBAPl基因与鼻咽癌的发生发展可能存在密切关系,SNP rsl049557由于处在UBAPl基因下游3' 非翻译区,其多态类型可能在某种程度上影响UBAP1基因的表达调控,从而与鼻咽癌发病相关
单核苷酸多态, UBAP1基因, 鼻咽癌, 相关分析
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【期刊论文】染色体9p21-22区域两个新的SNPs位点在湖南汉族人群中的多态分布
熊炜, 曾朝阳, 沈守荣, 李小玲, 吕红斌, 向娟娟, 聂新民, 朱诗国, 李伟芳, 王蓉, 贺林, 李桂源
中华医学遗传杂志,2003,20(3):203~206,-0001,():
-1年11月30日
目的在人类染色体9p2l-22区域克隆的两个候选抑瘤基因NGX6和UBAPl以及该区域另一个已知抑瘤基因CDKN2A中筛查未知的单核苷酸多态(single nueleotide polymorphisms, SNPs)。方法用96名正常人外周血白细胞基因组DNA在3个基因的外显子及基因上下游调控区中逐段PCR扩增、大规模测序。结果在UBAPl基因第6外显子及CDKN2A基因第4外显子中各发现一个新的SNP位点, dbSNP登录号为rs3135929和rs3088440,其多态信息含量分别为0.102和0.213。两个SNPs之司不存在连锁不平衡,单倍型分析表明两个SNPs联用,其多态信息含量可达到0.302。结论多个SNP联用,其多态信息含量明显提高,可克服SNPs信息量不足的弱点;两个SNPs均位于基因的3'非翻译区,对基因表达调控是否有影响值得进一步研究。
单核苷酸多态, UBAPl基因, CDKN2A基因
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