周锐
疱疹病毒潜伏感染、动物病原微生物功能基因组学、基因工程疫苗和分子诊断试剂等
个性化签名
- 姓名:周锐
- 目前身份:
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学术头衔:
博士生导师
- 职称:-
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学科领域:
畜牧科学、动物医学
- 研究兴趣:疱疹病毒潜伏感染、动物病原微生物功能基因组学、基因工程疫苗和分子诊断试剂等
周锐,男,1968年6月出生。1996年毕业于华中农业大学,获兽医病理学硕士学位并留校任教,1999-2003年在德国慕尼黑大学基因中心学习和工作,获兽医学博士学位和优秀博士论文奖。现为华中农业大学动物医学院副教授、预防兽医学系副主任。
1996-1999年在华中农业大学动物医学院病毒学与传染病学实验室从事伪狂犬病的研究工作,参加了国家自然科学基金“伪狂犬病基因疫苗研究”和国家生物技术攻关“伪狂犬病基因缺失疫苗研制”等项目的研究。1999-2003年在德国慕尼黑大学基因中心从事胰岛素样生长因子结合蛋白(IGFBP)的功能研究,建立了IGFBP-4和IGFBP-6的转基因小鼠模型,首次通过动物模型阐明了IGFBP-4和IGFBP-6分别对动物免疫系统和消化系统生长发育的负调控作用,同时分析了IGFBP-5对骨肉瘤细胞生长与分化的影响,证实了IGFBP-5的IGF非依赖性作用途径。先后5次参加相关国际学术会议,并作大会或分组报告。2003年10月回国,主要从事疱疹病毒潜伏感染、动物病原微生物功能基因组学、基因工程疫苗和分子诊断试剂等方面的研究工作,正在主持国家自然科学基金、国家“十五”科技攻关、农业部“948”项目、留学回国基金、中国博士后科学基金、湖北省自然科学基金等项目的研究。先后主讲《病毒学》、《动物流行病学》和《兽医学》等课程,目前指导在读硕士研究生9名,协助指导博士研究生2名。在Journal of Molecular Endocrinology, Journal of Endocrinology, Biochemical and Biophysical Research Communications, Vaccine, 畜牧兽医学报,生物工程学报,农业生物技术学报等学术期刊上发表论文20余篇。
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【期刊论文】猪伪狂犬病油乳剂灭活疫苗的制备及安全性与免疫性试验
周锐, 陈焕春, 金梅林, 何启盖, 方六荣, 吴美洲, 周斌
畜牧兽医学报,2001,32(1),44~51,-0001,():
-1年11月30日
用本室分离鉴定的猪伪狂犬病毒鄂A 强毒株接种仓鼠肾细胞(BHK221),制备病毒抗原液,毒价不低于10-6 TCID50/011ml。经一定浓度的甲醛溶液灭活后与油相佐剂乳化研制成油乳剂灭活油疫苗4批。本研究对该制品的安全性、免疫性进行了测定。对18g左右小白鼠接种013ml,初生仔猪、断奶仔猪及妊娠母猪加倍剂量注射,均未出现不良反应,安全性良好。对母猪的繁殖性能不产生影响。后备母猪及妊娠母猪血清中和抗体指数于免疫后21d 达到316 以上,间隔35d强免疫一次后,中和抗体指数可达1000以上。断奶仔猪及初生仔猪免疫后对强毒的攻击,保护率分别为100%及90.62%。
猪伪狂犬病, 灭活疫苗, 微量中和试验, 安全性, 免疫性
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【期刊论文】伪狂犬病病毒鄂A株gG-/LacZ+突变株的构建
周锐, 周复春, 陈焕春, 方六荣, 吴斌, 何启盖
畜牧兽医学报,2001,32(2),129~133,-0001,():
-1年11月30日
以从湖北某猪场分离鉴定的鄂A株为亲本,提取其基因组DNA,克隆含gG基因的SphⅠ/KpnⅠ片段,然后将LacZ基因融合到gG启动子下游,得到重组质粒pUSKZ,将重组质粒与鄂A株基因组共转染PK215细胞,待细胞完全病变后,在X2gal存在下,作蓝斑筛选纯化。经斑点杂交和PCR扩增证实得到的是基因型为gG-/LacZ+的重组伪狂犬病病毒。
伪狂犬病病毒, 鄂A株, gG-/, LacZ+, 突变株
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周锐, 洪文洲, 陈焕春, 方六荣, 何启盖, 吴斌
畜牧兽医学报,2001,32(3),235~243,-0001,():
-1年11月30日
本研究从含有伪狂犬病病毒(Pseudorabies virus,PRV)Ea株gD基因BamHI 616Kb片段的重组质粒pUCB7中分别亚克隆gD基因的上、下游片段,并对上游片段进一步改造,去掉gD基因上游的非编码序列,构建了含完整gD基因编码区的重组质粒pBRgDI,并利用基因内部的限制性内切酶位点,用内切酶KpnI和SalI酶切分析,证实了gD基因的可靠性和完整性。同时构建了测序质粒pSKgDSB和pSKgDS,并用双脱氧终止法进行测序。将测序结果与国外的Rice毒株进行比较,发现Ea株gD基因核苷酸序列有多处点突变和一处插入突变,在编码氨基酸残基水平上也有差异。
伪狂犬病病毒, Ea株, gD基因, 克隆, 序列分析
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【期刊论文】IGF-binding protein-4 -biochemical characteristics and functional consequences
周锐
,-0001,():
-1年11月30日
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周锐, Wenzhou Hong, Shaobo Xiao, Rui Zhou, Liurong Fang, Qigai He, Bin Wu, Fuchun Zhou, Huanchun Chen∗
Vaccine 20(2002)1205-1214,-0001,():
-1年11月30日
Glycoprotein gene gB, gC and gD of pseudorabies virus (PrV) strain Ea, which was isolated locally in Wuhan, were cloned from the viral genome DNA and expressed in vitro controlled by the major immediately-early promotor/enhancer of HCMV. In the presented paper, Balb/c mice, rabbits and piglets were vaccinated intramuscularly two times at 2-week interval wth those eukaryotic expression plasmid pcDB, pcDC and pcDD, respectively. The animals injected with pcDB, pcDC, pcDD or mix DNA developed anti-PrV antibodies. Neutralizing antibody titers obtained 2-5 log2, 2 weeks after the second vaccination. Cellular immune responses were also detected by lymphoproliferation assay and cytotoxic T lymphocyte (CTL) activity assay in all groups vaccinated with DNA. Immune responses elicited by DNA vaccines provided protections with different degrees against lethal dose PrV challenge. In mice, protections induced by pcDC, pcDD or mix DNA were 100%, similar to that by inactivated vaccine. Protections were more than 50% induced by pcDC, pcDD or mix DNA in rabbits. Protections induced by pcDB were the lowest among DNA immunization in mice or rabbits. However, pcDB could elicit the higher cellular responses in rabbits or piglets. In piglets, body temperatures of animals injected with pcDB, pcDC, pcDD or mix DNA did not change significantly after challenge with 2
Pseudorabies virus, Glycoprotein genes, DNA vaccines, Neutralizing antibody, CTL, Protection
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【期刊论文】Insulin-like growth factor-binding protein-4 inhibits growth of the thymus in transgenic mice
周锐, R Zhou, , H Flaswinkel, M R Schneider, H Lahm, A Hoeflich, R Wanke and E Wolf
Journal of Molecular Endocrinology (2004) 32, 349-364,-0001,():
-1年11月30日
Numerous in vitro studies have demonstrated that IGF-binding protein (IGFBP)-4 is a consistent inhibitor of IGF actions. In order to investigate the functions of IGFBP-4 in vivo, transgenic mice were generated by microinjection of a transgene, in which the murine Igfbp4 cDNA is driven by the H-2Kb promoter, and followed by a splicing cassette and polyadenylation signal of the human-globin gene. Transgene mRNA was expressed ubiquitously, and elevated IGFBP-4 protein was detected in the spleen, thymus, kidney and lung of transgenic mice. The activities of serum IGFBPs were not changed in transgenic mice. Immunohistochemical studies revealed transgene expression predominantly in the thymic medulla and red pulp of the spleen. Body weight and the weights of the spleen, kidney and lung of transgenic mice were not different from controls. In contrast, the thymus of transgenic mice showed a significantly reduced weight and cortex volume. In transgenic thymus and spleen, cell proliferation was inhibited and apoptosis was stimulated. Transgenic mice showed normal T-and B-cell development and normal basal plasma immunoglobulin levels. In conclusion, overexpression of IGFBP-4 inhibits growth of the thymus. IGFBP-4 excess inhibits cell proliferation and stimulates apoptosis in lymphoid tissues, but does not affect lymphocyte development. These findings suggest that IGFBP-4 is a potential growth inhibitor of lymphoid tissues.
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周锐, Weicheng Bei, Qigai He, Lin Yan, Liurong Fang, Yadi Tan, Shaobo Xiao, Rui Zhou, Meilin Jin, Aizhen Guo, Jianqiang Lv, Hongliang Huang, Huanchun Chen *
FEMS Microbiology Letters 243(2005)21-27,-0001,():
-1年11月30日
The apxIIC gene of Actinobacillus pleuropneumoniae serotype 7 was inactivated by homologous recombination using a sucrose counter-selectable marker system, resulting in a mutant strain that had no antibiotic resistance marker and expressed an inactivated ApxII toxin. The safety and immunogenicity of the mutant were evaluated in mice. The mutant strain caused no adverse effects in mice at doses up to 2
Actinobacillus pleuropneumoniae, apxIICA, Sucrose counter selection marker, Vaccine
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周锐, Marlon R. Schneider, *, Rui Zhou, * Andreas Hoeflich, * Ottheinz Krebs, * Jorg Schmidt, † Subburaman Mohan, ‡ Eckhard Wolf, * and Harald Lahm*
Biochemical and Biophysical Research Communications 288, 435-442 (2001),-0001,():
-1年11月30日
The precise role of insulin-like growth factor-binding protein-5 (IGFBP-5) in regulating the growth of tumor cells, especially of bone-derived malignant cells, is not well understood. We have investigated the biological activity of IGFBP-5 by transfecting OS/50-K8 mouse osteosarcoma cells with an expression vector containing the osteocalcin promoter and the complete mouse IGFBP-5 cDNA (OC-IGFBP-5). Overexpression of IGFBP-5 mRNA and secretion of increased amounts of bioactive protein in conditioned media were demonstrated in different clones. For the analysis of cell proliferation, three clones exhibiting high levels of IGFBP-5 expression were selected and compared to a mock clone and to nontransfected parental cells. IGFBP-5-secreting clones displayed reduced proliferation under both anchorage-dependent and -independent conditions (P<0.05). The increase in proliferation observed in IGFBP-5-secreting clones after addition of exogenous IGF was significantly lower than that observed in mock-transfected or parental cells. A similar result was obtained with long[R3]IGF-I which has a low affinity for all IGFBPs, suggesting that the inhibitory effect of IGFBP-5 is only partially IGF-dependent. OC-IGFBP-5-transfected clones expressed significantly higher amounts of osteocalcin mRNA (P<0.05) and secreted more osteocalcin protein than a mock clone or parental OS-50/K8 cells. Thus, part of the growthinhibiting effect of IGFBP-5 may be due to an induction of differentiation in these cells.
differentiation, IGF, IGFBP-5, OS/, 50-K8, osteocalcin, osteosarcoma, overexpression, transfection.,
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