孙宝林
厌氧脱氯微生物的生理、生态和遗传。脱氯呼吸的生化和遗传机理。环境基因组学。
个性化签名
- 姓名:孙宝林
- 目前身份:
- 担任导师情况:
- 学位:
-
学术头衔:
博士生导师
- 职称:-
-
学科领域:
微生物学
- 研究兴趣:厌氧脱氯微生物的生理、生态和遗传。脱氯呼吸的生化和遗传机理。环境基因组学。
孙宝林,教授,博士生导师。中科院“百人计划”引进人才。中国科学技术大学生命科学学院环境微生物学实验室主任。研究方向:厌氧脱氯微生物的生理、生态和遗传。脱氯呼吸的生化和遗传机理。环境基因组学。学习及工作简历:12/2003-至今:教授,中国科学技术大学生命科学学院。研究方向为环境微生物学和环境基因组学。01/2003-10/2003:研究助理教授,Michigan State University微生物生态中心。研究方向为厌氧还原脱氯微生物的生态、生理、分类和遗传学;利用分子生物学方法检测微生物群体;还原脱氯酶的克隆、分析及鉴定。08/2000-09/2000:访问博士后,Stanford University微生物系。研究方向为还原脱氯酶的分子克隆及其在饥饿条件下的表达。11/1999-12/2002:博士后,Michigan State University微生物生态中心。研究方向为厌氧脱氯微生物的生态、生理和遗传学;三氯乙烷脱氯细菌的富集、分离及还原脱氯酶的分子克隆;利用T-RFLP、ARDRA和16S核糖体DNA序列分析对四氯乙烯、三氯乙烯及RDX降解细菌的分子鉴定及跟踪。01/1995-08/1999:博士研究生,Michigan State University作物及土壤系及微生物生态中心。博士论文项目:海洋还原脱氯微生物的富集、分离及鉴定。
-
主页访问
3007
-
关注数
0
-
成果阅读
192
-
成果数
3
【期刊论文】Microbial Dehalorespiration with 1,1,1-Trichloroethane
孙宝林 , Baolin Sun, , Benjamin M. Griffin, Hector L. Ayala-del-Rio, Syed A. Hashsham, James M. Tiedje, *
,-0001,():
-1年11月30日
1,1,1-Trichloroethane (TCA) is a ubiquitous nvironmental pollutant because of its widespread use as an industrial solvent, its improper disposal, and its substantial emission to the atmosphere. We report the isolation of an anaerobic bacterium, strain TCA1, that reductively dechlorinates TCA to 1, 1-dichloroethane and chloroethane. Strain TCA1 required H2 as an electron donor and TCA as an electron acceptor for growth, indicating that dechlorination is a respiratory process. Phylogenetic analysis indicated that strain TCA1 is related to gram-positive bacteria with low DNA G C content and that its closest relative is Dehalobacter restrictus, an obligate H2-oxidizing, chloroethene-respiring bacterium.
-
104浏览
-
0点赞
-
0收藏
-
0分享
-
263下载
-
0评论
-
引用
孙宝林 , BAOLIN SUN, , JAMES R. COLE, ROBERT A. SANFORD, AND JAMES M. TIEDJE*
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, June 2000, p. 2408-2413,-0001,():
-1年11月30日
Strain SF3, a gram-negative, anaerobic, motile, short curved rod that grows by coupling the reductive dechlorination of 2-chlorophenol (2-CP) to the oxidation of acetate, was isolated from San Francisco Bay sediment. Strain SF3 grew at concentrations of NaCl ranging from 0.16 to 2.5%, but concentrations of KCl above 0.32% inhibited growth. The isolate used acetate, fumarate, lactate, propionate, pyruvate, alanine, and ethanol as electron donors for growth coupled to reductive dechlorination. Among the halogenated aromatic compounds tested, only the ortho position of chlorophenols was reductively dechlorinated, and additional chlorines at other positions blocked ortho dechlorination. Sulfate, sulfite, thiosulfate, and nitrate were also used as electron acceptors for growth. The optimal temperature for growth was 30℃, and no growth or dechlorination activity was observed at 37℃. Growth by reductive dechlorination was revealed by a growth yield of about 1g of protein per mol of 2-CP dechlorinated, and about 2.7g of protein per mole of 2,6-dichlorophenol dechlorinated. The physiological features and 16S ribosomal DNA sequence suggest that the organism is a novel species of the genus Desulfovibrio and which we have designated Desulfovibrio dechloracetivorans. The unusual physiological feature of this strain is that it uses acetate as an electron donor and carbon source for growth with 2-CP but not with sulfate.
-
57浏览
-
0点赞
-
0收藏
-
0分享
-
264下载
-
0评论
-
引用
【期刊论文】Desulfomonile limimaris sp. nov., an anaerobic dehalogenating bacterium from marine sediments
孙宝林 , Baolin Sun, James R. Cole and James M. Tiedje
International Journal of Systematic and Evolutionary Microbiology (2001), 51, 365-371,-0001,():
-1年11月30日
Strains DCB-MT and DCB-F were isolated from anaerobic 3-chlorobenzoate (3CB)-mineralizing cultures enriched from marine sediments. The isolates are large, Gram-negative rods with a collar girdling each cell. The isolates are obligate anaerobes capable of reductive dechlorination of 3CB to benzoate. Growth by chlororespiration in strain DCB-MT yielded 1<7g protein molN1 3CB dechlorinated with lactate as the electron donor. Strain DCB-MT also used fumarate, sulfate, sulfite, thiosulfate and nitrate as physiological electron acceptors for growth, but grew poorly on sulfate and nitrate. Reductive dechlorination was inhibited completely by sulfite and thiosulfate but not by sulfate. Both strains were incapable of growth at NaCl concentrations below 0<32% (w/v). They grew well at sea-water salt concentrations; however, the optimum growth rate was achieved at a NaCl concentration half that of sea water. The 16S rDNA sequence analysis shows strains DCB-MT and DCB-F to be 99% similar to each other and 93% similar to their closest relative, Desulfomonile tiedjei strain DCB-1T. Strain DCB-MT can also be distinguished from strain DCB-1T by its inability to use acetate for growth on 3CB and by its requirement for NaCl. The morphology, physiology and 16S rDNA sequences of DCB-MT and DCB-F suggest that these strains represent a new, marine-adapted species of the genus Desulfomonile, designated Desulfomonile limimaris sp. nov. The type strain is strain DCB-MT (=ATCC 700979T).
Desulfomonile limimaris,, reductive dehalogenation,, marine sediments
-
31浏览
-
0点赞
-
0收藏
-
0分享
-
132下载
-
0评论
-
引用