双少敏
1 超分子化学;2 化学与生物传感器。
个性化签名
- 姓名:双少敏
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学术头衔:
博士生导师
- 职称:-
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学科领域:
分析化学
- 研究兴趣: 1 超分子化学;2 化学与生物传感器。
双少敏,教授,博士生导师。 1986年毕业于山西大学化学系,1989年山西大学硕士毕业。后留校任教。1995年攻读华南理工大学化学与生物工程学院博士。1998年获博士学位,1999赴香港浸会大学化学系作博士后研究两年。2004年10月赴加拿大多伦多大学化学系做高访。现为化学化工学院化学系主任,山西大学现代化学研究所超分子化学研究室主任,教学骨干和山西省高等院校青年学科带头人。兼任亚太环糊精化学委员会成员,《分析化学》编委,山西大学九三学社委员会副主任委员,山西省政协委员。研究方向 1 超分子化学2 化学与生物传感器。在国内外核心期刊如“J.Org. Chem.”, “Anal.Chim.Acta.”, “Sensor Actuators:B Chemical” “Talanta”,《化学学报》,《分析化学》等上发表研究论文80余篇, 被SCI收录40余篇。多次参加国内、国际学术会议,并被国际核心刊物Anal.Chim.Acta., Talata., J.Chrogr.B ,J.Photochem.Photobio.多次指定为审稿人。现指导博士、硕士研究生12人,并与香港浸会大学联合培养硕士和博士研究生,已陆续派出博士,硕士研究生进行合作研究。获奖情况:1)2004年获得国家技术发明二等奖(名次第三)2)2004年获得山西省科技进步理论一等奖(名次第一)3)2002年获得山西大学“富士康”优秀教师奖。所授课程被评为学校“优师优课”4)2001年获山西省科技进步理论二等奖,成果名称:环糊精超分子研究。5)2000年获山西省科技进步理论二等奖。6)1998年获国家教委科技进步三等奖。先后主持国家自然科学基金一项,国家教育部优秀青年教师研究基金一项,山西省自然科学基金一项。山西省归国留学基金一项。
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812
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成果数
13
【期刊论文】Effect of microscopic environment on the self-stacking binding of porphyrin to DNA
双少敏, An Wenting, Guo Xiliang, Shuang Shaomin*, Dong Chuan
Journal of Photochemistry and Photobiology A: Chemistry 173(2005)36-41,-0001,():
-1年11月30日
thymus DNA (ctDNA) was assumed by the change of UV-vis spectroscopy, fluorescence and resonance light-scattering (RLS). The changed absorption spectral properties of TEPyP titrated with ctDNA were observed from red shifted (λ=14nm) and a large hypochromicity (42%). In fluorescence spectra, λex,max of TEPyP shifted to longer wavelength (λ=13nm) and a decrease around 50% of the emission intensity were shown. The signal of resonance light-scattering was enhanced by nucleic acid. The study on the inclusion interaction of TEPyP with β-cyclodextrin (β-CD) and its derivatives including hydroxypropyl- -cyclodextrin (HP- -CD), sulfobutylether-β-cyclodextrin (SBE-β-CD) shown that the formation constants for β-CD, HP-β-CD and SBE-β-CD with TEPyP were 550, 1.1×104 and 2.0×105M−1, respectively, with 1:1 stoichiometry. The conformation confirmed by 1HNMR technique is that the alkyl and pyrrole cyclic moiety entered into the cavity of cyclodextrins. Comparative study on the interaction of TEPyP with ctDNA was further carried out in the presence of β-CD, HP-β-CD, and SBE- -CD. The significant decrease of the binding constants and binding numbers were observed and the interaction of DNA-bound porphyrin has been inhibited. Moreover, the stronger inclusion ability of TEPyP and CDs, the bigger influence on the interaction of TEPyP with DNA, which was due to the fact that TEPyP enter into the cavity of CDs. It may influence binding affinity of porphyrin to DNA and weaken the static electronic forces in the outside self-stacking. Typically, the negative SBE-β-CD hampers directly the binding of the negative phosphate groups of the DNA backbone and the positive TEPyP.
Meso-tetrakis(, 4-N-ethylpyridiumyl), porphyrin, Calf thymus DNA (, ctDNA), , Cyclodextrin, Fluorescence, Resonance light-scattering
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双少敏, Guomei Zhang, Shaomin Shuang*, Chuan Dong, Jinghao Pan
Spectrochimica Acta Part A 59(2003)2935-2941,-0001,():
-1年11月30日
The ability of b-cyclodextrin (b-CD), hydroxypropyl-b-cyclodextrin (HP-b-CD), and carboxymethyl-b-cyclodextrin (CM-b-CD) to break the aggregate of the methylene blue (MB) and to form 1:1 inclusion complexes has been studied by absorption and fluorescence spectroscopy. Experimental conditions including concentrations of various cyclodextrins (b-CD, HP-b-CD and CM-b-CD) and media acidity were investigated for the inclusion formation in detail. The formation constants are calculated by using steady-state fluorimetry, from which the inclusion capacity of different cyclodextrins (CDs) is compared. The results suggest that the charged b-cyclodextrin (CM-b-CD) is more suitable for inclusion of the cationic dye MB than the neutral b-cyclodextrins (b-CD, HP-b-CD) at pH/5. A mechanism is proposed which is consistent with the stronger binding of MB with CM-b-CD compared with the other CDs at pH/5.
Cyclodextrin, Methylene blue, Fluorescence
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双少敏, Martin M.F. Choi*, Shaomin Shuang, H.Y. Lai, S.C. Cheng, Rebecca C.W. Cheng, Bohr K.B. Cheung, Albert W.M. Lee*
Analytica Chimica Acta 516(2004)155-163,-0001,():
-1年11月30日
A gas chromatography-mass spectrometric (GC-MS) method for the determination of total isothiocyanates (ITCs) has been developed based on the quantification of the cyclocondensation product, 1,3-benzodithiole-2-thione, between ITCs and 1,2-benzenedithiol. It was the first analytical application to be applied to the analysis of Chinese medicinal herbs including Laifuzi, Baijiezi, Tinglizi and Hancai, and seeds of commonly consumed vegetables.Aconsiderable variation in ITC contentswas observed. The highest concentrations of total ITCs were found to be 20.3mol/g in Laifuzi and 12.6 mol/g in seeds of Chinese kale. The method demonstrates the usefulness of the cyclocondensation of ITCs with 1,2-benzenedithiol reagent for quantifying total ITC contents in Chinese medicinal herbs regardless of the types of ITC. The linear dynamic range covered from 0.12 to 200 MITC. The limit of detection (3Sb)was 35nMand the corresponding absolute detection limitwas 35 fmol. The proposedGC-MStechnique provides a reliable, unambiguous, reproducible, and efficient method for determining total ITCs of various samples.
Isothiocyanates, Chinese medicinal herbs, Gas chromatography-mass spectrometry
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【期刊论文】Study on vitamin K3-cyclodextrin inclusion complex and analytical application
双少敏, Dong Zhenming, Liu Xiuping, Zhang Guomei, Shuang Shaomin*, Pan Jinghao
Spectrochimica Acta Part A 59(2003)2073-2079,-0001,():
-1年11月30日
The inclusion interaction of the complexes between Vitamin K3 (VK3) and b-cyclodextrin (b-CD), hydroxypropyl-bcyclodextrin (HP-b-CD) and sulfobutylether-b-cyclodextrin (SBE-b-CD) were studied by using steady-state fluorescence measurements. The various factors affecting the inclusion process were examined in detail. The formation constants and inclusion stoichiometry for VK3/CDs were determined. The results showed that the inclusion ability of b-CD and its derivatives was the order: SBE-b-CD/HP-b-CD /b-CD. The related inclusion mechanism is proposed to explain the inclusion process. A method of determining VK3 was established with the linear range was 2.5/10 6/5.0/10 4M, and was used to determine the VK3 tablets. The recoveries were in the range of 97.52/103.5%. The results were satisfactory.
Cyclodextrin, Vitamin K3, Fluorescence
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【期刊论文】Investigation on DNA assembly to neutral red-cyclodextrin complex by molecular spectroscopy
双少敏, Guomei Zhang a, Shaomin Shuang a, *, Chuan Dong a, Diansheng Liu a, Martin M.F. Choi b
Journal of Photochemistry and Photobiology B: Biology 74(2004)127-134,-0001,():
-1年11月30日
DNA assembling to neutral red (NR) and cyclodextrins (CDs)-NR inclusion complex has been investigated by means of absorption, fluorescence and resonance light scattering (RLS). Depending on the molar ratio R of NR:DNA, the binding of NR with DNA involved in two processes at pH 7.50 and ionic strength 0.0045. The first process occurs in R > 2:5, where the neutral form of R was predominant and enhanced RLS was observed, indicating the aggregation of NR neutral form molecules on the molecular surface of DNA. The second process occurs in R < 2:5, where an absorption band at 540nm and a fluorescent excitation and emission band at 550 and 607 nm respectively provided compelling evidence that the binding of NR to DNA leaded to extensive NR protonation even at pH 7.50, and that a protonated NR (the acidic form of NR) can form DNA adducts with a binding mode different from that of the unprotonated form (the neutral form of NR). The results were also illustrated by the CDs-NR supramolecular system. The experimental data showed that CDs including b-CD, hydroxypropyl-b-CD (HP-b-CD) and sulfobutyletherb-CD (SBE-b-CD) superior to include the neutral form of NR, in addition, the inclusion complex decomposed when it bound to DNA. Thus, the decomposed NR was also protonated to form DNA adducts with intercalative mode. In fact, CDs played a role to carry guest molecule to intercalate DNA. A related mechanism is proposed.
Neutral red, DNA, Supramolecular system, Cyclodextrin
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双少敏, Baoli Wu a, Guomei Zhang a, Shaomin Shuang a, *, Martin M.F. Choi b,
Talanta 64(2004)546-553,-0001,():
-1年11月30日
he oxygen level was monitored and related to the glucose concentration. The effect of glutaraldehyde concentration, pH, phosphate buffer concentration and temperature on the response of the glucose biosensor has been studied in detail. Common matrix interferents such as ethanol, d-fructose, citric acid, sodium benzoate, sucrose and l-ascorbic acid did not give significant interference. The resulting sensor exhibited a fast response (100s), high sensitivity (8.3409mg L−1 oxygen depletion/mmol L−1 glucose) and good storage stability (85.2% of its initial sensitivity after 4 months). The linear response is 1.0×10−5 to 1.3×10−3mol L−1 glucose. The glucose content in real samples such as commercial glucose injection preparations and wines was determined, and the results were comparable to the values obtained from a commercial glucose assay kit based on a spectrophotometric method.
Glucose biosensor, Eggshell membrane, Glucose oxidase
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【期刊论文】Preparation and spectral investigation on inclusion complex of β-cyclodextrin with rutin
双少敏, Ding Haiyun, Chao Jianbin, Zhang Guomei, Shuang Shaomin *, Pan Jinhao
Spectrochimica Acta Part A 59(2003)3421-3429,-0001,():
-1年11月30日
Solid inclusion complex of rutin with b-cyclodextrin (b-CD) was prepared by coprecipitate method. The formation of inclusion complex was confirmed by differential scanning calorimetry (DSC) and X-ray diffraction. The formation constant was obtained by steady-state fluorescence measurements and the result suggested the complex preferred 1:1 (rutin:CD) stoichiometry. Furthermore, the spatial configuration of the complex has been proposed based on NMR and molecular modeling.
β-Cyclodextrin, Rutin, Fluorescence, Nuclear magnetic resonance
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双少敏, Yue Hong Pang a, Li Li Yang a, Shao Min Shuang a, *, Chuan Dong a, Michael Thompson b
Journal of Photochemistry and Photobiology B: Biology 80(2005)139-144,-0001,():
-1年11月30日
The interactions between bendroflumethiazide (BFTZ) and human serum albumin (HSA) have been studied by fluorescence spectroscopy. Binding constants for drug attachment to the various binding sites of HSA have been measured at different temperatures in physiological buffer solution. The effect of metal ions on BFTZ interaction with HSA was also investigated. The thermodynamic parameters, DH and DS, have been calculated to be 49.28kJmol 1>0, and 258.83Jmol 1K 1>0, respectively. The distance between HSA and BFTZ, r, was determined to be 1.47nm based on Forster s non-radiative energy transfer theory. The experimental results reveal that BFTZ has a strong ability to quench the intrinsic fluorescence of HSA through a static quenching mechanism. Furthermore, the binding constants between BFTZ and HSA are remarkably independent of temperature, and decrease in the presence of various ions, usually by about 30-55%. Hydrophobic interaction occurs between BFTZ and the sub-domain II A of HSA.
Human serum albumin, Bendroflumethiazide, Steady-state fluorescence
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双少敏, Guomei Zhang, Shaomin Shuang*, Zhenming Dong, Chuan Dong, Jinghao Pan
Analytica Chimica Acta 474(2002)189-195,-0001,():
-1年11月30日
The formation of the inclusion complexes of neutral red (NR) with cyclodextrins (CDs) including -βcyclodextrin (β-CD), hydroxypropyl-β-cyclodextrin (HP-β-CD) and sulfobutylether-β-cyclodextrin (SBE-β-CD) was studied by fluorescence and UV-Vis absorption spectroscopy and the binding constants (K) of the inclusion complexes were obtained by steady-state fluorescence measurements. Experimental conditions including the concentrations of various CDs and media acidity were investigated in detail. The results suggested that NR exists in two molecular forms in aqueous solu ion (the acidic form and the neutral form). CDs were most suitable for inclusion of the neutral form of NR and could cause enhanced fluorescence emission and absorption by NR. Moreover, the effect of the charged CD (SBE-β-CD) was quite different from that of the uncharged CDs (β-CD and HP-β-CD). A mechanism is proposed to explain the inclusion process.
Cyclodextrins, Neutral red, Fluorescence, UV-Vis absorption
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双少敏, Yue Hong Pang a, Shao Min Shuang a, *, Man Shing Wong b, , Zhong Hui Li b, Chuan Dong a
Journal of Photochemistry and Photobiology A: Chemistry 170(2005)15-19,-0001,():
-1年11月30日
A new compound 4-(diphenylamino) biphenyl-4 -boronic acid (DBBA) was synthesized. The optical properties including fluorescence quantum yields and change in the dipole moment in various solvents at room temperature were characterized by the absorption and steadystate fluorescence technique. The emission wavelength of DBBA was very sensitive to the polarity of solvents. The magnitude of change in the dipole moment was calculated based on the Lippert-Mataga equation. DBBA has an increase of dipole moment of 4.9 D units on excitation to the lowest singlet state. A high fluorescence quantum yields (φf) and short fluorescence decay times (τf) were determined in all solvents tested. The bathochromic shift of the emission spectra and the increase in the excited state dipole moment occurred. These gave the evidence about the intramolecular charge transfer (ICT) character in the emitting singlet state of DBBA.
Intramolecular charge transfer, Fluorescence, Dipole moment
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