钟建江
生物化学工程、植物细胞培养、发酵工程、天然产物生物工程、生物环境工程等
个性化签名
- 姓名:钟建江
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学术头衔:
博士生导师, 国家“百千万”人才工程国家级人选 国家杰出青年科学基金获得者,
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学科领域:
生物化学
- 研究兴趣:生物化学工程、植物细胞培养、发酵工程、天然产物生物工程、生物环境工程等
1965年4月生,93年3月获大阪大学应用生物学博士学位。96年12月至今任华东理工大学教授。现为博士生导师,生物反应器工程国家重点实验室主任,长江计划特聘教授。兼任国际生物化工知名刊物Biotechnology and Bioengineering、Enzyme and Microbial Technology、Journal of Bioscience and Bioengineering、Process Biochemistry、Biochemical Engineering Journal、Advances in Biochemiscal Engeneering/Biotechnology 等编委或顾问编委,生物反应器工程国家重点实验室学术委委员,教育部工业生物技术重点实验室学术委委员;(曾)任多个国际会议的大会主席和组织委委员。 主要从事生物化学工程、植物细胞培养、发酵工程、天然产物生物工程、生物环境工程等。主持国家自然科学基金及重点项目、国家杰出青年科学基金等课题。 典型成果有:提出新型细胞培养反应器,开拓了通过监测SOUR来控制补料时机并实现高密度细胞培养高校生产人参皂甙和多糖的方法,提出了两阶段培养高效生产药理活性物质灵芝酸的方法,发现了植物细胞培养液平汉塑性流动现象,定量研究了剪切、混合、供氧和光照等对细胞培养过程影响。发表SCI收录论文80余篇、被他人引用200片次,获国家发明专利证书5项,由Springer-Verlag公司出版学术著作2本,在境外召开的国际会议上做大会和特邀报告10余次。曾获"全国优秀留学回国人员"誉,2002年被日本生物工程学会授予首个国际学术奖Young Asian Biotechnologist Prize。
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钟建江
,-0001,():
-1年11月30日
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钟建江, Zhi-Wei Pan†, Hong-Qiang Wang†, Jian-Jiang Zhong*
Enzyme and Microbial Technology 27(2000)714-723,-0001,():
-1年11月30日
Suspension cells of Taxus chinensis were cultivated in both shake flasks and bioreactors. The production of taxuyunnanine C (TC) was greatly reduced when the cell cultures were transferred from shake flasks to bioreactors. Oxygen supply, shear stress and stripping-off of gaseous metabolites were considered as potential factors affecting the taxane accumulation in bioreactors. The effects of oxygen supply on the cell growth and metabolism were investigated in a stirred tank bioreactor by altering its oxygen transfer rate (OTR). It was found that both the pattern and amount of TC accumulation were not much changed within the range of OTR as investigated. Comparative studies on the cell cultivation in low shear and high shear generating bioreactors suggest that the decrease of TC formation in bioreactors was not due to the different shear environments in different cultivation vessels. An incorporation of 2% CO2 in the inlet air was beneficial for the cell growth, but did not improve the TC production in bioreactors. Furthermore, the effects of different levels of ethylene addition into the inlet air on the cell growth and TC production were investigated in a bubble column reactor. The average cell growth rate increased from 0.146 to 0.204 d21 as the ethylene concentration was raised from 0 to 50 ppm, and both the content and production of TC were also greatly improved by ethylene addition. At an ethylene concentration of 18 ppm, the highest TC content and volumetric production in the reactor reached 13.28mg/(g DW) and 163.7mg/L, respectively, which were almost the same as those in shake flasks. Compared with the control reactor (bubble column without ethylene supplementation), the maximum TC content was increased by 82% and the total production of TC was doubled. The results indicate that ethylene is a key factor in scaling up the process of the uspension cultures of T. chinensis from a shake flask to a bioreactor.
Taxus chinensis, Taxane production, Plant cell suspension culture, Scale-up study, Ethylene effect
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钟建江, Hao-Di Dong, Jian-Jiang Zhong∗
H.-D. Dong, J.-J. Zhong/Enzyme and Microbial Technology 31(2002)116-121,-0001,():
-1年11月30日
In conventional suspension cultures of Taxus chinensis, the accumulation of physiologically active taxuyunnanine C (Tc) in 1l (working volume) airlift bioreactors was lower than in shake flasks. Elicitation by methyl jasmonate (MJA) or sucrose feeding could improve the taxane production in the bioreactors.Astrategy of combining MJAelicitation and sucrose feedingwas applied to cell cultures in bioreactors. Both the Tc production and productivity were significantly enhanced by combined addition of 100 M MJA and 20gl−1 sucrose at day 7 and their maximum levels reached were 344±12mgl−1 (day 18) and 18.6±0.5mgl−1 day−1 (until day 15), respectively. A further combination of MJA elicitation (at 100 M), sucrose feeding (at 20gl−1) and ethylene incorporation (at 18 ppm) in the inlet air improved the Tc productivity to 20.2±0.4mgl−1day−1 in the bioreactors. The results suggest that the above combination approach is a useful strategy for large-scale production of taxane diterpenes by plant cell cultures.
Taxus chinensis, Taxoid production, Bioreactor engineering, Plant cell culture, Combination strategy, Secondary metabolite
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钟建江, Zhan-Ying Zhang and Jian-Jiang Zhong*
,-0001,():
-1年11月30日
Scale-up of a novel centrifugal impeller bioreactor (CIB) was demonstrated for production of valuable plant-specific secondary metabolites by high-density cell cultures. Initial kLa was identified to be a key factor affecting cell growth and production of ginseng saponin and polysaccharide by high-density cultivation of Panax notoginseng cells in a 3-L CIB. A high level of ginseng saponin and polysaccharide production was obtained at an initial kLa value of 30.2 h-1. A maximum dry cell weight (DW) and production titer of ginseng saponin and polysaccharide reached 22.0 (0.3, 1.5
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钟建江, Ya-Jie Tang and Jian-Jiang Zhong*
Biotechnol. Prog. 2003, 19, 1842-1846,-0001,():
-1年11月30日
Scale-up of a liquid static culture process was studied for hyperproduction of ganoderic acid (GA) by a famous Chinese traditional medicinal mushroom, Ganoderma lucidum. Initial volumetric oxygen transfer coefficient (KLa) and area of liquid surface per liquid volume (As) were identified as key factors affecting cell growth and GA accumulation in liquid static cultures of G. lucidum, on the basis of which a multilayer static bioreactor was designed. At a low initial KLa level of 2.1 h-1, a thick layer of white mycelia was formed on the liquid surface, and an optimal production of total GA (i.e., GA production in the liquid and on the liquid surface) was obtained. Both the formation of white mycelia and production of GA on the liquid surface were enhanced with an increase of As within the range as investigated (0.24-1.53cm2/mL). At an As value of 0.90cm2/mL, the total GA production reached maximum. A successful scale-up from a 20-mL static T-flask to a 7.5-L three-layer static bioreactor was achieved based on initial KLa. The maximum biomass (20.8
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【期刊论文】Novel Fermentation Strategy for Enhancing Glycerol Production by Candida krusei
钟建江, Hong-Juan Liu, †, ‡ De-Hua Liu, *, † and Jian-Jiang Zhong*, ‡
Biotechnol. Prog. 2003, 19, 1615-1619,-0001,():
-1年11月30日
During the later stage of glycerol production by fermentation of Candida krusei, glycerol consumption by the strain was observed, although there was residual sugar in the medium. To enhance the final glycerol accumulation, a new fermentation strategy was developed by maintaining high activities of glycerol synthetic enzymes (i.e., glycerol-3-phosphate dehydrogenase (ctGPD) and glycerol-3-phosphatase (GPP)) for a relatively long period while conducting oxygen limitation at a later stage to inhibit the increase of another enzyme activity related to glycerol degradation (i.e., mitochondrial glycerol-3-phosphate dehydrogenase (mtGPD)). With oxygen limitation performed from 88 h, when ctGPD and GPP activities were already at a low level while mtGPD activity was increasing, the glycerol dissimilation was efficiently reduced. The final glycerol concentration reached 55.6 g/L, which was about 18% (96 h) and 30% (104 h) higher than control, and its productivity increased to 0.54 g/(L h). The proposed strategy based on cell physiology was proved useful to the glycerol fermentation process.
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钟建江, Wei-Wei Hu, Hui Yao, and Jian-Jiang Zhong*
Biotechnol. Prog. 2001, 17, 838-846,-0001,():
-1年11月30日
A Panax notoginseng cell culture was successfully scaled up from shake flask to 1.0-L bubble column reactor and concentric-tube airlift reactor. High-density bioreactor batch cultivation was carried out using a modified MS medium. The maximum cell density in batch cultures reached 20.1, 21.0 and 24.1 g/L in the shake flask, bubble column and airlift reactors, respectively, and their corresponding biomass productivity was 950, 1140 and 1350 mg/(L
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钟建江, Qing-Hua Fang and Jian-Jiang Zhong*
Biotechnol. Prog. 2002, 18, 51-54,-0001,():
-1年11月30日
Investigations on the impact of pellet size on the cellular oxygen uptake and accumulation of ganoderic acid (GA) suggested the favorable effect of oxygen limitation on GA formation by the higher fungus Ganoderma lucidum. A two-stage fermentation process was thus proposed for enhanced GA production by combining conventional shake-flask fermentation with static culture. A high cell density of 20.9 g of DW/L (DW) dry cell weight was achieved through a 4-day shake-flask ermentation followed by a 12-day static culture. A change in the cell morphology and a decrease in the sugar consumption rate were observed during the static culture. The GA production in the new two-stage process was considerably enhanced with its content increased from 1.36 (control) to 3.19 mg/100 mg of DW, which was much higher than previously observed.
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钟建江, Zhi-Gang Qian, Zhen-Jiang Zhao, Yufang Xu, Xuhong Qian, , Jian-Jiang Zhong
BIOTECHNOLOGY AND BIOENGINEERING, VOL. 86, NO.7, JUNE 30, 2004,-0001,():
-1年11月30日
Novel hydroxyl-containing jasmonate derivatives were chemically synthesized and evaluated by bioassay as potential elicitors for stimulating the biosynthesis of plant secondary metabolites. A suspension culture of Taxus chinensis, which produces a bioactive taxoid, taxuyunnanine C (Tc), was taken as a model plant cell system. Experiments on the timing of addition of jasmonates and dose response indicated that day 7 and 100 AM was the optimal elicitation time and concentration, respectively, for both cell growth and Tc accumulation. Tc accumulation was increased more in the presence of novel hydroxylcontaining jasmonates compared to that with methyljasmonate (MJA) addition. For example, addition of 100 AM 2,3-dihydroxypropyl jasmonate on day 7 led to a very high Tc content of 47.2
plant cell culture, Taxus chinensis, synthetic jasmonate derivatives, inducing signal, secondary metabolism, plant defense response
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钟建江, Zhi-Gang Qian, Zhen-Jiang Zhao, Wen-Hong Tian, Yufang Xu, Jian-Jiang Zhong, Xuhong Qian
BIOTECHNOLOGY AND BIOENGINEERING, VOL. 86, NO.5, JUNE 5, 2004,-0001,():
-1年11月30日
Suspension cultures of Taxus chinensis were used as a model plant cell system to evaluate novel synthetic jasmonates as elicitors for stimulating the biosynthesis of secondary metabolites. Significant increases in accumulation of taxuyunnanine C (Tc) were observed in the presence of newly synthesized 2-hydroxyethyl jasmonate (HEJA) and trifluoroethyl jasmonate (TFEJA) without their inhibition on cell growth. Addition of 100 AM HEJA or TFEJA on day 7 led to a high Tc content of 44.3
elicitation, plant cell culture, Taxus chinensis, secondary metabolites, taxoid, novel synthetic jasmonates
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