王石平
(1)水稻抗病基因和抗病相关基因的鉴定、克隆、功能以及抗病信号传导的研究;(2)水稻基因组的进化。
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- 姓名:王石平
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学术头衔:
博士生导师
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学科领域:
农艺学
- 研究兴趣:(1)水稻抗病基因和抗病相关基因的鉴定、克隆、功能以及抗病信号传导的研究;(2)水稻基因组的进化。
王石平,教授,女,1977年毕业于华中农学院,1986年获英国爱丁堡大学哲学硕士学位,1994年获美国弗吉尼亚理工学院暨州立大学博士学位,现任华中农业大学教授,博士生导师。主要研究方向:(1)水稻抗病基因和抗病相关基因的鉴定、克隆、功能以及抗病信号传导的研究;(2)水稻基因组的进化。主要研究成绩:(1)根据各类分子标记在水稻染色体上的分布规律提出染色体复制在水稻基因组的进化中占有重要位置;该系列工作的研究结果获得2002年湖北省自然科学一等奖。(2)鉴定、精细定位和分离克隆了一批水稻抗病基因和抗病相关基因,为分子育种提供了基因资源和紧密连锁的分子标记。(3)揭示了几个水稻抗病基因和抗病相关基因的功能和特征,为阐明水稻抗病机理奠定了理论基础。(4)建立了一批水稻抗病QTL与抗病相关基因的对应关系,并揭示了水稻和大麦的数量抗病基因具有一定的染色体共线性关系,为研究植物数量抗性机理奠定了基础。
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王石平, Shiping Wang, Qifa Zhang, *, P.J. Maughan and M.A. Saghai Maroof
Plant Molecular Biology 33: 1051-1058, 1997.,-0001,():
-1年11月30日
Degenerated oligonucleotide primerswere used to amplify, clone, and analyze sequence heterogeneity and chromosomaldistribution of 23 PCR fragments corresponding to the reverse transcriptase domain of copia-like retrotransposonsin rice. Of the 23 fragments 22 could be aligned by their deduced amino acid sequences and were dividedinto 6 groups according to the phylogenetic and Southern blot analyses. Amino acid sequence differences among the22 aligned fragments ranged from 1 to 64%. Southern blot analysis of 10 rice accessions including indica, japonicaand common wild rice, using these 23 fragments as probes, showed that copia-like retrotransposons were presentin moderate to high copy numbers in all the rice genome although the exact copy number cannot be determined.The major difference revealed by southern analysis is a differentiation between the four indica varieties as onegroup and the four japonica varieties and the two wild rice accessions as another group. Polymorphisms were alsodetected among the indica and japonica varieties by major bands and repeatable minor bands. Five hybridizationbands were mapped to chromosomes 3, 4, 8, and 9, respectively. All the five bands were inherited in a dominantMendelian fashion and were not allelic with each other, indicating that the same element did not reside on the samelocation in different rice accessions. No transcript of the copia-like reverse transcriptase was detected on northernblot. The results suggest that the sequence heterogeneity and distributional variability of retrotransposons may beone of contributory factors causing genetic diversity in rice.
genetic diversity,, indica,, japonica,, mapping,, reverse trans, c, r, i, p, t, ase
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王石平, SHIPING WANG, NAN LIU, KAIMAN PENG, AND QIFA ZHANG†
Proc. Natl. Acad. Sci. USA Vol. 96, pp. 6824-6828, June 1999,-0001,():
-1年11月30日
We used 22 fragments corresponding to thereverse transcriptase domain of copia-like retrotransposonsas representatives to study the organization and distributionof these elements in the rice genome. The loci detected by these22 fragments were assigned to 47 locations in the molecularlinkagemap involving all 12 chromosomes. The distributionalfeatures of copia-like retrotransposons found in the ricegenome indicated that (i) the loci detected were located mainlyin one arm of each chromosome; (ii) one fragment usuallydetected several loci that were mapped to similar locations ofdifferent chromosomes; (iii) retrotransposons sharing highidentity in nucleotide sequences were usually assigned tosimilar locations of the chromosomes; and (iv) concurrencesof multiple loci, detected by different fragments, in similarlocations or stretches of different chromosomes were commonin the rice genome. We also determined that the copy numberof copia-like retrotransposons in rice genome may be as low as'100 per haploid genome. The restricted distribution, alongwith low copy number, suggested that copia-like retrotransposonsin rice were relatively inactive during evolution comparedwith those in other plants. The distributional featuresof the copia-like retrotransposons suggested the existence ofpossible lineages among the rice chromosomes, which in turnsuggested that chromosome duplication and diversificationmay be a mechanism for the origin and evolution of the ricechromosomes. The information provided by fine mapping ofthe retroelements in the genetic linkage map may also beuseful for gene tagging and molecular cloning.
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王石平, Huilan Chen, Shiping Wang, and Qifa Zhang
,-0001,():
-1年11月30日
Bacterial blight, caused by Xanthomonas oryzae pv. oryzae, is a seriousdisease of rice worldwide. A new dominant gene for bacterial blightresistance in rice, Xa25(t), was identified from Minghui 63, a restorerline for a number of rice hybrids that are widely cultivated in China. Thisgene conferred resistance to Philippine race 9 (PXO339) of X. oryzae pv. oryzae in both seedling and adult stages. It was mapped to the centromericregion of chromosome 12, 2.5cM from a disease resistance genehomologoussequence, NBS109, and 7.3cM from a restriction fragmentlength polymorphism marker, G1314. The genomic location of this geneis similar to the previously identified blast resistance genes, Pi-ta andPi-ta2.
mapping,, R gene.,
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王石平, Huilan Chen, Shiping Wang, and Qifa Zhang
,-0001,():
-1年11月30日
Bacterial blight, caused by Xanthomonas oryzae pv. oryzae, is a seriousdisease of rice worldwide. A new dominant gene for bacterial blightresistance in rice, Xa25(t), was identified from Minghui 63, a restorerline for a number of rice hybrids that are widely cultivated in China. Thisgene conferred resistance to Philippine race 9 (PXO339) of X. oryzae pv.centromericregion of chromosome 12, 2.5cM from a disease resistance genehomologoussequence, NBS109, and 7.3cM from a restriction fragmentlength polymorphism marker, G1314. The genomic location of this geneis similar to the previously identified blast resistance genes, Pi-ta andPi-ta2.
mapping,, R gene.,
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王石平, Xinli Sun, Yinglong Cao, Zhifen Yang, Caiguo Xu, Xianghua Li, Shiping Wang* and Qifa Zhang
The Plant Journal (2004) 37. 517-527,-0001,():
-1年11月30日
Rice bacterial blight, caused by Xanthomonas oryzae pv, oryzae (Xoo), is one of the most serious rice diseases worldwide, A rice gene, Xa26, conferring resistance against Xoo at both seedling and adult stages was isolated by map-based cloning strategies from the rice cultivar Minghui 63, Xa26 belongs to a multi-gene family consisting of four members, It encodes a leucine-rich repeat (LRR) receptor kinase-like protein and is constitutively expressed, Sequence analysis revealed that IRBB3 and Zhachanglong lines that are resistant to a broad range of Xoo strains, also carry Xa26, However, significant difference in lesion length was observed among these lines after inoculation with a set of Xoo strains, Moreover, transgenic plants carrying Xa26 showed enhanced resistance compared with the donor line of the gene in both seedling and adult stages, These results suggest that the resistance conferred by Xa26 is influenced by the genetic background.
R gene,, Xoo,, bacterial blight disease,, receptor kinase protein,, multigene family,, rice,,
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王石平, J. Jiang
Mol Genet Genomics (2002) 268: 249-252,-0001,():
-1年11月30日
Rice blast disease, caused by the fungalpathogen Pyricularia grisea Sacc., is one of the mostdevastating crop diseases worldwide. Previous studieshave shown that the dominant blast resistance genePi-2(t) confers resistance to a broad spectrum ofpathogenic strains. Using a population of 292 recombinantinbred lines combined with bioinformatic analysis,we mapped Pi-2(t) between the SSR (simple-sequencerepeat) marker SSR140 and the RFLP (restrictionfragment length polymorphism) marker JSH12, 0.9 cMfrom both SSR140 and JSH12. A physical map consistingof six overlapping BAC (bacterial artificialchromosome) clones was anchored to the region containingthe Pi-2(t) locus. By analyzing recombinationevents in this region, the Pi-2(t) locus was localized to aDNA fragment of 118 kb in length. The detailed geneticand physical maps of the Pi-2(t) locus will facilitateboth molecular isolation of the gene and marker-assistedtransfer of the gene in breeding programs.
Pi-2(, t),
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王石平, Huilan Chen*†, Shiping Wang*†, Yongzhong Xing*, Caiguo Xu*, Patrick M. Hayes‡, and Qifa Zhang*§
PNAS March 4, 2003, Vol. 100 no.5, 2544-2549,-0001,():
-1年11月30日
Comparative genomic analyses have revealed extensive colinearity ingene orders in distantly related taxa in mammals and grasses, whichopened new horizons for evolutionary study. The objective of ourstudy was to assess syntenic relationships of quantitative trait loci(QTL) for disease resistance in cereals by using a model system inwhich rice and barley were used as the hosts and the blast fungusPyricularia grisea Sacc. as the pathogen. In total, 12 QTL against threeisolates were identified in rice; two had effects on all three isolates,and the other 10 had effects on only one or two of the three isolates.Twelve QTL for blast resistance were identified in barley; one hadeffect on all three isolates, and the other 11 had effects on only oneor two of the three isolates. The observed isolate specificity led to ahypothesis about the durability of quantitative resistance commonlyobserved in many plant host-pathogen systems. Four pairs of the QTLshowed corresponding map positions between rice and barley, twoof the four QTL pairs had complete conserved isolate specificity, andanother two QTL pairs had partial conserved isolate specificity. Suchcorresponding locations and conserved specificity suggested a commonorigin and conserved functionality of the genes underlying theQTL for quantitative resistance and may have utility in gene discovery,understanding the function of the genomes, and identifying theevolutionary forces that structured the organization of the grassgenomes.
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王石平, X. Sun
Theor Appl Genet (2003) 106: 683-687,-0001,():
-1年11月30日
Bacterial blight caused by Xanthomonas oryzaepv oryzae is a devastating disease in rice worldwide.The resistance gene Xa4 has been widely used in breedingprograms and played an important role in protectingrice from this disease. Using 642 highly susceptible individualsand a random sample of 255 individuals from anF2 population developed from a cross between IRBB4and IR24, the Xa4 gene was genetically mapped to aregion less than 1 cM. A contig map was constructed forthe Xa4 region consisting of six non-redundant bacterialartificial chromosome (BAC) clones and spanning approximately500 kb in length. Analysis of recombinationevents in the Xa4 region located the gene locus to oneBAC, 3H8. Assay of the recombinants using the subclonesof 3H8 in combination with sequence analysisfurther narrowed the Xa4 locus down to a 47-kb fragment.
R gene
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【期刊论文】Genetic and physical mapping of a new gene for bacterial blight resistance in rice
王石平, Z. Yang
Theor Appl Genet (2003) 106: 1467-1472,-0001,():
-1年11月30日
The inheritance of resistance for bacterialblight, caused by Xanthomonas oryzae pv. oryzae (Xoo),was studied in Minghui 63, an elite restorer line for anumber of widely used rice hybrids in China. A newdominant gene against a Chinese Xoo strain JL691 in boththe seedling and adult stages was identified in Minghui 63and designated as Xa26(t). Using a total of 477 highlysusceptible individuals from an F2 population, the Xa26(t)locus was mapped to a region of about 1.68 cM. Thislocus co-segregated with marker R1506 and was 0.21 cMfrom marker RM224 on one side and 1.47 cM frommarker Y6855RA on the other side, in rice chromosome11. A contig map, composed of five non-redundantbacterial artificial chromosome (BAC) clones and spanningapproximately 500 kb in length, was constructed.Analysis of recombination events in the Xa26(t) regionwith the highly susceptible F2 individuals anchored thegene locus to a region covered by three overlapped BACclones. Assay of the lines showing a double crossover inmarker loci flanking Xa26(t), in a population of recombinantinbred lines carrying Xa26(t), further delineatedthe gene to a 20-kb fragment. The Xa26(t) locus is tightlylinked to another bacterial blight resistance gene locus,Xa4.
Bacterial blight
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王石平, N. Wen
Mol Gen Genomics (2003) 269: 331-339,-0001,():
-1年11月30日
Bacterial blight and fungal blast diseases ofrice, caused by Xanthomonas oryzae pv. oryzae andPyricularia grisea Sacc., respectively, are two of the mostdevastating diseases in rice worldwide. To study thedefense responses to infection with each of thesepathogens, expression profiling of 12 defense-responsivegenes was performed using near-isogenic rice lines thatare resistant or susceptible to bacterial blight and fungalblast, respectively, and rice cultivars that are resistant orsusceptible to both pathogens. All 12 genes showedconstitutive expression, but expression levels increasedin response to infection. Based on their expression patternsin 12 host-pathogen combinations, these genescould be classified into three types, pathogen non-specific(6), pathogen specific but race non-specific (4) andrace specific (2). Most of the 12 genes were only responsiveduring incompatible interactions. These resultssuggest that bacterial blight and fungal blast resistancesshare common pathway(s), but are also regulated bydifferent defense pathways in rice. Activation of thecorresponding R gene is the key step that initiates theaction of these genes in defense responses. The chromosomallocations and pathogen specificities of seven ofthe 12 genes were consistent with those of previouslyidentified quantitative trait loci for rice disease resistance,which indicates that some of the 12 genes studiedmay have a phenotypic impact on disease resistance inrice.
Defense-related genes
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