严小军
海洋生物药源生物学与生物代谢分析,侧重于海洋植物的次生代谢产物分析与生物学活性的研究与开发。
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- 姓名:严小军
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学术头衔:
博士生导师
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学科领域:
海洋生物学
- 研究兴趣:海洋生物药源生物学与生物代谢分析,侧重于海洋植物的次生代谢产物分析与生物学活性的研究与开发。
严小军,男,1968年8月生,1989年7月毕业于复旦大学化学系,获理学学士, 1994年8月获中国科学院海洋研究所海洋生物学专业理学博士学位。1996年10月-1999年2月在日本国立食品综合研究所任博士后,2000年10月-2002年2月美国麻省大学生物化学系访问教授。曾任中国科学院海洋研究所“知识创新工程”实验海洋生物学开放实验室海洋药源生物学责任研究员、中国科学院海洋研究所博士生导师。2002年6月起,任宁波大学生命学院教授(浙江省高校特聘教授)、浙江省海洋生物工程重点实验室主任。是中国藻类学会的常务理事,新世纪百千万人才工程国家级人选,浙江省151人才工程第一层次人选,是宁波大学海洋生物学硕士点的负责人。
研究方向:海洋生物药源生物学与生物代谢分析,侧重于海洋植物的次生代谢产物分析与生物学活性的研究与开发。
主要业绩:在承担国家科技部八五、九五攻关计划期间,详细研究了中国沿海主要经济海藻中碘元素与其它20多种微量的资源分布、化学存在形态,发现了海藻中有机碘主要以蛋白质结合态与多酚结合态为主,从而提出了海藻中有机碘的提取技术,参与研制开发了“海藻活性碘”系列产品,在防治碘缺乏病中取得了重要成绩。在承担国家重点基础研究项目、国家自然科学基金重点项目与国际科技合作中,对于30多种海洋植物天然抗氧化成分进行了比较系统的研究,首次发现高分子量褐藻多酚具有显著的抗氧化活性与植物化学防御作用。解明了40多个具有抗肿瘤、抗氧化活性的寡糖化合物、酚类化合物、类胡萝卜素、三萜皂苷、生物碱等,丰富了海洋天然产物研究成果,发表的论文被SCI引用70多次。
创造了海带富硒生化新技术,并成功地实现了新技术的产业化,研究结果不仅发表在该领域权威杂志《美国农业与食品化学杂志》,申报了两项发明专利,而且以此为基础开发了近十个新产品,为海带养殖加工产业链的延长与开拓做出了突破性进展。
曾获得中国科学院科技进步一等奖,日本科学技术厅外国人研究奖励,宁波市十大青年科技创新奖、宁波市十大杰出青年、国家海洋局优秀图书奖等奖励,在国内外核心学术期刊发表论文100余篇,其中SCI论文18篇,申请及获得发明专利7项,参加撰写及编著学术专著5部。
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710
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成果数
12
严小军, Li Zheng, Haimin Chen, Xiaotian Han, Wei Lin and Xiaojun Yan, *
World Journal of Microbiology & Biotechnology 2005 21: 201-206,-0001,():
-1年11月30日
Twenty-nine marine bacterial strains were isolated from the sponge Hymeniacidon perleve at Nanji island, and antimicrobial screening showed that eight strains inhibited the growth of terrestrial microorganisms. The strain NJ6-3-1 with wide antimicrobial spectrum was identified as Pseudoalteromonas piscicida based on its 16S Rrna sequence analysis. The major antimicrobial metabolite, isolated through bioassay-guide fractionation of TLC bioautography overlay assay, was identified as norharman (a beta-carboline alkaloid) by EI-MS and NMR.
Antimicrobial activity,, beta-carboline,, marine bacteria,, sponge
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严小军, Hai-Min Chena, b, Li Zheng a, Wei Lin a, Xiao-Jun Yan c, ∗
Talanta 64(2004)773-777,-0001,():
-1年11月30日
Since the discovery of multiple bioactivities for agarobiose oligomers, a quantitative method has been in great need to monitor the agarobiose oligomers. This report demonstrates that agarobiose oligomers can be separated with high resolution in HPLC after introducing α-naphthylamine into compounds. Agarobiose oligomers ranged from biose to decaose were isolated by Sephadex column. HPLC analysis indicated that each oligomer could be quantified with good linearity and a low detection limit of 0.1-4µg/ml. The chromatographic profiles of agaro-oligosaccharides with different hydrolysis modes (hydrochloride, citric acid, solid acid, and hydroxyl radical degradation) showed that agarobiose could be obtained more than 57.8% using solid acid mediated hydrolysis, while hydrochloride acid could degrade agar into a series of agaro-oligosaccharides from biose to decaose. The yield of oligosaccharides was low if hydrolyzed by citric acid. The Fenton degradation can increase the speed of hydrolysis, but the product was complex.
Agarobiose oligomers, HPLC, α-Naphthylamine, Agaro-oligosaccharides
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【期刊论文】A New Method for Screening a-Glucosidase Inhibitors and Application to Marine Microorganisms
严小军, Haimin Chen, Xiaojun Yan, Wei Lin, Li Zheng and Weiwei Zhang
Pharmaceutical Biology 2004, Vol. 42, No.6, pp. 416-421,-0001,():
-1年11月30日
A new enzyme assay method for screening a-glucosidase inhibitors with rapidity and simplicity was developed. The enzyme-substituted a-glucosidases for this assay was glucoamylase. Samples were spotted or developed on the silica gel plate. The agar solution containing substrate was poured on the plate, and paper impregnated with enzyme was layered on the agar. After incubation, an inhibitory circle would appear around the inhibitor. By using this method, more than 200 strains of marine microorganisms were screened. Among them, three active strains were found to secrete inhibitors in the culture medium.
Enzyme assay,, a-glucosidase inhibitor,, marine microorganism.,
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【期刊论文】Extraction and Identification of Antioxidants in the Roots of Yacon (Smallanthus sonchifolius)
严小军, Xiaojun Yan, †, Masahiro Suzuki, Mayumi Ohnishi-Kameyama, *, Yasutoshi Sada, ‡, Tateo Nakanishi, §, and Tadahiro Nagata†
J. Agric. Food Chem. 1999, 47, 4711-4713,-0001,():
-1年11月30日
Yacon, Smallanthus sonchifolius (Poepp. & Endl.) H. Robinson, Asteraceae, an important economic species grown for its juicy tuberous root, is potentially beneficial in the diet to diabetics. The antioxidative activity of yacon root was studied by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Antioxidants were extracted by methanol and isolated and purified by gel permeation chromatography and preparative reverse-phase HPLC. Two of the major antioxidants were identified as chlorogenic acid and tryptophan by NMR and mass spectrometry.
Yacon, Smallanthus sonchifolius, antioxidant, chlorogenic acid, tryptophan, Asteraceae
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【期刊论文】Determination of Synthetic Hydroxytyrosol in Rat Plasma by GC-MS
严小军, Chen Bai, †, Xiaojun Yan, Makiko Takenaka, Keizo Sekiya, ‡, and Tadahiro Nagata*
J. Agric. Food Chem. 1998, 46, 3998-4001,-0001,():
-1年11月30日
2-(3,4-Dihydroxyphenyl)ethanol (DPE), the major phenolic compound in olive oil, may contribute the antioxidative activities and other beneficial effects to olive oil. However, the lack of commercial available DPE and procedures sensitive enough to quantitatively determine DPE in body fluids have limited the bioavailability and metabolism studies on this phenolic compound. In the present study, DPE was synthesized with high yield and high purity and administered orally to rats. DPE concentration in rat plasma, after absorption, was measured using a sensitive GC-MS-SIM method. The results indicated that the highest level of DPE in plasma was detected at 5-10 min after administration. During this period, the concentration of DPE fluctuated widely with the individual.
Hydroxytyrosol, dihydroxyphenylethanol (, DPE), , plasma, GC-MS, metabolism
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严小军, XIAOJUN YAN, †, ‡, BRIAN T. MURPHY, GERALD B. HAMMOND, JOE A. VINSON, §, AND CATHERINE C. NETO*
J. Agric. Food Chem. 2002, 50, 5844-5849,-0001,():
-1年11月30日
ng seven tumor cell lines. Selective inhibition of K562 and HT-29 cells was observed from a methanolic extract in the range of 16-125 μg/mL. Radicalscavenging activity was greatest in an extract composed primarily of flavonol glycosides. Seven flavonol glycosides were isolated and purified from whole fruit for further evaluation; the anthocyanin cyanidin 3-galactoside was also purified for comparison with the flavonoids. Three flavonol monoglycosides were newly identified by 13C NMR as myricetin 3-α-arabinofuranoside, quercetin 3-xyloside, and 3-methoxyquercetin 3-β-galactoside (isorhamnetin); the other four isolated were the previously identified myricetin 3-β-galactoside, quercetin 3-β-galactoside, quercetin 3-α-arabinofuranoside, and quercetin 3-α-rhamnopyranoside. These compounds were evaluated for 1,1-diphenyl-2-picrylhydrazyl radical-scavenging activity and ability to inhibit low-density lipoprotein oxidation in vitro. Most of the flavonol glycosides showed antioxidant activity comparable or superior to that of vitamin E; cyanidin 3-galactoside showed activity superior to that of the flavonoids as well as vitamin E or Trolox in both antioxidant assays.
Antioxidant, antitumor, cranberry, flavonoid, anthocyanin, phenolic, radical scavenger
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严小军, BRIAN T. MURPHY, †, SHAWNA L. MACKINNON, ‡, XIAOJUN YAN, §, GERALD B. HAMMOND, ABRAHAM J. VAISBERG, ║, AND CATHERINE C. NETO*
J. Agric. Food Chem. 2003, 51, 3541-3545,-0001,():
-1年11月30日
Bioactivity-guided fractionation of cranberry fruit was used to determine the identity of triterpenoid esters from Vaccinium macrocarpon, which inhibit tumor cell growth and may play a role in cancer prevention. In our previous study, a fraction from whole fruit exhibited tumor cell growth inhibition in vitro. The major components of this fraction were isolated by chromatographic separation of ethyl acetate extracts, purified by semipreparative HPLC, and identified by NMR as cis- (1) and trans- (2) isomers of 3-O-p-hydroxycinnamoyl ursolic acid. These triterpenoid esters have not been previously reported in Vaccinium fruit. Bioassay of the purified triterpene cinnamates in tumor cell lines in vitro showed slightly greater activity of compound 1 in most cell lines, with GI50 values of approximately 20µM in MCF-7 breast, ME180 cervical and PC3 prostate tumor cell lines. Quercetin was slightly less active than 1, while cyanidin-3-galactoside exhibited much lower cytotoxicity, with GI50 greater than 250 μM in all cell lines. Phenylboronic acid (3) was also isolated from the fruit but showed insignificant antitumor activity.
antitumor, cranberry, triterpenoids, hydroxycinnamic acid, cancer, cytotoxic, ursolic acid esters
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【期刊论文】Caffeic Acid Derivatives in the Roots of Yacon (Smallanthus sonchifolius)
严小军, MAKIKO TAKENAKA, *, †, XIAOJUN YAN, ‡, HIROSHI ONO, MITSURU YOSHIDA, TADAHIRO NAGATA, AND TATEO NAKANISHI§
J. Agric. Food Chem. 2003, 51, 793-796 ,-0001,():
-1年11月30日
Five caffeic acid derivatives were found in the roots of yacon, Smallanthus sonchifolius (Poepp. And Endl.) H. Robinson, Asteraceae, as the major water-soluble phenolic compounds. The structures of these compounds were determined by analysis of spectroscopic data. Two of these were chlorogenic acid (3-caffeoylquinic acid) and 3,5-dicaffeoylquinic acid, common phenolic compounds in plants of the family Asteraceae. Three were esters of caffeic acid with the hydroxy groups of aldaric acid, derived from hexose. The structure of the aldaric moiety was determined by hydrolysis and comparison of NMR spectra with those of standard aldaric acids. The compounds were novel caffeic acid esters of altraric acid: 2,4-or 3,5-dicaffeoylaltraric acid, 2,5-dicaffeoylaltraric acid, and 2,3,5-or 2,4,5- tricaffeoylaltraric acid.
Yacon, Smallanthus sonchifolius, Asteraceae, caffeic acid, altraric acid
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严小军, XIAOJUN YAN, *, † LI ZHENG, §, HAIMIN CHEN, WEI LIN, AND WEIWEI ZHANG§
J. Agric. Food Chem. 2004, 52, 6460-6464,-0001,():
-1年11月30日
Accumulations of selenium in kelp Laminaria japonica cultured in seawater was achieved by adding selenite (Na2SeO3) with or without N-P (NaNO3+NaH2PO4) nutrients at different concentrations. Biotransformation of selenium in the kelp was investigated through measuring the selenium of biological samples and different biochemical fractionations. The results showed that the optimal seleniteenrichment concentration is 200 mg L-1, which can allow the kelp to accumulate a total selenium content from 0.51 (0.15
Selenium, seaweed, accumulation, biotransformation
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【期刊论文】Determination of chemical species of iodine in some seaweeds (I)
严小军, Xiaolin Hou a, *, Chifang Chai a, Qinfang Qian a, Xiaojun Yan b, Xiao Fan b
The Science of the Total Environment 204(1997)215-221,-0001,():
-1年11月30日
The chemical species of iodine in seven marine algae Codium fragile, Ulva pertusa, Monostroma nitidum, Gracilaria confervoides, Sargassum Kjellmanianum, Dictyopteris divaricata and Laminaria japonica were studied using neutron activation analysis combined with chemical separation. The contents of total iodine, water-soluble iodine, soluble organic iodine, I- and 103 were determined. The results indicate that the chemical species and contents of iodine in various algae are remarkably different. The highest iodine content of 734 mg/kg (wet basis) was found in Laminaria japonica, with 99.2% of the total iodine being water soluble. The iodine contents of the other six algae are. lower and soluble iodine makes up 16-41% of the total. In the aqueous leachate, iodine is mainly I-, which amounts to 61-93% of total water-soluble iodine; the percentages of organic iodine making up 5.5-37.4%, while the contents of IO3- are the lowest, 1.4-4.5%. This result suggests that the mechanism of iodine enrichment is different for various algae and that its bioavailability varies as well.
Marine algae, Iodine, Chemical species, Neutron activation analysis, Bioavailability
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