彭宣宪
主要兴趣在适应性免疫分子的起源和进化以及微生物多价疫苗的研究等方面。
个性化签名
- 姓名:彭宣宪
- 目前身份:
- 担任导师情况:
- 学位:
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学术头衔:
博士生导师
- 职称:-
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学科领域:
细胞生物学
- 研究兴趣:主要兴趣在适应性免疫分子的起源和进化以及微生物多价疫苗的研究等方面。
1954年12月生,男,教授,博士,博士生导师,教育部科技委学部委员,中国生物化学与分子生物学学会海洋生物化学与分子生物学分会副理事长,中国细胞生物学会常务理事基因组和蛋白质组专业委员会副主任,国家海洋局海洋生物遗传资源重点实验室学术委员,教育部生物农药与化学生物学重点实验室学术委员,教育部细胞生物学与肿瘤细胞工程重点实验室学术委员,福建省细胞生物学学会理事长,福建省生物工程学会副会长,厦门大学学位委员会委员,厦门大学学报(自然科学版)副主编, Inter J Biochem Cell Biol杂志审稿人。1987年毕业于西安医科大学微生物学与免疫学专业;其后在江西大学生物学系任教,1993年5月晋升为教授;1993年-1994年在加拿大McGill大学从事博士后研究;回国后任教于南昌大学(原江西大学),担任生物工程研究所所长;1996年至今任教于厦门大学,1997年5月-1999年12月担任生物学系主任,1999年12月-2004年2月担任首任生命科学学院院长兼生物学系主任(工作业绩略)。1998.06-1998.09系日本京都大学研究生院访问教授; 2001.06-2001.09系美国Scripps研究所访问教授。先后主持完成国际、国家和省级课题16项;出版著作4部;通过居国际先进水平的鉴定成果2项(均为第一完成者);发表学术论著120余篇,最高影响因子15(J. Exp. Med. 1999;189: 1101-1110);获省部级科技奖3项、省教学成果一等奖和国家级教学成果二等奖各1项(均为第一获奖者)。有关论著被国内外权威学术刊物反复引用(据不完全统计,被引用400余次)。应邀访问了美国、加拿大、日本和英国等六所大学。获得全国劳动模范、国务院特殊津贴、全国中青年新闻人物、省优秀留学归国人员、省劳动模范、省中青年学科带头人、省三育人(教学、科研、管理)先进个人、省优秀教育工作者、厦门市拔尖人才等荣誉称号。目前主要兴趣在适应性免疫分子的起源和进化以及微生物多价疫苗的研究等方面。并在国内率先建立了以海洋生物和微生物为主要对象的蛋白质组学研究平台(1999年开始)。
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主页访问
3102
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成果阅读
271
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成果数
5
彭宣宪, Xuanxian Peng, * Xintai Ye, Sanying Wang
Vaccine 22(2004)2750-2756,-0001,():
-1年11月30日
Shigella spp. are one of the most important etiological factors for people who are living in developing countries and travelers to tropical countries. High priority has been given by the World Health Organization to the development of vaccines to control Shigellosis caused by these bacteria. However, information regarding to profile of immunogenic proteins of Shigella is not available now. In the present study, sub-immunoproteomics was applied to screen novel immunogenic proteins which could be reacted with antisera produced by challenge of a whole bacterium. Our results indicated that 13 immunogens were identified, in which seven proteins and six proteins from outer membrane and soluble proteome, respectively. Of the 13 proteins, 12 showed to be novel immunogens. These results suggest that immunoproteomics can greatly improve the chances of identification and result in discovery of novel immunogenic proteins.
Proteomics, Vaccine, Shigella, Immunogen
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彭宣宪, Xuanxian Peng, Yajun Wu, Jingan Chen, Sanying Wang
Proteomics 2004, 4, 0000-0000,-0001,():
-1年11月30日
Proteome analysis by two-dimensional gel electrophoresis (2-DE) together with mass spectrometry was applied to screen acute phase response (APR)-related proteins with low molecular weight in loach skin following injury. Furthermore, Western blotting and function tests were applied to confirm the results obtained from the proteomic study. Fifteen APR-related proteins with sixteen spots (PLA with two spots) on a 2-DE map were identified in this study. Furthermore, six were knownacute phase proteins including galactose-binding lectin (GBL), lysozyme, C3, CD59, double PLA and 50s ribosomal protein; while ATP kinase, zinc finger protein 183, alpha-neurotoxin homology, angiostatin, serine/threonine kinase, metalloproteinase inhibitor, regulator of G-protein 4, cryptdin-9 and disintegrin trigranin were found by our lab to be APR-related proteins. In addition, our results suggest that proteomes with low molecular weight can be characterized by 2-DE with a Tris-tricine system followed by mass spectrometry.
Acute phase protein/, Acute phase reaction/, Loach/, Skin
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【期刊论文】Rapid screening of highly efficient vaccine candidates by immunoproteomics
彭宣宪, Zijun Chen, Bo Peng, Sanying Wang, Xuanxian Peng
Proteomics 2004, 4, 3203-3213,-0001,():
-1年11月30日
Diseases caused by microorganisms can be controlled by vaccines, which require neutralizing antigens. Therefore, it is very important to identify highly efficient immunogens for immune prevention. By combining immunoproteomics and bacterial challenge after immunization, we developed a rapid method for screening protected antigens of pathogenic bacteria in aquaculture. Our approach may be divided into three consecutive steps. First, dominant immunogens of outer membrane proteins are screened by immunoproteomics. Second, proteins with the ability to induce production of neutralizing antibodies are identified from the immunogens by virulent bacterium challenge following vaccination. Third, vaccine candidates are determined by evaluation of neutralizing abilities. Information on the candidates has been obtained for further gene cloning by mass spectrometry. Our results indicate that highly efficient protected antigens were identified from the outer membrane proteome of Aeromonas hydrophila, in which an immunogen showed 71.4% protective ability with multivalent functions to A. hydrophila and Aeromonas sobria. In summary, we have developed a high-throughout, accurate, rapid and highly efficient method which will play an active role in immune prevention for microbiological diseases.
Aeromonas hydrophila/, Immune prevention/, Immunoproteomics/, Outer membrane proteins/, Vaccine
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彭宣宪, Guolin Zhang a, Guizhong Wang b, Sanying Wang a, Qifu Li a, Gaoliang Ouyang a, Xuanxian Peng a, *
The International Journal of Biochemistry & Cell Biology 36(2004)1613-1623,-0001,():
-1年11月30日
Human gastric carcinoma BGC-823 cells underwent morphological differentiation and cell cycle arrest in vitro when treated with 5 mM hexamethylene bisacetamide (HMBA) for 48 h. To further understand the mechanism of HMBA-induced differentiation, proteomic methodologies were applied to screen and identify altered proteins involved in the commitment of BGC-823 cells to differentiate. Five distinct altered proteins were acquired by two-dimensional (2-D) PAGE and were consequently identified as ras-related protein rab-35 (Rab-35), splice truncated isoform of transmembrane protease, serine 3 (serine TADG-12), regulator of G-protein signaling 1 (RGS1), ret finger protein-like 1 (RFPL1) and F-actin capping protein alpha-3 subunit (GSG3) by analysis of mass spectrograph. Of the five proteins, serine TADG-12 down-regulated under the detectable level after HMBA treatment, Rab-35, RGS1 and RFPL1 sharply up-regulated within the HMBA-induced BGC-823 cells, and GSG3, appearing in both treated and untreated cells, remarkably increased within BGC-823 cells after HMB Astimulation. Our results implicate that the molecular mechanism of BGC-823 cell differentiation in response to HMBA may involved in complex processes including a signaling network linking vesicle transport, actin cytoskeleton remodeling except for morphology differentiation, cell cycle G1 arrest.
HMBA, Induction differentiation, Human gastric carcinoma BGC-823 cells, Proteomics
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彭宣宪, Xuanxian Peng*, Wen Luo, Jianying Zhang, Sanying Wang, and Shengcai Lin
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, May 2002, p. 2580-2583,-0001,():
-1年11月30日
In the present study, we developed a quick, highly specific method for detection of Shigella species by combining immunocapturing of the bacteria and a universal primer PCR. The method drastically enhances test sensitivity, and it can be used not only for identification of Shigella species in the environment but also for rapid detection of other pathogens.
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