艾华
从事新型纳米生物材料在分子影像和药物控释中应用的研究,开发了具肿瘤靶向的高灵敏度新型磁共振造影剂和纳米药物载体;在此基础上,对影像跟踪药物载体、影像跟踪细胞、分子影像探针与细胞的相互作用机理等方面开展了深入的研究
个性化签名
- 姓名:艾华
- 目前身份:
- 担任导师情况:
- 学位:
-
学术头衔:
博士生导师
- 职称:-
-
学科领域:
材料科学
- 研究兴趣:从事新型纳米生物材料在分子影像和药物控释中应用的研究,开发了具肿瘤靶向的高灵敏度新型磁共振造影剂和纳米药物载体;在此基础上,对影像跟踪药物载体、影像跟踪细胞、分子影像探针与细胞的相互作用机理等方面开展了深入的研究
艾华,男,1973年生,四川大学国家生物医学材料工程技术研究中心教授、博士生导师;全国青联常委。中国生物物理学会分子影像专业委员会常委;中国生物材料委员会委员;中国国际科学交流基金会专家委员会委员;美国化学学会会员;国家自然基金委评审专家;国家863项目评审专家。于北京中医药大学学习获医学学士学位(1991-1996)、于美国路易斯安那理工大学生物医学工程系学习获博士学位(1997-2002)、美国凯斯西储大学生物医学工程系做Research Associate兼博士后(2002-2005)。从2005年至今任四川大学教授。现担任生物材料领域顶级杂志Biomaterials(IF:7.882)杂志编委,Adv. Drug Deliv. Rev.、Biomaterials及一系列相关领域杂志评委。曾应邀担任美国国家宇航局(NASA)资助的科研项目(Nanoparticle Delivery of Repair Enzymes for Radiation Protection/DNA repair)顾问。先后参加多个美国NIH和NSF资助的重大科研项目,并作为负责人承担美国国防部基金项目。回国后主持了国家自然科学基金、教育部新世纪优秀人才计划、四川省杰出青年基金等项目。作为四川大学985工程“生物医学工程与技术科技创新平台”药物控释方向学术带头人,国家重点一级学科“生物医学工程”药物控释方向学术带头人,国家重点学科“影像医学”分子影像方向学术带头人(四川大学华西医院),国家973项目(组织诱导性生物医用材料的基础研究-装载基因材料的分子设计及组织诱导作用和机理,2005-2010)四川大学第一主研,积极开展国内外多学科合作的科研工作。获得2006年“四川省有突出贡献的优秀专家”称号。
艾华教授主要从事新型纳米生物材料在分子影像和药物控释中应用的研究,开发了具肿瘤靶向的高灵敏度新型磁共振造影剂和纳米药物载体;在此基础上,对影像跟踪药物载体、影像跟踪细胞、分子影像探针与细胞的相互作用机理等方面开展了深入的研究。相关成果发表在Acc. Chem. Res.(影响因子:21.840)、Adv. Drug Deliv. Rev.(影响因子:13.577)、Adv. Mater.(影响因子:10.857)、Biomaterials(影响因子:7.882)、Nano Letter.(影响因子:12.186)、Small(影响因子:7.333)、Angew. Chem. Inter. Ed.(影响因子:12.730)等国际知名期刊上,并得到MIT Technology Review杂志和美国国家卫生署NIH Monthly Feature的专题报道,被他引1100余次,并受到不少国际一流学者的关注。其中,关于高灵敏度探针的研究,得到了国际知名影像专家、华盛顿大学医学院Samuel A. Wickline和Gregory M. Lanza教授的长篇幅引用,指出“该探针具备长时间影像窗口观察的优势,有望用于肝脏微小病变的发现和鉴别诊断”。相关著作由国际知名的CRC出版社、American Scientific Publisher、及World Scientific Publishing Co. 出版。应邀在2010年美国控释学会年会上做主题发言(Keynote Speaker);2011年应邀在美国布朗大学做学术报告;应邀撰写综述文章,已在药物控释领域顶级期刊Advanced Drug Delivery Reviews和美国化学学会权威综述杂志Accounts of Chemical Research上发表。
-
主页访问
3335
-
关注数
0
-
成果阅读
621
-
成果数
12
艾华, Norased Nasongkla, † Erik Bey, † Jimin Ren, ‡ Hua Ai, † Chalermchai Khemtong, † Jagadeesh Setti Guthi, † Shook-Fong Chin, † A. Dean Sherry, ‡ David A. Boothman, † and Jinming Gao*, †
NANO LETTERS 2006 Vol.6, No.11 2427-2430,-0001,():
-1年11月30日
We describe the development of multifunctional polymeric micelles with cancer-targeting capability via rvâ3 integrins, controlled drug delivery, and efficient magnetic resonance imaging (MRI) contrast characteristics. Doxorubicin and a cluster of superparamagnetic iron oxide (SPIO) nanoparticles were loaded successfully inside the micelle core. The presence of cRGD on the micelle surface resulted in the cancer-targeted delivery to rvâ3-expressing tumor cells. In vitro MRI and cytotoxicity studies demonstrated the ultrasensitive MRI imaging and rvâ3-specific cytotoxic response of these multifunctional polymeric micelles.
-
50浏览
-
0点赞
-
0收藏
-
0分享
-
118下载
-
0评论
-
引用
艾华, Xintao Shuaia, , Hua Aia, Norased Nasongklab, Saejeong Kima, Jinming Gaoa, *
Journal of Controlled Release 98 (2004) 415-426,-0001,():
-1年11月30日
Diblock copolymers of poly(q-caprolactone) (PCL) and monomethoxy poly(ethylene glycol) (MPEG) with various compositions were synthesized. The amphiphilic block copolymers self-assembled into nanoscopic micelles and their hydrophobic cores encapsulated doxorubicin (DOX) in aqueous solutions. The micelle diameter increased from 22.9 to 104.9 nm with the increasing PCL block length (2.5-24.7 kDa) in the copolymer composition. Hemolytic studies showed that free DOX caused 11% hemolysis at 200 Ag ml 1, while no hemolysis was detected with DOX-loaded micelles at the same drug concentration. An in vitro study at 37 jC demonstrated that DOX-release from micelles at pH 5.0 was much faster than that at pH 7.4. Confocal laser scanning microscopy (CLSM) demonstrated that DOX-loaded micelles accumulated mostly in cytoplasm instead of cell nuclei, in contrast to free DOX. Consistent with the in vitro release and CLSM results, a cytotoxicity study demonstrated that DOX-loaded micelles exhibited time-delayed cytotoxicity in human MCF-7 breast cancer cells.
Polymer micelles, Drug delivery
-
80浏览
-
0点赞
-
0收藏
-
0分享
-
89下载
-
0评论
-
引用
【期刊论文】Nano-encapsulation of furosemide microcrystals for controlled drug release
艾华, Hua Ai a, Steven A. Jones a, Melgardt M. de Villiers b, Yuri M. Lvov a, *
Journal of Controlled Release 86 (2003) 59-68,-0001,():
-1年11月30日
Furosemide microcrystals were encapsulated with polyions and gelatin to control the release of the drug in aqueous solutions. Charged linear polyions and gelatin were alternatively deposited on 5-mm drug microcrystals through layer-bylayer (LbL) assembly. Sequential layers of poly(dimethyldiallyl ammonium chloride) (PDDA) and poly(styrenesulfonate) (PSS) were followed by adsorption of two to six gelatin /PSS bilayers with corresponding capsule wall thicknesses ranging from 45 to 115 nm. The release of furosemide from the coated microparticles was measured in aqueous solutions of pH 1.4 and 7.4. At both pH values, the release rate of furosemide from the encapsulated particles was reduced by 50–300 times (for capsules coated with two to six bilayers) compared to uncoated furosemide. The results provide a method of achieving prolonged drug release through self-assembly of polymeric shells on drug microcrystals.
Nano-encapsulation, Controlled release, Furosemide, Self-assembly
-
34浏览
-
0点赞
-
0收藏
-
0分享
-
135下载
-
0评论
-
引用
【期刊论文】Electrostatic Layer-by-Layer Nanoassembly on Biological Microtemplates: Platelets
艾华, Hua Ai, † Ming Fang, ‡ Steven A. Jones, † and Yuri M. Lvov*, ‡
Biomacromolecules 2002, 3, 560-564,-0001,():
-1年11月30日
Platelets were coated with 78-nm silica nanoparticles, 45-nm fluorescent nanospheres, or bovine immunoglobulin G (IgG) through layer-by-layer assembly by alternate adsorption with oppositely charged linear polyions. Sequential deposition on platelet surfaces of cationic poly(dimethyldiallylammonium chloride) and anionic poly(styrene sulfonate) was followed by adsorption of nanoparticles or immunoglobulins. Nanoorganized shells of platelets were demonstrated by transmission electron microscopy and fluorescence microscope images. Bovine IgG was assembled on platelets, as verified with anti-bovine IgG-FITC labeling. Localized targeting of anti-IgG shelled platelets was also demonstrated. An ability to coat blood cells with nano-organized shells can have applications in cardiovascular research and targeted drug delivery.
-
52浏览
-
0点赞
-
0收藏
-
0分享
-
78下载
-
0评论
-
引用
【期刊论文】N ano-encapsulation of furosemide microcrystals for controlled drug release
艾华, a a b Hua Ai, Steven A. Jones, Melgardt M. de Villiers, Yuri M. Lvova, *
Journal of Controlled Release 86(2003)59-68,-0001,():
-1年11月30日
Furosemide microcrystals were encapsulated with polyions and gelatin to control the release of the drug in aqueous solutions. Charged linear polyions and gelatin were alternatively deposited on 5-mm drug microcrystals through layer-bylayer (LbL) assembly. Sequential layers of poly(dimethyldiallyl ammonium chloride) (PDDA) and poly(styrenesulfonate) (PSS) were followed by adsorption of two to six gelatin /PSS bilayers with corresponding capsule wall thicknesses ranging from 45 to 115 nm. The release of furosemide from the coated microparticles was measured in aqueous solutions of pH 1.4 and 7.4. At both pH values, the release rate of furosemide from the encapsulated particles was reduced by 50-300 times (for capsules coated with two to six bilayers) compared to uncoated furosemide. The results provide a method of achieving prolonged drug release through self-assembly of polymeric shells on drug microcrystals.
Nano-encapsulation, Controlled release, Furosemide, Self-assembly
-
31浏览
-
0点赞
-
0收藏
-
0分享
-
583下载
-
0评论
-
引用
艾华, Xintao Shuaia, , Hua Aia, Norased Nasongklab, Saejeong Kima, Jinming Gaoa, *
Journal of Controlled Release 98(2004)415-426,-0001,():
-1年11月30日
Diblock copolymers of poly(q-caprolactone) (PCL) and monomethoxy poly(ethylene glycol) (MPEG) with various compositions were synthesized. The amphiphilic block copolymers self-assembled into nanoscopic micelles and their hydrophobic cores encapsulated doxorubicin (DOX) in aqueous solutions. The micelle diameter increased from 22.9 to 104.9 nm with the increasing PCL block length (2.5–24.7 kDa) in the copolymer composition. Hemolytic studies showed that free DOX caused 11% hemolysis at 200 Ag ml 1, while no hemolysis was detected with DOX-loaded micelles at the same drug concentration. An in vitro study at 37 JC demonstrated that DOX-release from micelles at pH 5.0 was much faster than that at pH 7.4. Confocal laser scanning microscopy (CLSM) demonstrated that DOX-loaded micelles accumulated mostly in cytoplasm instead of cell nuclei, in contrast to free DOX. Consistent with the in vitro release and CLSM results, a cytotoxicity study demonstrated that DOX-loaded micelles exhibited time-delayed cytotoxicity in human MCF-7 breast cancer cells. D 2004 Elsevier B.V. All rights reserved.
Polymer micelles, Drug delivery
-
36浏览
-
0点赞
-
0收藏
-
0分享
-
171下载
-
0评论
-
引用
【期刊论文】Electrostatic Layer-by-Layer Nanoassembly on Biological Microtemplates: Platelets
艾华, Hua Ai, † Ming Fang, ‡ Steven A. Jones, † and Yuri M. Lvov*, ‡
Biomacromolecules 2002, 3, 560-564,-0001,():
-1年11月30日
Platelets were coated with 78-nm silica nanoparticles, 45-nm fluorescent nanospheres, or bovine immunoglobulin G (IgG) through layer-by-layer assembly by alternate adsorption with oppositely charged linear polyions. Sequential deposition on platelet surfaces of cationic poly(dimethyldiallylammonium chloride) and anionic poly(styrene sulfonate) was followed by adsorption of nanoparticles or immunoglobulins. Nanoorganized shells of platelets were demonstrated by transmission electron microscopy and fluorescence microscope images. Bovine IgG was assembled on platelets, as verified with anti-bovine IgG-FITC labeling. Localized targeting of anti-IgG shelled platelets was also demonstrated. An ability to coat blood cells with nano-organized shells can have applications in cardiovascular research and targeted drug delivery.
-
50浏览
-
0点赞
-
0收藏
-
0分享
-
145下载
-
0评论
-
引用
【期刊论文】Coating and Selective Deposition of Nanofilm on Silicone Rubber for Cell Adhesion and Growth
艾华, Hua Ai, Yuri M. Lvov, David K. Mills, Merilyn Jennings, Jonathan S. Alexander, and Steven A. Jones, *
Cell Biochemistry and Biophysics,Volume 38, 2003,-0001,():
-1年11月30日
A recently developed method for surface modification, layer-by-layer (LbL) assembly, has been applied to silicone, and its ability to encourage endothelial cell growth and control cell growth patterns has been examined. The surfaces studied consisted of a precursor, with alternating cationic polyethyleneimine (PEI) and anionic sodium polystyrene sulfonate (PSS) layers followed by alternating gelatin and poly-D-lysine (PDL) layers. Film growth increased linearly with the number of layers. Each PSS/PEI bilayer was 3 nm thick, and each gelatin/PDL bilayer was 5 nm thick. All layers were more hydrophilic than the unmodified silicone rubber surface, as determined from contact angle measurements. The contact angle was primarily dictated by the outermost layer. Of the coatings studied, gelatin was the most hydrophilic. A film of (PSS/PEI)4/(gelatin/PDL)4/gelatin was highly favorable for cell adhesion and growth, in contrast to films of (PSS/PEI)8 or (PSS/PEI)8/PSS. Cell growth patterns were successfully controlled by selective deposition of microspheres on silicone rubber, using microcontact printing with a silicone stamp. Cell adhesion was confined to the region of microsphere deposition. These results demonstrate that the LbL selfassembly technique provides a general approach to coat and selectively deposit films with nanometer thickness on silicone rubber. Furthermore, they show that this method is a viable technique for controlling cellular adhesion and growth.
Nanofilm, micropatterning, layer-by-layer, silicone rubber, gelatin, poly-D-lysine.,
-
68浏览
-
0点赞
-
0收藏
-
0分享
-
283下载
-
0评论
-
引用
艾华, Xintao Shuai, Marty D. Pagel, David Farrell, Jeffey, Duerk, mad Jinming Gao*
Adv. Mater. 2005, 17, 1949-1952,-0001,():
-1年11月30日
-
53浏览
-
0点赞
-
0收藏
-
0分享
-
205下载
-
0评论
-
引用
【期刊论文】Interactions between self-assembled polyelectrolyte shells and tumor cells
艾华, Hua Ai, John J. Pink, Xintao Shuai, David A. Boothman, Jinming Gao
,-0001,():
-1年11月30日
Layer-by-layer self-assembled polyelectrolyte shells are a new class of micro/nanocapsules with unique physicochemical properties for potential applications in drug/gene delivery. The objective of this study was to investigate the interactions of polyelectrolyte shells (1 min diameter) with MCF-7 breast cancer cells and identify key parameters that affect such interactions. Tailoring of surface properties of polyelectrolyte shells was achieved by choosing different outermost layer materials, including cationic polymers, anionic polymers, and lipid bilayers. Different surface compositions led to a wide range of electrostatic potentials from 46 to 47mv in phophate-buffered saline buffer. Confocal microscopy studies showed that the polyelectrolyte shells were internalized into the cell cytoplasm, but not into the nuclei. Correlation of cell uptake with shell surface compositions was complicated by the adsorption of serum proteins on the surface of polyelectrolyte shells, particularly polycation-coated shells. To prevent protein adsorption, poly(ethylene glycol) (PEG) grafted poly(ethyleneimine) (PEI) copolymers (1:1, 1:5, 1:10 graft ratios) were synthesized and introduced on the shell surface. Shells coated with PEI-PEG copolymers effectively reduced protein adsorption whereas PEI-PEG copolymers with lower graft ratios achieved higher cell uptake efficiency after 24 h of incubation with MCF-7 cells.
layer-by-layer self-assembly, polyelectrolyte shells, poly (, ethyleneimine), (, PEI), -poly (, ethylene glycol), (, PEG), , shell-cell interactions
-
47浏览
-
0点赞
-
0收藏
-
0分享
-
102下载
-
0评论
-
引用