郜洪文
水污染控制与资源化、环境毒理、现场环境检测等。
个性化签名
- 姓名:郜洪文
- 目前身份:
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学术头衔:
博士生导师
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学科领域:
环境科学技术基础学科
- 研究兴趣:水污染控制与资源化、环境毒理、现场环境检测等。
安徽淮北人。研究员,博士生导师,享受国务院特殊津贴。同济大学污染控制与资源化研究国家重点实验室-环境分子科学研究室 (Tongji - EMSL)学术带头人。研究方向:水污染控制与资源化、环境毒理、现场环境检测等。承担国家自然科学基金、国家科技支撑计划、973、国家重大科技专项、上海市重点基础研究等科研项目,创立Spectral Correction Technique、Light-Absorption Ratio Variation、Transmembrane Impedance Effect、In-situ Waste hybridization Treatment等,在Proteomics、Biophys. J.、J. Mater. Chem.、Toxicol. Sci.、Amino Acids、Analyst、Water Res.、BMC Mol. Biol.等国际主流学术期刊发表一百余篇论文。获省部级科技奖四项、国家发明专利二十项、计算机软件著作权四项。1999年获中国化学会青年化学奖, 2003年入选ISI - Emerging Research Fronts。
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20
【期刊论文】Comparative Transmembrane Transports of Three Typical Lipophilic Organic Chemicals
郜洪文, J. R. Ren, H. P. Zhao, C. Song, S. L Wang, L. Li, Y. T. Xu and H. W. Gao
Biores. Technol., 2010, 101(22):8632-8638 ,-0001,():
-1年11月30日
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【期刊论文】Transmembrane distribution of kanamycin and chloramphenicol:
郜洪文, Chao Song, Nai-Yun Gao and Hong-Wen Gao
Mol. BioSyst., 2010, 6(10):1901-1910 ,-0001,():
-1年11月30日
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郜洪文, Dan-Hua Zhao, Ya-Lei Zhang, Yan-Ping Wei and Hong-Wen Gao*
J. Mater. Chem. 2009, 19, 7239-7244,-0001,():
-1年11月30日
Inorganic/organic hybridization is often employed to synthesize unctional materials but seldom considered in industrial wastewater treatment. Dye conjugate hybridization was proposed by immobilizing the anionic (e.g. congo red)-cationic (e.g. methylene blue) dye complex with growing calcium carbonate. The 3D-morphology and structure of the conjugate hybrid were characterized and the mechanism of formation elucidated. The shell-core structural hybrid was formed and then stacked into cubes. Applied to treatment of concentrated organic wastewaters, the simple single-step hybridization performed adsorption, flocculation and ionic complexation and exhibited a high level of removal of organic substances. This work provides a convenient, cost-effective and environmentfriendly wastewater treatment by "using waste to treat waste".
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郜洪文, Xian Zhang, Ling Chen, Xun-Chang Fei, Yin-Sheng Ma and Hong-Wen Gao*
BMC Molecular Biology 2009, 10: 16,-0001,():
-1年11月30日
Background: Health risk from exposure of perfluorochemicals (PFCs) to wildlife and human has been a subject of great interest for understanding their molecular mechanism of toxicity. Although much work has been done, the toxigenicity of PFCs remains largely unknown. In this work, the noncovalent interactions between perfluorooctane sulfonate (PFOS) and serum albumin (SA) and DNA were investigated under normal physiological conditions, aiming to elucidate the toxigenicity of PFCs. Results: In equilibrium dialysis assay, the bindings of PFOS to SA correspond to the Langmuir isothermal model with two-step sequence model. The saturation binding number of PFOS was 45 per molecule of SA and 1 per three base-pairs of DNA, respectively. ITC results showed that all the interactions were spontaneous driven by entropy change. Static quenching of the fluorescence of SA was observed when interacting with PFOS, indicating PFOS bound Trp residue of SA. CD spectra of SA and DNA changed obviously in the presence of PFOS. At normal physiological conditions, 1.2 mmol/l PFOS reduces the binding ratio of Vitamin B2 to SA by more than 30%. Conclusion: The ion bond, van der Waals force and hydrophobic interaction contributed to PFOS binding to peptide chain of SA and to the groove bases of DNA duplex. The non-covalent interactions of PFOS with SA and DNA alter their secondary conformations, with the physiological function of SA to transport Vitamin B2 being inhibited consequently. This work provides a useful experimental method for further studying the toxigenicity of PFCs.
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郜洪文, Fang-Fang Chen, Yi-Nan Tang, Shi-Long Wang, Hong-Wen Gao
Amino Acids (2009) 36: 399-407,-0001,():
-1年11月30日
The non-covalent interaction of brilliant red (BR) with lysozyme was investigated by the UV spectrometry, circular dichroism (CD) and isothermal titration calorimetry (ITC). The thermodynamic characterization of the interaction was performed and the assembly complexes were formed: lysozyme (BR) 17 at pH 2.03, lysozyme (BR) 15 at pH 3.25 and lysozyme (BR) 12 at pH 4.35, which corresponded to the physiological acidities. The ionic interaction induces a combination of multiple non-covalent bonds including hydrogen bond, hydrophobic interaction and van der Waals force. The two-step binding model of BR was found, in which one or two BR molecules entered the hydrophobic intracavity of lysozyme and the others bound to the hydrophilic outer surface of lysozyme. Moreover, BR binding resulted in change of the lysozyme conformation and inhibition of the lysozyme activity. The possible binding site and type of BR and the conformational transition of lysozyme were speculated and illustrated. This work provided a useful approach for study on enzyme toxicity of aromatic azo chemicals.
Aromatic azo compound, Lysozyme, Non-covalent binding, Enzyme toxicity
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郜洪文, Zhen Xu a, Shi-Long Wang b, Hong-Wen Gao a, ∗
Journal of Hazardous Materials 180 (2010) 375-383,-0001,():
-1年11月30日
Nanomaterials are finding increasing use in industrial production and daily life. However, human exposure to them may cause health risks. Nano-SiO2 was selected as a representative nanomaterial and its potential effects were investigated in terms of its interactions with cytochrome c (cyt c), deoxyribonuclease (DNase Ⅱ) and hemoglobin (Hb). The interactions accorded with Langmuir isothermal adsorption; the saturation binding numbers for cyt c, DNase Ⅱ and Hb were 42±5, 24±2 and 1.1±0.1 mol/g nano-SiO2 particle at pH 7.4, respectively, and the corresponding stability constants were 6.15×105, 1.79×106 and 2.6×107M−1. on the basis of the binding constants and of -potential fluorescence and circular dichroism (CD) measurements and scanning electronic microscopy (SEM), it was found that the three functional proteins can bridge nano-SiO2 particles via charge attraction and hydrogen bonding and aggregate them into coralloid forms. The interactions also changed the secondary structures of the proteins and inhibited their static and dynamic activities. It may reasonably be deduced that exposure to nano-size silicon dioxide particles e.g. as drug carriers may have an unfavorable effect on human health by inactivating functional proteins.
Silica, Protein, Nanoparticles, Surface adsorption, Molecular interaction
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郜洪文, Dan-Hua Zhao, a Yu-Lin Shen, a Ya-Lei Zhang, *b De-Quan Wei, a Nai-Yun Gao a and Hong-Wen Gao*a
J. Mater. Chem. 2010, 20, 3098-3016,-0001,():
-1年11月30日
A new kind of milli-sized sorbent-calcium alginate supporting a weak acidic pink red B (APRB)-calcium fluoride nano-sized hybrid-was prepared. The micro-structure of the hybrid and adsorbent were characterized, and its adsorption capacity for organic contaminants, e.g. dyes, PCBs and microcystin (MC)-LR, was investigated. Cationic dyes were adsorbed selectively via charge attraction, with a saturation capacity of 188 mg of cationic red 3R per gram of the milli-sized adsorbent. However, the adsorptions of PCBs and MC-LR corresponded to the octanol-water partition law, and their partition coefficients were calculated to be 7788.6mgkg 1 for PCB029, 13325mgkg 1 for PCB101, 434.6mgkg 1 for PCB180, and 369.2 mg kg 1 for MC-LR. When the milli-sized adsorbent was used in the treatment of two concentrated dye wastewaters and two polluted ground waters, the removal of the colored substances, chemical oxygen demand (COD), and concentration of P Bs and MC-LR were satisfactory. In the synthesis of the materials, all of the reactants are easily available and harmless to the environment, and APRB may be obtained from concentrated APRB-containing wastewater instead. The development of the milli-sized hybrid adsorbent provided liquid-solid separation, for favorable use in engineering. It will play an important role in the treatment of concentrated wastewater and the remediation of ground water contamination.
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【期刊论文】Potential Protein Toxicity of Synthetic Pigments: Binding of Poncean Sto Human Serum Albumin
郜洪文, Hong-Wen Gao, Qing Xu, Ling Chen, Shi-Long Wang, Yuan Wang, Ling-Ling Wu, and Yuan Yuan
Biophysical Journal Volume 94 February 2008 906-917,-0001,():
-1年11月30日
Using various methods, e.g., spectrophotometry, circular dichroism, and isothermal titration calorimetry, the interaction of poncean S (PS) with human serum albumin (HSA) was characterized at pH 1.81, 3.56, and 7.40 using the spectral correction technique, and Langmuir and Temkin isothermal models. The consistency among results concerning, e.g., binding number, binding energy, and type of binding, showed that ion pair electrostatic attraction fixed the position of PS in HSA and subsequently induced a combination of multiple noncovalent bonds such as H-bonds, hydrophobic interactions, and van der Waals forces. Ion pair attraction and H-bonds produced a stable PS-HSA complex and led to a marked change in the secondary structure of HSA in acidic media. The PS-HSA binding pattern and the process of change in HSA conformation were also investigated. The potentially toxic effect of PS on the transport function of HSA in a normal physiological environment was analyzed. This work provides a useful experimental strategy for studying the interaction of organic substances with biomacromolecules, helping us to understand the activity or mechanism of toxicity of an organic compound.
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郜洪文, Hong-Wen Gao, Jian-Fu Zhao, Qiao-Zhi Yang, Xiang-Hu Liu, Ling Chen and Lu-Ting Pan
Proteomics 2006, 6, 5140-5151,-0001,():
-1年11月30日
The interactions of 2', 4', 5', 7'-tetrabromo-4, 5, 6, 7-tetrachlorofluorescein (TBTCF) with BSA, ovalbumin (OVA) and poly-L-lysine (PLYS) at pH 3.70 have been investigated by combination of the spectral correction technique and the Langmuir isothermal adsorption. The active connection actions such as ion pairs, van der Waals' force, hydrogen bond, hydrophobic bond were proposed to explain the non-covalent interaction between TBTCF and BSA, OVA and PLYS. Effects of the electrolyte and high temperature indicated that union of the active connections between TBTCF and BSA and OVA was too firm to be destroyed. The relationship between the binding number of TBTCF and variety fraction of the amino acid residues was analyzed. The binding number of TBTCF depended on the number of positively charged amino acid residues. The other amino acid residues surrounded and seized TBTCF by hydrogen bonds and hydrophobic bonds when the electrostatic attraction pulled TBTCF to link protein. In addition, a novel method named the absorbance ratio difference was established for determination of protein in trace level and was applied with much higher sensitivity than the ordinary method.
Absorbance ratio difference, Langmuir isothermal adsorption, Non-covalent interaction, Spectral correction technique, 2', ,, 4', ,, 5', ,, 7', -Tetrabromo-4,, 5,, 6,, 7-tetrachloro-fluorescein
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郜洪文, Hong-Wen Gao a, *, Si-Qing Xia a, Hong-Yan Wang b, Jian-Fu Zhao a
Water Research 38(2004)1642-1650,-0001,():
-1年11月30日
The light-absorption ratio variation approach (LARVA) has been described and applied to the quantitative detection ofultramicro amounts of Ni by spectrophotometry, which raises notably the detection sensitivity. The complexation between 1, 5-di (2-hydroxy-5-sulfophenyl)-3-cyanoformazan (DSPCF) and Ni (II) at pH 9.11 was investigated and the binary complex was characterized by the spectral correction technique. Results have shown that DA 1r (DAr-lightabsorption ratio variation) is linear in the range ofNi (II) between 5 and 200ng=ml: The limit ofdetection
Light-absorption ratio variation approach, Spectrophotometry, 1,, 5-di (, 2-hydroxy-5-sulfophenyl), -3-cyanoformazan, Characterization ofcomplex, Determination of Ni in ultramicro level.,
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