李旭东
博士 教授 博士生导师
四川大学 生物材料中心
组织修复与再生;生物材料;药物控释;结晶学工程;植物多酚;胶原;多糖;儿茶素
趋势在经意或浑然间形成,上势盎然,下势徒劳;然,势含转变,故,蓄势之际,亦意在长远。
- 姓名:李旭东
- 目前身份:在职研究人员
- 担任导师情况:博士生导师
- 学位:博士
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学术头衔:
博士生导师
- 职称:高级-教授
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学科领域:
生物医学工程学
- 研究兴趣:组织修复与再生;生物材料;药物控释;结晶学工程;植物多酚;胶原;多糖;儿茶素
李旭东,男,1964年2月生,四川大学教授,博士生导师,英国伦敦大学玛丽皇后学院博士,英国期刊《Advances in Applied Ceramics》(原《British Ceramic Transactions》)和《生物医学工程学杂志》编委。长期从事组织修复材料的基础研究、产品开发及应用工作,研究领域先后涉及磷酸钙盐生物活性陶瓷、表面活性涂层(薄膜)、医用复合材料(辅料)、金属有机陶瓷前驱体、纳米生物材料等,参研“等离子喷涂羟基磷灰石涂层-钛基牙种植体”项目1995年获国家教委科技进步一等奖(第6完成人)。2000年获英国国家材料所(IOM3)Andrew Carnegie基金会资助在英国学习,2004年四川大学引进人才。 作为负责人先后承担973课题、国家自然科学基金面上项目、教育部博士点基金、四川省重点科攻关项目等,参研多个国家级项目等,致力于医用复合材料(敷料)、纳米生物材料、主客体载体、结晶学工程和植物多酚等方面的研究工作。 在组织修复再生材料研究上,通过组成和结构仿生,实现获得基于胶原的致密、多孔和膜状类骨纳米复合材料(授权专利名:有机-无机复合生物材料及其制备方法,ZL200610020802.4),并建立基于胶原、人源头发角质蛋白和多糖的多种组织修复材料或辅料;有关结晶学的研究涉及通过多羟基化合物调控磷酸钙、碳酸钙和草酸钙这三类重要的生物无机钙盐,已取得的工作入选Nature China (自然中国)突出研究成果,入选ACS Editors' Choice(编辑良择2020.6.22);构建出简单、高产率、分散稳定的纳米磷酸钙合成技术(发明专利,专利号ZL201010206973.2,200910058587.0和200910059289.3);通过氧化偶联聚合获得绿茶多酚功能球,启动了植物多酚在生物材料上的应用。研究论文以第一或通讯作者发表在JACS, Chem Mater, Chem Comm、Biomaterials、ACS Appl Mater Interface、ACS Sustainable Chem Eng等20多种SCI源刊上。申请发明专利10项(已授权7项)。 研究方向:胶原-角质蛋白-多糖组织工程材料、药物控释载体和茶(植物)多酚的生物医学功效。 https://xli20004.github.io/
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4832
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成果阅读
1175
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成果数
13
李旭东
ACS Biomaterials Science & Engineering,2020,6(6):3275-3286
2020年04月28日
The understanding of the mineralization of collagen for bone formation is a current key theme in bone tissue engineering and is of great relevance to the fabrication of novel biomimetic bone grafting materials. The noncollagenous proteins (NCPs) play a vital role in bone formation and are considered to be responsible for regulating intrafibrillar penetration of minerals into collagen fibrils by means of their abundant polyanionic domains. In this study, alginate, as a NCPs analogue, was introduced in the mineralization of collagen to mediate the collagen self-assembly with simultaneous hydroxyapatite (HA) synthesis. The biomimetic systems were based upon the self-assembly of collagen (Col) or collagen-alginate (CA) in the absence or presence of a varying content of HA. The alginate-mediated effects were found to include the lateral aggregation of small fibrils into the extremely large bundles and the assisted deposition of HA for a larger mineralized fibril. This alginate-assisted mineralization of collagen gave rise to an exquisite 3D mineralized architecture with enhanced mechanical property. The cell viability experiments showed the excellent proliferation and spreading morphologies of rat bone mesenchymal stem cells (MSCs) on the assembled products, and a higher expression of osteogenic differentiation related transcription factor was obtained in the alginate-assisted mineralization of collagen. This study indicated that the selection of an appropriate substance, e.g., alginate as an anionic polyelectrolyte with Ca-capturing property, could be a convenient, simple solution to achieve a mineralized collagen scaffold with the reinforced mechanical property for potential applications in bone regeneration.
Collagen, Self assembly, Alginate
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【期刊论文】Modular Assembly of Versatile Nanoparticles with Epigallocatechin Gallate
李旭东
ACS Sustainable Chem. Eng.,2020,8(26):9833–9845
2020年06月03日
Various nanotechnologies have been extensively developed to prepare nanoparticles with different features for satisfying the requirements of diverse fields, but the current achievements are confined to different material systems for the limited acquisition with desirable properties. Here, we demonstrated a flexible strategy based on the broad selectivity of amines in the condensation of green tea polyphenol-EGCG (epigallocatechin gallate), formaldehyde, and amines for the rational design and preparation of versatile nanomaterials. With EGCG as the sole material system and amines (R–NH2) of various functional R groups as the selectable modules, the modular assembly of polyphenol-activated condensation was straightforward and completed in one step, giving rise to different polyphenolic nanoparticles variable in surface chemistry (−amine, −aldehyde, and −carboxyl), shapes (sphere, dumbbell, walnut), internal structures (solid, hollow, and porous), stimuli responsiveness (-s-s-), and fluorescence. The flexibility of the polyphenolic condensation for versatile nanoparticles was further demonstrated by the incorporation of amino-containing anticancer or antibacterial drugs into polyphenolic nanoparticles as nanodrugs. The present study totally involved the use of 13 different amines to synthesize 18 different nanoparticles, not only convincingly specifying the enormous value of the polyphenolic condensation as platform for modular assembly of versatile nanoparticles but also revolutionizing the current strategies and methodologies for encapsulated applications of tea polyphenols.
Tea polyphenol, Platform
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Xudong Li
REGENERATIVE BIOMATERIALS,2018,5(2):93-103
2018年03月07日
Collagen (COL), collagen/hydroxyapatite (COL/HA), HA and biphasic calcium phosphate were prepared as representative bone grafting materials with composition analogous to bone, and their structural characteristics were analyzed. The rat bone mesenchymal stem cells (BMSCs) were further seeded onto four groups of materials, and BMSCs grown in basic medium and standard osteogenic medium were set as controls of a reference model to show the basic and osteogenic behavior of cells without the intervention of materials. Cellular behaviors were characterized, including proliferation, spreading morphology and expression of osteogenesis factors. The rat BMSCs proliferated properly with time on four groups of materials as well on two groups of controls, and typical cuboidal, polygonal and extremely-elongated morphologies of cells were observed. According to the real-time polymerase chain reaction data, a higher osteogenic gene expression level was dependent upon the growing morphology but not the proliferation rate of cells, and the osteogenic differentiation capacity of cells onto four groups of materials varied in specific genes. In general, BMSCs exhibited the highest osteogenic capacity onto COL/HA, but the poorest onto HA. The growing behaviors of cells on materials were further discussed in comparison with the cases of OC and BC of the reference model. The present attempt to comparatively analyze cell experimental data with a reference model is expected to be useful for revealing the difference in the osteogenic capability of MSCs onto materials or even the bioactivity of materials.
osteogenic differentiation, spreading morphology, mineralization of collagen, bioceramics
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Xudong Li
Journal of the American Chemical Society,2013,135(11):4179-4182
2013年03月07日
Green luminescent, monodisperse, smooth, porous and hollow spheres were simply prepared by Cu2+ and temperature mediated oxidative coupling assembly of green tea polyphenols in water. These polymeric tea polyphenol spheres are GSH responsive, acid resistant but alkali-responsive, ideally used as platform for controlled delivery of functional guests.
DRUG-DELIVERY, POLYMERIZATION, ANTIOXIDANT, FLAVONOIDS
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Xudong Li
Journal of Materials Chemistry B,2018,6(9):1373-1386
2018年01月31日
While intelligent nanoparticles with therapeutic effects provide a resolving strategy for low drug loading efficacy, poor metabolism and elimination of current nanoparticulate drug delivery systems, precise preparation of colloidally stable but stimuli-responsive nanocarriers with size tunability is still a challenging task. Here, we develop a facile and sustainable method through the use of naturally reproducible green tea polyphenols and hair keratins to prepare biocompatible, colloidally stable, stimuli-responsive nanoparticles with therapeutic effects. The present strategy simply involves covalent interactions of tea catechins and keratins, giving rise to the molecular assembly of size-controlled nanoparticles (30–230 nm) which are long-term colloidally stable at physiological media but are disassembled under pathological conditions, ideally for targeted delivery of anticancer drugs. The cell experiments confirmed that these nanoparticles are bio-safe, have the inherent bioactivity of tea catechins, and that the drug-loaded nanoparticles yield a higher cancer cell inhibition rate than free drugs. In addition, the nanoparticles are found to improve the bioavailability of tea polyphenols, according to animal studies, which further demonstrates that the use of nanoparticles as drug carriers results in enhanced anticancer efficacy with negligible systemic toxicity. Given that large-scale preparation of size-controlled nanoparticles could already be easily achieved, the present study actually provides an innovative nanotechnological approach to make good use of green tea polyphenols with beneficial health effects, potentially for therapeutic and preventive purposes.
DRUG-DELIVERY, CATECHIN DERIVATIVES, NANOTECHNOLOGY
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李旭东, Xudong Li*, Xiaomin Wang, Xiaoyan Lin, Jianming Jiang, Xingdong Zhang
Key Engineering Materials Vols. 309-311,-0001,():
-1年11月30日
An organic/inorganic composite hydrogel route was used to prepare collagen-calcium phosphate hybrids with high mechanical strengths, via in-situ mineral synthesis during collagen fibrillogenesis followed by dehydration. An array of characterization techniques including X-ray diffraction and Fourier transform infrared spectroscopy analyses confirmed that the final products are analogous to natural bone. A three-point bending strength of 70 MPa, much higher than the values reported in the literature, was recorded in the present case, due to the three dimensional network structure achieved between inorganic and organic phases. This innovative method provides an efficient route to produce bone grafts with the desirable mechanical properties which are dependent upon the actual inorganic/organic ratio and water content.
Engineering Research Center in Biomaterials,, Sichuan University,, Chengdu 610064,, PR China
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李旭东, X. Y. Lin , , H. S. Fan, X. D. Lia, M. Tang and X. D. Zhang
Key Engineering Materials Vols. 284-286,-0001,():
-1年11月30日
A nano-hydroxyapatie/collagen composite was fabricated by an in situ synthesis technique. Transmission electron microscopy (TEM) and selected area electron diffraction (SAED) analyses for the composite indicated crystals on the collagen fibril matrix exhibited certain orientation. Bioactivity of the composite was investigated through in vitro tests in a sterile simulated body fluid (SBF) system. Scanning electron microscopy and energy-dispersive X-ray analysis of the composite showed that the composite had the ability to induce the formation of calcium phosphate crystals on the surface of the composite in SBF. The cytocompatibility of the composite was evaluated by in vitro cytotoxicity test. Both the results from MTT assay and scanning electron microscope (SEM) observations indicated that the composite had no adverse impact on cell proliferation and morphology. The results imply the composite is high bioactive and has good cytocompatibility
nanohydroxyapatite, collagen, composite, bioactivity, cytocompatibility
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【期刊论文】Preparation and Characterization of Collagen by Hydrogel Formation Method
李旭东, Jian Lua, Xiaoyan Lin, Bo Jiang, Xudong Li*, b, Jiyong Chen, Xingdong Zhang
Key Engineering Materials Vols. 288-289,-0001,():
-1年11月30日
A kind of medical collagen was prepared by hydrogel formation method. Chemical and physical properties were investigated by FTIR, amino acid analysis, SDS-PAGE, carbohydrate content analysis, heavy metal content analysis. Degradation experiments in vivo and subsequent histological investigations were carried out to evaluate the biological performance. The results suggested that the collagen achieved is promising in tissue engineering scaffold materials for a long-term (more than 12 weeks) implantation application.
collagen,, hydrogel,, degradation
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【期刊论文】In situ synthesis of bone-like apatite/collagen nano-composite at low temperature
李旭东, Xiaoyan Lin a, b, Xudong Li a, *, Hongsong Fan a, Xiantao Wen a, Jian Lu a, Xingdong Zhang a
Materials Letters 3570(2004)3569-3572,-0001,():
-1年11月30日
A nano-composite of bone-like apatite/collagen was prepared by a new method-low-temperature in situ synthesis using calcium nitrate, diammoniun hydrogen phosphate and cow hide collagen as starting materials. The composite was investigated via X-ray diffraction (XRD), Fourier transform infrared (FTIR), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). It was found that bone-like nanoapatite particles were distributed uniformly in collagen fibrils in the composite. The composite with homogeneous microstructure was similar to natural bone in crystallite phase composition and crystal size. The biomimetic composite is expected to exhibit desirable properties in biomedical applications.
Nanocomposite, Bone-like apatite, Collagen, Low temperature, In situ synthesis
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【期刊论文】In Situ Synthesis of PVA-PAA-HA Interpenetrating Composite Hydrogel
李旭东, Xiaomin Wang, , Xudong Li*, Guiqiu Zheng, Jianming Jiang, Jiyong Chen, Xingdong Zhang
Key Engineering Materials Vols. 309-311,-0001,():
-1年11月30日
A composite hydrogel with interpenetrating network structure was prepared via in-situ synthesis of calcium phosphates during the physical-chemical crosslinking of poly (vinyl alcohol) (PVA) and poly (acrylic acid) (PAA). The hydrogel water content was tested. Fourier transform infrared absorption spectrum (FT-IR), X-ray diffraction and scanning electron microscopy were employed to evaluate the characteristics of the composite hydrogel. The results showed that the composite hydrogel had high water content and that the inorganic phase was poorly crystalline calcium phosphates. FT-IR confirmed that the interpenetrating network structure was formed between PVA and PAA. The chemical interactions between inorganic and organic phases were further investigated and discussed. The composite hydrogel with an interpenetrating network achieved using the present novel method could be a promising material for tissue engineering.
composite hydrogel,, interpenetrating network (, IPN), ,, poly (, vinyl alcohol), ,, poly (, acrylic acid), ,, hydroxyapatite,, in-situ synthesis
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