刘锋
生化与药物分析 1.石英晶体微天平生物传感器的理论及其应用 2.仿生识别材料的合成、性质及其应用 3.新型双水相体系的理论及其应用
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- 姓名:刘锋
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学术头衔:
博士生导师
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学科领域:
分析化学
- 研究兴趣:生化与药物分析 1.石英晶体微天平生物传感器的理论及其应用 2.仿生识别材料的合成、性质及其应用 3.新型双水相体系的理论及其应用
研究领域:生化与药物分析 1.石英晶体微天平生物传感器的理论及其应用 2.仿生识别材料的合成、性质及其应用 3.新型双水相体系的理论及其应用
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424
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成果数
8
刘锋, Chiyang He, Feng Liu, Kean Li, and Huwei Liu
Analytical Letters, 39: 275-286, 2006,-0001,():
-1年11月30日
A type of testosterone-imprinted polymer film grafted from porous silica was prepared by covalently binding azo-initiators and then photo-grafting. Elemental analysis and infrared (IR) spectroscopy attested the polymer formation, The material could be polymerized within 10-60min with reproducible grafting kinetics, controlla-ble film thickness, and obviously specific recognition ability to testosterone with the imprinting factor of i.52. Due to its uniform size, spherical shape, controllable film thickness, and accessible sites near or at the surface, this polymer could serve as a sensing element, solid-phase extraction material, or chromatographic stationary phase to selectively recognize or separate testosterone.
Surface grafting,, molecularly imprinted polymer,, porous silica,, testosterone
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【期刊论文】Extraction of testosterone and epitestosterone in human urine using 2-propanol-salt-H2O system
刘锋, Hailing Liua, b, Chiyang Hea, Dawei Wena, Huwei Liua, Feng Liua, ∗, Kean Lia
Analytica Chimica Acta 557(2006)329-336,-0001,():
-1年11月30日
Based on the 2-propanol-salt-H2O system, a new and simple pretreatment method was developed for the analysis of testosterone and epitestosterone in human urine coupled with high-performance liquid chromatography (HPLC). The influence factors on partition behaviors of the steroids were studied including the type and amount of salts, the volume of 2-propanol, the concentration of the analytes, and temperature. When K3PO4, K2HPO4, K2CO3 and KOH were chosen as phase separation salts, the enrichment factors were 12, 10, 12 and 13, respectively, and the extraction efficiencies for testosterone and epitestosterone were all 80-90%. In the optimal system of 2-propanol-K3PO4-H2O, the recoveries of the spiked standards for the analytes were all 92-102% with relative standard deviation of 2.3-7.2%. Combined with a HPLC method, this extraction technique has been successfully applied to the analysis of testosterone and epitestosterone in human urine with the detection limits of 1 ng/ml and the linear ranges of 10-500 ng/ml for both compounds. Compared with conventional liquid-liquid extraction or solid-phase extraction, this novel method is much simpler and more environmentally friendly due to the direct injection of the upper phase into HPLC system, no use of harmful organic solvent, and low consumption of extractant.
Extraction, Testosterone, Epitestosterone, Aqueous two-phase system, 2-Propanol, High-performance liquid chromatography
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刘锋, Shehong Li, Chiyang He, Huwei Liu, Kean Li, Feng Liu∗
J. Chromatogr. B 826(2005)58-62,-0001,():
-1年11月30日
An ionic liquid, 1-butyl-3-methylimidazolium chloride ([C4mim]Cl)/salt aqueous two-phase systems (ATPS) was presented as a simple, rapid and effective sample pretreatment technique coupled with high-performance liquid chromatography (HPLC) for analysis of the major opium alkaloids in Pericarpium papaveris. To find optimal conditions, the partition behaviors of codeine and papaverine in ionic liquid/salt aqueous two-phase systems were investigated. Various factors were considered systematically, and the results indicated that both the pH value and the salting-out ability of salt had great influence on phase separation. The recoveries of codeine and papaverine were 90.0-100.2% and 99.3-102.0%, respectively, from aqueous samples of P. papaveris by the proposed method.
Aqueous two-phase systems, Ionic liquid, Extraction, Opium alkaloids
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刘锋, Chiyang He, Shehong Li, Huwei Liu, Kean Li, Feng Liu∗
J. Chromatogr. A 1082(2005)143-149,-0001,():
-1年11月30日
Based on aqueous two-phase systems (ATPS) consisting of 1-butyl-3-methylimidazolium chloride, a hydrophilic ionic liquid (IL), and K2HPO4, a new and simple extraction technique, coupled with a reversed-phase high performance liquid chromatography (RP-HPLC), was developed for the simultaneous concentration and analysis of testosterone (T) and epitestosterone (ET) in human urine. Under the optimal conditions, the extraction efficiencies for both analytes were 80-90% in a one-step extraction. The method required only 3.0mL of urine and a single hydrolysis/deproteinization/extraction step followed by direct injection of the IL-rich upper phase into HPLC system for analysis. The method has been satisfactorily applied to the analysis of T and ET in human urine with detection limits of 1 ng/mL and linear ranges of 10-500ng/mL for both compounds. Compared with conventional liquid-liquid extraction or solid phase extraction, this new method is much "greener" due to no use of volatile organic solvent and low consumption of IL. The proposed extraction technique opens up new possibilities in the separation of other drugs.
Extraction, Ionic liquid, Aqueous two-phase systems, Testosterone, Epitestosterone, Human urine
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【期刊论文】Selection of Ligands for Affinity Chromatography Using Quartz Crystal Biosensor
刘锋, Yang Liu, †, Xiaoling Tang, ‡, Feng Liu, and Ke¢an Li*
Anal. Chem. 2005, 77, 4248-4256,-0001,():
-1年11月30日
This paper described a new strategy for rapid selecting ligands for application in affinity chromatography using a quartz crystal microbalance (QCM) biosensor. An aminoglycoside antibiotic drug, kanamycin (KM), was immobilized on the gold electrodes of the QCM sensor chip. The binding interactions of the immobilized KM with various proteins in solution were monitored as the variations of the resonant frequency of the modified sensor. Such a rapid screen analysis of interactions indicated clearly that KM-immobilized sensor showed strong specific interaction only with lysozyme (LZM). The resultant sensorgrams were rapidly analyzed by using a kinetic analysis software based on a genetic algorithm to derive both the kinetic rate constants (kass and kdiss) and equilibrium dissociation constants (KD) for LZM-KM interactions. The immobilized KM showed higher affinity to LZM with a dissociation constant on the order of 10-5M, which is within the range of 10-4-10-8M and suitable for an affinity ligand. Therefore, KM was demonstrated for the first time as a novel affinity ligand for purification of LZM and immobilized onto the epoxy-activated silica in the presence of a high potassium phosphate concentration. The KM immobilized affinity column has proved useful for a very convenient purification of LZM from chicken egg white. The purity of LZM obtained was higher than 90%, as determined by densitometric scanninng of sodium dodecyl sulfate-polyacrylamide gel electrophoresis of purified fraction. These results confirmed that the selected KM ligand is indeed a valuable affinity ligand for purification of LZM. The new screening strategy based on a QCM biosensor is expected to be a promising way for rapid selecting specific ligands for purifying other valuable proteins.
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刘锋, Lei Li, Feng Liu∗, Xiangxu Kong, Shun Su, Ke An Li
Analytica Chimica Acta 452(2002)321-328,-0001,():
-1年11月30日
Based on the investigation of a non-ionic surfactant-salt-H2O liquid-liquid extraction system, general rules concerning salt selection are concluded and the mechanism of phase separation is explained. The extraction behavior of chlorpromazine hydrochloride (CPZ) and procaine hydrochloride (PCN) in such a system is studied. Research shows that the extraction efficiency of CPZ can amount to 96% by twice extraction, which means that quantitative extraction is realized, while that of PCN is 77%. This system produces distribution coefficients (KD) of 12.3 and 2.6, respectively, for CPZ and PCN. Extraction mechanism is deduced according to ultraviolet absorbance; and molecular fluorescence spectra change of the drugs in the system studied.
Non-ionic surfactant-salt-H2O extraction system, Polyethylene glycol-1000, Chlorpromazine hydrochloride, Procaine hydrochloride, Mechanism of extraction
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刘锋, Tieli Zhang, Feng Liu∗, Wen Chen, Jun Wang, Kean Li
Analytica Chimica Acta 450(2001)53-61,-0001,():
-1年11月30日
Three molecularly imprinted polymers (MIPs) were prepared corresponding to three structurally related template compounds 4-hydroxybenzoic acid (4-HBA), gentisic acid (GA) and salicylic acid (SA) that differ in intramolecular hydrogen bonding ability using acrylamide (AA) as a functional monomer. HPLC methodwas used to evaluate the binding performances of the MIPs to the templates and several analogues. The results showed that the difference in their molecular recognition ability was pronounced. The highest molecular recognition ability was observed for 4-HBA-imprinted polymer. It was proved that the hydrogen bond interaction between the functional monomer and the template (4-HBA) played a major role in the recognition process and Scatchard analysis showed that two classes of binding sites were formed in 4-HBA-imprinted polymer. Their dissociation constants were estimated to be 1.76×10−4 and 1.40×10−3 mol l−1, respectively. But for GA-or SA-imprinted polymer the molecular recognition ability was not improved compared to the blank polymer (BP). By comparison of the structures of the three templates, it was concluded that the molecular recognition ability will decrease when the template itself is able to form intramolecular hydrogen bond in the molecular imprinting process. This study will be helpful for us to understand the molecular recognition mechanism of MIPs and of instructive significance for the prediction of the selectivity of MIPs.
Hydroxybenzoic acid, Molecular imprinting, Molecular recognition, Intramolecular hydrogen bond
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刘锋, Wei Xing Ma, Feng Liu, Ke An Li, Wen Chen, Shen Yang Tong
Analytica Chimica Acta 416(2000)191-196,-0001,():
-1年11月30日
This paper reports a simple and highly selective method for the preconcentration and separation of trace Hg(II) with aminopropylbenzoylazo-2-mercaptobenzothiazole bonded to silica gel(ABMBT SG).ABMBT SGwas tested for the selective extraction of Hg(II) and it showed an exchange capacity of 41.4μmol g−1. After preconcentration and separation using an ABMBT SG column, trace Hg(II) in environmental samples can be determined by UV–VIS spectrophotometry. The chromatographic column packed with ABMBT SG can be reused. The method is simple and efficient, and is applied to polluted soil (2μg g−1), incinerated biological material (9 or 18 ng g−1) and spiked natural water (5μg l−1).
Aminopropylbenzoylazo-2-mercaptobenzothiazole bonded to silica gel, Preconcentration, Separation, Hg(, II), , Environmental samples, UV-VIS spectrophotometry
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