郭雄
主要从事大骨节病的诊断、防治和病因发病机理研究。
个性化签名
- 姓名:郭雄
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学术头衔:
博士生导师
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学科领域:
内科学
- 研究兴趣:主要从事大骨节病的诊断、防治和病因发病机理研究。
郭雄,教授,博士生导师,男,1953年7月出生,享受国务院特殊津贴专家。1976年毕业于西安医科大学医疗系,留校到地方病研究所和卫生部“微量元素与地方病研究实验室”从事地方性骨关节病研究。现任西安交通大学医学院公共卫生系副主任,“环境与疾病相关基因”教育部重点实验室学术委员会委员。卫生部地方病标准委员会委员,中国诱变剂学会常务委员,中华医学会陕西省地方病学分会常务委员,中华医学会陕西省风湿病学分会常务委员,中华医学会西安市风湿病学分会副主任委员,西安市骨质疏松学学会副主任委员。1993年破格晋升副教授,1998年晋教授。1997年评为原西安医科大学跨世纪学术带头人,1998年、2001年评为陕西省优秀留学回国人员。指导博、硕士研究生30名。主要从事大骨节病的诊断、防治和病因发病机理研究,参加和承担国家“六五”至“八五”期间大骨节病科技攻关课题中的“小麦喷硒防治大骨节病效果研究”、“低硒与大骨节病关系的前瞻性研究”等多项研究课题,先后主持陕西省青年科学基金、陕西省科学基金、卫生部科研基金、国家自然科学基金和德国、美国国际合作基金项目10余项。2000年,2003年获2项国家自然科学基金,2004年获国家重大专项-重大疾病遗传资源收集项目子课题一项(中国大骨节病、克山病遗传资源的收集、保存与利用)、参加教育部科技创新重大项目《中国人群遗传相关疾病资源库及共享信息平台》培育项目。1996年赴德国Nurnberg-Erlangen大学分子实验医学研究所研修软骨细胞分子生物学实验技术,完成“大骨节病关节软骨胶原表型表达与软骨细胞异常分化”等2项课题。2001年11月-2002年4月为美国Rockefeller大学基因分析研究所客座教授。发表学术论文186篇,第一作者80篇。参加3部论著编写。参加第21届骨关节病国际学术讨论会(比利时)和第42届诺贝尔医学获奖者年会(德国)。作为主要成员参加的“低硒与大骨节病的关系”获1996年国际生物无机科学家协会Klaus Schwarz奖。合作完成的“沙依瓦克病”获1996年解放军总后勤部科学技术进步二等奖(第二获奖者),2003-2004年3项研究获陕西省、西安市科技进步二、三等奖。
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【期刊论文】大骨节病儿童及成人关节软骨中甲状旁腺激素相关肽的表达
郭雄, 张艳, 王波, 张增铁, 李幼芬, 许鹏
中国地方病学杂志,2002,21(6)436~438,-0001,():
-1年11月30日
目的 探索甲状旁腺激素相关肽(PTH rP)在大骨节病儿童及成人关节软骨中的表达及分布。方法 应用免疫组化方法比较正常儿童和成人与大骨节病儿童和成人关节软骨中PTH rP的表达。结果 ①大骨节病儿童关节软骨表层、中层、深层PTH rP阳性细胞表达率分别为30%、24% 和31%,中层、深层表达率比正常儿童明显增高(P<0.01);②大骨节病成人关节软骨表、中、深层PTH rP阳性细胞表达率分别为54%、46% 和16%,各层细胞阳性表达率均明显高于正常组(P<0.01)。③大骨节病儿童与成人比较各层软骨细胞PTH rP的阳性表达差异均有显著意义(P<0.01),表现为表、中层成人高于儿童,深层儿童高于成人。结论 大骨节病患者关节软骨中PTH rP阳性表达率高于正常对照。
大骨节病, 甲状旁腺激素相关肽, 关节软骨
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【期刊论文】大骨节病患者关节软骨细胞凋亡及相关调控因子Bcl-2表达分布的特点
郭雄, 杨王君, 张增铁, 曹春霞, 莫东旭
中国地方病学杂志,2002,21(5)341~343,-0001,():
-1年11月30日
目的 探讨大骨节病患者关节软骨细胞凋亡及相关调控因子Bcl-2表达分布的特点。方法 采用脱氧核糖核酸末端转移酶介导的脱氧核糖核酸缺口标记法(TUN EL氏法)检测凋亡阳性细胞,利用单克隆抗体免疫组化检测Bcl-2在关节软骨中的表达。结果 ①TUN EL法染色的大骨节病儿童关节软骨凋亡阳性细胞数较正常关节软骨增多,且主要分布在中层。②大骨节病儿童关节软骨Bcl-2的表达显著高于正常关节软骨中的表达,以表层最多,其次为中层;成人晚期大骨节病患者剥蚀的关节软骨表层Bcl-2的表达聚集在软骨细胞团中。结论 大骨节病患者关节软骨细胞凋亡及相关调控因子Bcl-2表达较正常人显著增多。
大骨节病, 细胞凋亡, 相关调控因子
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【期刊论文】THE PROSPECTIVE STUDY OF RELATIONSHIP BETWEEN LOW SELENIUM AND KASHIN-BECK D ISEASE*
郭雄, Guo Xiong Ding Dexiu Zeng Lingxia Yu Zhidao Chen Fengshi Zhao Zengqin Wang Jiuxing Bi Huayin
J XI' ANMEDUNIV 1999; 11(1):1~7,-0001,():
-1年11月30日
The relation ship of cause-result between low selen ium (Se) and Kashin-Beck disease (KBD) was probed by the prospective study of epidemiolog icalmethod with regarding low-Se as an exposure factor in this paper. 597 healthy children l ived in KBD areas with low, middle and high prevalence were divided in to the low-Se exposed group and the non-low-Se exposed group according to their Secontent in hair. The low-Se exposed group was divided in to three subgroups, such as Secontent in hair ≤ 110ng/g, 110ng/g<Secontent in hair≤150ng/g and 150ng/g<Secontent in hair ≤ 200ng/g, respectively. Six new cases of the total with KBD (incidence was 01574% person-year) were found in the low-Se exposed group during three years period of the investigation. No new case was found in the non low-Se exposed group. KBD incidence was notsign if icantly different between those two groups. Two new cases were found in children with Secontent in hair keptbelow 110ng/g during three years (inc idence: 1121% person-year). SMR in each group indicated that the new cases observed in the low-Se exposed group was remarkable lower than the new cases expected. It was not observed that the dose-respon serelation ship between low-Se and KBD, and was not supported that the low-Se was a predominant factor to cause KBD.
Kashin-Beck disease, selen ium, new case, prospective study
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郭雄, Ping Zhiguang Guo Xiong Geng Dong Kang Longli Zuo Hong Lai Jinaghua
ACAD J XJ TU 2005, 17(2):164-167,-0001,():
-1年11月30日
Objective To analyze the genetic polymorphism of 5 STR loci (D12S304, D12S313, D12S1583, D12S1640 and D12S1708) on chromosome 12 in Chinese Han population. Methods EDTA-anticoagulated blood specimens were collected from unrelated individuals of Chinese Han population in Shaanxi province. DNA samples were extracted with the Wizard Genomic DNA purification Kit and were amplified by polymerase chain reaction (PCR) technique. The PCR products were analyzed by ABI 3100 Genetic Analyzer. Results All 5 loci were in Hardy-Weinberg equilibrium. Allele and genotype frequencies, heterozygosity, power of discrimination, polymorphism information content, probability of paternity exclusion and matching probability of each locus were calculated in Excel 2002. Conclusion They are complexloci with lots of evenly distributed alleles and high heterozygosity in Chinese Han population. Thus they are informative polymorphicloci and valuable DNA marker which represents a superior alternative to many established STRs.
short tandem repeat (, STR), , genetic polymorphism, Hardy-Weinberg equilibrium, chromosome 12
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【期刊论文】ANALYSIS ON GENETIC POLYMORPHISM OF 6 STR LOCI ON CHROMOSOME 12 IN CHINESE HAN POPULATION
郭雄, Zuo Hong Guo Xiong Kang Longli Ping Zhiguang Wang Shijie Zhang Baodi Lai Jianghua Gen Dong
ACAD J XJ TU 2005, 17(2):160-164,-0001,():
-1年11月30日
Objective To analyze the genetic polymorphism of 6 STR loci (D12S358, D12S1675, D12S1663, D12S1697, D12S1725 and D12S1613) on chromosome 12 in Chinese Han population. Methods EDTA-blood specimens were collected from 153 unrelated individuals of Chinese Han population in Shaanxi province. Allele and genotype frequencies for the 6 STR loci were estimated and statistical parameters of polymorphism were calculated. Re sults 8 alleles and 18 genotypes, 10 alleles and 17 genotypes, 9 alleles and 15 genotypes, 12alleles and 29 genotypes, 12 alleles and 31 genotypes, 8 alleles and 11 genotypes were observed at D12S358, D12S1675, D12S1663, D12S1697, D12S1725 and D12S1613, respectively. No deviations of the observed allele frequency from Hardy-weinberg equilibrium expectations were found for any of these loci. The Heterozygotes of these 6 loci were 78.89%, 66.10%, 54.95%, 79.10%, 71.98% and 59.48%, respectively. It indicated the high genetic polymorphism of the loci in Chinese Han population. Conclusion The 6 STR loci belonged to the genetic marker system of high discriminutesation and high information in Chinese Han population and can be used in the study of gene-related diseases.
short tandem repeat, genetic polymorphism, Hardy-Weinberg equilibrium, chromosome 12
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【期刊论文】利用现代分子生物学技术深入研究大骨节病的分子发病机制
郭雄, 左弘
中国地方病学杂志2005,24(6)701~704,-0001,():
-1年11月30日
大骨节病, 软骨细胞, 基因, 分子生物学
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郭雄, Guo Xiong Mikko Lammi**, Thomas Aigner***, Pikko Lammi**, Silvia Vornehm**, Yu Zhidao Xiong Yongm in Klaus vonderMark**
J XI' ANMEDUNIV 2000, 12(2): 108~112,-0001,():
-1年11月30日
Objective According to the distr ibution of low selenium areas, low nutrition state of the residents and the affecting cartilage growth and articular cartilage of Kashin-Beck Disease (KBD), the chondrocyte differen tiation and differential expression of collagen types i, II and X in articular cartilage from Chinese mini-pigs treated with low selenium were investigated in order to gain in sight in to the effects of these condition son chondrocyte differen tiation in KBD cartilage. Me thods Eleven male juvenile mini-pigs, aged from 4 weeks to 6 weeks after birth, were divided in to 3 groups. The Secontent in the diet of the "low Se" group was 0.035mg/kg diet, and 0.175mg/kg diet in the control. For Se-supplemented group 0.390mg Na2SeO3/kg diet was added. The con tent of Sein blood was assayed at the beginning and at the end of each experiment. Samples of articular cartilage were taken from the right femur condylus, and collagen types I, II and "in articular cartilage were analyzed by immunohistochem istry and in situ hybridization. Results ①All cartilage samples from juven ilem in i-pigs fed with low selenium dietrevealed areduction in type "collagen mRNA expression in the hypertrophic chondrocytes as shown by in situ hybridization, and reduced type "collagen depos ition in the lower hypertrophic zone as shown by immunohistochem istry. ②Addition of selenium to the dietrestored the type" collagen to normal level. ③Type collagen was evenly distr ibuted over the entire articular cartilage in all experimental and controlgroups. Type collagen mRNA signals weremost prominent in the upper articular layer as well as in the hypertrophic zone in all groups. Type collagen express ion was restricted to the zone of endochondraloss if ication in all experimental groups and the control. Conclusion Low selen ium has an down-regulatoryrole on the syn thes is and deposition of collagen type "in hypertrophic chondrocytes in articular cartilage of mini-pigs. Supplement of the low Sediet with additional Serestored the signals of collagen type" to normal levels. These findings indicate that selenium deficiency may disturb chondrocyte differen tiation to hypertrophic cells in the growth plate, and worthy to be investigated further.
lowselenium, in situhybr idization, collagen expression, articular cartilage, mini-pig
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郭雄, 平智广, 王福歧, 王治文
中华流行病学杂志,2004,25(10)848~851,-0001,():
-1年11月30日
目的 分析大骨节病核心家庭的发病特点。方法 应用临床诊断搜集大骨节病核心家庭,根据父母患大骨节病情况将4938个核心家庭分为四种类型,结合病区类型,分析轻、中、重病区内不同核心家庭子代患病率及其家庭聚集性。结果 (1)核心家庭类型与病区类型的轻重有关;(2)中、重病区核心家庭子1代患病具有家庭聚集性;(3)仅双亲和父亲患大骨节病的核心家庭子1代具有明显的家庭聚集性;(4)双亲患大骨节病的核心家庭的子1代患病率明显高于单亲或双亲不患大骨节病的核心家庭。结论 大骨节病病区人群的患病除了与轻、中、重病区的类型有关,还可能与核心家庭双亲患大骨节病的情况有关。
大骨节病, 流行病学, 核心家庭, 聚集性
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