鞠熀先
分析化学的新技术、新方法和在生化分析中的应用
个性化签名
- 姓名:鞠熀先
- 目前身份:
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学术头衔:
博士生导师
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学科领域:
分析化学
- 研究兴趣:分析化学的新技术、新方法和在生化分析中的应用
鞠熀先1964年11月生。1986、1989和1992年获南京大学学士、硕士和博士学位,后留校任教,1993年聘为副教授,1999年聘为教授、博士生导师。1996至97年加拿大Montreal大学博士后。1999年11-12月为爱尔兰国立大学访问教授,2000年德国Potsdam大学和Münster大学客座教授,2002年曾在爱尔兰6所大学访问讲学,曾任巴黎国际传感器科学研讨会学术委会员委员,第二届国际传感器科学研讨会执行主席。现任南京大学分析化学教研室主任,《Sensors》副主编,《Analytical Letters》国际编委,《分析科学学报》、《药学学报》和《分析试验室》编委,中国化学会有机分析专业委员会委员,江苏省青年联合会常委,江苏省化学化工学会分析化学专业委员会主任,中国分析测试协会青年学术委员会副主任,分析化学山东省重点实验室学术委员会主任,重庆医科大学兼职教授。2003年获国家杰出青年科学基金。从事分析化学的教学与研究,对分析化学的新技术、新方法和在生化分析中的应用进行了系统、深入的研究,发表论文174篇(其中SCI刊物138篇,含Anal. Chem. 4篇,Adv. Mater. 1篇,Angew. Chem. Int. Ed. 1篇,Chem. Eur. J. 1篇,Electrophoresis 1篇,Frontiers in Bioscience 1篇,Langmuir 2篇,Inorg. Chem. 1篇, Biosensors & Bioelectronics 9篇,J. Immuno. Methods 4篇),申请专利6项(2项已授权)。合编专著2部,撰写中文专章2章、英文专章3章(均在出版中)。成果被SCI刊物他人引用500多次,在国内外产生了影响。曾获2001年江苏省青年科学家奖称号,2001年中国高校科学技术一等奖、1999年教育部科技进步三等奖、1995年国家教委科技进步三等奖和1996年中国化学会青年化学奖。
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主页访问
2856
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0
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成果阅读
421
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成果数
10
鞠熀先, Zhihui Dai, Songqin Liu, Huangxian Ju*, Hongyuan Chen
Biosensors and Bioelectronics 19(2004)861-867,-0001,():
-1年11月30日
The direct electrochemistry of hemoglobin (Hb) immobilized on a hexagonal mesoporous silica (HMS)-modified glassy carbon electrode was described. The interaction between Hb and the HMS was investigated using UV-Vis spectroscopy, FT-IR, and electrochemical methods. The direct electron transfer of the immobilized Hb exhibited two couples of redox peaks with the formal potentials of ?0.037 and ?0.232V in 0.1M (pH 7.0) PBS, respectively, which corresponded to its two immobilized states. The electrode reactions showed a surface-controlled process with a single proton transfer at the scan rate range from 20 to 200mV/s. The immobilized Hb retained its biological activity well and displayed an excellent response to the reduction of both hydrogen peroxide (H2O2) and nitrate (NO2-). Its apparent Michaelis-Menten constants for H2O2 and NO2-were 12.3 and 49.3μM, respectively, showing a good affinity. Based on the immobilization of Hb on the HMS and its direct electrochemistry, two novel biosensors for H2O2 and NO2-were presented. Under optimal conditions, the sensors could be used for the determination of H2O2 ranging from 0.4 to 6.0μM and NO2-ranging from 0.2 to 3.8μM. The detection limits were 1.86×10-9M and 6.11×10-7M at 3σ, respectively. HMS provided a good matrix for protein immobilization and biosensor preparation.
Biosensors, Hemoglobin, Hexagonal mesoporous silica, Glassy carbon electrode, Direct electron transfer, Hydrogen peroxide, Nitrate
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鞠熀先, Jianhua Zhao a*, Feng Yan a, Huangxian Ju b, *, Jinhai Tang c, Jianwei Qin c
Cancer Letters 204(2004)87-95,-0001,():
-1年11月30日
Serum vascular endothelial growth factor (VEGF) and endostatin levels were detected in 59 patients with breast cancer before surgery and at 3 weeks after surgery. Pre-operatively, their levels were significantly elevated and correlated with each other. Post-operatively, VEGF level decreased significantly and endostatin remained at a high level. Patients with both normalized VEGF and elevated endostatin following surgery had a lower risk of relapse than patients whose VEGF failed to normalize. Univariate and multivariate analyses showed a correlation between elevated VEGF level and short free-relapse survival. These findings suggest a new angiogenesis balance is formed in the patients after surgery and such a resultant balance may be beneficial for the prognosis of breast cancer, which deserves more extensive study.
Angiogenesis, Endostatin, Primary breast cancer, Vascular endothelial growth factor
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鞠熀先, Songqin Liu and Huangxian Ju*
Analyst, 2003, 128, 3-7,-0001,():
-1年11月30日
0.1 to 9.7 μmol dm-3 and a detection limit of 0.06 μmol dm-3 at 3σ. The amperometric determination of high concentrations of NO2-based on the irreversible reduction of NO could be performed at pH 4.0 with a linear range from 0.1 to 1.2 mmol dm-3. The surface of biosensor could be renewed quickly and reproducibly by a simple polish step. The biosensor has been used satisfactorily for nitrite determination in native water samples.
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鞠熀先, Dan Du a, Feng Yan b, Shengli Liu c, Huangxian Ju a, *
Journal of Immunological Methods 283(2003)67-75,-0001,():
-1年11月30日
We describe a novel electrochemical immunosensor for carbohydrate antigen 19-9 (CA19-9) based on the immobilization of CA19-9 with titania sol-gel on a graphite electrode (GE) by vapor deposition. The CA19-9 membrane was characterized using scanning electron microscopy and proved to be chemically clean, porous and homogeneous. The incubation of the immunosensor in a solution containing horseradish peroxidase (HRP)-labeled CA19-9 antibody led to the binding of HRP-labeled antibody with the immobilized antigen. The immobilized HRP catalyzed the oxidation of catechol by H2O2 and this provided a competitive method for the measurement of serum CA19-9. The response current decreased with increasing CA19-9 concentration in the incubation solution. The effects of pH, amount of HRP-labeled antibody, incubation time and temperature were explored to provide optimum analytical performance. Under optimal conditions, the current decrease of the immunosensor was proportional to CA19-9 concentrations in the range of 3-20 U/ml with a detection limit of 2.68 U/ml at a current decrease of 10%. The detection of CA19-9 in two serum samples obtained from clinically diagnosed patients with pancreatic carcinoma showed acceptable accuracy. The proposed immunosensor provides a new promising tool for the clinical immunoassay of CA19-9.
Immunosensor, Electrochemical sensors, Biosensors, Enzyme-labeled antibody, Carbohydrate antigen 19-9, CA19-9, Titania sol-gel, Pancreatic carcinoma
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鞠熀先, Jiuhong Yu, Songqin Liu, Huangxian Ju *
Biosensors and Bioelectronics 19(2003)509-514,-0001,():
-1年11月30日
A novel amperometric phenol sensor was constructed by immobilizing tyrosinase in a titania sol-gel matrix. The tyrosinase entrapped sol-gel film was obtained with a vapor deposition method, which simplified the traditional sol-gel process and avoided the shrinkage and cracking of conventional sol-gel-derived glasses. This matrix provided a microenvironment for retaining the native structure and activity of the entrapped enzyme and a very low mass transport barrier to the enzyme substrates. Phenol could be oxidized by dissolving oxygen in presence of immobilized tyrosinase to form a detectable product, which was determined at-150 mV without any mediator. The phenol sensor exhibited a fast response (less than 5 s) and sensitivity as high as 103μA/mM, which resulted from the porous structure and high enzyme loading of the sol-gel matrix. The linear range for phenol determination was from 1.2×10-7 to 2.6×10-4 M with a detection limit of 1.0×10-7M. The apparent Michaelis-Menten constant of the encapsulated tyrosinase was calculated to be (0.299±0.02) mM. The stability of the biosensor was also evaluated.
Sol-gel, Tyrosinase, Titania, Biosensors, Vapor deposition
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鞠熀先, Jiuhong Yu, Songqin Liu, Huangxian Ju*
Biosensors and Bioelectronics 19(2003)401-409,-0001,():
-1年11月30日
A novel amperometric glucose sensor was constructed by immobilizing glucose oxidase (GOD) in a titania sol-gel film, which was prepared with a vapor deposition method. The sol-gel film was uniform, porous and showed a very low mass transport barrier and a regular dense distribution of GOD. Titania sol-gel matrix retained the native structure and activity of entrapped enzyme and prevented the cracking of conventional sol-gel glasses and the leaking of enzyme out of the film. With ferrocenium as a mediator the glucose sensor exhibited a fast response, a wide linear range from 0.07 to 15mM. It showed a good accuracy and high sensitivity as 7.2 μAcm-2 mM-1. The general interferences coexisted in blood except ascorbic acid did not affect glucose determination, and coating Nafion film on the sol-gel film could eliminate the interference from ascorbic acid. The serum glucose determination results obtained with a flow injection analysis (FIA) system showed an acceptable accuracy, a good reproducibility and stability and indicated the sensor could be used in FIA determination of glucose. The vapor deposition method could fabricate glucose sensor in batches with a very small amount of enzyme.
Biosensors, Electrocatalysis, Sol-gel, Titania, Vapor deposition, Glucose, Glucose oxidase, Flow injection analysis
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鞠熀先, Zong Dai, †, Feng Yan, Jin Chen, † and Huangxian Ju*
Anal. Chem. 2003, 75, 5429-5434,-0001,():
-1年11月30日
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鞠熀先, Songqin Liu, Huangxian Ju*
Biosensors and Bioelectronics 19(2003)177-183,-0001,():
-1年11月30日
The direct electrochemistry of glucose oxidase (GOD) adsorbed on a colloidal gold modified carbon paste electrode was investigated. The adsorbed GOD displayed a pair of redox peaks with a formal potential of-(4499±1) mV in 0.1M pH 5.0 phosphate buffer solution. The response showed a surface-controlled electrode process with an electron transfer rate constant of (38.99±5.3)/s determined in the scan rate range from 10 to 100mV/s. GOD adsorbed on gold colloid nanoparticles maintained its bioactivity and stability. The immobilized GOD could electrocatalyze the reduction of dissolved oxygen and resulted in a great increase of the reduction peak current. Upon the addition of glucose, the reduction peak current decreased, which could be used for glucose detection with a high sensitivity (8.4μA/mM), a linear range from 0.04 to 0.28mM and a detection limit of 0.01mM at a signal-to-noise ratio of 3σ. The sensor could exclude the interference of commonly coexisted uric and ascorbic acid.
Biosensors, Carbon paste electrode, Glucose oxidase, Electrocatalysis, Colloidal gold, Glucose
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鞠熀先, Y.K. Ye a, J.H. Zhao b, F. Yan b, Y.L. Zhu a, H.X. Ju a, *
Biosensors and Bioelectronics 18(2003)1501-1508,-0001,():
-1年11月30日
Sequence-known short-stranded hepatitis B virus (HBV) DNA fragment (181 bps) was obtained by PCR method. The strategy for its electrochemical detection was designed by covalently immobilizing single-stranded HBV DNA on gold electrode surface via carboxylate ester as a linkage between 3'-hydroxy end of DNA and carboxyl group of thioglycolic acid (TGA) self-assembled monolayer. The hybridization reaction on surface was evidenced by electrochemical methods using ferrocenium hexafluorophosphate (FcPF6) as an electroactive indicator. The interactions of Fc+with single-stranded (ss) and double-stranded (ds) HBV DNA immobilized on TGA monolayer were studied. The difference between the responses of Fc+at ss-and ds-DNA/Au electrodes suggested that this hybridization biosensor could be conveniently used to monitor DNA hybridization with a high sensitivity. AC impedance and XPS techniques have been employed to characterize the immobilization of ss-DNA on the gold surface.
Hepatitis B virus, PCR, Self-assembled monolayer, Biosensors, Ferrocenium hexafluorophosphate
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鞠熀先, Huang-Xian Ju, a, *, Yong-Kang Ye, Jian-Hua Zhao, b and Yong-Lin Zhu a
Analytical Biochemistry 313(2003)255-261,-0001,():
-1年11月30日
A novel hepatitis B virus (HBV) DNA biosensor was developed by immobilizing covalently single-stranded HBV DNA fragments to a gold electrode surface via carboxylate ester to link the 3'-hydroxy end of the DNA with the carboxyl of the thioglycolic acid (TGA) monolayer. A short-stranded HBV DNA fragment (181 bp) of known sequence was obtained and amplified by PCR. The surface hybridization of the immobilized single-stranded HBV DNA fragment with its complementary DNA fragment was evidenced by electrochemical methods using [Os(bpy)2Cl2]+as a novel electroactive indicator. The formation of double-stranded HBV DNA on the gold electrode resulted in a great increase in the peak currents of [Os(bpy)2Cl2]+in comparison with those obtained at a bare or single-stranded HBV DNA-modified electrode. The mismatching experiment indicated that the surface hybridization was specific. The difference between the responses of [Os(bpy)2Cl2]+at single-stranded and double-stranded DNA/TGA gold electrodes suggested that the label-free hybridization biosensor could be conveniently used to monitor DNA hybridization with a high sensitivity. X-ray photoelectron spectrometry technique has been employed to characterize the immobilization of singlestranded HBV DNA on a gold surface.
Electrochemical DNA biosensor, Polymerase chain reaction, Hepatitis B virus, Hybridization, [Os(, bpy), 2Cl2]
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